Adsorption of Lipoprotein Containing Apolipoprotein-B through Plasma Separation for Treatment of Familial Hypercholesterolemia

1986 ◽  
Vol 9 (5) ◽  
pp. 343-348 ◽  
Author(s):  
M. Odaka ◽  
H. Kobayashi ◽  
K. Soeda ◽  
N. Murotani ◽  
Y. Saito ◽  
...  
Keyword(s):  
Total Cholesterol ◽  
Adsorption Capacity ◽  
Familial Hypercholesterolemia ◽  
Apolipoprotein B ◽  
Clinical Results ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Affinity Adsorbent ◽  
Excellent Selectivity

For the treatment of familial hypercholesterolemia, Liposorber LA-40 was clinically applied. The Liposorber is a commercially developed affinity adsorbent for plasma perfusion which selectivily adsorbs low density lipoproteins and very low density lipoproteins and is specially designed for plasmapheretic treatment of hypercholesterolemia. The Liposorber column, containing activated cellulose beads having an affinity for liporpotein containing apolipoprotein-B, has an excellent adsorption capacity, excellent selectivity, minimum albumin loss. This new apheresis system was applied to 2 clinical cases. After seven months of trial perfusion every 2 weeks, patient condition was good, with a level of total cholesterol under 300 mg/dl. No replacement fluids were given during or after treatment. In this paper, clinical results of these patients were shown and the mechanism of adsorption of this specific adsorbent was discussed.

scholarly journals The Assembly and Secretion of Apolipoprotein B-48-containing Very Low Density Lipoproteins in McA-RH7777 Cells

2000 ◽  
Vol 275 (14) ◽  
pp. 10506-10513 ◽  
Author(s):  
Pia Stillemark ◽  
Jan Borén ◽  
Maria Andersson ◽  
Thomas Larsson ◽  
Sabina Rustaeus ◽  
...  
Keyword(s):  
Apolipoprotein B ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins

scholarly journals Palmitoylation of Apolipoprotein B Is Required for Proper Intracellular Sorting and Transport of Cholesteroyl Esters and Triglycerides

2000 ◽  
Vol 11 (2) ◽  
pp. 721-734 ◽  
Author(s):  
Yang Zhao ◽  
James B. McCabe ◽  
Jean Vance ◽  
Luc G. Berthiaume
Keyword(s):  
Apolipoprotein B ◽  
Neutral Lipids ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Hydrophobic Core ◽  
Wild Type ◽  
Very Low Density Lipoproteins ◽  
Structural Requirement ◽  
Lipoprotein Particles ◽  
Intracellular Sorting

Apolipoprotein B (apoB) is an essential component of chylomicrons, very low density lipoproteins, and low density lipoproteins. ApoB is a palmitoylated protein. To investigate the role of palmitoylation in lipoprotein function, a palmitoylation site was mapped to Cys-1085 and removed by mutagenesis. Secreted lipoprotein particles formed by nonpalmitoylated apoB were smaller and denser and failed to assemble a proper hydrophobic core. Indeed, the relative concentrations of nonpolar lipids were three to four times lower in lipoprotein particles containing mutant apoB compared with those containing wild-type apoB, whereas levels of polar lipids isolated from wild-type or mutant apoB lipoprotein particles appeared identical. Palmitoylation localized apoB to large vesicular structures corresponding to a subcompartment of the endoplasmic reticulum, where addition of neutral lipids was postulated to occur. In contrast, nonpalmitoylated apoB was concentrated in a dense perinuclear area corresponding to the Golgi compartment. The involvement of palmitoylation as a structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle and its intracellular sorting represent novel roles for this posttranslational modification.

Nephelometry of apolipoprotein B in human serum.

1979 ◽  
Vol 25 (2) ◽  
pp. 221-226 ◽  
Author(s):  
C C Heuck ◽  
G Schlierf
Keyword(s):  
Light Scattering ◽  
Apolipoprotein B ◽  
Neutral Lipids ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Serum Samples ◽  
Very Low Density Lipoproteins ◽  
Ionic Detergent ◽  
Kinetics Of

Abstract We studied the development of light scattering in the reaction between anti-apolipoprotein B and apolipoprotein B in intact very-low-density lipoproteins (I) and low-density lipoproteins (II) as well as in lipoproteins treated with lipases, and found considerable differences in the kinetics of the immunoreaction for the two lipoprotein classes. Pre-incubation with triglyceride lipase and cholesterol esterase caused a decrease of final light scattering in I but only minimal changes in the reaction with II. Non-ionic detergent not only decreased the original light scattering in hyperlipemic serum samples, but also accelerated the immunoreaction. Under standardized conditions, results of quantitative nephelometry correlated highly significantly with quantitative determination of apolipoprotein B by radial immunodiffusion, both for normolipemic and hyperlipoproteinemic serum samples. The nonspecific light scattering caused by neutral lipids in intact lipoproteins could be minimized when samples were pre-incubated with lipolytic enzymes.

scholarly journals The Diabetes Prevention Program: A Worksite Experience

AAOHN Journal ◽  
2005 ◽  
Vol 53 (11) ◽  
pp. 499-505 ◽  
Author(s):  
Steven G. Aldana ◽  
Marilyn Barlow ◽  
Rebecca Smith ◽  
Frank G. Yanowitz ◽  
Ted Adams ◽  
...  
Keyword(s):  
Glucose Tolerance ◽  
Total Cholesterol ◽  
Prevention Program ◽  
Diabetes Prevention ◽  
Diabetes Prevention Program ◽  
Low Density Lipoproteins ◽  
High Density Lipoproteins ◽  
Oral Glucose ◽  
Low Density ◽  
Very Low Density Lipoproteins

The purpose of this study was to determine if the U.S. National Institutes of Health Diabetes Prevention Program (DPP) could be successfully implemented in a worksite setting. Thirty-seven adult employees of BD Medical Systems of Sandy, Utah were enrolled in a single-group time-series study using the DPP. Two-hour oral glucose tolerance tests (OGTT) and other outcomes were measured at baseline, 6 months, and 12 months. Weight, body mass index, waist circumference, 2-hour OGTT, very low density lipoproteins, triglycerides, and aerobic fitness were significantly improved at 6 and 12 months and showed overall significant improvement across time. Fasting blood insulin, total cholesterol, low density lipoproteins, and total cholesterol/high density lipoproteins ratio were significantly improved at 6 months, but not at 12 months. Eighteen of the program participants (51%) were no longer in the pre-diabetes and diabetes categories after 1 year. Existing worksite health promotion and occupational health professionals can successfully offer the DPP and help employees improve glucose tolerance.

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