scholarly journals Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extraction and manipulation

PLoS Biology ◽  
2019 ◽  
Vol 17 (1) ◽  
pp. e3000107 ◽  
Author(s):  
Phil Oberacker ◽  
Peter Stepper ◽  
Donna M. Bond ◽  
Sven Höhn ◽  
Jule Focken ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
Magnetic Beads ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Open Platform

Design and Application of a High-Throughput Sample Processing Module Based on Magnetic Beads

2018 ◽  
Vol 10 (3) ◽  
pp. 320-328 ◽  
Author(s):  
Yile Fang ◽  
Yanqi Wu ◽  
Pei Liao ◽  
Zhu Chen ◽  
Hui Chen ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Magnetic Separation ◽  
High Throughput ◽  
Magnetic Beads ◽  
Performance Evaluations ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Dc Power ◽  
Processing Module

One of key challenges in diagnosis of infectious diseases is collecting a large quantity of purified nucleic acids from pathogens immediately. To address this challenge, a high-throughput magnetic separation module and a heating and vibrating module were developed to optimize the operation of biological sample preparation (like nucleic acid extraction) on our home-built liquid handing system. Both of the two modules have 4 working locations for Biomolecular Screening (BS) standard labware, and three functions (magnetic separation, heating and vibrating) were elaborately integrated into the two modules. Each module has its own core control circuit and a unique interface port containing a 24 V DC power supply connector and a CAN bus communication connector. When equipping with the two modules, the home-built liquid handing system transformed into an integrated automatic sample preparation workstation. Performance evaluations were carried out on the two modules and finally a nucleic acid extraction of human's whole blood was carried out on the workstation. Results showed that the two modules could cooperate well with the liquid handing system, and the workstation exhibited its ability for high-throughput sample preparation.

scholarly journals Evaluation of an Automated High-Throughput Liquid-Based RNA Extraction Platform on Pooled Nasopharyngeal or Saliva Specimens for SARS-CoV-2 RT-PCR

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 615
Author(s):  
Allen Wing-Ho Chu ◽  
Cyril Chik-Yan Yip ◽  
Wan-Mui Chan ◽  
Anthony Chin-Ki Ng ◽  
Dream Lok-Sze Chan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
Rna Extraction ◽  
Nasopharyngeal Swab ◽  
Acid Extraction ◽  
Rt Pcr ◽  
Nucleic Acid Extraction ◽  
Suitable Alternative ◽  
Pcr Inhibitors ◽  
Pcr Targeting

SARS-CoV-2 RT-PCR with pooled specimens has been implemented during the COVID-19 pandemic as a cost- and manpower-saving strategy for large-scale testing. However, there is a paucity of data on the efficiency of different nucleic acid extraction platforms on pooled specimens. This study compared a novel automated high-throughput liquid-based RNA extraction (LRE) platform (PHASIFYTM) with a widely used magnetic bead-based total nucleic acid extraction (MBTE) platform (NucliSENS® easyMAG®). A total of 60 pools of nasopharyngeal swab and 60 pools of posterior oropharyngeal saliva specimens, each consisting of 1 SARS-CoV-2 positive and 9 SARS-CoV-2 negative specimens, were included for the comparison. Real-time RT-PCR targeting the SARS-CoV-2 RdRp/Hel gene was performed, and GAPDH RT-PCR was used to detect RT-PCR inhibitors. No significant differences were observed in the Ct values and overall RT-PCR positive rates between LRE and MBTE platforms (92.5% (111/120] vs 90% (108/120]), but there was a slightly higher positive rate for LRE (88.3% (53/60]) than MBTE (81.7% (49/60]) among pooled saliva. The automated LRE method is comparable to a standard MBTE method for the detection of SAR-CoV-2 in pooled specimens, providing a suitable alternative automated extraction platform. Furthermore, LRE may be better suited for pooled saliva specimens due to more efficient removal of RT-PCR inhibitors.

scholarly journals NEATTILL: A simplified procedure for nucleic acid extraction from arrayed tissue for TILLING and other high-throughput reverse genetic applications

Plant Methods ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 3 ◽  
Author(s):  
Yellamaraju Sreelakshmi ◽  
Soni Gupta ◽  
Reddaiah Bodanapu ◽  
Vineeta Chauhan ◽  
Mickey Hanjabam ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
Acid Extraction ◽  
Reverse Genetic ◽  
Nucleic Acid Extraction ◽  
Simplified Procedure

Functional Magnetite Nanoparticle: A Review on the Particles Lysis and Nucleic Acid Separation

2021 ◽  
Vol 33 ◽  
pp. 13-27
Author(s):  
Puspita Nurlilasari ◽  
Camellia Panatarani ◽  
Mia Miranti ◽  
Savira Ekawardhani ◽  
Ferry Faizal ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Magnetite Nanoparticles ◽  
Magnetic Beads ◽  
Functional Materials ◽  
Cell Lysis ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Coating Materials ◽  
Magnetite Nanoparticle ◽  
Separation Cell

The functional magnetite nanoparticles are one of the most important functional materials for nucleic acid separation. Cell lysis and magnetic separation are two essential steps involve in optimizing nucleic acid extraction using the magnetic beads method. Many coating materials, coupling agents, chemical cell lysis, and several methods have been proposed to produce the specific desired properties for nucleic acid extraction. The important properties, such as biocompatibility, stability, linking ability, hydrophobicity, and biodegradable, were considered. The appropriate coating material of magnetite core and coupling agent are necessary to give biomolecules a possibility to link with each other through chemical conjugation. In this review, progress in functional magnetite nanoparticles to optimize the high binding performance in nucleic acid extraction is discussed.

Inexpensive, High Throughput Microplate Format for Plant Nucleic Acid Extraction: Suitable for Multiplex Southern Analyses of Transgenes

Crop Science ◽  
2005 ◽  
Vol 45 (5) ◽  
pp. 1985-1989 ◽  
Author(s):  
L. Flagel ◽  
J. R. Christensen ◽  
C. D. Gustus ◽  
K. P. Smith ◽  
P. M. Olhoft ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

scholarly journals High-throughput HTLV-1 proviral DNA detection system using a nucleic acid extraction robot and real-time PCR detection.

2001 ◽  
Vol 47 (3) ◽  
pp. 378-383 ◽  
Author(s):  
Chieko Matsumoto ◽  
Rieko Shiozawa ◽  
Shigeki Mitsunaga ◽  
Akiko Ichikawa ◽  
Rika Ishiwatari ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
High Throughput ◽  
Real Time Pcr ◽  
Detection System ◽  
Dna Detection ◽  
Pcr Detection ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Proviral Dna

scholarly journals A bifurcated continuous field-flow fractionation (BCFFF) chip for high-yield and high-throughput nucleic acid extraction and purification

Lab on a Chip ◽  
2019 ◽  
Vol 19 (22) ◽  
pp. 3853-3861 ◽  
Author(s):  
Chenguang Zhang ◽  
Gongchen Sun ◽  
Satyajyoti Senapati ◽  
Hsueh-Chia Chang
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
High Yield ◽  
Acid Extraction ◽  
Field Flow Fractionation ◽  
Nucleic Acid Extraction ◽  
Isolation And Purification ◽  
Extraction And Purification ◽  
Flow Fractionation ◽  
Continuous Field

We report a new Bifurcated Continuous Field-Flow Fractionation (BCFFF) microfluidic chip for isolation and purification of nucleic acids from blood plasma with high and concentration-independent yield. The platform is ideal for isolation and quantification of small miRNAs.

Effects of diverse materials-based methods on DNA extraction for Clostridium difficle from stool samples

2019 ◽  
Vol 9 (5) ◽  
pp. 509-516 ◽  
Author(s):  
Ziqi Xiao ◽  
Gaojian Yang ◽  
Deng Yan ◽  
Song Li ◽  
Zhu Chen ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Extraction ◽  
Magnetic Beads ◽  
Diagnostic Technique ◽  
Proteinase K ◽  
Molecular Diagnostic ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Spin Column ◽  
Stool Samples

Nosocomial infections, including Clostridium difficile infection (CDI), and their fatality rates have increased in the past few decades. Despite emerging molecular diagnostic technologies with rapid, accurate outcomes, nucleic acid extraction from stool samples remains the first limiting step before downstream applications. Commercial nucleic acid extraction kits greatly decrease labor and time requirements, and also provide nucleic acid preparations with higher quality and purity for enzyme digestion analysis or genotyping. The magnetic bead based technique is a novel method compared with the conventional spin-column method, and currently has widespread use in nucleic acid extraction. We evaluated five DNA extraction kits with magnetic beads using materials with various properties (particle size, concentration of magnetic beads, grinding beads) and reagents (proteinase K, lysozyme, isopropanol, and absolute ethanol) to determine the cost, hands-on time, number of essential operations, and quality and purity of the DNA preparations, compared with those obtained using the QIAamp Fast DNA Stool Mini Kit. The six DNA extraction kits yielded A260/280 ratios ranging from 0.85 to 1.9 (average 1.57), and concentrations from 3.70 to 108.09 ng/μL (average 34.64 ng/μL). All the DNA samples had acceptable downstream application effects, except for those obtained using the TIANGEN Magnetic Soil and Stool DNA Kit. However, gel electrophoresis analysis of the DNA samples resulted in a light strip on the gel, indicating that the proteinaceous contaminant may not have been removed completely. A rapid and accurate molecular diagnostic technique could allow for more suitable treatment and prognosis outcomes for inpatients, depending, in large part, on the quality and purity of DNA preparations, which are frequently neglected. Our study focused on the quality of commercial kits with a primary focus on the treatment of stool samples and molecular diagnostic applications.

scholarly journals Parallel RNA extraction using magnetic beads and a droplet array

Lab on a Chip ◽  
2015 ◽  
Vol 15 (4) ◽  
pp. 1059-1065 ◽  
Author(s):  
Xu Shi ◽  
Chun-Hong Chen ◽  
Weimin Gao ◽  
Shih-hui Chao ◽  
Deirdre R. Meldrum
Keyword(s):  
Nucleic Acid ◽  
Magnetic Beads ◽  
Rna Extraction ◽  
Lab On A Chip ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Droplet Array

Nucleic acid extraction is a necessary step for most genomic/transcriptomic analyses, but it often requires complicated mechanisms to be integrated into a lab-on-a-chip device.

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