Structural and Histochemical Observations of Fluorotic Enamel Matrix

1979 ◽  
Vol 58 (2_suppl) ◽  
pp. 1028-1029 ◽  
Author(s):  
M. Triller
Keyword(s):  
Light Microscopy ◽  
Enamel Matrix ◽  
Human Teeth ◽  
Irregular Pattern ◽  
The Matrix

The matrix of fluorotic enamel is studied by S.E.M. and histochemically by light microscopy. Observations are made on fluorotic human teeth and on rat incisors in which fluorosis has been produced by daily injections of NaF. The enamel sheaths appear enlarged and irregular, rich in stainable proteins, while glycocomponents of the matrix are less dense. A relationship is suggested between those alterations and the further irregular pattern of mineralization of fluorotic enamel.

Matrix-Mineral Relationships-A. Morphologist's Viewpoint

1979 ◽  
Vol 58 (2_suppl) ◽  
pp. 922-929 ◽  
Author(s):  
M.U. Nylen
Keyword(s):  
Organic Material ◽  
Crucial Role ◽  
Crystal Surface ◽  
Organic Matrix ◽  
The Other ◽  
Enamel Matrix ◽  
Initial Cell ◽  
Cell Product ◽  
Ultrastructural Morphology ◽  
The Matrix

The literature on the ultrastructural morphology of the enamel matrix and its relationship to the crystals is reviewed. Two morphological entities of the matrix are discussed: One is the so-called stippled material which may be the initial cell product; the other, variously described as fibrillar, lamellar, tubular or helical, is thought by many to play a crucial role in nucleation and orientation of the crystals. A number of observations, however, suggest that the latter structures form secondarily to the crystals and that in reality they represent organic material adsorbed to the crystal surface and maintained as independent structures upon removal of the mineral. The need for additional studies is stressed including systematic studies of interactions between constituents of the organic matrix and the apatite crystals.

Cartilage anomaly (can); a new mutant gene in the mouse

Development ◽  
1971 ◽  
Vol 25 (1) ◽  
pp. 21-31
Author(s):  
D. R. Johnson ◽  
Jean M. Wise
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Recessive Gene ◽  
Mutant Gene ◽  
Cartilaginous Matrix ◽  
The Matrix

Cartilage anomaly (symbol can) is a recessive gene in the mouse producing achondroplasia. Abnormal mice die at about 10 days after birth. Light microscopy reveals a systemic deficiency in the cartilaginous matrix. Electron microscopy suggests that the collagen of the matrix is normal and that the mucopolysaccharide component is reduced.

scholarly journals Identification of a protein-containing enamel matrix layer which bridges with the dentine–enamel junction of adult human teeth

2012 ◽  
Vol 57 (12) ◽  
pp. 1585-1594 ◽  
Author(s):  
Vladimir Dusevich ◽  
Changqi Xu ◽  
Yong Wang ◽  
Mary P. Walker ◽  
Jeff P. Gorski
Keyword(s):  
Enamel Matrix ◽  
Human Teeth ◽  
Adult Human ◽  
Matrix Layer

Presence of an enamel matrix protein derivative on human teeth following periodontal surgery

2002 ◽  
Vol 6 (3) ◽  
pp. 183-187 ◽  
Author(s):  
Anton Sculean ◽  
Péter Windisch ◽  
Tibor Keglevich ◽  
Beata Fabi ◽  
Eva Lundgren ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Enamel Matrix ◽  
Periodontal Surgery ◽  
Human Teeth ◽  
Enamel Matrix Protein

scholarly journals The presence and possible functions of the matrix metalloproteinase collagenase activator protein in developing enamel matrix

1989 ◽  
Vol 264 (3) ◽  
pp. 917-920 ◽  
Author(s):  
P K DenBesten ◽  
L M Heffernan ◽  
B V Treadwell ◽  
B J Awbrey
Keyword(s):  
Matrix Metalloproteinase ◽  
Enamel Matrix ◽  
Activator Protein ◽  
The Matrix

The developing enamel matrix contains mostly amelogenins, which are hydrophobic proline-rich proteins. During amelogenesis, the amelogenins are presumably hydrolysed and removed from the enamel. Recently a number of metalloproteinases that may be important in amelogenesis have been identified in zymograms of the developing enamel matrix. In the present study an antibody specific for the matrix metalloproteinase collagenase activator protein (CAP) was characterized and used to identify this metalloproteinase in enamel. Immunoblotting showed that the CAP proteinase was present in the enamel matrix. Immunohistochemistry confirmed that the proteinase is localized in the enamel matrix, most specifically along the dentino-enamel junction. Purified CAP was found to hydrolyse amelogenin protein. Possible functions of the proteinase in the enamel matrix are discussed.

Effects of enamel matrix proteins on tissue formation along the roots of human teeth

2005 ◽  
Vol 40 (2) ◽  
pp. 158-167 ◽  
Author(s):  
Dieter D. Bosshardt ◽  
Anton Sculean ◽  
Peter Windisch ◽  
Bjarni E. Pjetursson ◽  
Niklaus P. Lang
Keyword(s):  
Matrix Proteins ◽  
Tissue Formation ◽  
Enamel Matrix ◽  
Enamel Matrix Proteins ◽  
Human Teeth

scholarly journals Исследование структурных особенностей дентина методами микротомографии и просвечивающей электронной микроскопии

2020 ◽  
Vol 90 (9) ◽  
pp. 1449
Author(s):  
Т.С. Аргунова ◽  
Ж.В. Гудкина ◽  
М.Ю. Гуткин ◽  
Д.В. Зайцев ◽  
А.Е. Калмыков ◽  
...  
Keyword(s):  
Structural Features ◽  
Human Teeth ◽  
Dentinal Tubules ◽  
X Ray ◽  
Transmission Electron ◽  
Secondary Cracks ◽  
Mode Ii Crack ◽  
The Matrix ◽  
Dentin Matrix ◽  
Natural Composite

Dentin of human teeth is a natural composite material with a layered hierarchical structure; it is formed by micro- and nanostructures whose complex relations obey the laws not yet fully understood. In order to understand the effect of the dentin structure on its mechanical properties, a study of dentin samples prepared from human molars and subjected to a uniaxial compression was made. Synchrotron X-ray microtomography and transmission electron microscopy were employed to investigate the elements of dentin matrix and the tubules which cross the matrix. Single structural features based on the interaction of the elements, as well as the properties of the dentinal tubules, which can stop crack propagation have been investigated. A model is proposed for calculating the conditions of stopping of a mode II crack by generation of secondary cracks caused by the stresses over the surfaces of the tubules.

Enamel Matrix: Structural Proteins

1979 ◽  
Vol 58 (2_suppl) ◽  
pp. 773-781 ◽  
Author(s):  
J.D. Termine ◽  
D.A. Torchia ◽  
K.M. Conn
Keyword(s):  
Molecular Weight ◽  
Matrix Protein ◽  
Extraction Methods ◽  
Resonance Spectroscopy ◽  
Segmental Motion ◽  
Enamel Matrix ◽  
Molecular Weights ◽  
The Matrix ◽  
Enamel Protein ◽  
Bovine Enamel

Cell-free, fetal bovine enamel tissue was examined intact by high resolution. 13C Fourier transform, nuclear magnetic resonance spectroscopy. Two types of protein chains were observed under these conditions, one exhibiting rapid mobility and accounting for approximately two-thirds of the enamel matrix, while the other exhibited restricted or anisotropic segmental motion and accounted for the remaining third of the matrix. Sequential extraction of this fetal enamel under non-degradative conditions with dissociative solvents yielded two biochemically distinct populations of matrix protein. As expected, the bulk of the matrix consisted of proline-rich amelogenins, although the SDS-gel electrophoresis molecular weights for these proteins were somewhat higher than those reported using other extraction methods. Approximately fifteen percent of the total matrix consisted of much higher molecular weight phosphoproteins (46,000 - 72,000 daltons) whose amino acid composition closely resembled that reported for mature enamel protein. These high molecular weight proteins were tightly bound to the fetal enamel apatite crystallites.

scholarly journals Are there structural alterations in the enamel organ of offspring of rats with alloxan-induced diabetes mellitus?

2003 ◽  
Vol 14 (3) ◽  
pp. 162-167 ◽  
Author(s):  
Yara Teresinha Corrêa Silva-Sousa ◽  
Luiz Cesar Peres ◽  
Milton César Foss
Keyword(s):  
Structural Defects ◽  
Deciduous Teeth ◽  
Enamel Organ ◽  
Developmental Defect ◽  
Enamel Matrix ◽  
Enamel Hypoplasia ◽  
Human Teeth ◽  
Structural Alterations ◽  
Future Studies ◽  
Diabetic Mothers

Enamel hypoplasia is the most common developmental defect of human teeth that may be seen in deciduous teeth of babies born to diabetic women. In the present experimental study, we analyzed the enamel organ of the mandibular incisors of the offspring of rats with alloxan-induced diabetes. By light microscopy, no alterations could be found in the enamel organ of rats born to diabetic mothers compared to normal ones, except in one case. In contrast, significant differences were detected with computer-aided morphometry. In the rats born to treated and untreated diabetic mothers, there was thinning of the enamel matrix and of the ameloblasts and the nuclear area of the latter was smaller. In the rats born to treated diabetic mothers, the nuclei of the ameloblasts were more elliptical and there was enlargement of the interstitial area of the stellate reticulum. These results indicate that there are structural defects in the enamel organ of rats born to mothers with alloxan-induced diabetes which could induce the enamel hypoplasia observed by scanning electron microscopy and which may reflect the metabolic alterations seen in this condition. Future studies are needed to determine whether these effects are transitory or permanent.

Enamel Matrix and Crystals — Chicken and Eggs?

1979 ◽  
Vol 58 (2_suppl) ◽  
pp. 943-949 ◽  
Author(s):  
S.A. Leach
Keyword(s):  
Present Author ◽  
General Rule ◽  
The Other ◽  
Enamel Matrix ◽  
Research Areas ◽  
The Matrix

This discussion is presented as an attempt to interrelate the matrix and mineral in terms of our existing knowledge as presented at this Symposium, and to relate it, where possible, to what is being accomplished in allied disciplines and research areas elsewhere. The present author is fully aware of the difficulties involved in this task and of his own inadequacies in attempting to perform it. As a general rule, with perhaps the morphologists being the exception to it, the matrix and crystal components have been investigated independently of each other. This, of course, is quite justifiable, as the presence of one can often interfere with the investigation of the other, and each needs to be examined initially in as pure a state as possible.

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