Role of Tumor Necrosis Factor and Interleukin-1 in Endotoxin-Induced Middle Ear Effusions

1997 ◽  
Vol 106 (8) ◽  
pp. 633-639 ◽  
Author(s):  
Jiri Prazma ◽  
Rudolph J. Triana ◽  
Steven S. Ball ◽  
C. G. Dean Dais ◽  
Harold C. Pillsbury
Keyword(s):  
Tumor Necrosis Factor ◽  
Middle Ear ◽  
Rat Model ◽  
Tumor Necrosis ◽  
Otitis Media With Effusion ◽  
Interleukin 1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Novel Approach ◽  
Necrosis Factor

In a rat model, we investigated the role of tumor necrosis factor (TNF) and interleukin-1 (IL-1) in endotoxin-induced middle ear effusions (MEEs). After the eustachian tube was obstructed, the middle ear was transtympanically injected with 35μL of either 1) 1 mg/ mL lipopolysaccharide (LPS); 2) LPS and 100 μg TNF binding protein (TNFbp); 3) LPS and 1 μg IL-1 receptor antagonist (IL-1ra); or 4) LPS, TNFbp, and IL-1ra. Every 2 hours, the fluid within the middle ear was collected, and the quantity of albumin in the fluid, an index of vascular leakage, was determined by enzyme-linked immunosorbent assay. After 6 hours, the middle ear was fixed for histologic analysis. The TNFbp significantly attenuated vascular extravasation into the middle ear. The IL-1ra did not significantly alter effusion development. These results indicate that TNF, but not IL-1, is a mediator of LPS-induced MEE. Therefore, TNFbp may represent a novel approach to the treatment of otitis media with effusion.

Tumor necrosis factor-α and interleukin-1 β levels in recurrent and persistent otitis media with effusion

2002 ◽  
Vol 126 (4) ◽  
pp. 417-422 ◽  
Author(s):  
Sertac Yetiser ◽  
Bulent Satar ◽  
Atilla Gumusgun ◽  
Faruk Unal ◽  
Yalcin Ozkaptan
Keyword(s):  
Tumor Necrosis Factor ◽  
Otitis Media ◽  
Tumor Necrosis ◽  
Otitis Media With Effusion ◽  
Interleukin 1 ◽  
Tertiary Referral Center ◽  
Adenoid Tissue ◽  
Tnf Α ◽  
Factor Α ◽  
Necrosis Factor

OBJECTIVE: Based on interleukin (IL)-1β and tumor necrosis factor (TNF)-α levels in effusions, our goals were to specify either recurrent or persistent otitis media with effusion (OME) is a mid stage in the development of chronic disease and to identify the factors that have an influence on cytokine levels. STUDY DESIGN: Samples from groups with recurrent (n = 15) and persistent (n = 39) OME were essayed for IL-1 β and TNF-α. Children were also grouped with respect to age, sex, quality of effusion, and the presence of pharyngeal adenoid tissue. SETTING: Tertiary referral center. RESULTS: In recurrent and persistent OME groups, IL-1β was higher than TNF-α ( P < 0.01). IL-β was higher in recurrent OME than in persistent OME ( P < 0.05). CONCLUSION: Recurrent OME seems to be closer to the chronic stage of the disease relative to persistent OME in terms of higher IL-1 β levels. Each exacerbation of acute disease in recurrent otitis media is likely to be mediated by IL-1 β. SIGNIFICANCE: We were able to clarify that recurrent OME is a stage that occurs before chronic OME. Therefore, the prevention of acute attacks in recurrent disease would also impede long-term damage to the middle ear.

Pathogenic role of interleukin 1 and tumor necrosis factor in autoimmune endocrine diseases

1988 ◽  
Vol 9 (5) ◽  
pp. 72-74 ◽  
Author(s):  
Klaus Bendtzen ◽  
Åse Krogh Rasmussen ◽  
Karine Bech ◽  
Ulla Feldt-Rasmussen ◽  
Karsten Buschard
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Interleukin 1 ◽  
Pathogenic Role ◽  
Endocrine Diseases ◽  
Necrosis Factor

scholarly journals Jiawei Xianglian Decoction (JWXLD), a Traditional Chinese Medicine (TCM), Alleviates CPT-11-Induced Diarrhea in Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Jinhua Lu ◽  
Zechen Lin ◽  
Siyu Huang ◽  
Yiwei Shen ◽  
Jing Jiang ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Interleukin 1 ◽  
Active Form ◽  
Mucosal Injury ◽  
Enzyme Linked Immunosorbent Assay ◽  
Uridine Diphosphate ◽  
Necrosis Factor Alpha ◽  
Interleukin 7 ◽  
Necrosis Factor

Irinotecan (CPT-11) is used for therapy of various cancers. However, it has several serious adverse reactions such as diarrhea which is caused by SN-38, the active form of CPT-11. This study aimed to evaluate the effectiveness of Jiawei xianglian decoction (JWXLD), which has been widely used for the treatment of diarrhea in China. In this study, a mouse model with delayed diarrhea was generated by CPT-11. Real-time PCR and enzyme-linked immunosorbent assay (ELISA) were performed to explore intestinal microflora and inflammatory cytokine. Hematoxylin and eosin (H&E) staining was used to analyze tissue morphology. We found that 0.12, 0.23, and 0.46 g JWXLD significantly reduced the severity of CPT-11-induced diarrhea. The levels of Lactobacillus (Lacto) and Bifidobacterium (Bifid) were significantly downregulated by CPT-11, and these effects can be reversed by JWXLD treatment. Furthermore, JWXLD was observed to decrease the activity of β-glucuronidase (β-GD). Histopathological data showed that CPT-11 induced severe intestinal mucosal injury, which was characterized as grade 6, and JWXLD significantly alleviated the injury. In addition, CPT-11 increased the productions of tumor necrosis factor-alpha (TNF-α), tumor necrosis factor-beta (TNF-β), interleukin-6 (IL-6), and interleukin-1 (IL-1), but decreased interleukin-15 (IL-15), interleukin-7 (IL-7), and uridine diphosphate-glucuronosyltransferase 1A1 (UGT1A1). In conclusion, JWXLD can counteract these effects caused by CPT-11 treatment. JWXLD could alleviate CPT-11-induced diarrhea.

Role of Complement, Interleukin-1 and Tumor Necrosis Factor in a Local Shwartzman-Like Reaction

2015 ◽  
pp. 69-78 ◽  
Author(s):  
Henry Z. Movat ◽  
Clement E. Burrowes ◽  
Myron I. Cybulsky ◽  
Charles A. Dinarello
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Interleukin 1 ◽  
Necrosis Factor
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