Regeneration of Periodontal Tissues

1988 ◽  
Vol 2 (2) ◽  
pp. 223-227 ◽  
Author(s):  
M. Shimono ◽  
T. Inoue ◽  
T. Yamamura
Keyword(s):  
Connective Tissue ◽  
Granulation Tissue ◽  
Periodontal Ligament ◽  
Alveolar Bone ◽  
Gingival Tissue ◽  
Junctional Epithelium ◽  
Cavity Preparation ◽  
Periodontal Tissues ◽  
Tissue Interface ◽  
Cut Surface

To elucidate the regenerative capability of the periodontal tissues, we carried out two experiments: (1) Regeneration of the gingival tissue following gingivectomy in rats. Ultrastructurally, regenerating junctional epithelium was similar in morphology to that of untreated animals and appeared to attach to the enamel after five days. Basal lamina and hemidesmosomes were produced faster at the enamel interface than at the connective tissue interface. Gingival tissue was completely regenerated seven days after the gingivectomy. (2) Regeneration of the cementum, periodontal ligament, and alveolar bone following intradentinal cavity preparation in dogs. In the early stages, the cavity was filled with an exudate and granulation tissue. Seven days after the operation, osteoblasts and cementoblasts were arranged regularly on the cut surface of the alveolar bone and dentin, respectively. Newly formed bone and cementum, and periodontal ligament grew to resemble pre-existing bone and cementum after 28-42 days. From these results, it is suggested that the periodontal tissues have an extremely high capability of regeneration.

scholarly journals The distribution of fibronectin in rat tooth and periodontal tissues: an immunofluorescence study using a monoclonal antibody.

1984 ◽  
Vol 32 (6) ◽  
pp. 565-572 ◽  
Author(s):  
N S Connor ◽  
J E Aubin ◽  
A H Melcher
Keyword(s):  
Monoclonal Antibody ◽  
Connective Tissue ◽  
Blood Vessel ◽  
Indirect Immunofluorescence ◽  
Periodontal Ligament ◽  
Alveolar Bone ◽  
Immunofluorescence Study ◽  
Connective Tissues ◽  
Adult Rat ◽  
Periodontal Tissues

The distribution of fibronectin (FN) in longitudinal, buccolingual sections of decalcified adult rat periodontium and teeth was studied by indirect immunofluorescence using a monoclonal antibody. FN was present in virtually all regions of the periodontium, including the gingiva, periodontal ligament, many blood vessel walls, alveolar bone, incisor and molar predentine and dentine, and molar acellular and cellular cementum. The cementum of the incisor, ameloblasts, stratum intermedium and stellate reticulum, and the connective tissue of the pulp and the surface of ondontoblasts facing the pulp in the incisor and molar were not labeled for FN. FN distribution was not always uniform either within a given connective tissue or between different connective tissues of the same organ.

scholarly journals Mechanosensitive Piezo1 in Periodontal Ligament Cells Promotes Alveolar Bone Remodeling During Orthodontic Tooth Movement

2021 ◽  
Vol 12 ◽  
Author(s):  
Yukun Jiang ◽  
Yuzhe Guan ◽  
Yuanchen Lan ◽  
Shuo Chen ◽  
Tiancheng Li ◽  
...  
Keyword(s):  
Bone Remodeling ◽  
Periodontal Ligament ◽  
Alveolar Bone ◽  
Tooth Movement ◽  
Critical Role ◽  
Orthodontic Tooth Movement ◽  
Chemical Signals ◽  
Periodontal Tissues ◽  
Alveolar Bone Remodeling ◽  
Pdl Cells

Orthodontic tooth movement (OTM) is a process depending on the remodeling of periodontal tissues surrounding the roots. Orthodontic forces trigger the conversion of mechanical stimuli into intercellular chemical signals within periodontal ligament (PDL) cells, activating alveolar bone remodeling, and thereby, initiating OTM. Recently, the mechanosensitive ion channel Piezo1 has been found to play pivotal roles in the different types of human cells by transforming external physical stimuli into intercellular chemical signals. However, the function of Piezo1 during the mechanotransduction process of PDL cells has rarely been reported. Herein, we established a rat OTM model to study the potential role of Piezo1 during the mechanotransduction process of PDL cells and investigate its effects on the tension side of alveolar bone remodeling. A total of 60 male Sprague-Dawley rats were randomly assigned into three groups: the OTM + inhibitor (INH) group, the OTM group, and the control (CON) group. Nickel-titanium orthodontic springs were applied to trigger tooth movement. Mice were sacrificed on days 0, 3, 7, and 14 after orthodontic movement for the radiographic, histological, immunohistochemical, and molecular biological analyses. Our results revealed that the Piezo1 channel was activated by orthodontic force and mainly expressed in the PDL cells during the whole tooth movement period. The activation of the Piezo1 channel was essential for maintaining the rate of orthodontic tooth movement and facilitation of new alveolar bone formation on the tension side. Reduced osteogenesis-associated transcription factors such as Runt-related transcription factor 2 (RUNX2), Osterix (OSX), and receptor activator of nuclear factor-kappa B ligand (RANKL)/osteoprotegerin (OPG) ratio were examined when the function of Piezo1 was inhibited. In summary, Piezo1 plays a critical role in mediating both the osteogenesis and osteoclastic activities on the tension side during OTM.

scholarly journals PENGGUNAAN DOKSISIKLIN HYCLATE SEBAGAI INHIBITOR MATRIKS METALLOPROTEINASE PADA TERAPI TAMBAHAN PERIODONTITIS

SAINSTIS ◽  
2013 ◽  
Author(s):  
Risma Aprinda Kristanti
Keyword(s):  
Tissue Damage ◽  
Host Response ◽  
Alveolar Bone ◽  
Periodontal Tissue ◽  
Junctional Epithelium ◽  
Inhibit Activity ◽  
Collagenase Activity ◽  
Periodontal Tissues ◽  
Mmp Inhibitor ◽  
Extracellular Matrix Molecules

<p>Terbit.com  Daily  has  reported  that  the  prevalence  of  periodontitis increases related with age person. Found that 35,7% of patients with periodontitis in the group 30-39 years old to 66,5% in the group 50-59 years old, increase</p> <p>89,2% in the group 80-90 years old. Periodontitis that commonly found is a chronic periodontitis which is occurs in individuals older than 45 years, but can also be found in children.</p> <p>The main characteristic of periodontitis is the damage of the periodontal connective tissue, alveolar bone, and the movement of the junctional epithelium to the apical. Bacterial components may directly or indirectly cause tissue damage. Inflammatory    mediators    product    such    as    proteinases,    cytokines,    and prostaglandins are part of the host response that can also cause tissue damage. Matrix metalloproteinase (MMP) is a proteinase that can cause periodontal tissue damage by disrupting the extracellular matrix molecules in periodontal tissues. Since is known that MMP has a role in various pathological processes, therapy to inhibit activity MMP in pathological processes has developed. MMP inhibitors will be a useful adjunct therapy for the treatment of periodontal disease. Many products have been introduced as an MMP inhibitor, for example: tetracycline</p> <p>and various derivatives, such as doxycycline and minocycline which are capable of inhibiting the activity of several classes of MMPs. Doxycycline has the ability to inhibit the increase in collagenase activity that is pathological in subgingival and prevent periodontitis to become more severe.</p>

Root-Surface Caries in Rats and Humans: Inhibition by a Non-Antimicrobial Property of Tetracyclines

1998 ◽  
Vol 12 (1) ◽  
pp. 43-50 ◽  
Author(s):  
N.S. Ramamurthy ◽  
K.L. Schroeder ◽  
T.F. McNamara ◽  
A.J. Gwinnett ◽  
R.T. Evans ◽  
...  
Keyword(s):  
Alveolar Bone ◽  
Gingival Crevicular Fluid ◽  
Antimicrobial Property ◽  
Subsequent Development ◽  
Gingival Recession ◽  
Periodontal Pathogen ◽  
Gingival Tissue ◽  
Root Caries ◽  
Chewing Tobacco ◽  
Periodontal Tissues

The incidence of root caries has been found to increase as the population ages and as edentulism becomes less prevalent due to improved dental awareness and care, and as exposure of roots due to gingival recession has also increased in the elderly. The mechanism of root caries is thought to be mediated by both bacterial and mammalian proteases produced by plaque and the periodontal tissues, respectively. In the current study, a rat model of periodontal disease was used in which gnotobiotic rats were infected intra-orally with a periodontal pathogen ( P. gingivalis). Infecting the rats with P. gingivalis increased the collagenase activity in the gingival tissue in association with severe alveolar bone loss. Treating P. gingivalis-infected rats with doxycycline or CMT-1 prevented the destruction of the periodontium by MMPs, thus preventing exposure of roots to subgingival bacterial plaque and host tissue collagenases and the subsequent development of root caries. In addition, a low-dose doxycycline (LDD, 20 mg bid, non-antimicrobial dose) for 3 months was used in humans predisposed to increased root caries as the result of heavy use of smokeless (chewing) tobacco, causing gingival recession, subgingival plaque accumulation with Gram-negative bacteria, increased gingival crevicular fluid flow (GCF), and elevated GCF collagenase. Daily administration of LDD in smokeless tobacco patients reduced the GCF collagenase and prevented the further development of root caries.

scholarly journals The Role of Noncoding RNAs in Osteogenic Differentiation of Human Periodontal Ligament Stem Cells

2021 ◽  
Vol 6 ◽  
pp. 247275122199922
Author(s):  
Paras Ahmad ◽  
Martin J. Stoddart ◽  
Elena Della Bella
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Periodontal Ligament ◽  
Noncoding Rna ◽  
Molecular Mechanisms ◽  
Alveolar Bone ◽  
Noncoding Rnas ◽  
Tissue Destruction ◽  
Periodontal Ligament Stem Cells ◽  
Periodontal Tissues

Chronic inflammatory diseases, including periodontitis, are the most common causes of bone tissue destruction. Periodontitis often leads to loss of connective tissue homeostasis and reduced alveolar bone levels. Human periodontal ligament stem cells (PDLSCs), a population of multipotent stem cells derived from periodontal ligament tissues, are considered as candidate cells for the regeneration of alveolar bone and periodontal tissues. Periodontitis impairs the osteogenic differentiation of human PDLSCs. Noncoding RNAs (ncRNAs), including long noncoding RNA (lncRNA), microRNA (miRNA), and circular RNA (circRNA), have been proposed as vital regulators influencing several differentiation processes including bone regeneration. Still, the molecular mechanisms of ncRNAs regulating osteogenic differentiation of human PDLSCs remain poorly understood. Exploring the influence of ncRNAs in the process of osteogenic differentiation of human PDLSCs may provide novel therapeutic strategies for tissue regeneration as the regeneration of the lost periodontium is the ultimate goal of periodontal therapy.

Tissue engineering in periodontology: Biological mediators for periodontal regeneration

2019 ◽  
Vol 42 (5) ◽  
pp. 241-257 ◽  
Author(s):  
Daniela Carmagnola ◽  
Gaia Pellegrini ◽  
Claudia Dellavia ◽  
Lia Rimondini ◽  
Elena Varoni
Keyword(s):  
Tissue Engineering ◽  
Periodontal Ligament ◽  
Alveolar Bone ◽  
State Of The Art ◽  
Periodontal Regeneration ◽  
Functional System ◽  
Bioactive Molecules ◽  
Comprehensive Review ◽  
Periodontal Tissues ◽  
Temporal And Spatial

Teeth and the periodontal tissues represent a highly specialized functional system. When periodontal disease occurs, the periodontal complex, composed by alveolar bone, root cementum, periodontal ligament, and gingiva, can be lost. Periodontal regenerative medicine aims at recovering damaged periodontal tissues and their functions by different means, including the interaction of bioactive molecules, cells, and scaffolds. The application of growth factors, in particular, into periodontal defects has shown encouraging effects, driving the wound healing toward the full, multi-tissue periodontal regeneration, in a precise temporal and spatial order. The aim of the present comprehensive review is to update the state of the art concerning tissue engineering in periodontology, focusing on biological mediators and gene therapy.

Effects of Paste Type Calcium Sulfate on the Periodontal Healing of 3-Wall Intrabony Defects in Dogs

2006 ◽  
Vol 309-311 ◽  
pp. 203-206 ◽  
Author(s):  
Tae Gyun Kim ◽  
S.J. Hyun ◽  
Ui Won Jung ◽  
C.S. Kim ◽  
Chong Kwan Kim ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Calcium Sulfate ◽  
Alveolar Bone ◽  
Junctional Epithelium ◽  
New Bone Formation ◽  
Intrabony Defects ◽  
Epithelial Migration ◽  
Intrabony Defect ◽  
Tissue Attachment ◽  
Periodontal Healing

The purpose of this study is to evaluate the effect of paste type calcium sulfate on the epithelial migration, alveolar bone regeneration, cementum formation and gingival connective tissue attachment in intrabony defect in dogs. These results suggest that the use of paste type calcium sulfate in 3-wall intrabony defects has significant effect on new cementum formation , but doesn't have any significant effect on the prevention of junctional epithelium migration and new bone formation. As a result, the paste type calcium sulfate that is used in this study is suggested to be the material that can have a significant effect on the periodontal healing, if its biocompatibility is improved.

Expression of MMP-8 and MMP-13 mRNAs in Rat Periodontium during Tooth Eruption

2002 ◽  
Vol 81 (10) ◽  
pp. 673-678 ◽  
Author(s):  
M. Tsubota ◽  
Y. Sasano ◽  
I. Takahashi ◽  
M. Kagayama ◽  
H. Shimauchi
Keyword(s):  
Periodontal Ligament ◽  
Alveolar Bone ◽  
Small Population ◽  
Tooth Eruption ◽  
Periodontal Ligament Cells ◽  
Rt Pcr ◽  
Collagen Types ◽  
Paraffin Sections ◽  
Periodontal Tissues

The present study was designed to investigate mRNA expression of matrix metalloproteinase-8 (MMP-8) and MMP-13 in forming periodontium during tooth eruption in the rat. RT-PCR for the decalcified paraffin sections indicated expression of MMP-8 and MMP-13 in the periodontal tissues. In situ hydridization demonstrated expression of MMP-8 in osteoblasts, osteocytes, periodontal ligament cells, cementoblasts, and cementocytes along with collagen types I and III. In contrast, transcripts of MMP-13 were confined to a small population of osteoblasts and osteocytes in alveolar bone. The results suggested that MMP-8 may be involved in remodeling the periodontium during tooth eruption, and its expression may be coordinated with that of collagen types I and III, whereas the participation of MMP-13 may be rather limited.

scholarly journals SYNERGISTIC EFFECT OF QUERCETIN AND EPIGALLOCATECHIN-3-GALLATE AS AGENTS TO PREVENT CONNECTIVE TISSUE DISINTEGRATION IN THE PERIODONTIUM OF RATS UNDER SYSTEMIC AND LOCAL ADMINISTRATION OF LIPOPOLISACCHARIDE OF SALMONELLA TYPHI

2019 ◽  
Vol 23 (5-6) ◽  
pp. 42-44
Author(s):  
A.M. Yelins’ka ◽  
V.O. Kostenko
Keyword(s):  
Connective Tissue ◽  
Soft Tissues ◽  
Alveolar Bone ◽  
Effective Means ◽  
Organic Matrix ◽  
Water Soluble ◽  
Soluble Form ◽  
Experimental Conditions ◽  
Periodontal Tissues ◽  
White Rats

The aim of the present study was to investigate the co-effect produced by water-soluble form of quercetin and epigallocatechin-3-gallate (EGCG) on biochemical markers of periodontal organic matrix depolimerization under systemic administration and local application of S. typhi lipopolisaccharide (LPS). The studies were conducted on 30 white rats of the Wistar line weighing 180-220 g, divided into 5 groups: the 1st included intact animals, the 2nd was made up of animals after the combined systemic and local LPS administration, the 3rd and 4th groups included animals, which were being given injections with water-soluble form of quercetin (10 mg / kg) and EGCG (21.1 mg / kg) respectively 3 times a week, starting on the 30th day of the systemic LPS administration, and the 5th group involved rats, which were injected with co-administered water-soluble form of quercetin and EGCG. It has been found out that the co-effect produced by quercetin and EGCG under systemic and local LPS administration is accompanied with reduced concentration of N-acetylneuraminic acid (NANA) by 31.8 and 32.8% respectively in the soft periodontal tissues compared with values for the animals received separate quercetin and EGCG during the experiment. However, no differences have been detected between the groups exposed to combined or separate action of the above mentioned agents in the experiment when assessing free hydroxyproline (FHP) and glycosaminoglycans (GAGs) content in the soft tissues of periodontium. At the same time combined use of quercetin and EGCG under experimental conditions led to the decrease in the FHP content in the alveolar bone by 24.5 and 20.2% respectively compared with values for the animals received separate quercetin and EGCG. NANA concentration was reduced by 35.0 and 41.3% respectively. Thus, the co-administration of water-soluble form of quercetin and epigallocatechin-3-gallate under systemic and local introducing of S. typhi lipopolysaccharide has been proven to be more effective means for preventing and correcting periodontal connective tissue disruption than this occurs at separate administration of each of the polyphenols.

The Effect of Enamel Matrix Derivate Proteins EMD on Ortodontically Induced Bone Resorption Based on Mini-implants

2017 ◽  
Vol 68 (7) ◽  
pp. 1457-1459
Author(s):  
Alexandru Vlasa ◽  
Carmen Biris ◽  
Mariana Pacurar ◽  
Anamaria Bud ◽  
Eugen Bud ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Periodontal Ligament ◽  
Normal Development ◽  
Alveolar Bone ◽  
Enamel Matrix ◽  
Periodontal Tissues ◽  
Mini Implants ◽  
Human Enamel ◽  
Enamel Proteins ◽  
High Degree

Amelogenine protein is the major component of the continuously secreted enamel extracellular matrix that controls the mineralization of enamel crystals. EmdogainTM is an extract of porcine fetal tooth material, a product based on the high degree of homology between porcine and human enamel proteins, composed primarily of amelogenine protein. It was created to promote the regeneration of periodontal tissues such as cementum, periodontal ligament and alveolar bone by stimulating normal development of these tissues, it is used to treat deep intraosseus defects.

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