Dynamics of indigenous bacterial communities associated with crude oil degradation in soil microcosms during nutrient-enhanced bioremediation

Abstract We report kinetics of Assam crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2, both isolated from Assam refinery sediments. The isolates exhibited appreciable degrees of hydrophobicity, emulsification index and biosurfactant production. Crude oil degradation efficiency of isolates was assessed in (1) liquid medium amended with 1% v/v crude oil and (2) microcosm sediments (125 mg crude oil/ 10 g sand). In liquid culture, the biodegradation rate (k) and half-life (t1/2) values were found to be 0.0383 day -1 and 18.09 days for P. aeruginosa AKS1, and 0.0204 day -1 and 33.97 days in case of Bacillus sp. AKS2. In microcosm sand sediments, the estimated biodegradation rate (k) and half-life (t 1/2) values were 0.0138 day -1 and 50 days for P. aeruginosa AKS1, and 0.0113 day -1 and 61.34 days in case of Bacillus sp. AKS2. The level of nutrient treatment in microcosm sand sediment was 125 µg N & 62.5 µg P/g sediment in case of P. aeruginosa AKS1 and 375 µg N & 37.5 µg P/g sediment in case of Bacillus sp. AKS2. In microcosms without inorganic nutrients, biodegradation rate (k) and half-life (t1/2) values were found to be 0.0069 day -1 and 100 days for P. aeruginosa AKS1 and for Bacillus sp. AKS2, the respective values were found to be 0.0046 day -1 and 150.68 days. Our data provides important information for predictive hydrocarbon degradation in liquid medium and contaminated sediments.

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Kinetics of nutrient enhanced crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2 isolated from Guwahati refinery, India

Environmental Pollution ◽

10.1016/j.envpol.2016.06.008 ◽

2016 ◽

Vol 216 ◽

pp. 548-558 ◽

Cited By ~ 13

Author(s):

Bobby Chettri ◽

Arghya Mukherjee ◽

James S. Langpoklakpam ◽

Dhrubajyoti Chattopadhyay ◽

Arvind K. Singh

Keyword(s):

Pseudomonas Aeruginosa ◽

Crude Oil ◽

Oil Degradation ◽

Bacillus Sp ◽

Crude Oil Degradation ◽

Kinetics Of

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Crude oil degradation by microorganisms isolated from the marine environment

Zeitschrift für allgemeine Mikrobiologie ◽

10.1002/jobm.3630130403 ◽

1973 ◽

Vol 13 (4) ◽

pp. 299-306 ◽

Cited By ~ 31

Author(s):

C. E. Cerniglia ◽

J. J. Perry

Keyword(s):

Crude Oil ◽

Marine Environment ◽

Oil Degradation ◽

Crude Oil Degradation

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Investigation of crude oil degradation using metal oxide anode-based microbial fuel cell

Bioresource Technology Reports ◽

10.1016/j.biteb.2020.100449 ◽

2020 ◽

Vol 11 ◽

pp. 100449

Author(s):

Arpita Nandy ◽

Jagoš R. Radović ◽

Breda Novotnik ◽

Mohita Sharma ◽

Stephen R. Larter ◽

...

Keyword(s):

Fuel Cell ◽

Metal Oxide ◽

Crude Oil ◽

Microbial Fuel Cell ◽

Oil Degradation ◽

Crude Oil Degradation ◽

Metal Oxide Anode ◽

Oxide Anode

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Newly Isolated Alkane Hydroxylase and Lipase Producing Geobacillus and Anoxybacillus Species Involved in Crude Oil Degradation

Catalysts ◽

10.3390/catal10080851 ◽

2020 ◽

Vol 10 (8) ◽

pp. 851 ◽

Cited By ~ 1

Author(s):

Durratul Fatini Yusoff ◽

Raja Noor Zaliha Raja Abd Rahman ◽

Malihe Masomian ◽

Mohd Shukuri Mohamad Ali ◽

Thean Chor Leow

Keyword(s):

Crude Oil ◽

Oil Spills ◽

Oil Degradation ◽

Alkane Hydroxylase ◽

Rrna Sequence ◽

16S Rrna Sequence ◽

Crude Oil Degradation ◽

16S Rrna Sequence Analysis ◽

First Time ◽

Poly Aromatic Hydrocarbons

Isolation and studies of novel, crude oil biodegrading thermophilic strains may provide a wider knowledge in understanding their role in petroleum degradation. In this study, the screening of ten new thermophilic strains revealed that all strains were alkane hydroxylase producers and seven of them produced lipase concurrently. Three best strains were characterized and identified through 16S rRNA sequence analysis as Geobacillus sp. D4, Geobacillus sp. D7, and Anoxybacillus geothermalis D9 with GenBank accession numbers MK615934.1, MK615935.1, and MK615936.1, respectively. Gas chromatography (GC) analysis showed that all three strains were able to breakdown various compounds in crude oil such as alkanes, toxic poly-aromatic hydrocarbons (PAHs), organosulfur, carboxylic acids, alkene, resins, organosilicon, alcohol, organochlorine, and ester. For the first time, alkane hydroxylase and lipase activity as well as crude oil degradation by A. geothermalis species were reported. Geobacillus sp. D7 is the best alkane degrader followed by A. geothermalis D9 and Geobacillus sp. D4 with 17.3%, 13.1%, and 12.1% biodegradation efficiency (BE%), respectively. The potential of thermophiles isolated can be explored further for bioremediation of sites polluted by petroleum and oil spills.

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Crude Oil Degrading Fingerprint and the Overexpression of Oxidase and Invasive Genes for n-hexadecane and Crude Oil Degradation in the Acinetobacter pittii H9-3 Strain

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16020188 ◽

2019 ◽

Vol 16 (2) ◽

pp. 188 ◽

Cited By ~ 1

Author(s):

Yang Wang ◽

Qiuyu Wang ◽

Limei Liu

Keyword(s):

Crude Oil ◽

Carbon Sources ◽

Morphological Characteristics ◽

Oil Degradation ◽

16S Rdna Sequence Analysis ◽

Degrading Bacterium ◽

Branched Alkanes ◽

Crude Oil Degradation ◽

Acinetobacter Pittii ◽

The Difference

A crude oil-degrading bacterium named strain H9-3 was isolated from crude oil contaminated soil in the Northeastern area of China. Based on its morphological characteristics and 16S rDNA sequence analysis, strain H9-3 is affiliated to Acinetobacter pittii in the group of Gammaproteobacteria. The strain was efficient in removing 36.8% of the initial 10 g·L - 1 of crude oil within 21 days. GC-MS was performed and a preference was shown for n-C10, n-C11, i-C14, i-C17, i-C34, n-C12, n-C13, n-C14, n-C27, n-C32 and i-C13, over n-C16, n-C18–C22, n-C24–n-C31, and n-C36. This can be regarded as the specific fingerprint for crude oil degradation by strain H9-3 of Acinetobacter pittii. In addition to crude oil, it was shown that soybean oil and phenols can be utilized as carbon sources by strain H9-3. It was also shown that aniline and α -naphthol cannot be utilized for growth, but they can be tolerated by strain H9-3. Methylbenzene was neither utilized nor tolerated by strain H9-3. Although n-hexadecane was not preferentially consumed by strain H9-3, during culture with crude oil, it could be utilized for growth when it is the sole carbon source. The degradation of some branched alkanes (i-C14, i-C17 and i-C34) and the preferential degradation of crude oil over phenols could be used as a reference for distinguishing A. pittii from A. calcoaceticus. The difference in gene expression was very significant and was induced by diverse carbon sources, as shown in the qRT-PCR results. The oxidation and adhesion events occurred at high frequency during alkane degration by Acinetobacter pittii strain H9-3 cells.

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