scholarly journals Negative interference by rheumatoid factor in alpha-fetoprotein chemiluminescent microparticle immunoassay

2016 ◽  
Vol 54 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Hui Wang ◽  
Xiaohui Bi ◽  
Lei Xu ◽  
Yirong Li
Keyword(s):  
Hepatitis B ◽  
Rheumatoid Factor ◽  
Hepatitis B Surface Antigen ◽  
Alpha Fetoprotein ◽  
Serum Samples ◽  
Negative Interference ◽  
Positive Serum ◽  
Serum Alpha Fetoprotein ◽  
Chemiluminescent Microparticle Immunoassay ◽  
Paramagnetic Microparticles

Background Rheumatoid factor causes positive interference in multiple immunoassays. Recently, negative interference has also been found in immunoassays in the presence of rheumatoid factor. The chemiluminescent microparticle immunoassay is widely used to determine serum alpha-fetoprotein. However, it is not clear whether the presence of rheumatoid factor in the serum causes interference in the chemiluminescent microparticle immunoassay of alpha-fetoprotein. Methods Serum alpha-fetoprotein was determined using the ARCHITECT alpha-fetoprotein assay. The estimation of alpha-fetoprotein recovery was carried out in samples prepared by diluting high-concentration alpha-fetoprotein serum with rheumatoid factor-positive or rheumatoid factor-negative serum. Paramagnetic microparticles coated with hepatitis B surface antigen–anti-HBs complexes were used to remove rheumatoid factor from the serum. Results The average recovery of alpha-fetoprotein was 88.4% and 93.8% in the rheumatoid factor-positive and rheumatoid factor-negative serum samples, respectively. The recovery of alpha-fetoprotein was significantly lower in the rheumatoid factor-positive serum samples than in the rheumatoid factor-negative serum samples. In two of five rheumatoid factor-positive samples, a large difference was found (9.8%) between the average alpha-fetoprotein recoveries in the serially diluted and initial recoveries. Fourteen rheumatoid factor-positive serum samples were pretreated with hepatitis B surface antigen-anti-HBs complex-coated paramagnetic microparticles. The alpha-fetoprotein concentrations measured in the pretreated samples increased significantly. Conclusions It was concluded that the alpha-fetoprotein chemiluminescent microparticle immunoassay is susceptible to interference by rheumatoid factor, leading to significantly lower results. Eliminating the incidence of negative interference from rheumatoid factor should be an important goal for immunoassay providers. In the meantime, laboratorians must remain alert to the negative interference by rheumatoid factor, and in some cases, pretreat rheumatoid factor-positive samples with blocking or absorbing reagents.

Serum alpha fetoprotein in 714 mentally retarded carriers of hepatitis B surface antigen

1988 ◽  
Vol 4 (2) ◽  
pp. 181-184 ◽  
Author(s):  
G. Lohiya ◽  
S. Lohiya ◽  
T. Fischer ◽  
H. Nguyen ◽  
T. Vuu ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Mentally Retarded ◽  
Alpha Fetoprotein ◽  
Serum Alpha Fetoprotein

Quantitation of hepatitis B surface antigen by an automated chemiluminescent microparticle immunoassay

2004 ◽  
Vol 115 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Matsuo Deguchi ◽  
Naoko Yamashita ◽  
Masanori Kagita ◽  
Seishi Asari ◽  
Yoshinori Iwatani ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Chemiluminescent Microparticle Immunoassay

Reversible increase in serum alpha-fetoprotein content associated with hepatic dysfunction during chemotherapy for seminoma.

1986 ◽  
Vol 4 (1) ◽  
pp. 41-45 ◽  
Author(s):  
J L Grem ◽  
D L Trump
Keyword(s):  
Hepatitis B ◽  
Germ Cell ◽  
Hepatic Dysfunction ◽  
Tumor Regression ◽  
Germ Cell Tumors ◽  
Alpha Fetoprotein ◽  
Response To Treatment ◽  
Beta Hcg ◽  
B Virus ◽  
Serum Alpha Fetoprotein

Serial monitoring of the serum content of the beta subunit of human chorionic gonadotropin (beta hCG) and alpha-fetoprotein (alpha FP) is useful in the initial staging of germ cell tumors and assessing the response to treatment. An increase in either marker during or following treatment almost always heralds disease progression and indicates the need for additional therapy. We report two patients in whom substantial increases in the serum content of AFP occurred during chemotherapy for advanced seminoma. Hepatic dysfunction was present in both patients; in one patient, a chronic carrier of hepatitis B virus, the liver dysfunction was associated with reactivation of hepatitis B manifested by anicteric hepatitis and hepatitis B e antigen positivity. Marked tumor regression had occurred in both patients, and chemotherapy was discontinued in spite of the elevated alpha FP level. The alpha FP content in the serum gradually returned to normal, and hepatic dysfunction resolved. Both patients remain free of disease 15 and 17 months following the last chemotherapy treatment. These cases illustrate that hepatic dysfunction and alpha FP production may occur during chemotherapy and that increases in serum alpha FP content must be interpreted with caution since the elevated alpha FP level does not always indicate progression of germ cell tumors.

SERO PREVALENCE OF TRANSFUSION TRANSMITTED INFECTIONS (TTI) IN VOLUNTARY BLOOD DONORS IN A TERTIARY CARE HOSPITAL AT SRINAGAR

2021 ◽  
Vol 5 (5) ◽  
Author(s):  
Nadia Shafi Chickan ◽  
Altaf Hussain Mir ◽  
Masaratt-ul Gani ◽  
Shazia Handoo ◽  
Farooq Ahmad Ganie
Keyword(s):  
Health Care ◽  
Hepatitis C ◽  
Hepatitis B ◽  
Blood Donors ◽  
Hepatitis B Surface Antigen ◽  
Tertiary Care ◽  
Care Facility ◽  
Health Care Facility ◽  
Care Hospital ◽  
Serum Samples

Introduction: Blood transfusion plays an vital role in patients management and is live saving in my instances. it plays a pivotal role as a specialized modality of treatment and saves millions of lives worldwide each year and reduces morbidity. Stringent screening of blood not only ensure safe supply of blood and blood products, but also gives us idea about prevalence of ttis among general population Objective: to determine the serroperavalance of tti among blood donors in lalded hospital Materials and methods: A retrospective study was carried out over a period of 3 years from January 2017 to December 2019.serum samples were screened for hepatitis B surface antigen ,antibodies to human immunodeficiency virus Type 1 and 2 ,hepatitis c virus and syphilis in department of transfusion medicine was carried out Result: The result shows that 55 (3.72%) of the blood donors had TTIs. The prevalence of Hepatitis B, Hepatitis C, Malaria, Syphilis and Human immunodeficiency viruses (HIV), was found to be 31(0.21%), 23 (0.15%), 01 (0.0.006%), 01 (0.006%) and 02 (0.0.23%) respectively. None were positive for malaria. Conclusion: Transfusion Transmittable Infections pose a threat to patients admitted in any health care facility and if not carefully screened for will cause significant morbidity. Careful screening of all donor blood units in the blood bank of a hospital is absolutely necessary to prevent any untoward incidence of TTI in patients admitted to a health care facility Keywords: Transfusion, Sero-Prevalence, Donors, Transfusion Transmissible Infections.

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