RNA sequencing identified novel target genes for Adansonia digitata in breast and colon cancer cells

Adansonia digitata exhibits numerous beneficial effects. In the current study, we investigated the anti-cancer effects of four different extracts of A. digitata (polar and non-polar extracts of fruit powder and fibers) on the proliferation of human colon cancer (HCT116), human breast cancer (MCF-7), and human ovarian cancer (OVCAR-3 and OVCAR-4) cell lines. RNA sequencing revealed the influence of the effective A. digitata fraction on the gene expression profiles of responsive cells. The results indicated that only the polar extract of the A. digitata fibers exhibited anti-proliferative activities against HCT116 and MCF-7 cells, but not ovarian cancer cells. Moreover, the polar extract of the fibers resulted in the modulation of the expression of multiple genes in HCT116 and MCF-7 cells. We propose that casein kinase 2 alpha 3 ( CSNK2A3) is a novel casein kinase 2 ( CSNK2) isoform in HCT116 cells and report, for the first time, the potential involvement of FYVE, RhoGEF, and PH domain-containing 3 ( FGD3) in colon cancer. Together, these findings provide evidence supporting the anti-cancer potential of the polar extract of A. digitata fibers in this experimental model of breast and colon cancers.

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Knockdown of ICB-1 gene enhanced estrogen responsiveness of ovarian and breast cancer cells

Endocrine Related Cancer ◽

10.1677/erc-09-0095 ◽

2010 ◽

Vol 17 (1) ◽

pp. 147-157 ◽

Cited By ~ 6

Author(s):

Anna Konwisorz ◽

Anette Springwald ◽

Martina Haselberger ◽

Regina Goerse ◽

Olaf Ortmann ◽

...

Keyword(s):

Breast Cancer ◽

Ovarian Cancer ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Chromosome 1 ◽

Ovarian Cancer Cells ◽

General Role ◽

Estrogen Response ◽

Mcf 7

ICB-1 chromosome 1 open reading frame 38 (C1orf38) is a human gene initially described by our group to be involved in differentiation processes of cancer cells. Recently, we have reported ICB-1 as a novel estrogen target gene and identified an estrogen response element in its promoter. In this study, we examined the role of ICB-1 in regulation of proliferation of breast and ovarian cancer cells. We knocked down its expression in estrogen-dependent MCF-7 breast cancer cells and hormone-unresponsive SK-OV-3 ovarian cancer cells by stable transfection with a specific shRNA plasmid followed by G-418 selection. Knockdown of ICB-1 enabled a considerable estrogen response of SK-OV-3 cells in terms of proliferation. This transformation of SK-OV-3 cells into an estrogen-responsive phenotype was accompanied by upregulation of estrogen receptor α (ERα) expression and a significant decrease of ERβ expression on the mRNA level. Expression of ERα-dependent genes progesterone receptor, pS2, fibulin 1c, and c-fos was elevated in SK-OV-3 cells stably expressing ICB-1 shRNA. In MCF-7 cells, ICB-1 knockdown exerted similar effects on gene expression, supporting a general role of ICB-1 in estrogen responsiveness. Our data suggest that differentiation-associated gene ICB-1 might exert antagonistic actions on cellular estrogen response, which can result in inhibition of estradiol-triggered proliferation. The molecular mechanisms mediating this inhibitory effect of ICB-1 on estrogen signaling are suggested to be limitation of ERα transcript levels but sustaining high levels of ERβ, reducing both activation of ERα target genes and cellular proliferation. The identification of ICB-1 as a new player in endocrine-related cancer encourages further studies on the significance of this gene in cancer development and therapy.

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Anti-cancer effect of bee venom toxin and melittin in ovarian cancer cells through induction of death receptors and inhibition of JAK2/STAT3 pathway

Toxicology and Applied Pharmacology ◽

10.1016/j.taap.2011.10.009 ◽

2012 ◽

Vol 258 (1) ◽

pp. 72-81 ◽

Cited By ~ 121

Author(s):

Miran Jo ◽

Mi Hee Park ◽

Pushpa Saranya Kollipara ◽

Byeong Jun An ◽

Ho Sueb Song ◽

...

Keyword(s):

Ovarian Cancer ◽

Cancer Cells ◽

Death Receptors ◽

Bee Venom ◽

Ovarian Cancer Cells ◽

Stat3 Pathway ◽

Venom Toxin ◽

Anti Cancer

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Casein kinase I epsilon interacts with mitochondrial proteins for the growth and survival of human ovarian cancer cells

EMBO Molecular Medicine ◽

10.1002/emmm.201101094 ◽

2012 ◽

Vol 4 (9) ◽

pp. 952-963 ◽

Cited By ~ 35

Author(s):

Noah Rodriguez ◽

Junzheng Yang ◽

Kathleen Hasselblatt ◽

Shubai Liu ◽

Yilan Zhou ◽

...

Keyword(s):

Ovarian Cancer ◽

Cancer Cells ◽

Casein Kinase ◽

Mitochondrial Proteins ◽

Ovarian Cancer Cells ◽

Human Ovarian Cancer ◽

Casein Kinase I ◽

Growth And Survival ◽

Casein Kinase I Epsilon

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Anticancer effects of novel resveratrol analogues on human ovarian cancer cells

Molecular BioSystems ◽

10.1039/c7mb00128b ◽

2017 ◽

Vol 13 (6) ◽

pp. 1131-1141 ◽

Cited By ~ 9

Author(s):

Daniele Vergara ◽

Stefania De Domenico ◽

Andrea Tinelli ◽

Eleonora Stanca ◽

Loretta L. Del Mercato ◽

...

Keyword(s):

Ovarian Cancer ◽

Cancer Cells ◽

Molecular Mechanisms ◽

Ovarian Cancer Cells ◽

Human Ovarian Cancer ◽

Anticancer Effects ◽

Anti Cancer ◽

Resveratrol Analogues

We describe the molecular mechanisms of the action of novel trans-restricted analogues of resveratrol with enhanced anti-cancer properties.

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Heteroarotinoids with Anti-Cancer Activity Against Ovarian Cancer Cells

The Open Medicinal Chemistry Journal ◽

10.2174/1874104500701010011 ◽

2007 ◽

Vol 1 (1) ◽

pp. 11-23 ◽

Cited By ~ 10

Author(s):

Thanh C. Le ◽

K. Darrell Berlin ◽

Stacy D. Benson ◽

Margaret A. Eastman ◽

Gianna Bell-Eunice ◽

...

Keyword(s):

Ovarian Cancer ◽

Cancer Cells ◽

Ovarian Cancer Cells ◽

Anti Cancer ◽

Cancer Activity

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Heteroarotinoids with Anti-Cancer Activity Against Ovarian Cancer Cells

The Open Medicinal Chemistry Journal ◽

10.2174/187410450701011105 ◽

2007 ◽

Vol 1 (1) ◽

pp. 11-23

Author(s):

Thanh C. Le ◽

K. Darrell Berlin ◽

Stacy D. Benson ◽

Margaret A. Eastman ◽

Gianna Bell-Eunice ◽

...

Keyword(s):

Ovarian Cancer ◽

Cancer Cells ◽

Ovarian Cancer Cells ◽

Anti Cancer ◽

Cancer Activity

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RHAMM-Target Peptides Inhibit Proliferation and Viability of Cancer Cells

Biointerface Research in Applied Chemistry ◽

10.33263/briac114.1225212266 ◽

2021 ◽

Vol 11 (4) ◽

pp. 12252-12266

Keyword(s):

Ovarian Cancer ◽

Cancer Cells ◽

Ovarian Cancer Cells ◽

Targeted Cancer Therapy ◽

Proliferation Assay ◽

Cancer Breast ◽

Low Concentrations ◽

Skov3 Cells ◽

Anti Cancer ◽

The World

The incidence of cancer in the world is growing exponentially. Therefore, the search for targeted cancer therapy methods is the most urgent and actively developing the biomedicine field. This work is devoted to studying RHAMM-target peptides' effect on the proliferation and viability of ovarian cancer, prostate cancer, breast carcinoma, and adenocarcinoma of the breast duct cells. Cell proliferation was examined by a BrdU-based proliferation assay. Cell viability was assayed by the fluorescence method. It has been established that RHAMM-target peptides at a concentration of 2х10-7 M inhibited on ~ 55 % proliferation of MDA-MB-231 cells, on ~ 85 % proliferation of PC3m-LN4, and ~ 50 % proliferation of SKOV3 cells for 24 h. The results showed that the peptides inhibited the viability of ovarian cancer cells. In particular, peptide EEDFGEEAEEEA inhibited ovarian cancer cells' viability by 54%, peptide VEGEGEEGEEY by 63%, and peptide FTEAESNMNDLV by 57%. RHAMM-target peptides did not affect fibroblasts (non-tumor cells) and fibroblasts RHAMM(-/-). This work showed that RHAMM-target peptides at low concentrations of inhibited cancer cells' proliferation and viability. This effect was RHAMM mediated. RHAMM-target peptides are promising candidates for anti-cancer drugs.

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