scholarly journals Genetic Variability among Blastoschizomyces Capitatus Isolates from Different Clinical Sources

2005 ◽  
Vol 18 (3) ◽  
pp. 531-539 ◽  
Author(s):  
E. Pontieri ◽  
C. Caracciolo ◽  
T. Ceddia ◽  
B. Oliva ◽  
A. Ferrini ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Restriction Enzymes ◽  
Restriction Endonuclease Analysis ◽  
Electrophoretic Karyotype ◽  
Genetic Groups ◽  
Group I ◽  
Blastoschizomyces Capitatus ◽  
Reference Strains ◽  
Endonuclease Analysis

Sixteen clinical isolates and nine ATCC reference strains of Blastoschizomyces capitatus were analysed genetically, examined for the cellobiose, arbutin and salicin assimilation and tested for the aspartyl-proteinase secretion. The restriction endonuclease analysis (REA) with HpaII and HinfI enzymes and the electrophoretic karyotype (EK) were investigated. Both the restriction enzymes revealed two groups (I, II) constituted by the same isolates: 17 isolates (68%) in group I and 8 (32%) in group II. The EK analysis revealed sixteen groups. These data prompts for a genetic variability of the isolates of Blastoschizomyces capitatus and their account in two distinct genetic groups as suggested by REA. This grouping was confirmed by examing the utilisation of cellobiose, arbutin and salicin. The tests for secretory aspartyl proteinase (Sap) were positive only for three isolates, suggesting a marginal role of this specific enzyme in pathogenesis for these isolates.

scholarly journals Geographic distribution of restriction types ofMycobacterium bovisisolates from brush-tailed possums (Trichosurus vulpecula) in New Zealand

1986 ◽  
Vol 96 (3) ◽  
pp. 431-438 ◽  
Author(s):  
D. M. Collins ◽  
G. W. De Lisle ◽  
D. M. Gabric
Keyword(s):  
New Zealand ◽  
High Resolution ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Restriction Enzymes ◽  
Restriction Endonuclease Analysis ◽  
Trichosurus Vulpecula ◽  
Typing System ◽  
Dna Restriction ◽  
Endonuclease Analysis

SUMMARYDNA restriction endonuclease analysis was used for intra-specific typing ofMycobacterium bovisisolates from 83 brush-tailed possums (Trichosurus vulpecula) obtained between 1982 and 1984 from the three major regions in New Zealand with endemic bovine tuberculosis. All the isolates were found to be genetically very similar. Differentiation of the isolates into 33 restriction types was achieved by using high-resolution electrophoresis and the combined results from separate digestions with the restriction enzymesBstEII,PvuII andBclI. The typing system was entirely reproducible. Isolates of the same type were usually found in adjacent localities and were always limited to one of the three major regions. In some cases, isolates of the same type were found in both 1982 and 1984. The phenotypic significance of the small genetic differences identified between different isolates is unknown. The typing system will be useful for monitoring the transmission ofM. bovisto other species and the future spread of differentM. bovistypes through possum populations.

Copper minerals from volcanic exhalations – a unique family of natural compounds: crystal-chemical review

2018 ◽  
Vol 74 (6) ◽  
pp. 502-518 ◽  
Author(s):  
Igor V. Pekov ◽  
Natalia V. Zubkova ◽  
Dmitry Yu. Pushcharovsky
Keyword(s):  
High Temperature ◽  
Crystal Chemistry ◽  
Chemical Characterization ◽  
Crystal Chemical ◽  
Alkali Cations ◽  
Genetic Groups ◽  
Copper Mineralization ◽  
Group I ◽  
Group Ii

The crystal-chemical characterization of oxysalts (sulfates, arsenates, vanadates, selenites, silicates, molybdates and borates), chlorides and oxides with species-defining Cu2+ formed in volcanic fumaroles (96 minerals representing 80 structure types; 81 species are endemic to fumarolic formation) is given. Copper minerals are known only from oxidizing-type fumaroles. The most diverse copper mineralization occurs at the Tolbachik volcano (Kamchatka, Russia). Copper minerals from fumarolic systems are subdivided into two genetic groups: Group I are minerals formed in the hot zones of fumaroles (>473 K, mainly 673–973 K) and Group II are minerals formed in the moderately hot zones of fumaroles (<473 K, mainly at 343–423 K). Group I includes 81 mineral species. Their most defining chemical feature is that all of them are hydrogen-free, and many of them contain the additional anion O2−. In comparison with minerals from other geological environments, in minerals of Group I the Cu2+ cation exhibits the strongest affinity for four- and fivefold coordinations and the strongest distortion of Cu2+-centred octahedra. Group II consists of 15 chlorides and sulfates including 13 H-bearing species. In these minerals the Cu2+ cation shows affinity for octahedral coordination, with OH− and/or H2O0 as ligands. In terms of crystal chemistry these minerals are closer to supergene minerals rather than to high-temperature fumarolic species. Temperature is the major factor governing the crystal chemistry of Cu2+ oxysalts and chlorides in low-pressure systems. The defining feature of fumarolic copper mineralization over this whole temperature range is the important role of alkali cations. The available data on complexes of Cu2+-centred polyhedra in the structures of natural oxysalts and halides are summarized and reviewed. Isomorphism in copper minerals from volcanic exhalations is discussed. The structures of high-temperature Cu oxysalts with additional O2− anions (i.e. O atoms non-bonded to S6+, Mo6+, As5+, V5+, Se4+ or B3+) are also interpreted using an approach based on oxocentred tetrahedra.

scholarly journals Mitochondrial DNA differentiation of Japanese diploid and triploid Paragonimus westermani

1994 ◽  
Vol 68 (1) ◽  
pp. 7-11 ◽  
Author(s):  
T. Agatsuma ◽  
L. Yang ◽  
D. Kim ◽  
H. Yonekawa
Keyword(s):  
Mitochondrial Dna ◽  
Restriction Endonuclease ◽  
Restriction Enzymes ◽  
Restriction Endonuclease Analysis ◽  
Paragonimus Westermani ◽  
Endonuclease Analysis

AbstractRestriction endonuclease analysis of mitochondrial DNA was carried out in order to differentiate diploid and triploid types of Paragonimus westermani originating from Japan. It was found that out of 16 restriction enzymes. PstI, HaeIII and RsaI revealed consistently different cleavage patterns between the two types.

Restriction endonuclease analysis of chloroplast DNA from streptomycin-resistant mutants of Nicotiana plumbaginifolia

Genome ◽  
1992 ◽  
Vol 35 (2) ◽  
pp. 220-224 ◽  
Author(s):  
Kin-Ying To ◽  
Yiu-Kay Lai ◽  
Teng-Yung Feng ◽  
Chi-Chang Chen
Keyword(s):  
16S Rrna ◽  
Chloroplast Dna ◽  
Restriction Endonuclease ◽  
Restriction Enzymes ◽  
Restriction Endonuclease Analysis ◽  
Nicotiana Plumbaginifolia ◽  
Streptomycin Resistance ◽  
Rrna Gene ◽  
Resistant Mutants ◽  
Endonuclease Analysis

Chloroplast DNA isolated from wild-type Nicotiana plumbaginifolia and 12 maternally inherited streptomycin-resistant mutants was digested with various restriction enzymes and the resultant patterns were compared. No gross structural alterations of the chloroplast genome were detected in any mutants; however, variant patterns owing to the gain or loss of a restriction site were found in three mutants, SR1007, SR1019, and SR1022. The variant patterns in SR1019 and SR1022 are identical and are the results of mutation in the psbG gene coding for a chloroplast membrane protein G, and that in SR1007 is due to mutation in the 16S rRNA gene. Inheritance of the variant patterns in mutants SR1007 and SR1019 was studied. The results showed that the variant patterns and streptomycin resistance were co-transmitted in reciprocal crosses.Key words: Nicotiana plumbaginifolia, streptomycin resistance, chloroplast DNA, restriction endonuclease analysis, 16S rRNA.

Role of genetic variability in the renin-angiotensin system in diabetic and nondiabetic renal disease

2001 ◽  
Vol 21 (6) ◽  
pp. 580-592 ◽  
Author(s):  
Arnold Boonstra ◽  
Dick de Zeeuw ◽  
Paul E. de Jong ◽  
Gerjan Navis
Keyword(s):  
Genetic Variability ◽  
Renal Disease ◽  
Renin Angiotensin System ◽  
Angiotensin System ◽  
Renin Angiotensin

ROLE OF LIPID PROFILE IN OVARIAN CARCINOMA PATIENTS

2019 ◽  
Vol 3 (8) ◽  
Author(s):  
Dr. Manisha ◽  
Dr. Ruchi Jindal
Keyword(s):  
Ovarian Cancer ◽  
Lipid Profile ◽  
Cancer Patients ◽  
Ovarian Tumor ◽  
Ovarian Cancer Risk ◽  
Low Level ◽  
Medical College ◽  
Group I ◽  
History Of

Background: The term "ovarian cancer" includes several different types of cancer that  arise from cells of the ovary, most commonly, tumors arise from the epithelium or lining cells of the ovary.  Ovarian cancer risk is positively associated with higher consumption of dietary cholesterol and eggs, and inversely associated with a higher intake of vegetables. High consumption of fats may increase circulating estrogen levels, thus increasing the possibility of cell damage and proliferation that is responsible for cancerous growth. Material & Methods: The present study was conducted at Geetanjali Medical College and Hospital, Udaipur (Rajasthan). Total  100 cases (females) attending the obstetrics and gynecology department for some gynecological and other problem  were selected for this study between the age of 40-60 years, who were attending cancer centre at GEETANJALI MEDICAL COLLEGE AND  HOSPITAL, Udaipur (Rajasthan).                GROUP I: - It consisted of healthy females control subjects (n=50) .By routine examination and tests, we ensured that all the subjects were healthy and there were no signs and symptoms or history of ovarian tumor and diseases GROUP II: - It consisted of ovarian cancer females subjects (n=50) with a history of ovarian tumor. Results:   Higher level of cholesterol, LDL, VLDL and low level of HDL are found in ovarian cancer patients. Conclusion: The present study we highlights the importance and role of serum lipid profile in diagnosis, prognosis and recurrence of the disease. The study shows that serum level of cholesterol, LDL, VLDL was elevated in  patients of ovarian cancer while low level of HDL are found in ovarian cancer patients. Key words: lipid profile, ovarian cancer.

scholarly journals Psychosocial Obstacles in Young Children Argumentative Interactions

2021 ◽  
Vol 11 (5) ◽  
pp. 224
Author(s):  
Josephine Convertini
Keyword(s):  
Preschool Children ◽  
Young Children ◽  
Social Dynamics ◽  
Learning Processes ◽  
Self Perception ◽  
Complex Activity ◽  
Group I

Argumentation is an important aspect in the field of education because of its impact on learning processes. At the same time, argumentation is a complex activity in terms of cognitive, relational, emotional and social dynamics. In this paper, I investigate and I describe possible difficulties encountered by children during the argumentative process. The study involves 25 preschool children at a kindergarten engaged in three building tasks. The tasks were video-recorded and the argumentative discussions transcribed. For the aim of this paper, I analyze how argumentation are distributed among participants. I select interactions in which participants apparently do not argue or there are differences in the degree of argumentative participation between participants of the same group. I analyze these interactions and moments of impasse in the argumentative steps. The findings show how the simplicity of solving the task (e.g., when children do not encounter any problem in completing the activity) and the children’s self-perception of their competences in solving the task may have an impact on argumentation activities. Moreover, this perception is co-constructed by children within the interaction. The study contributes to the line of research on designing argumentation and highlights the role of the adult in managing children’s interactions.

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