Chemical–physical and in vivo evaluations of a self-assembling amphiphilic peptide as an injectable hydrogel scaffold for biomedical applications

2012 ◽  
Vol 28 (1) ◽  
pp. 3-15 ◽  
Author(s):  
Roberto Solaro ◽  
Michele Alderighi ◽  
Maria C Barsotti ◽  
Antonella Battisti ◽  
Mario Cifelli ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Hydrogel Scaffold ◽  
Injectable Hydrogel ◽  
Self Assembling ◽  
Amphiphilic Peptide

scholarly journals Overview of DNA Self-Assembling: Progresses in Biomedical Applications

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 268 ◽  
Author(s):  
Andreia Jorge ◽  
Ramon Eritja
Keyword(s):  
Programming Languages ◽  
Biomedical Applications ◽  
Great Promise ◽  
Dna Nanostructures ◽  
Modular Assembly ◽  
Molecular Programming ◽  
Self Assembling ◽  
Theranostic Agents ◽  
Assembly Principles

Molecular self-assembling is ubiquitous in nature providing structural and functional machinery for the cells. In recent decades, material science has been inspired by the nature’s assembly principles to create artificially higher-order structures customized with therapeutic and targeting molecules, organic and inorganic fluorescent probes that have opened new perspectives for biomedical applications. Among these novel man-made materials, DNA nanostructures hold great promise for the modular assembly of biocompatible molecules at the nanoscale of multiple shapes and sizes, designed via molecular programming languages. Herein, we summarize the recent advances made in the designing of DNA nanostructures with special emphasis on their application in biomedical research as imaging and diagnostic platforms, drug, gene, and protein vehicles, as well as theranostic agents that are meant to operate in-cell and in-vivo.

Bioimaging Applications of Non-Lamellar Liquid Crystalline Nanoparticles

2021 ◽  
Vol 21 (5) ◽  
pp. 2742-2759
Author(s):  
Sergio Murgia ◽  
Stefania Biffi ◽  
Marco Fornasier ◽  
Vito Lippolis ◽  
Giacomo Picci ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Liquid Crystalline ◽  
Colloidal Nanoparticles ◽  
Self Assembling ◽  
Complex Architectures ◽  
Imaging Probes ◽  
Bicontinuous Cubic ◽  
Liquid Crystalline Nanoparticles

Self-assembling processes of amphiphilic lipids in water give rise to complex architectures known as lyotropic liquid crystalline (LLC) phases. Particularly, bicontinuous cubic and hexagonal LLC phases can be dispersed in water forming colloidal nanoparticles respectively known as cubosomes and hexosomes. These non-lamellar LLC dispersions are of particular interest for pharmaceutical and biomedical applications as they are potentially non-toxic, chemically stable, and biocompatible, also allowing encapsulation of large amounts of drugs. Furthermore, conjugation of specific moieties enables their targeting, increasing therapeutic efficacies and reducing side effects by avoiding exposure of healthy tissues. In addition, as they can be easy loaded or functionalized with both hydrophobic and hydrophilic imaging probes, cubosomes and hexosomes can be used for the engineering of multifunctional/theranostic nanoplatforms. This review outlines recent advances in the applications of cubosomes and hexosomes for in vitro and in vivo bioimaging.

scholarly journals In vivo safety and efficacy testing of a thermally triggered injectable hydrogel scaffold for bone regeneration and augmentation in a rat model

Oncotarget ◽  
2018 ◽  
Vol 9 (26) ◽  
pp. 18277-18295 ◽  
Author(s):  
Abbey A. Thorpe ◽  
Christine Freeman ◽  
Paula Farthing ◽  
Jill Callaghan ◽  
Paul V. Hatton ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Rat Model ◽  
Hydrogel Scaffold ◽  
Injectable Hydrogel ◽  
Safety And Efficacy ◽  
Thermally Triggered

Covalent Cell‐Loading Injectable Hydrogel Scaffold Significantly Promotes Tissue Regeneration In Vivo Compared with a Conventional Physical Cell‐Loading Hydrogel Scaffold

2021 ◽  
Vol 5 (2) ◽  
pp. 2000106
Author(s):  
Yuka Kimura ◽  
Seika Aoyama ◽  
Natsumi Ueda ◽  
Tokitaka Katayama ◽  
Kimika Ono ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Cell Loading ◽  
Hydrogel Scaffold ◽  
Injectable Hydrogel

scholarly journals Self-Assembling RADA16-I Peptide Hydrogel Scaffold Loaded with Tamoxifen for Breast Reconstruction

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Huimin Wu ◽  
Ting Zhou ◽  
Lin Tian ◽  
Zhengchao Xia ◽  
Feng Xu
Keyword(s):  
3D Culture ◽  
Fat Absorption ◽  
Breast Cancer Patients ◽  
Hydrogel Scaffold ◽  
Hydrogel Scaffolds ◽  
In Vivo Experiments ◽  
Self Assembling ◽  
Apoptosis Related Protein ◽  
Mcf 7

More and more breast cancer patients prefer autologous fat tissue transfer following lumpectomy to maintain perfect female characteristics. However, the outcome was not satisfactory due to the transplanted fat absorption. In this study, we prepared two RADA16-I peptide scaffolds with and without tamoxifen. Both scaffolds were transparent, porous, and hemisphere-shaped. The hADSCs isolated from liposuction were attached to the scaffold. The growth inhibition of the hADSCs induced by TAM in 2-demensional (2D) culture was higher than that in TAM-loaded hydrogel scaffold 3D culture (P<0.05); however, the same outcomes were not observed in MCF-7 cells. Correspondingly, the apoptosis of the hADSCs induced by TAM was significantly increased in 2D culture compared to that in scaffold 3D culture (P<0.05). Yet the outcomes of the aoptosis in MCF-7 were contrary. Apoptosis-related protein Bcl-2 was involved in the process. In vivo experiments showed that both scaffolds formed a round mass after subcutaneous implantation and it retained its shape after being pressed slightly. The implantation had no effect on the weight and activity of the animals. The results suggested that TAM-loaded RADA16-I hydrogel scaffolds both provide support for hADSCs cells attachment/proliferation and retain cytotoxic effect on MCF-7 cells, which might be a promising therapeutic breast tissue following lumpectomy.

In vitro and in vivo assessments of a 3-(3,4-dihydroxyphenyl)-2-propenoic acid bioconjugated gelatin-based injectable hydrogel for biomedical applications

2015 ◽  
Vol 3 (7) ◽  
pp. 1230-1244 ◽  
Author(s):  
S. Thirupathi Kumara Raja ◽  
T. Thiruselvi ◽  
R. Aravindhan ◽  
Asit Baran Mandal ◽  
A. Gnanamani
Keyword(s):  
Drug Delivery ◽  
Wound Healing ◽  
Biomedical Applications ◽  
Adhesive Material ◽  
Injectable Hydrogel ◽  
Propenoic Acid ◽  
Multifunctional Properties

A novel bioconjugated injectable hydrogel with multifunctional properties for wound healing and drug delivery: a biomimetic adhesive material.

scholarly journals Feasibility of a self‐assembling peptide hydrogel scaffold for meniscal defect: An in vivo study in a rabbit model

2020 ◽  
Vol 39 (1) ◽  
pp. 165-176
Author(s):  
Nobuhiro Okuno ◽  
Shuhei Otsuki ◽  
Jo Aoyama ◽  
Kosuke Nakagawa ◽  
Tomohiko Murakami ◽  
...  
Keyword(s):  
Rabbit Model ◽  
In Vivo Study ◽  
Hydrogel Scaffold ◽  
Self Assembling ◽  
Peptide Hydrogel

Biomedical applications: Pitfalls in the practice

1994 ◽  
Vol 52 ◽  
pp. 378-379
Author(s):  
J. D. Shelburne ◽  
Peter Ingram ◽  
Victor L. Roggli ◽  
Ann LeFurgey
Keyword(s):  
Operating Room ◽  
Liquid Nitrogen ◽  
Lung Tissue ◽  
Biomedical Applications ◽  
Microprobe Analysis ◽  
Tissue Sample ◽  
Cellular Level ◽  
Tissue Samples ◽  
Asbestos Fibers

At present most medical microprobe analysis is conducted on insoluble particulates such as asbestos fibers in lung tissue. Cryotechniques are not necessary for this type of specimen. Insoluble particulates can be processed conventionally. Nevertheless, it is important to emphasize that conventional processing is unacceptable for specimens in which electrolyte distributions in tissues are sought. It is necessary to flash-freeze in order to preserve the integrity of electrolyte distributions at the subcellular and cellular level. Ideally, biopsies should be flash-frozen in the operating room rather than being frozen several minutes later in a histology laboratory. Electrolytes will move during such a long delay. While flammable cryogens such as propane obviously cannot be used in an operating room, liquid nitrogen-cooled slam-freezing devices or guns may be permitted, and are the best way to achieve an artifact-free, accurate tissue sample which truly reflects the in vivo state. Unfortunately, the importance of cryofixation is often not understood. Investigators bring tissue samples fixed in glutaraldehyde to a microprobe laboratory with a request for microprobe analysis for electrolytes.

Biological and biomedical applications of collagenous biomaterials

1990 ◽  
Vol 48 (3) ◽  
pp. 856-857
Author(s):  
Yasushi P. Kato ◽  
Michael G. Dunn ◽  
Frederick H. Silver ◽  
Arthur J. Wasserman
Keyword(s):  
Biomedical Applications ◽  
Soft Tissues ◽  
Collagen Fibers ◽  
Bone Collagen ◽  
Cover Slip ◽  
Fibroblast Migration ◽  
Cellular Expression ◽  
Defect Repair

Collagenous biomaterials have been used for growing cells in vitro as well as for augmentation and replacement of hard and soft tissues. The substratum used for culturing cells is implicated in the modulation of phenotypic cellular expression, cellular orientation and adhesion. Collagen may have a strong influence on these cellular parameters when used as a substrate in vitro. Clinically, collagen has many applications to wound healing including, skin and bone substitution, tendon, ligament, and nerve replacement. In this report we demonstrate two uses of collagen. First as a fiber to support fibroblast growth in vitro, and second as a demineralized bone/collagen sponge for radial bone defect repair in vivo.For the in vitro study, collagen fibers were prepared as described previously. Primary rat tendon fibroblasts (1° RTF) were isolated and cultured for 5 days on 1 X 15 mm sterile cover slips. Six to seven collagen fibers, were glued parallel to each other onto a circular cover slip (D=18mm) and the 1 X 15mm cover slip populated with 1° RTF was placed at the center perpendicular to the collagen fibers. Fibroblast migration from the 1 x 15mm cover slip onto and along the collagen fibers was measured daily using a phase contrast microscope (Olympus CK-2) with a calibrated eyepiece. Migratory rates for fibroblasts were determined from 36 fibers over 4 days.

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