The article presents an assessment of three physical factors of the work environment, i.e. noise, illumination and microclimate in selected rooms of a hospital. The rooms were selected in cooperation with occupational safety and health services. Quantities characterizing noise, illumination and microclimate were measured in the operating room and in the sterilization and pathomorphology rooms. For this purpose, the measurement methods included in the standards were applied. The pilot study showed that noise limits were exceeded in terms of annoyance and that there is a need to improve illumination in the operating room, in the sterilization room and in the histological laboratory. In the operating and in the sterilization rooms, the requirement of thermal comfort was met with the assumption of low physical activity of employees, while in the pathomorphology room, changes are necessary.