scholarly journals Serum and follicular fluid levels of serotonin, kisspeptin, and brain-derived neurotrophic factor in patients undergoing in vitro fertilization: an observational study

2019 ◽  
Vol 48 (4) ◽  
pp. 030006051987933 ◽  
Author(s):  
József Bódis ◽  
Endre Sulyok ◽  
Tamás Kőszegi ◽  
Viktória Prémusz ◽  
Ákos Várnagy ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Neurotrophic Factor ◽  
Platelet Activating Factor ◽  
Reproductive Potential ◽  
Brain Derived Neurotrophic Factor ◽  
Secondary Outcome ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vitro Fertilization

Objective This study aimed to examine the effect of interactions between serotonin (5-HT), brain-derived neurotrophic factor (BDNF), and kisspeptin on the reproductive potential in women receiving in vitro fertilization (IVF). Methods Paired serum and follicular fluid (FF) samples were obtained from 30 consecutive patients receiving IVF. Primary and secondary outcome measures were the rate of chemical/clinical pregnancy and the number of mature oocytes and embryos, respectively. Serum and FF 5-HT, BDNF, kisspeptin, and platelet-activating factor (PAF) levels were measured by enzyme-linked immunosorbent assay. Results In response to ovarian hyperstimulation, serum 5-HT and kisspeptin levels significantly increased, whereas serum BDNF and PAF levels remained unchanged. These factors were detected in FF, but they were unrelated to serum levels. FF 5-HT and BDNF levels were positively correlated. Serum kisspeptin levels were negatively correlated with FF BDNF and serum and FF PAF levels. Women who were pregnant had significantly lower FF BDNF levels compared with women who were not pregnant (21.96±12.75 vs 47.63±52.90 µg/mL). Multivariate stepwise linear regression and logistic regression analyses showed that only 5-HT and kisspeptin improved IVF outcome. Conclusions This study indicates a role of serotoninergic mechanisms in success of IVF, but the contribution of interacting neuropeptides requires additional investigation.

scholarly journals Serum and follicular fluid levels of sirtuin 1, sirtuin 6, and resveratrol in women undergoing in vitro fertilization: an observational, clinical study

2018 ◽  
Vol 47 (2) ◽  
pp. 772-782 ◽  
Author(s):  
József Bódis ◽  
Endre Sulyok ◽  
Tamás Kőszegi ◽  
Krisztina Gödöny ◽  
Viktória Prémusz ◽  
...  
Keyword(s):  
Clinical Study ◽  
In Vitro Fertilization ◽  
Pregnant Women ◽  
Follicular Fluid ◽  
Clinical Pregnancy ◽  
Sirtuin 1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ovarian Hyperstimulation ◽  
Vitro Fertilization

Objective This observational, clinical study was designed to assess the role of sirtuin 1 (SIRT1), sirtuin 6 (SIRT6), and resveratrol in in vitro fertilization (IVF). Methods Paired serum and follicular fluid (FF) samples were obtained from 30 consecutive patients (age: 36.43 ± 4.17 years, body mass index: 22.90 ± 2.05 kg/m2, duration of infertility: 5.10 ± 2.80 years) who received IVF treatment. SIRT1, SIRT6, and resveratrol levels were measured by enzyme-linked immunosorbent assay. Results Ovarian hyperstimulation resulted in significantly higher serum SIRT1 levels in pregnant women (8 patients) compared with non-pregnant women (22 patients). SIRT6 levels remained unchanged after ovarian hyperstimulation, but were significantly lower in pregnant women compared with non-pregnant women before and after hyperstimulation. Both SIRTs were detected in FF, but they appeared to be independent of their serum levels. After correction for confounders, FF SIRT6 levels were positively related to mature oocytes (F = 6.609), whereas serum SIRT1 and SIRT6 levels were related to clinical pregnancy (F = 10.008, F = 5.268, respectively). Conclusions Our study shows that SIRT1 and SIRT6, but not resveratrol, are involved in human reproduction and they may have a role in oocyte maturation and clinical pregnancy in IVF.

scholarly journals CHARACTERISTIC OF OVARIAN AND ESTRADIOL CONCENTRATIONS IN THE FOLLICULAR FLUID OF SLAUGHTERED ACEH CATTLE

2020 ◽  
Vol 13 (4) ◽  
Author(s):  
Mulyadi Adam ◽  
Gholib Gholib ◽  
Hafizuddin Hafizuddin ◽  
Rumi Sahara Zamzami ◽  
Muhammad Bahi
Keyword(s):  
In Vitro Fertilization ◽  
Immunosorbent Assay ◽  
Follicular Fluid ◽  
Morphological Characteristic ◽  
In Vitro Maturation ◽  
Morphological Characteristics ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vitro Fertilization ◽  
Banda Aceh

The objective of this study was to characterize the morphology of ovary from slaughtered Aceh cow and measuring the concentration of estradiol in the follicular fluid to examine the potential utilizing for in-vitro fertilization (IVF) development in Aceh cattle. In total, 30 pairs of ovaries were collected from 30 Aceh cattle slaughtered at Banda Aceh abattoir. Morphological characteristics of ovaries observed were the size of ovaries, weight of ovaries, number of follicles, the diameter of follicles, corpus luteal (CL), and volume of follicular fluid. The follicular fluid was aspirated using a disposable syringe and analyzed the estradiol concentrations using enzyme-linked immunosorbent assay (ELISA). The follicles were grouped into 3 categories based on the diameter (Ø) of follicles: small follicles (Ø 1 - 5 mm), medium follicles (Ø 5 - 8 mm), and large follicles (Ø 8 mm). The morphological characteristic of ovaries varied between right and left ovaries in which right ovaries showed bigger size and weight compared to the left ovaries (P0.05), whereas the number of follicles and follicular fluid did not significantly different (P0.05). The concentrations of estradiol in follicular fluid of large and medium follicles were significantly higher compared to small follicles (P0.05), whereas the concentration of estradiol between large and medium follicles, did not significant different (P0.05). In conclusion, the follicles from abattoir can be used as potential oocytes resources to support IVF development for Aceh cattle in the future. The follicular fluid provided from these follicles can be an alternative for in vitro maturation media of oocytes due to containing high estradiol concentrations.

Apoptosis and proliferation factors in serum and follicular fluid from women undergoing in vitro fertilization

2003 ◽  
Vol 79 (3) ◽  
pp. 634-636 ◽  
Author(s):  
Angeliki Sarandakou ◽  
Ariadne Malamitsi-Puchner ◽  
Stavroula Baka ◽  
Demetrios Rizos ◽  
Dimitrios Hassiakos ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Vitro Fertilization
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