Fabrication, characterization, and in vitro study of zinc substituted hydroxyapatite/silk fibroin composite coatings on titanium for biomedical applications

2017 ◽  
Vol 32 (3) ◽  
pp. 399-409 ◽  
Author(s):  
Zhenyu Zhong ◽  
Jun Ma
Keyword(s):  
Silk Fibroin ◽  
Composite Coatings ◽  
In Vitro Study ◽  
Biological Behavior ◽  
Apatite Formation ◽  
Hydroxyapatite Coatings ◽  
High Bone ◽  
Biomimetic Hydroxyapatite

Zinc substituted hydroxyapatite/silk fibroin composite coatings were deposited on titanium substrates at room temperature by electrophoretic deposition. Microscopic characterization of the synthesized composite nanoparticles revealed that the particle size ranged 50–200 nm, which increased a little after zinc substitution. The obtained coatings maintained the phase of hydroxyapatite and they could induce fast apatite formation in simulated body fluid, indicating high bone activity. The cell culturing results showed that the biomimetic hydroxyapatite coatings could regulate adhesion, spreading, and proliferation of osteoblastic cells. Furthermore, the biological behavior of the zinc substituted hydroxyapatite coatings was found to be better than the bare titanium without coatings and hydroxyapatite coatings without zinc, increasing MC3T1-E1 cell differentiation in alkaline phosphatase expression.

scholarly journals Fabrication and Characterization of Antimicrobial Magnetron Cosputtered TiO2/Ag/Cu Composite Coatings

Coatings ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 473
Author(s):  
Dilyana Gospodonova ◽  
Iliana Ivanova ◽  
Todorka Vladkova
Keyword(s):  
Antimicrobial Activity ◽  
Composite Coatings ◽  
Surface Characteristics ◽  
Standard Test ◽  
Most Probable Number ◽  
Bacterial Strains ◽  
Probable Number ◽  
Fabrication And Characterization

The aim of this study was to prepare TiO2/Ag/Cu magnetron co-sputtered coatings with controlled characteristics and to correlate them with the antimicrobial activity of the coated glass samples. The elemental composition and distribution, surface morphology, wettability, surface energy and its component were estimated as the surface characteristics influencing the bioadhesion. Well expressed, specific, Ag/Cu concentration-dependent antimicrobial activity in vitro was demonstrated toward Gram-negative and Gram-positive standard test bacterial strains both by diffusion 21 assay and by Most Probable Number of surviving cells. Direct contact and eluted silver/coper nanoparticles killing were experimentally demonstrated as a mode of the antimicrobial action of the studied TiO2/Ag/Cu thin composite coatings. It is expected that they would ensure a broad spectrum bactericidal activity during the indwelling of the coated medical devices and for at least 12 h after that, with the supposition that the benefits will be over a longer time.

Preparation and in vitro characterization of aerosol-deposited hydroxyapatite coatings with different surface roughnesses

2011 ◽  
Vol 257 (17) ◽  
pp. 7792-7799 ◽  
Author(s):  
Byung-Dong Hahn ◽  
Dong-Soo Park ◽  
Jong-Jin Choi ◽  
Jungho Ryu ◽  
Woon-Ha Yoon ◽  
...  
Keyword(s):  
In Vitro Characterization ◽  
Hydroxyapatite Coatings

Synthesis and characterization of novel PUFA esters exhibiting potential anticancer activities: An in vitro study

2011 ◽  
Vol 46 (10) ◽  
pp. 4878-4886 ◽  
Author(s):  
Azmat Ali Khan ◽  
Mahboob Alam ◽  
Saba Tufail ◽  
Jamal Mustafa ◽  
Mohammad Owais
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Synthesis And Characterization ◽  
Anticancer Activities

scholarly journals In Vitro MCF-7 Cells Apoptosis Analysis of Carboplatin Loaded Silk Fibroin Particles

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1110
Author(s):  
Nanyak Galam ◽  
Pinar Tulay ◽  
Terin Adali
Keyword(s):  
Breast Cancer ◽  
Silk Fibroin ◽  
Drug Carrier ◽  
Release Studies ◽  
Apoptotic Effect ◽  
Student’S T ◽  
Drug Carrier System ◽  
Mcf 7

Breast cancer ranks as the fifth leading cause of death worldwide. Chemotherapy is commonly used directly or as neo-adjuvant therapy for the management of breast cancer with its attendant adverse effects, underscoring the need to develop biocompatible bioactive compounds for pharmacological applications. The aim of this study is to encapsulate carboplatin (CP) with silk fibroin protein (SF) by using an ionic gelation method as a drug carrier system and assess the apoptotic effect on MCF-7 breast cancer cells during in vitro studies. The characterization of silk fibroin encapsulated carboplatin (SFCP) microparticles was analyzed by FTIR spectrophotometer, SEM, Mastersizer, and biodegradation methods. The encapsulation efficiency and release profile of SFCP microparticles were analyzed by an indirect UV–Vis spectrophotometric method. An apoptotic screening of MCF-7 cells was carried out with 10–200 µg/mL CP loaded SFCP, which were cultured for 24, 48, and 72 h. Data were analyzed using the Student’s t test and analysis of variance. FTIR and drug release studies confirmed an interaction of silk fibroin with the carboplatin moiety. SFCP showed successful encapsulation of the carboplatin moiety. Apoptotic screening showed a dose dependent increase in absorbance, indicating significant cell death (p < 0.05). Thus, the direct apoptotic effect of SFCP microparticles on MCF-7 was confirmed.

Preparation and characterization of dopamine-induced biomimetic hydroxyapatite coatings on the AZ31 magnesium alloy

2015 ◽  
Vol 281 ◽  
pp. 82-88 ◽  
Author(s):  
Bingpeng Lin ◽  
Mei Zhong ◽  
Chengdong Zheng ◽  
Lin Cao ◽  
Dengli Wang ◽  
...  
Keyword(s):  
Magnesium Alloy ◽  
Az31 Magnesium Alloy ◽  
Hydroxyapatite Coatings ◽  
Biomimetic Hydroxyapatite

In vivo and in vitro trimethadione oxidation activity of the liver from various animal species including mouse, hamster, rat, rabbit, dog, monkey and human

1999 ◽  
Vol 18 (1) ◽  
pp. 12-16 ◽  
Author(s):  
E Tanaka ◽  
A Ishikawa ◽  
T Horie
Keyword(s):  
Animal Species ◽  
In Vitro Study ◽  
Metabolic Clearance ◽  
Oxidation Activity ◽  
In Vitro Characterization ◽  
Demethylase Activity ◽  
Total Body

Trimethadione (TMO) has the properties required of a probe drug for the evaluation of hepatic drug-oxidizing capacity and, in this study, we have summarized the in vivo and in vitro metabolism of TMO in various animal species including mouse, hamster, rat, rabbit, dog, monkey and human. In the in vivo study, the plasma TMO level was measured after intravenous or oral (human) administration of TMO at a dose of 4 mg/kg to various animal species. The rate of TMO metabolic clearance in these animal species in vivo was in the order mouse > hamster >rat>rabbit>dog>monkey>human. In the in vitro study, species differences were observed in the cytochrome P450 (P450) content and drug-oxidizing enzyme activity. The content of P450 was monkey> mouse>dog>rabbit>hamster>rat>human. On the other hand, TMO N-demethylation was in the order mouse >hamster >rat >rabbit>dog>monkey>human. There was a good correlation between the mean total body clearance of TMO ( in vivo)andthemeanTMON-demethylase activity ( in vitro) (y=1.7×+0.11, r=0.965, P<0.001). These results show that TMO is a probe agent with metabolic and pharmacokinetic characteristics making it attractive for the in vivo and in vitro characterization of metabolic activity in various animal species.

Evaluation of surface modification techniques on the ability of apatite formation and corrosion behavior in synthetic body fluid: An in vitro study

2021 ◽  
Vol 22 ◽  
pp. 100866
Author(s):  
Cosmin M. Cotrut ◽  
Ionut C. Ionescu ◽  
Elena Ungureanu ◽  
Andrei Berbecaru ◽  
Raluca I. Zamfir ◽  
...  
Keyword(s):  
Surface Modification ◽  
Corrosion Behavior ◽  
Body Fluid ◽  
In Vitro Study ◽  
Vitro Study ◽  
Apatite Formation ◽  
Surface Modification Techniques
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