Carbon Nanotube-Hydroxyapatite Composite for Bone Tissue Engineering and Their Interaction with Mouse Fibroblast L929 In Vitro

2017 ◽  
Vol 11 (3) ◽  
pp. 233-240 ◽  
Author(s):  
P. Khalid ◽  
V. B. Suman
Keyword(s):  
Tissue Engineering ◽  
Carbon Nanotube ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mouse Fibroblast ◽  
Hydroxyapatite Composite

scholarly journals A Proposed Fabrication Method of Novel PCL-GEL-HAp Nanocomposite Scaffolds for Bone Tissue Engineering Applications

2010 ◽  
Vol 19 (4) ◽  
pp. 096369351001900 ◽  
Author(s):  
A. Hamlekhan ◽  
M. Mozafari ◽  
N. Nezafati ◽  
M. Azami ◽  
H. Hadipour
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mechanical Test ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Fabrication Method ◽  
Engineering Applications ◽  
Poly Caprolactone

In this study, poly(∊-caprolactone) (PCL), gelatin (GEL) and nanocrystalline hydroxyapatite (HAp) was applied to fabricate novel PCL-GEL-HAp nanaocomposite scaffolds through a new fabrication method. With the aim of finding the best fabrication method, after testing different methods and solvents, the best method and solvents were found, and the nanocomposites were prepared through layer solvent casting combined with freeze-drying. Acetone and distillated water were used as the PCL and GEL solvents, respectively. The mechanical test showed that the increasing of the PCL weight through the scaffolds caused the improvement of the final nanocomposite mechanical behavior due to the increasing of the ultimate stress, stiffness and elastic modulus (8 MPa for 0% wt PCL to 23.5 MPa for 50% wt PCL). The biomineralization investigation of the scaffolds revealed the formation of bone-like apatite layers after immersion in simulated body fluid (SBF). In addition, the in vitro cytotoxity of the scaffolds using L929 mouse fibroblast cell line (ATCC) indicated no sign of toxicity. These results indicated that the fabricated scaffold possesses the prerequisites for bone tissue engineering applications.

Amine-functionalized Single-walled Carbon Nanotube/Polycaprolactone Electrospun Scaffold for Bone Tissue Engineering: in vitro Study

2019 ◽  
Vol 20 (9) ◽  
pp. 1869-1882 ◽  
Author(s):  
Hadi Tohidlou ◽  
Seyedeh Sara Shafiei ◽  
Shahsanam Abbasi ◽  
Mitra Asadi-Eydivand ◽  
Mehrnoosh Fathi-Roudsari
Keyword(s):  
Tissue Engineering ◽  
Carbon Nanotube ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
In Vitro Study ◽  
Electrospun Scaffold ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon ◽  
Amine Functionalized

Development of functionalized multi-walled carbon nanotube-based polysaccharide–hydroxyapatite scaffolds for bone tissue engineering

RSC Advances ◽  
2016 ◽  
Vol 6 (85) ◽  
pp. 82385-82393 ◽  
Author(s):  
R. Rajesh ◽  
Y. Dominic Ravichandran ◽  
M. Jeevan Kumar Reddy ◽  
Sung Hun Ryu ◽  
A. M. Shanmugharaj
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Carbon Nanotube ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Gellan Gum ◽  
Alp Activity ◽  
Multi Walled Carbon Nanotube ◽  
First Time

fMWCNT–amylopectin–HAP and fMWCNT–gellan gum–HAP were prepared and characterized and their in vitro cell proliferation and ALP activity were checked for the first time.

In Vitro Evaluation of Poly ε-Caprolactone/Hydroxyapatite Composite as Scaffolds for Bone Tissue Engineering with Human Bone Marrow Stromal Cells

2007 ◽  
Vol 342-343 ◽  
pp. 369-372 ◽  
Author(s):  
S.J. Heo ◽  
S.E. Kim ◽  
Yong Taek Hyun ◽  
D.H. Kim ◽  
Hyang Mi Lee ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Osteoblastic Differentiation ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Composite Scaffolds ◽  
Hydroxyapatite Composite

This study evaluated the potential of the PCL (poly -caprolactone)/HA(Hydroxyapatite) composite materials as a scaffold for bone regeneration. For this, we fabricated scaffolds utilizing salt leaching method. The PCL/HA composite scaffolds were prepared with various HA contents (20wt%, 40wt%, 60 wt %). To ensure the potential for the scaffolds, porosity tests were conducted along with SEM observations. The porosity decreased with the increase of the contents of HA particles. The porosity of the composite with the highest contents of HA was still adoptable (~85%). In addition, the PCL/HA composite scaffolds were evaluated for their ability of osteogenic differentiation with human bone marrow stromal cell (hBMSC) in vitro. Alkaline phosphatase (ALP) activity, markers for osteoblastic differentiation, and total protein contents were evaluated in hBMSCs following 14 days of cultivation. The addition of HA particles enhanced proliferation of hBMSC during the test. Also, the differentiation ability of the cells was increased as HA particles were added. In this study, we concluded that PCL/HA composite scaffolds has great potential as a scaffold for bone tissue engineering.

scholarly journals Excellency of Hydroxyapatite Composite Scaffolds for Bone Tissue Engineering

Biomaterials ◽  
2020 ◽  
Author(s):  
Mohammad Shariful Islam ◽  
Mohammad Abdulla-Al-Mamun ◽  
Alam Khan ◽  
Mitsugu Todo
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Surface Reactivity ◽  
Growth Factor Delivery ◽  
Composite Scaffolds ◽  
In Vivo Experiments ◽  
Hydroxyapatite Composite

The hydroxyapatite [HAp, Ca10(PO4)6(OH)2] has a variety of applications in bone fillers and replacements due to its excellent bioactivity and osteoconductivity. It comprises the main inorganic component of hard tissues. Among the various approaches, a composite approach using several components like biopolymer, gelatin, collagen, and chitosan in the functionalization of scaffolds with HAp has the prospective to be an engineered biomaterial for bone tissue engineering. HAp composite scaffolds have been developed to obtain a material with different functionalities such as surface reactivity, bioactivity, mechanical strength, and capability of drug or growth factor delivery. Several techniques and processes for the synthesis and fabrication of biocompatible HAp composite scaffolds suitable for bone regeneration are addressed here. Further, this chapter described the excellences of various HAp composite scaffolds used in in vitro and in vivo experiments in bone tissue engineering.

In vitro characterization of nanofibrous PLGA/gelatin/hydroxyapatite composite for bone tissue engineering

2010 ◽  
Vol 18 (12) ◽  
pp. 1195-1202 ◽  
Author(s):  
Jung Bok Lee ◽  
Sung Eun Kim ◽  
Dong Nyoung Heo ◽  
Il Keun Kwon ◽  
Byung-Joon Choi
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
In Vitro Characterization ◽  
Hydroxyapatite Composite
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