Effects of a Single Dose of Rabbit Anti-mouse Lymphocyte Serum on Tumors Induced in Mice by 7,12-Dimethylbenz (a) anthracene Given at Birth

1972 ◽  
Vol 58 (6) ◽  
pp. 397-408 ◽  
Author(s):  
Carlo D. Baroni ◽  
Pietro Mingazzini ◽  
Paolo Pesando ◽  
Anna Cavallero ◽  
Stefania Uccini ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Single Dose ◽  
Malignant Lymphoma ◽  
Single Injection ◽  
Latency Period ◽  
Skin Tumors ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Malignant Lymphomas ◽  
Mouse Lymphocyte

The effects of ALS (anti-lymphocyte serum) and NRS (normal rabbit serum) treatments on the development of malignant lymphoma, lung, subcutaneous and skin tumors induced in mice by 7,12-dimethylbenz (a) anthracene (DMBA) are described. Groups of Charles-River mice, injected at birth with a dose of 100 μg of DMBA, received a single injection of ALS or NRS at the same time as DMBA administration or 10, 20, 30, 40 and 50 days after birth. Incidence, latency, histology and spread of the tumors were studied in all groups. It was found that both ALS and NRS increased tumor incidence and shortened their latency period. Malignant lymphomas were the main tumors whose latency was shortened either by ALS or NRS treatment. In addition ALS treatment apparently increased dissemination of DMBA induced lymphoma in bone marrow.

Effects of Repeated Injections of Rabbit Anti-mouse Lymphocyte Serum on Tumors Induced in Mice by 7,12-dimethylbenz (a) anthracene Given at Birth.

1973 ◽  
Vol 59 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Carlo D. Baroni ◽  
Roberto Scelsi ◽  
Laura Peronace ◽  
Stefania Uccini ◽  
Anna Cavallero
Keyword(s):  
Latency Period ◽  
Chronic Administration ◽  
Repeated Administration ◽  
The Other ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Mean Survival Time ◽  
Mouse Lymphocyte ◽  
Tumor Bearing ◽  
The Mean

The present paper describes the effects of repeated administration of rabbit anti-mouse lymphocyte serum (ALS) or normal rabbit serum (NRS) on tumors induced in Charles–River mice by 7,12-dimethylbenz (a) anthracene (DMBA) given at birth. ALS or NRS were given at the same time of DMBA administration and subsequently at weekly intervals for the first 10 weeks of life or at daily intervals for 7 days during the first, second, third or fourth week of life. Incidence, latency, diffusion and histology of the tumors were studied. It was found that either chronic administration of ALS or treatment of very young mice with the serum, greatly reduced the mean survival time of mice, markedly increased the number of tumor bearing mice and the incidence of all histological types of tumors, and decreased their latency period. Administration of ALS in the other experimental groups gave results essentially similar to those observed in DMBA control and NRS treated mice.

Effects of anti-thymocyte serum on the eosinophil and lysophospholipase responses in mice infected withTrichinella spiralis

Parasitology ◽  
1987 ◽  
Vol 94 (1) ◽  
pp. 115-122 ◽  
Author(s):  
K. Adewusi ◽  
A. J. Goven
Keyword(s):  
Bone Marrow ◽  
Experimental Animal ◽  
Peripheral Blood ◽  
Trichinella Spiralis ◽  
Peripheral Blood Lymphocytes ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Significant Suppression ◽  
Thymus Cell ◽  
Lysophospholipase Activity

SUMMARYAnti-thymocyte serum (ATS) was administered to CD-1 mice infected with 100Trichinella spiralislarvae and its effects on intestinal lysophospholipase (EC 3.1.1.5) activity, peripheral blood lymphocytes and bone marrow, peripheral blood and intestinal eosinophilia were assayed in the same experimental animal. The ATS caused a significant suppression of both the tissue lysophospholipase response and eosinophilia, in all three compartments, when compared to the values found in infected mice that were either treated with normal rabbit serum (NRS) or untreated. The suppressed eosinophil response and reduced lysophospholipase activity demonstrated a close temporal relation throughout the experiment. These findings support the hypothesis that helminth parasite-induced eosinophilia is the cause of increased lysophospholipase activity present in parasitized tissue and that the responses are thymus cell dependent.

Partially matched bone marrow transplantation in patients with myelodysplastic syndromes.

1988 ◽  
Vol 6 (12) ◽  
pp. 1851-1855 ◽  
Author(s):  
N J Bunin ◽  
J T Casper ◽  
C Chitambar ◽  
J Hunter ◽  
R Truitt ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Marrow Transplantation ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Trisomy 8 ◽  
Monosomy 7 ◽  
Patients Âgés ◽  
Curative Therapy ◽  
Unrelated Donors

Six patients with a myelodysplastic syndrome (MDS) were treated with bone marrow transplantation (BMT) using partially-matched related (3) or unrelated (3) donors. Patients' ages ranged from 7 to 31 years (median, 10 years). Bone marrow karyotype abnormalities were present in five patients included four with monosomy 7 and one with trisomy 8. One patient was in complete remission before transplant; the remaining five had excess of blasts or were undergoing leukemic transformation. Donor, and recipient were mismatched at the DR locus (2), A locus (2), B locus (1), or A and B loci (1). Conditioning included busulfan, cytarabine, cyclophosphamide, methylprednisolone, and total body irradiation. Cyclosporine was started on day -1. Marrows were T-cell depleted using a monoclonal antibody (MoAb) (CD3) and normal rabbit serum. Four patients engrafted routinely. One patient died of aspergillosis before engraftment (day 12) and one patient failed to engraft on first attempt, but engrafted following additional preparation. Median time to neutrophils greater than 500/microL and platelets greater than 25,000/microL were 16 and 19 days, respectively. Acute graft-v-host disease (GVHD) was less than or equal to grade II in all patients. One patient died with recurrent disease (day 257). One patient died at day 515 of pancreatitis and respiratory failure. Three patients are alive and disease-free at 240, 395, and 560 days post-BMT including two patients with unrelated donors. Partially matched T-depleted bone marrow from related or unrelated donors may be effective, and possibly curative therapy for patients with MDS who lack a histocompatibility locus antigen (HLA)-identical sibling donor.

scholarly journals Analysis of human myelogenous leukemia cells in the fluorescence- activated cell sorter using a tumor-specific antiserum

Blood ◽  
1983 ◽  
Vol 61 (5) ◽  
pp. 858-866 ◽  
Author(s):  
AJ Malcolm ◽  
PM Logan ◽  
RC Shipman ◽  
R Kurth ◽  
JG Levy
Keyword(s):  
Bone Marrow ◽  
Acute Myelogenous Leukemia ◽  
Rabbit Antiserum ◽  
Lymphocytic Leukemia ◽  
Myelogenous Leukemia ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Cell Sorter ◽  
Chronic Granulocytic Leukemia ◽  
Acute Myelogenous

Abstract The properties of a rabbit antiserum (anti-AML) raised to a purified protein from membranes of human acute myelogenous leukemia (AML) cells is described. Bone marrow and peripheral blood leukocytes (PBL) from either normal individuals or patients with either myeloproliferative or other disorders were analyzed in a fluorescence-activated cell sorter (FACS IV) after labeling with anti-AML, normal rabbit serum (NRS), or antiserum raised to normal human membrane antigens. Of 40 cell samples from patients with acute myelogenous leukemia, 39 reacted strongly with the anti-AML antiserum. Similarly, all of 19 specimens from patients with chronic granulocytic leukemia reacted with the anti-AML. When 42 bone marrow or PBL samples from patients with a variety of lymphoproliferative disorders were examined, 2 specimens reacted with the antiserum, both from individuals with diagnoses of acute lymphocytic leukemia (ALL). None of the 14 normal bone marrow or PBL donor specimens tested reacted with the antiserum. It was also found that essentially all samples from patients in clinical remission from AML had high numbers of cells reactive with the anti-AML. When cells from such individuals were labeled and sorted on the FACS IV, it was found that cells fluorescing strongly with the anti-AML contained cells of both myeloid and lymphoid origin. The implications of these results are discussed.

scholarly journals An Evaluation of Paraffin Sections of Aspirated Bone Marrow in Malignant Lymphomas

Blood ◽  
1955 ◽  
Vol 10 (8) ◽  
pp. 820-830 ◽  
Author(s):  
JACQUELINE D. PETTET ◽  
GERTRUDE L. PEASE ◽  
TALBERT COOPER
Keyword(s):  
Bone Marrow ◽  
Malignant Lymphoma ◽  
Peripheral Blood ◽  
Hodgkin's Disease ◽  
Hodgkin’S Disease ◽  
Reticulum Cell ◽  
Reticulum Cell Sarcoma ◽  
Paraffin Sections ◽  
Malignant Lymphomas ◽  
Cell Sarcoma

Abstract Examination of serial paraffin sections of bone-marrow aspirations has increased the known incidence of marrow involvement in malignant lymphomas. The results of this study can be seen in the table. In the 72 cases reviewed here, lesions compatible with malignant lymphoma were seen in paraffin sections in 22 cases. In 12 cases this was the only material available for diagnosis at the time. In an additional seven cases paraffin sections were considered suggestive of malignant lymphoma and in five of these a diagnosis could be made on the basis of examination of both smear and paraffin section. This procedure is particularly helpful in Hodgkin’s disease and reticulum cell sarcoma, where marrow smears are only rarely diagnostic. In general, the patients in whom lesions were found in the bone marrow tended to have more advanced disease than those in whom lesions were not found, and abnormalities in the peripheral blood were more common. This was especially true of patients with Hodgkin’s disease, less so in those with reticulum cell sarcoma. Patients with lymphosarcoma, lymphocytic type, did not show this difference, either in the extent of the disease or in findings on examination of peripheral blood. Diagnosis was most difficult in patients who had lymphocytic lymphosarcoma, since it was occasionally difficult to distinguish between the benign aggregations of lymphocytes which are not infrequently found in a large number of unrelated conditions and those aggregations which represented malignancy. Differentiation was made on the basis of number of aggregations, degree of circumscription, presence or absence of reaction centers and the cytologic appearance of the cells. Examination of the smears of the marrow is most helpful in these cases. Seven patients in this series (9.7 per cent) had granulomatous lesions in the marrow. In five of these the final diagnosis was Hodgkin’s disease, in one reticulum cell sarcoma and in one follicular lymphoma. Although these lesions were not specific, the incidence is high enough to make the finding of a granuloma of some significance in any patient suspected of having malignant lymphoma. The importance of thorough examination of any material obtained in an apparent dry tap and the value of repeated marrow-examinations is emphasized by three cases in this series. Since in many cases diagnostic lesions are found in only one portion of the material, examination of only one section of the paraffin sections of aspirated bone marrow is not sufficient for proper evaluation. This fact is emphasized by the greater incidence of positive findings in this report than in that of Cooper and Watkins. Studies of bone marrow should be particularly helpful in instances in which the diagnosis of malignant lymphoma is suggested by clinical features but cannot be proved by biopsy of peripheral nodes or other readily accessible tissue.

scholarly journals Analysis of human myelogenous leukemia cells in the fluorescence- activated cell sorter using a tumor-specific antiserum

Blood ◽  
1983 ◽  
Vol 61 (5) ◽  
pp. 858-866
Author(s):  
AJ Malcolm ◽  
PM Logan ◽  
RC Shipman ◽  
R Kurth ◽  
JG Levy
Keyword(s):  
Bone Marrow ◽  
Acute Myelogenous Leukemia ◽  
Rabbit Antiserum ◽  
Lymphocytic Leukemia ◽  
Myelogenous Leukemia ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Cell Sorter ◽  
Chronic Granulocytic Leukemia ◽  
Acute Myelogenous

The properties of a rabbit antiserum (anti-AML) raised to a purified protein from membranes of human acute myelogenous leukemia (AML) cells is described. Bone marrow and peripheral blood leukocytes (PBL) from either normal individuals or patients with either myeloproliferative or other disorders were analyzed in a fluorescence-activated cell sorter (FACS IV) after labeling with anti-AML, normal rabbit serum (NRS), or antiserum raised to normal human membrane antigens. Of 40 cell samples from patients with acute myelogenous leukemia, 39 reacted strongly with the anti-AML antiserum. Similarly, all of 19 specimens from patients with chronic granulocytic leukemia reacted with the anti-AML. When 42 bone marrow or PBL samples from patients with a variety of lymphoproliferative disorders were examined, 2 specimens reacted with the antiserum, both from individuals with diagnoses of acute lymphocytic leukemia (ALL). None of the 14 normal bone marrow or PBL donor specimens tested reacted with the antiserum. It was also found that essentially all samples from patients in clinical remission from AML had high numbers of cells reactive with the anti-AML. When cells from such individuals were labeled and sorted on the FACS IV, it was found that cells fluorescing strongly with the anti-AML contained cells of both myeloid and lymphoid origin. The implications of these results are discussed.

Identification of Maize Rayado Fino Virus by Immunoelectron Microscopy

1985 ◽  
Vol 43 ◽  
pp. 636-637
Author(s):  
O. E. Bradfute
Keyword(s):  
Electron Microscopy ◽  
Zea Mays ◽  
Costa Rica ◽  
Severe Disease ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Virus Particles ◽  
Far North ◽  
Maize Rayado Fino Virus ◽  
Antibody Coating

Maize rayado fino virus (MRFV) causes a severe disease of corn (Zea mays) in many locations throughout the neotropics and as far north as southern U.S. MRFV particles detected by direct electron microscopy of negatively stained sap from infected leaves are not necessarily distinguishable from many other small isometric viruses infecting plants (Fig. 1).Immunosorbent trapping of virus particles on antibody-coated grids and the antibody coating or decoration of trapped virus particles, was used to confirm the identification of MRFV. Antiserum to MRFV was supplied by R. Gamez (Centro de Investigacion en Biologia Celular y Molecular, Universidad de Costa Rica, Ciudad Universitaria, Costa Rica).Virus particles, appearing as a continuous lawn, were trapped on grids coated with MRFV antiserum (Fig. 2-4). In contrast, virus particles were infrequently found on grids not exposed to antiserum or grids coated with normal rabbit serum (similar to Fig. 1). In Fig. 3, the appearance of the virus particles (isometric morphology, 30 nm diameter, stain penetration of some particles, and morphological subunits in other particles) is characteristic of negatively stained MRFV particles. Decoration or coating of these particles with MRFV antiserum confirms their identification as MRFV (Fig. 4).

STUDIES ON THE MECHANISM OF DECIDUALIZATION. I.

1963 ◽  
Vol 42 (2) ◽  
pp. 225-232 ◽  
Author(s):  
M. C. Shelesnyak ◽  
Peretz F. Kraicer ◽  
Gerard H. Zeilmaker
Keyword(s):  
Single Dose ◽  
Single Injection

ABSTRACT The concept of an oestrogen surge as a prerequisite for successful decidualization of the progravid uterus and consequently nidation in the rat was tested. Experiments were designed to see whether administration of an oestrogen antagonist at specific times would block decidualization (and nidation); whether the ovary was the source of the oestrogen, and whether a single dose of oestrogen, in the absence of the ovary (if the ovary is the source) would allow the uterus to respond to a decidualizing stimulus. It was found that when given prior to the surge, the oestrogen antagonist prevented decidualization and interfered with nidation. The ovary is thus the source of the oestrogen; and a single injection of oestradiol can act as a substitute for the surge.

Localization of oxytocin and neurophysin in baboon (Papio anubis) corpus luteum by immunocytochemistry

1986 ◽  
Vol 113 (4) ◽  
pp. 570-575 ◽  
Author(s):  
Firyal S. Khan-Dawood
Keyword(s):  
Corpus Luteum ◽  
Rabbit Serum ◽  
Corpora Lutea ◽  
Positive Staining ◽  
Normal Rabbit Serum ◽  
Fallopian Tubes ◽  
Papio Anubis ◽  
Luteal Cells ◽  
Luteal Tissue ◽  
Immunocytochemical Reaction

Abstract. Immunoreactive oxytocin is detectable in the corpora lutea of women and cynomolgus monkeys by radioimmunoassay. To localize the presence of oxytocin and neurophysin I in ovarian tissues of subhuman primates, three corpora lutea and ovarian stromal tissues and two Fallopian tubes obtained during the menstrual cycle of the baboon and decidua from two pregnant baboons were examined using highly specific antisera against either oxytocin or neurophysin I and preoxidase-antiperoxidase light microscopy immunohistochemistry. Oxytocin-like as well as neurophysin I-like immunoreactivities were found in some cells of all the corpora lutea only, but could not be demonstrated in ovarian stromal tissues, Fallopian tubes and decidua. Specificity of the immunocytochemical reaction was further confirmed by immunoabsorption of the antiserum with excess oxytocin or neurophysin, after which the immunoreactivities for both oxytocin and neurophysin in the luteal tissue were negative. Similar controls using normal rabbit serum gave no positive staining for either oxytocin or neurophysin. Counterstaining of the positive immunoreactivities for oxytocin and neurophysin I with Mayer's haematoxylin and eosin demonstrated clearly that the oxytocin and neurophysin I appeared as granular material mainly within the cytoplasm of the luteal cells. The localization of immunoreactive oxytocin and neurophysin I in the corpus luteum of the baboon demonstrates directly the presence of these two neurohypophysial peptides within primate luteal cells and suggests their local production.

scholarly journals Increased cytotoxicity of normal rabbit serum for lectin-resistant mutants of animal cells.

1984 ◽  
Vol 160 (4) ◽  
pp. 1241-1246
Author(s):  
C Jones
Keyword(s):  
Structural Changes ◽  
Chinese Hamster ◽  
Rabbit Serum ◽  
Cytotoxic Action ◽  
Normal Rabbit Serum ◽  
Animal Cells ◽  
Naturally Occurring ◽  
Alternate Complement Pathway ◽  
Surface Carbohydrates ◽  
Naturally Occurring Antibodies

Plant lectins are cytotoxic and can be used to select for mutants of animal cells that exhibit structural changes in cell surface carbohydrates reflecting glycosylation defects. We isolated eight lectin mutants of Chinese hamster ovary (CHO) cells that appear to represent three different phenotype classes. These lectin mutants were much more sensitive to the cytotoxic action of normal rabbit serum (NRS) than were the parental cells. This increased cytotoxicity was heat sensitive, specifically absorbed, and inhibited by simple and complex carbohydrates. No killing was observed under conditions in which only the alternate complement pathway was active. An NRS-resistant subclone that was isolated from one lectin mutant was shown to have also regained wild type behavior when tested with the lectins. The possibility that naturally occurring antibodies in rabbit serum are reacting with incomplete carbohydrate chains on the surface of the lectin mutants is discussed.

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