scholarly journals Serum and follicular fluid levels of sirtuin 1, sirtuin 6, and resveratrol in women undergoing in vitro fertilization: an observational, clinical study

2018 ◽  
Vol 47 (2) ◽  
pp. 772-782 ◽  
Author(s):  
József Bódis ◽  
Endre Sulyok ◽  
Tamás Kőszegi ◽  
Krisztina Gödöny ◽  
Viktória Prémusz ◽  
...  
Keyword(s):  
Clinical Study ◽  
In Vitro Fertilization ◽  
Pregnant Women ◽  
Follicular Fluid ◽  
Clinical Pregnancy ◽  
Sirtuin 1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ovarian Hyperstimulation ◽  
Vitro Fertilization

Objective This observational, clinical study was designed to assess the role of sirtuin 1 (SIRT1), sirtuin 6 (SIRT6), and resveratrol in in vitro fertilization (IVF). Methods Paired serum and follicular fluid (FF) samples were obtained from 30 consecutive patients (age: 36.43 ± 4.17 years, body mass index: 22.90 ± 2.05 kg/m2, duration of infertility: 5.10 ± 2.80 years) who received IVF treatment. SIRT1, SIRT6, and resveratrol levels were measured by enzyme-linked immunosorbent assay. Results Ovarian hyperstimulation resulted in significantly higher serum SIRT1 levels in pregnant women (8 patients) compared with non-pregnant women (22 patients). SIRT6 levels remained unchanged after ovarian hyperstimulation, but were significantly lower in pregnant women compared with non-pregnant women before and after hyperstimulation. Both SIRTs were detected in FF, but they appeared to be independent of their serum levels. After correction for confounders, FF SIRT6 levels were positively related to mature oocytes (F = 6.609), whereas serum SIRT1 and SIRT6 levels were related to clinical pregnancy (F = 10.008, F = 5.268, respectively). Conclusions Our study shows that SIRT1 and SIRT6, but not resveratrol, are involved in human reproduction and they may have a role in oocyte maturation and clinical pregnancy in IVF.

scholarly journals Serum and follicular fluid levels of serotonin, kisspeptin, and brain-derived neurotrophic factor in patients undergoing in vitro fertilization: an observational study

2019 ◽  
Vol 48 (4) ◽  
pp. 030006051987933 ◽  
Author(s):  
József Bódis ◽  
Endre Sulyok ◽  
Tamás Kőszegi ◽  
Viktória Prémusz ◽  
Ákos Várnagy ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Neurotrophic Factor ◽  
Platelet Activating Factor ◽  
Reproductive Potential ◽  
Brain Derived Neurotrophic Factor ◽  
Secondary Outcome ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vitro Fertilization

Objective This study aimed to examine the effect of interactions between serotonin (5-HT), brain-derived neurotrophic factor (BDNF), and kisspeptin on the reproductive potential in women receiving in vitro fertilization (IVF). Methods Paired serum and follicular fluid (FF) samples were obtained from 30 consecutive patients receiving IVF. Primary and secondary outcome measures were the rate of chemical/clinical pregnancy and the number of mature oocytes and embryos, respectively. Serum and FF 5-HT, BDNF, kisspeptin, and platelet-activating factor (PAF) levels were measured by enzyme-linked immunosorbent assay. Results In response to ovarian hyperstimulation, serum 5-HT and kisspeptin levels significantly increased, whereas serum BDNF and PAF levels remained unchanged. These factors were detected in FF, but they were unrelated to serum levels. FF 5-HT and BDNF levels were positively correlated. Serum kisspeptin levels were negatively correlated with FF BDNF and serum and FF PAF levels. Women who were pregnant had significantly lower FF BDNF levels compared with women who were not pregnant (21.96±12.75 vs 47.63±52.90 µg/mL). Multivariate stepwise linear regression and logistic regression analyses showed that only 5-HT and kisspeptin improved IVF outcome. Conclusions This study indicates a role of serotoninergic mechanisms in success of IVF, but the contribution of interacting neuropeptides requires additional investigation.

scholarly journals Effect of endometriosis on the protein expression pattern of follicular fluid from patients submitted to controlled ovarian hyperstimulation for in vitro fertilization

2010 ◽  
Vol 25 (7) ◽  
pp. 1755-1766 ◽  
Author(s):  
Edson Guimaraes Lo Turco ◽  
Gustavo Henrique Martins Ferreira Souza ◽  
Jerusa Simone Garcia ◽  
Christina Ramires Ferreira ◽  
Marcos Nogueira Eberlin ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Protein Expression ◽  
Expression Pattern ◽  
Follicular Fluid ◽  
Controlled Ovarian Hyperstimulation ◽  
Ovarian Hyperstimulation ◽  
Protein Expression Pattern ◽  
Vitro Fertilization

Proteomic Analysis of Follicular Fluid from Patients with Endometriosis Submitted to Controlled Ovarian Hyperstimulation for In Vitro Fertilization.

2009 ◽  
Vol 81 (Suppl_1) ◽  
pp. 185-185
Author(s):  
Edson Guimaraes Lo Turco ◽  
Paula H. C. Lopes ◽  
Maisa M. S. Sumikawa ◽  
Thiesa Butterby Soler ◽  
Ricardo Pimenta Bertolla ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Proteomic Analysis ◽  
Controlled Ovarian Hyperstimulation ◽  
Ovarian Hyperstimulation ◽  
Vitro Fertilization

scholarly journals Follicular output rate tends to improve clinical pregnancy outcomes in patients with polycystic ovary syndrome undergoing in vitro fertilization-embryo transfer treatment

2019 ◽  
Vol 47 (10) ◽  
pp. 5146-5154
Author(s):  
Xiaoqing Tan ◽  
Yu Wen ◽  
Huixiao Chen ◽  
Ling Zhang ◽  
Bin Wang ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Clinical Outcomes ◽  
Embryo Transfer ◽  
Polycystic Ovary ◽  
Controlled Ovarian Hyperstimulation ◽  
Clinical Pregnancy ◽  
Ovarian Hyperstimulation ◽  
Output Rate ◽  
Vitro Fertilization

Objective This study aimed to examine the relationship between the follicular output rate (FORT) and clinical outcomes in patients with polycystic ovarian syndrome (PCOS). Methods A total of 841 patients with PCOS undergoing in vitro fertilization-embryo transfer (IVF-ET) were divided into three groups according to their FORT (low, middle, and high). Controlled ovarian hyperstimulation and clinical outcomes were compared retrospectively. Results Serum estradiol levels on the day of human chorionic gonadotropin (3780.5, 3599.9, and 3375.7 pg/mL) and the number of retrieved oocytes (17.5, 16.1, and 14.8) decreased from the high to low FORT groups. Pre-ovulatory follicle counts were significantly higher in the high FORT group than in the middle and low FORT groups. The number of retrieved oocytes, high-quality embryo rate, and clinical pregnancy rate decreased from the high to low FORT groups. The incidence of moderate and severe ovarian hyperstimulation syndrome (OHSS) in the middle FORT group was significantly lower than that in the high and low FORT groups. Conclusions FORT may be used to predict clinical outcomes of IVF/intracytoplasmic sperm injection-embryo transfer in patients with PCOS. Efforts should be made to prevent OHSS in patients with PCOS and a high or low FORT in controlled ovarian hyperstimulation cycles.

Immunoreactive inhibin in human follicular fluid in an ovarian hyperstimulation programme for in vitro fertilization: correlations and different forms

1993 ◽  
Vol 128 (5) ◽  
pp. 433-438 ◽  
Author(s):  
Bärbel Reichardt ◽  
Gerhard Mehltretter ◽  
Karin Lechner ◽  
Hans K Rjosk ◽  
Otto A Müller ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Cell Function ◽  
Human Menopausal Gonadotropin ◽  
Gel Chromatography ◽  
Ovarian Hyperstimulation ◽  
Human Follicular Fluid ◽  
Vitro Fertilization ◽  
Follicular Maturation

The inhibin concentration in 131 samples of human follicular fluid obtained from 31 women undergoing ovarian hyperstimulation for in vitro fertilization was measured using specific double antibody radioimmunoassay. We used the synthetic 1-32-α-inhibin as standard and radioiodinated 1-32-Tyr-α-inhibin as tracer. Antibodies were raised in rabbits by immunization with the synthetic peptide. Estradiol and progesterone concentrations were measured using commercial radioimmunoassays. Results: The inhibin concentration correlated with the estradiol (r = 0.57, N = 88.p<0.0001) and progesterone (r=0.82, N=88. p<0.0001) concentrations in human follicular fluid. The dosage of human menopausal gonadotropin given to individual patients correlated with the average inhibin concentration measured in their follicles (r = 0.72, N = 23, p<0.0001). Similarly, the size of follicles correlated with their inhibin content (r = 0.75, N = 131, p<0.0001). Nineteen samples of human follicular fluid originating from follicles of different size and volume were examined using gel-chromatography. In each human follicular fluid the main form of inhibin (32 kDa) was recovered. In small follicles (3 ml) we found 12.8±9.1% (mean±sd) of the whole immunoreactive inhibin eluating in the area of V0 (≤80 kDa). In the larger follicles (4-7 ml), however, only 4.4±4.2% of this large inhibin form could be found. Conclusions: Our data confirm that human menopausal gonadotropin stimulates ovarian inhibin production. In addition to the estradiol and progesterone concentrations, the inhibin concentration may be an index of granulosa cell function and follicular maturation. The occurrence of large molecular weight forms of inhibin in small follicles remains unclear. They may represent large precursor molecules which are proteolytically cleaved in more mature follicles.

scholarly journals CHARACTERISTIC OF OVARIAN AND ESTRADIOL CONCENTRATIONS IN THE FOLLICULAR FLUID OF SLAUGHTERED ACEH CATTLE

2020 ◽  
Vol 13 (4) ◽  
Author(s):  
Mulyadi Adam ◽  
Gholib Gholib ◽  
Hafizuddin Hafizuddin ◽  
Rumi Sahara Zamzami ◽  
Muhammad Bahi
Keyword(s):  
In Vitro Fertilization ◽  
Immunosorbent Assay ◽  
Follicular Fluid ◽  
Morphological Characteristic ◽  
In Vitro Maturation ◽  
Morphological Characteristics ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vitro Fertilization ◽  
Banda Aceh

The objective of this study was to characterize the morphology of ovary from slaughtered Aceh cow and measuring the concentration of estradiol in the follicular fluid to examine the potential utilizing for in-vitro fertilization (IVF) development in Aceh cattle. In total, 30 pairs of ovaries were collected from 30 Aceh cattle slaughtered at Banda Aceh abattoir. Morphological characteristics of ovaries observed were the size of ovaries, weight of ovaries, number of follicles, the diameter of follicles, corpus luteal (CL), and volume of follicular fluid. The follicular fluid was aspirated using a disposable syringe and analyzed the estradiol concentrations using enzyme-linked immunosorbent assay (ELISA). The follicles were grouped into 3 categories based on the diameter (Ø) of follicles: small follicles (Ø 1 - 5 mm), medium follicles (Ø 5 - 8 mm), and large follicles (Ø 8 mm). The morphological characteristic of ovaries varied between right and left ovaries in which right ovaries showed bigger size and weight compared to the left ovaries (P0.05), whereas the number of follicles and follicular fluid did not significantly different (P0.05). The concentrations of estradiol in follicular fluid of large and medium follicles were significantly higher compared to small follicles (P0.05), whereas the concentration of estradiol between large and medium follicles, did not significant different (P0.05). In conclusion, the follicles from abattoir can be used as potential oocytes resources to support IVF development for Aceh cattle in the future. The follicular fluid provided from these follicles can be an alternative for in vitro maturation media of oocytes due to containing high estradiol concentrations.

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