scholarly journals Bilirubin Measured on a Blood Gas Analyser: A Suitable Alternative for Near-Patient Assessment of Neonatal Jaundice?

2001 ◽  
Vol 38 (5) ◽  
pp. 533-540 ◽  
Author(s):  
Michael Peake ◽  
Beryl Mazzachi ◽  
Andrew Fudge ◽  
Renze Bais
Keyword(s):  
Whole Blood ◽  
Neonatal Jaundice ◽  
Total Bilirubin ◽  
Blood Gas ◽  
Patient Assessment ◽  
Suitable Alternative ◽  
Other Substances ◽  
Multi Wavelength ◽  
Synthetic Controls ◽  
Primary Standards

The reliability of a recently released total bilirubin assay for a blood gas analyser was assessed in two Australian hospital laboratories. The instrument computes total bilirubin concentration from multi-wavelength absorbance measurements of undiluted whole blood or plasma. Performance of the Radiometer ABL 735 blood gas analyser bilirubin method (software version 3.6) was compared with a proven Roche diazo method for Hitachi analysers, calibrated using primary standards prepared from NIST SRM 916a bilirubin. Acceptable bilirubin results were found over a wide concentration range for most neonatal samples of whole blood or plasma. For adult specimens, bilirubin results were approximately 10% lower on the blood gas analyser. Within-run imprecision (whole blood) was <2·5%, between-day imprecision (synthetic controls) <1·0%, and the bilirubin assay for both whole blood and plasma was linear to 1000μmol/L. Using sampling options from 35 μL to 195μL, bilirubin results differed by less than 3%, with a 95 μL syringe option producing the highest results. We conclude that the Radiometer ABL 735 bilirubin assay is suitable for near-patient assessment of neonatal jaundice using whole blood, thus eliminating the need for sample centrifugation. Verification using laboratory methods can be used when required. A positive correction of approximately 10% is required for adult specimens to conform with Hitachi results (SRM 916a calibration), possibly due to the optical characteristics of the higher proportion of conjugated bilirubin and other substances present in most adult samples.

scholarly journals Reliability of the determination of whole-blood oxygen affinity by means of blood-gas analyzers and multi-wavelength oximeters.

1989 ◽  
Vol 35 (5) ◽  
pp. 773-777 ◽  
Author(s):  
G Kwant ◽  
B Oeseburg ◽  
W G Zijistra
Keyword(s):  
Whole Blood ◽  
Carbon Monoxide Poisoning ◽  
Oxygen Affinity ◽  
Reference Interval ◽  
Accurate Method ◽  
Blood Gas ◽  
Oxygen Dissociation ◽  
Multi Wavelength ◽  
Dissociation Curves

Abstract Determination of the oxygen affinity of human whole blood with the help of blood-gas analyzers and multi-wavelength oximeters is compared with an accurate method for recording hemoglobin oxygen dissociation curves (Clin Chem 1982;28:1287-92). P50 (oxygen tension at half saturation; So2 = 50%) and Hill's n (delta log [So2/(1-So2)]/delta log Po2) were determined in blood of 24 healthy donors. Three slightly different procedures were used for determination of P50 on the basis of Po2, pH, Pco2, and So2 measured with each of four different blood-gas analyzer/oximeter combinations. These methods were not able to discriminate between high and low values for P50 within the normal reference interval, but never failed to detect the high oxygen affinity of blood stored for 12 days, reflected in low values of P50. The methods thus proved suitable for detecting clinically significant deviations in oxygen affinity such as occur in patients with, e.g., abnormal hemoglobins, anemias, or carbon monoxide poisoning. Determination of Hill's n by these methods did not produce useful results.

Reliability of Total Bilirubin Measurements in Whole Blood from Preterm Neonates Using a Blood Gas Analyzer

2016 ◽  
Vol 62 (11/2016) ◽  
Author(s):  
Toshihiko Nambara ◽  
Yoshinori Katayama ◽  
Masahiro Enomoto ◽  
Shin Kikuchi ◽  
Atsuko Takei ◽  
...  
Keyword(s):  
Whole Blood ◽  
Total Bilirubin ◽  
Preterm Neonates ◽  
Blood Gas ◽  
Gas Analyzer ◽  
Blood Gas Analyzer

scholarly journals Assessment of Donkey (Equus asinus africanus) Whole Blood Stored in CPDA-1 and CPD/SAG-M Blood Bags

Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 133
Author(s):  
Isabella Oliveira Barros ◽  
Rejane Santos Sousa ◽  
Marcondes Dias Tavares ◽  
Renato Otaviano Rêgo ◽  
Paulo Ricardo Firmino ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Whole Blood ◽  
Blood Collection ◽  
Blood Gas ◽  
Blood Cell Count ◽  
Animal Blood ◽  
Equus Asinus ◽  
Citrate Phosphate Dextrose ◽  
Citrate Phosphate

Hemotherapy using whole blood and its components is being increasingly used in veterinary therapy. Since it is important to store animal blood while maintaining acceptable hematological, blood gas, and biochemical characteristics, increasing our knowledge of available technologies for strategic blood storage is imperative. Thus, we aimed to assess the hematological, blood gas, and biochemical changes in donkey whole blood using blood bags with two different types of storage agents. Eight adult healthy male donkeys were used; 900 mL of blood was collected from each, with 450 mL stored in citrate-phosphate-dextrose and adenine bags (CPDA-1) and 450 mL stored in bags containing citrate-phosphate-dextrose, adenine, mannitol, and sodium chloride (CPD/SAG-M). Both bags were kept refrigerated between 1 and 6 °C for 42 days. Blood samples were removed from the bags eight times (T): T0 (immediately after blood collection), T1, T3, T7, T14, T21, T35, and T42 (1, 3, 7, 14, 21, 35 and 42 days after storage). Hematological, blood gas, biochemical, and microbiological parameters were assessed. The CPDA-1 bags had a higher packed cell volume when compared to CPD/ SAG-M. The red blood cell count reduced by around 19% in both the bags due to hemolysis, which was confirmed by an increase in plasma hemoglobin. The white blood cell count; pH; concentrations of glucose, sodium, bicarbonate, and 2,3 diphosphoglycerate were reduced in both bags. Meanwhile, pO2, pCO2, lactate dehydrogenase, and levels of potassium increased in the CPDA-1 and CPD/SAG-M bags. Blood bags were efficient for the storage of donkey blood for up to 42 days.

Is the Fluorometric Method for Blood Bilirubin Accurate?

PEDIATRICS ◽  
1980 ◽  
Vol 66 (4) ◽  
pp. 653-654
Author(s):  
George Cembrowski ◽  
Carl C. Garber
Keyword(s):  
Data Analysis ◽  
Linear Regression ◽  
Correlation Coefficient ◽  
Whole Blood ◽  
Total Bilirubin ◽  
Regression Line ◽  
Method Comparison ◽  
Fluorometric Method ◽  
Accuracy And Precision ◽  
Comparison Data

The article by Brown et al1 interested us greatly. Direct fluorometric measurement of whole blood bilirubin, if accurate and precise, will help in the care of the jaundiced neonate. The accuracy and precision of this technique, however, cannot be readily assessed from the authors' data. Consider the comparison of the fluorometric method to the Jendrassik-Grof method for total bilirubin. To assess accuracy, the authors use the correlation coefficient, r, and the slope of the linear regression line through the method comparison data. There are two problems with this data analysis.

Temperature correction of blood-gas and pH measurements.

1983 ◽  
Vol 29 (11) ◽  
pp. 1877-1885 ◽  
Author(s):  
E R Ashwood ◽  
G Kost ◽  
M Kenny
Keyword(s):  
Experimental Verification ◽  
Whole Blood ◽  
Temperature Correction ◽  
Clinical Usefulness ◽  
Blood Gas ◽  
Ph Measurements ◽  
Theoretical Derivation ◽  
Clinical Interpretation ◽  
Induced Changes

Abstract We critically review formulas for temperature correction of pH, pCO2, and pO2 measurements in whole blood and the clinical usefulness of these formulas. We discuss both the theoretical derivation and experimental verification of temperature-induced changes. We recommend when to use and when not to use these formulas, based upon the clinical interpretation of these assays.

A simplified method for determining the P50 of blood

1975 ◽  
Vol 38 (6) ◽  
pp. 1140-1142 ◽  
Author(s):  
B. W. Kirk ◽  
M. B. Raber ◽  
K. R. Duke
Keyword(s):  
Oxygen Saturation ◽  
Oxygen Content ◽  
Whole Blood ◽  
Normal Subjects ◽  
Blood Gas ◽  
Inexpensive Method ◽  
Ph Measurements ◽  
Simplified Method ◽  
Very High ◽  
Made In

A method to determine the P50 of whole blood is described using a modified American Optical reflectance oximeter, pump, and membrane tonometer, together with PO2, PCO2, and pH measurements in a standard blood gas machine. Determinations of P50 were made in 66 patients and normal subjects and in two situations where P50 was very low and very high. The results were compared to oxygen saturations calculated from measured oxygen content. The directly determined oxygen saturation agreed with the assumed saturation of 50 per cent in the oximeter within 0.5 per cent. The apparatus appears to be a simple and relatively inexpensive method to obtain P50 as long as blood carboxyhemoglobin or methemoglobin contents are not elevated.

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