scholarly journals Are there structural alterations in the enamel organ of offspring of rats with alloxan-induced diabetes mellitus?

2003 ◽  
Vol 14 (3) ◽  
pp. 162-167 ◽  
Author(s):  
Yara Teresinha Corrêa Silva-Sousa ◽  
Luiz Cesar Peres ◽  
Milton César Foss
Keyword(s):  
Structural Defects ◽  
Deciduous Teeth ◽  
Enamel Organ ◽  
Developmental Defect ◽  
Enamel Matrix ◽  
Enamel Hypoplasia ◽  
Human Teeth ◽  
Structural Alterations ◽  
Future Studies ◽  
Diabetic Mothers

Enamel hypoplasia is the most common developmental defect of human teeth that may be seen in deciduous teeth of babies born to diabetic women. In the present experimental study, we analyzed the enamel organ of the mandibular incisors of the offspring of rats with alloxan-induced diabetes. By light microscopy, no alterations could be found in the enamel organ of rats born to diabetic mothers compared to normal ones, except in one case. In contrast, significant differences were detected with computer-aided morphometry. In the rats born to treated and untreated diabetic mothers, there was thinning of the enamel matrix and of the ameloblasts and the nuclear area of the latter was smaller. In the rats born to treated diabetic mothers, the nuclei of the ameloblasts were more elliptical and there was enlargement of the interstitial area of the stellate reticulum. These results indicate that there are structural defects in the enamel organ of rats born to mothers with alloxan-induced diabetes which could induce the enamel hypoplasia observed by scanning electron microscopy and which may reflect the metabolic alterations seen in this condition. Future studies are needed to determine whether these effects are transitory or permanent.

scholarly journals Indirect veneer treatment of anterior maxillary teeth with enamel hypoplasia

2010 ◽  
Vol 43 (3) ◽  
pp. 157
Author(s):  
Devi Eka Juniarti
Keyword(s):  
Economic Status ◽  
Tooth Surface ◽  
Developmental Defect ◽  
Enamel Matrix ◽  
Enamel Hypoplasia ◽  
Anterior Teeth ◽  
White Spots ◽  
Dental Hospital ◽  
The Aesthetic ◽  
Gingival Irritation

Background: Nowadays, aesthetic rehabilitation becomes a necessity. It is affected by patient’s background, especially career, social and economic status. The aesthetic abnormality of anterior teeth i.e discoloration, malposition and malformation can affect patient’s appearance, especially during smile. These dental abnormalities, as a result, can decrease patient’s performance. Dental malformation, for instance, can be caused by developmental tooth defect, such as enamel hypoplasia. Enamel hypoplasia is a developmental defect caused by the lack of matrix amount which leads to thin and porous enamel. Enamel hypoplasia can also be caused by matrix calcification disturbance starting from the formation and development of enamel matrix causing defect and permanent changes which can occur on one or more tooth. Purpose: The aim of the study is to improve dental discoloration and tooth surface texture on anterior maxillary teeth with enamel hypoplasia by using indirect veneer with porcelain material. Case: A 20 years-old woman with enamel hypoplasia came to the Dental Hospital, Faculty of Dentistry Airlangga University. The patient wanted to improve her anterior maxillary teeth. It is clinically known that there were some opaque white spots (chalky spotted) and porous on anterior teeth’s surface. Case management: Indirect veneer with porcelain material had been chosen as a restoration treatment which has excellent aesthetics and strength, and did not cause gingival irritation. As a result, the treatment could improve the confidence of the patient, and could also make their function normal. Conclusion: Indirect veneer is an effective treatment, which can improve patient’s appearance and self confidence.Latar belakang: Saat ini perbaikan estetik menjadi suatu kebutuhan. Kebutuhan akan estetik dipengaruhi latar belakang penderita, terutama karir, status sosial dan ekonomi. Hal ini disebabkan, kelainan estetik seperti diskolorasi, malposisi, malformasi, dapat mempengaruhi penampilan penderita terutama saat tersenyum. Kelainan gigi tersebut pada akhirnya dapat memperburuk penampilan penderita. Malformasi gigi dapat disebabkan oleh kelainan pada masa perkembangan gigi, misalnya hipoplasia enamel. Hipoplasia enamel adalah kelainan perkembangan yang disebabkan sedikitnya matriks enamel sehingga terjadi ketipisan dan porusnya enamel. Hipoplasia enamel dapat disebabkan gangguan kalsifikasi matriks saat pembentukan dan perkembangan matriks enamel, kerusakan dan perubahan permanen ini dapat melibatkan satu atau beberapa gigi. Tujuan: Tujuan perawatan ini memperbaiki diskolorasi dan tekstur permukaan gigi depan rahang atas akibat hipoplasia enamel menggunakan veneer indirek berbahan porselen. Kasus: Seorang penderita wanita 20 tahun dengan hipoplasia enamel datang ke Rumah Sakit Gigi dan Mulut Pendidikan Fakultas Kedokteran Gigi Universitas Airlangga. Penderita ingin memperbaiki gigi depan rahang atas yang secara klinis tampak bercak putih opak seperti kapur dan porus pada permukan labial gigi. Tatalaksana kasus: Veneer indirek dengan bahan porselen dipilih sebagai restorasi oleh karena segi estetik, kekuatan dan tidak mengiritasi ginggiva. Restorasi ini dapat meningkatkan kepercayaan diri penderita dan memperbaiki fungsi gigi. Kesimpulan: Veneer indirek merupakan perawatan yang efektif, dapat meningkatkan penampilan dan kepercayan diri penderita.

Autoradiography of Incorporated Leucine-H3 in the Enamel Matrix and Enamel Organ of the Upper Incisor of the Rat

1964 ◽  
Vol 04 (02) ◽  
pp. 186-192
Author(s):  
Leonel Costacurta
Keyword(s):  
Amino Acid ◽  
Blood Vessels ◽  
Tooth Germ ◽  
Longitudinal Section ◽  
Equal Length ◽  
Enamel Organ ◽  
Enamel Matrix ◽  
Distal Extremity ◽  
Old Rats ◽  
Thin Band

SummaryDental germs of the upper incisors of six-days old rats were studied for the uptake of leucine-H3 by different layers of the enamel organ in correlation to the various stages of the development of enamel.The longitudinal section of the tooth germ was divided into 15 zones of about equal length in order to facilitate the description and interpretation of results. Autoradiographic images of the histologic preparations from rats sacrificed 30 minutes, 1 hour, 1 day and 3 days after the injection were made. The strongest reactions were observed in dental germs of rats sacrificed 1 hour, and particularly one day, after the leucine-H3 injection.The uptake of this compound by the enamel matrix increases progressively up to the young enamel and then decreases to the distal extremity; the greatest quantity of this labeled amino-acid was observed in the primary and young enamel. The reactions were present in the transitional enamel only along a thin band close to the dentine-enamel junction.In the enamel organ leucine-H3 incorporation was greatest in the three layers, the zones corresponding to primary and young enamel. In zones corresponding to transitional enamel, the inner epithelium showed a small quantity, and the stellate reticulum a blackening only in its superficial part, were the blood vessels reach the enamel organ.

Matrix and Mineral Changes in Developing Enamel

1979 ◽  
Vol 58 (2_suppl) ◽  
pp. 871-882 ◽  
Author(s):  
C. Robinson ◽  
H.D. Briggs ◽  
P.J. Atkinson ◽  
J.A. Weatherell
Keyword(s):  
Mineral Content ◽  
Inorganic Ions ◽  
Organic Matrix ◽  
Small Samples ◽  
Enamel Organ ◽  
Enamel Matrix ◽  
Subsequent Loss ◽  
Matrix Components ◽  
Significant Concentration ◽  
Mineral Changes

An investigation of the changes taking place in the enamel and the enamel organ during enamel development has been carried out by analyzing small samples of tissue dissected from developing incisors of rat and bovine incisors. Observations showed that the synthesis of the enamel matrix and its subsequent loss were associated chiefly with a change in the major matrix components. This consisted of a selective loss of amelogenin components prior to secondary mineralization. Before this loss, some increase in the proportion of smaller molecular weight components suggested the possibility of limited breakdown. Even at the earliest stages examined, significant concentration of mineral ions was present. This increased steeply after most of the organic matrix had been removed. The Ca/P ratio of this mineral was constant throughout development. The concentration of minor inorganic ions (F, Mg and CO3) decreased as the tissue developed and a tendency was observed for certain ions (F, 32PO4) to penetrate and concentrate in the enamel, apparently as a consequence of the lost matrix being replaced by water, just prior to the steep increase in mineral content of the tissue.

Tetracycline syrups and children’s teeth

1984 ◽  
Vol 22 (14) ◽  
pp. 55-56
Keyword(s):  
At Risk ◽  
In Utero ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Enamel Hypoplasia ◽  
Yellow Colour ◽  
Paediatric Patients ◽  
Short Course

It has been known for over 25 years that treatment with oral tetracyclines can permanently stain children’s teeth1 yet these drugs are still needlessly being prescribed for children. In 1982 over 75,000 prescriptions for a liquid tetracycline preparation were dispensed, most of which were probably for children; up to one third of paediatric patients have been affected,2–6 although the proportion has fallen over the last 10 years.7 Even a short course of tetracycline can stain both the permanent3,8 and deciduous teeth9 a disfiguring greyish-brown or yellow colour. Children are at risk from the 14th week in utero, when calcification of deciduous teeth begins, to their 7th year when calcification of the permanent teeth is complete. Whether tetracyclines produce enamel hypoplasia10 or promote caries11 is disputed.12

Structural and Histochemical Observations of Fluorotic Enamel Matrix

1979 ◽  
Vol 58 (2_suppl) ◽  
pp. 1028-1029 ◽  
Author(s):  
M. Triller
Keyword(s):  
Light Microscopy ◽  
Enamel Matrix ◽  
Human Teeth ◽  
Irregular Pattern ◽  
The Matrix

The matrix of fluorotic enamel is studied by S.E.M. and histochemically by light microscopy. Observations are made on fluorotic human teeth and on rat incisors in which fluorosis has been produced by daily injections of NaF. The enamel sheaths appear enlarged and irregular, rich in stainable proteins, while glycocomponents of the matrix are less dense. A relationship is suggested between those alterations and the further irregular pattern of mineralization of fluorotic enamel.

scholarly journals Prenatal and neonatal variables associated with enamel hypoplasia in deciduous teeth in low birth weight preterm infants

2007 ◽  
Vol 15 (6) ◽  
pp. 518-523 ◽  
Author(s):  
Kátia Maria Dmytraczenko Franco ◽  
Sérgio Roberto Peres Line ◽  
Maria Valeriana Leme de Moura-Ribeiro
Keyword(s):  
Birth Weight ◽  
Low Birth Weight ◽  
Preterm Infants ◽  
Deciduous Teeth ◽  
Enamel Hypoplasia

scholarly journals Identification of a protein-containing enamel matrix layer which bridges with the dentine–enamel junction of adult human teeth

2012 ◽  
Vol 57 (12) ◽  
pp. 1585-1594 ◽  
Author(s):  
Vladimir Dusevich ◽  
Changqi Xu ◽  
Yong Wang ◽  
Mary P. Walker ◽  
Jeff P. Gorski
Keyword(s):  
Enamel Matrix ◽  
Human Teeth ◽  
Adult Human ◽  
Matrix Layer

scholarly journals A probable genetic origin for pit defects on the molars of Paranthropus robustus

2018 ◽  
Author(s):  
Ian Towle ◽  
Joel D. Irish
Keyword(s):  
Rapid Evolution ◽  
Modern Human ◽  
Amelogenesis Imperfecta ◽  
Primate Species ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Enamel Hypoplasia ◽  
Genetic Origin ◽  
Posterior Teeth ◽  
Anterior Teeth

AbstractWe report the frequencies of linear enamel hypoplasia (LEH) and, specifically, pitting enamel hypoplasia (PEH) defects in the teeth of Paranthropus robustus, for comparison with four other South African hominin species and three extant nonhuman primate species. Unlike LEH, the lesser known PEH is characterized by multiple circular depression defects across a tooth crown and is often difficult to interpret in terms of developmental timing and etiology. Teeth in all samples were examined macroscopically with type, position and number of defects recorded. Frequencies of teeth with LEH vary among hominin species, but the differences in PEH are considerable. That is, P. robustus has much higher rates of pitting defects, with 47% of deciduous teeth and 14% of permanent teeth affected, relative to 6.7% and 4.3%, respectively, for all other hominin teeth combined; none of the extant primate samples evidence comparable rates. The defects on P. robustus molars are unlike those in other species, with entire crowns often covered in small circular depressions. The PEH is most consistent with modern human examples of amelogenesis imperfecta. Additionally, the defects are: 1) not found on anterior teeth, 2) uniform in shape and size, and 3) similar in appearance/severity on all molars. A possible reason for this form of PEH is as a side effect of selection on another phenotype that shares the same coding gene(s), i.e., a genetic origin. Recent research on the ENAM gene provides one such possibility. Paranthropus likely underwent rapid evolution in the ENAM loci, with changes in this gene contributing to larger posterior teeth and thicker enamel. This same gene is associated with amelogenesis imperfecta; therefore, pleiotropy effects, relating to high selection on this gene during Paranthropus evolution, could have yielded this unique condition.

scholarly journals Isolation and culture of dental pulp stem cells from permanent and deciduous teeth

2019 ◽  
Vol 35 (4) ◽  
Author(s):  
Shagufta Naz ◽  
Farhan Raza Khan ◽  
Raheela Rahmat Zohra ◽  
Sahreena Salim Lakhundi ◽  
Mehwish Sagheer Khan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Tooth Pulp ◽  
Human Teeth ◽  
Creative Commons ◽  
Calcified Tissue

Objective: To isolate dental pulp mesenchymal stem cells (MSCs) from non-infected human permanent and deciduous teeth. Methods: It was an in-vitro experimental study. Human teeth were collected from 13 apparently healthy subjects including nine adults and four children. After decoronation dental pulps were extirpated from teeth and cultured via explant method in a stem cell defined media. Data was analyzed by descriptive statistics. Results: As above MSCs emerged exhibiting fibroblast-like morphology. In vitro culture was positive for 100% (9/9) and 75% (3/4) of the permanent and deciduous teeth respectively. First cell appeared from deciduous teeth pulp in 10±6.2 days while permanent teeth pulp took 12.4±3.7 days. Together, 26.6±3.6 and 24.5±3.5 days were required for permanent and deciduous tooth pulp stem cells to be ready for further assays. Conclusions: The protocol we developed is easy and consistent and can be used to generate reliable source of MScs for engineering of calcified and non-calcified tissue for regenerative medicine approaches. doi: https://doi.org/10.12669/pjms.35.4.540 How to cite this:Naz S, Khan FR, Zohra RR, Lakhundi SS, Khan MS, Mohammed N, et al. Isolation and culture of dental pulp stem cells from permanent and deciduous teeth. Pak J Med Sci. 2019;35(4):---------. doi: https://doi.org/10.12669/pjms.35.4.540 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Sign in / Sign up

Export Citation Format

Share Document