scholarly journals Validation of a commercial human ELISA to measure hyaluronic acid concentration in feline plasma

2021 ◽  
pp. 104063872110447
Author(s):  
Kaela E. Shaw ◽  
Alexa M. Bersenas ◽  
Shane W. Bateman ◽  
Shauna L. Blois ◽  
R. Darren Wood
Keyword(s):  
Hyaluronic Acid ◽  
Clinical Research ◽  
Critically Ill ◽  
Acid Concentration ◽  
Extraction Efficiency ◽  
Analytical Performance ◽  
Research Setting ◽  
Assay Validation ◽  
Validation Methods ◽  
Precision Study

Our goal was to validate a human hyaluronic acid (HA) ELISA (Hyaluronic acid plus ELISA; TECOmedical Group) for use in feline plasma. Plasma from 5 healthy cats and 5 critically ill cats was used for validation of the assay. Validation methods performed included intra- and inter-assay variability, spike-and-recovery, and dilutional linearity. All measurements were performed in duplicate. The precision study revealed good intra-assay CV of 7.4–8.9%; inter-assay CV was 3.4–4.2%. Extraction efficiency via spiking tests yielded mean recovery of 89.6%. The assay met criteria for acceptable linearity using 3 serial dilutions. Our results demonstrate that this commercial HA ELISA had acceptable analytical performance using feline plasma and could be a useful tool in the veterinary clinical research setting.

Hyaluronic acid filler vascular complication management: an updated and easy-to-follow emergency protocol

2021 ◽  
Vol 10 (Sup1) ◽  
pp. 34-38
Author(s):  
Erick Santaella-Sosa
Keyword(s):  
Hyaluronic Acid ◽  
Acid Concentration ◽  
Current Literature ◽  
Vascular Complication ◽  
Complication Management ◽  
Biophysical Properties ◽  
Product Characteristics ◽  
Physiochemical Properties ◽  
Molecular Weights

With the breadth and variety of hyaluronic acid hydrogels available, it can be challenging to understand the evolving product characteristics and associated terminology. Similarly, different hyaluronic acid hydrogels can share the same indication, and yet consist of different rheological and physiochemical properties. In this paper, hyaluronic acid biophysical properties, such as molecular weights, stabilisation (crosslinking), modification and hyaluronic acid concentration, are explored in relation to findings from current literature. The significance for these specific properties is explored in relation to specific indications and anti-ageing benefits.

scholarly journals Analysis of hyaluronic acid concentration in rat vocal folds during estral and gravidic puerperal cycles

2009 ◽  
Vol 75 (5) ◽  
pp. 654-659
Author(s):  
José Eduardo de Sá Pedroso ◽  
Osíris Camponês do Brasil ◽  
João Roberto Maciel Martins ◽  
Helena Bociane Nader ◽  
Manuel de Jesus Simões
Keyword(s):  
Hyaluronic Acid ◽  
Acid Concentration ◽  
Vocal Folds

In vitro synthesis of glycosaminoglycans in endocrine ophthalmopathy

1992 ◽  
Vol 127 (5) ◽  
pp. 397-402 ◽  
Author(s):  
G Kahaly ◽  
C Stover ◽  
J Beyer ◽  
E Otto
Keyword(s):  
Hyaluronic Acid ◽  
Acid Concentration ◽  
Cetylpyridinium Chloride ◽  
Acid Synthesis ◽  
Cell Mediated Immunity ◽  
Glycosaminoglycan Synthesis ◽  
In Vitro Synthesis ◽  
Significant Difference ◽  
Endocrine Ophthalmopathy

The effects of humoral and cell-mediated immunity on the glycosaminoglycan synthesis of retrobulbar fibroblasts was evaluated in patients with endocrine ophthalmopathy. After incubation with IgG and sera, secreted glycosaminoglycans, radiolabeled with D-6-3H-glucosamine and 35sulfate, were precipitated with cetylpyridinium chloride and ethanol. Hyaluronic acid synthesis of human retrobulbar fibroblasts after incubation with sera and IgG and after co-culture with lymphocytes was assessed by means of a radiometric test. Patients' IgG, compared to controls', accounted for a higher secretory stimulation of porcine retrobulbar fibroblasts (as measured by cetylpyridinium chloride precipitation) after 24 and 48 h. Contrasting with 24 h incubation time, glycosaminoglycan values after 48 h were increased two to threefold. Patients' and controls' sera caused earlier and stronger, yet indistinguishable glycosaminoglycan production. Non-sulfated hyaluronic acid was the preponderant glycosaminoglycan secreted into the media by retrobulbar fibroblasts. As assessed with the radiometric test, incubation with patients' and controls' sera and IgG did not reveal a significant difference in stimulating the hyaluronic synthesis of patients' and controls' retrobulbar fibroblasts. When measuring the hyaluronic acid synthesis of controls' and patients' retrobulbar fibroblasts after co-cultivation of lymphocytes, however, patients' lymphocytes had a marked ability to increase the hyaluronic acid concentration compared to controls' lymphocytes. The hyaluronic acid concentration after incubation of a patient's retrobulbar fibroblasts with autologous lymphocytes was markedly more elevated than the intrinsic hyaluronic acid production of retrobulbar fibroblasts. In conclusion, though a significant in vitro influence of patients' IgG and sera on the glycosaminoglycan release of both porcine and human (patients' as well as controls') retrobulbar fibroblasts could not be observed in this study, the indications of a marked stimulatory influence of lymphocytes on the hyaluronic acid secretion of retrobulbar fibroblasts demand further investigation.

Organic acid mediated nutrient extraction efficiency in three calcareous soils

Soil Research ◽  
2009 ◽  
Vol 47 (2) ◽  
pp. 213 ◽  
Author(s):  
Z. Khademi ◽  
D. L. Jones ◽  
M. J. Malakouti ◽  
F. Asadi ◽  
M. Ardebili
Keyword(s):  
Organic Acids ◽  
Organic Acid ◽  
Acid Concentration ◽  
Extraction Efficiency ◽  
Solid Phase ◽  
Calcareous Soils ◽  
Ionic Form ◽  
High Concentrations ◽  
Extractant Solution ◽  
Nutrient Extraction

This study was conducted to evaluate the effects of organic acids on the concentrations of metals and anions in soil solution. Three soils with contrasting CaCO3 contents were extracted with organic acid solutions (citrate and oxalate) of different concentrations for different time periods and analysed for Fe, Mn, Zn, Cu, P, and Ca. The soils showed a significant change after the addition of the organic acids to the soil. The mobilisation of metals from the solid phase was dependent on concentration and ionic form of organic acid. High concentrations of citric acid were more effective than oxalate in mobilising Ca, Fe, Mn, and Zn. Overall; oxalate was slightly more effective than citrate in mobilising P. Generally, the higher the organic acid concentration of the extractant solution, the greater was the amount of elements extracted from the soil. Citrate tended to be more effective than oxalate at mobilising elements from the soil. All pH changes were dependent on organic acid concentration.

Barriers and Facilitators to Obtaining Informed Consent in a Critical Care Pediatric Research Ward in Southern Malawi

2019 ◽  
Vol 14 (2) ◽  
pp. 152-168
Author(s):  
Lucinda Manda-Taylor ◽  
Alice Liomba ◽  
Terrie E. Taylor ◽  
Kristan Elwell
Keyword(s):  
Critical Care ◽  
Informed Consent ◽  
Clinical Research ◽  
Critically Ill ◽  
Health Care Providers ◽  
Pediatric Intensive Care ◽  
Research Participant ◽  
Critically Ill Children ◽  
Parental Distress ◽  
Care Providers

Informed consent is an ethical requirement in clinical research. Obtaining informed consent is challenging in resource-constrained settings. We report results of a formative qualitative study that examined factors that facilitate and hinder informed consent for clinical research among critically ill children in Malawi. We argue that truly informed consent in a pediatric intensive care unit (PICU) is challenged by parental distress, time constraints when balancing care for critically ill patients with research-related tasks, and social hierarchies and community mistrust toward certain research procedures. We interviewed health care providers and parents of children attending a critical care unit to identify potential challenges and solicit strategies for addressing them. Providers and caregivers suggested practical solutions to enhance research participant understanding of clinical trial research, including the use of visual materials, community engagement strategies, and using patients as advocates in promoting understanding of research procedures.

Hyaluronic acid concentration of diabetic rats’ vaginas treated with soybean isoflavones or 17β-estradiol

Maturitas ◽  
2015 ◽  
Vol 82 (3) ◽  
pp. 318
Author(s):  
A.A.F. Carbonel ◽  
C.R.A. Bertoncini ◽  
V.C. do Amaral ◽  
P.L. da Silva ◽  
R.S. Simoes
Keyword(s):  
Hyaluronic Acid ◽  
Acid Concentration ◽  
Diabetic Rats ◽  
Soybean Isoflavones ◽  
17Β Estradiol
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