Cell Imaging via Multi-Electrode Array Using Microfluidic Chip

As a new medical detection method, electrical impedance tomography has been used in biology and medicine in recent years due to its advantages such as safety, non-invasiveness and radiation-free. In this paper, we designed a multi-electrode array microfluidic chip for the cell detection with electrical impedance tomography. The microfluidic chip structure design, material selection and processing technology are emphatically described. Finally, the feasibility of microfluidic chip for the cell with electrical impedance tomography is verified by simulations with different cell number and different electrodes excitation pattern.

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Imaging fascicular organization of peripheral nerves with fast neural Electrical Impedance Tomography (EIT)

10.1101/2020.06.04.133843 ◽

2020 ◽

Author(s):

Enrico Ravagli ◽

Svetlana Mastitskaya ◽

Nicole Thompson ◽

Francesco Iacoviello ◽

Paul R Shearing ◽

...

Keyword(s):

Electrical Impedance Tomography ◽

Peripheral Nerves ◽

Electrical Impedance ◽

Electrode Array ◽

Compound Action Potential ◽

Micro Computed Tomography ◽

Impedance Tomography ◽

Tissue Impedance ◽

Significant Difference ◽

Stimulation Of

Imaging of the compound action potential (CAP) in fascicles in peripheral nerves could help avoid side effects in neuromodulation by selective stimulation of identified fascicles. Existing methods have low resolution, limited imaging depth, or are invasive. We propose fast neural electrical impedance tomography (EIT), which allows fascicular CAP imaging with a high resolution of ∼200 μm, <1 ms. This uses a non-penetrating flexible cuff electrode array with 14 circumferential electrodes. This has been validated in rat sciatic nerve by comparison to micro-computed tomography (microCT) and histology with fluorescent dextran tracers (n=5). With EIT, there were reproducible localized changes in tissue impedance in response to stimulation of individual fascicles (tibial, peroneal and sural). The reconstructed EIT images corresponded to microCT scans and neural tracer histology, with significant separation between the fascicles (p<0.01), and no significant difference between techniques. The standard deviation from the mean fascicle position for EIT was 86 μm (6% of nerve diameter). This suggests fast neural EIT can reliably image the functional fascicular anatomy of the nerves and so aid selective neuromodulation.

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Imaging fascicular organization of rat sciatic nerves with fast neural electrical impedance tomography

Nature Communications ◽

10.1038/s41467-020-20127-x ◽

2020 ◽

Vol 11 (1) ◽

Author(s):

Enrico Ravagli ◽

Svetlana Mastitskaya ◽

Nicole Thompson ◽

Francesco Iacoviello ◽

Paul R. Shearing ◽

...

Keyword(s):

Electrical Impedance Tomography ◽

Electrical Impedance ◽

Electrode Array ◽

Micro Computed Tomography ◽

Impedance Tomography ◽

Tissue Impedance ◽

Cuff Electrode ◽

Nerve Cuff ◽

Compound Action Potentials ◽

Stimulation Of

AbstractImaging compound action potentials (CAPs) in peripheral nerves could help avoid side effects in neuromodulation by selective stimulation of identified fascicles. Existing methods have low resolution, limited imaging depth, or are invasive. Fast neural electrical impedance tomography (EIT) allows fascicular CAP imaging with a resolution of <200 µm, <1 ms using a non-penetrating flexible nerve cuff electrode array. Here, we validate EIT imaging in rat sciatic nerve by comparison to micro-computed tomography (microCT) and histology with fluorescent dextran tracers. With EIT, there are reproducible localized changes in tissue impedance in response to stimulation of individual fascicles (tibial, peroneal and sural). The reconstructed EIT images correspond to microCT scans and histology, with significant separation between the fascicles (p < 0.01). The mean fascicle position is identified with an accuracy of 6% of nerve diameter. This suggests fast neural EIT can reliably image the functional fascicular anatomy of the nerves and so aid selective neuromodulation.

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An electrical impedance tomography system for gynecological application GIT with a tiny electrode array

Physiological Measurement ◽

10.1088/0967-3334/33/5/849 ◽

2012 ◽

Vol 33 (5) ◽

pp. 849-862 ◽

Cited By ~ 10

Author(s):

Cherepenin V A ◽

Gulyaev Y V ◽

Korjenevsky A V ◽

Sapetsky S A ◽

Tuykin T S

Keyword(s):

Electrical Impedance Tomography ◽

Electrical Impedance ◽

Electrode Array ◽

Impedance Tomography ◽

Tomography System

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A Multitasking Electrical Impedance Tomography System Using Titanium Alloy Electrode

International Journal of Biomedical Imaging ◽

10.1155/2017/3589324 ◽

2017 ◽

Vol 2017 ◽

pp. 1-19 ◽

Cited By ~ 1

Author(s):

Abdalla Salama ◽

Amin Malekmohammadi ◽

Shahram Mohanna ◽

Rajprasad Rajkumar

Keyword(s):

Titanium Alloy ◽

Electrical Impedance Tomography ◽

Electrical Impedance ◽

Current System ◽

Electrode Array ◽

Alloy Electrode ◽

Impedance Tomography ◽

Tomography System ◽

Object Shapes ◽

Testing Tank

This paper presents a multitasking electrical impedance tomography (EIT) system designed to improve the flexibility and durability of an existing EIT system. The ability of the present EIT system to detect, locate, and reshape objects was evaluated by four different experiments. The results of the study show that the system can detect and locate an object with a diameter as small as 1.5 mm in a testing tank with a diameter of 134 mm. Moreover, the results demonstrate the ability of the current system to reconstruct an image of several dielectric object shapes. Based on the results of the experiments, the programmable EIT system can adapt the EIT system for different applications without the need to implement a new EIT system, which may help to save time and cost. The setup for all the experiments consisted of a testing tank with an attached 16-electrode array made of titanium alloy grade 2. The titanium alloy electrode was used to enhance EIT system’s durability and lifespan.

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