Cloning, Expression, and Sequence Analysis of the ORF4 Gene of the Porcine Reproductive and Respiratory Syndrome Virus MN-1b
1994 ◽
Vol 6
(3)
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pp. 293-296
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Keyword(s):
Porcine reproductive and respiratory syndrome virus (PRRSV) MN-1b strain open reading frame 4 (ORF4) has been cloned, sequenced, and expressed in Escherichia coli. The homologies of nucleotide and amino acid sequences between MN-1b (US isolate) and LV (European isolate) are 69% and 64%, respectively. The data also showed that ORF4 of MN-1b is 36 bases shorter than that of LV. Western blot analysis of expressed recombinant ORF4 protein reacted with 65% (26/40) of PRRSV-infected pig sera tested. These results demonstrated that ORF4 of PRRSV may not be a well-conserved region.
2000 ◽
Vol 68
(5)
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pp. 2720-2727
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Keyword(s):
2000 ◽
Vol 182
(21)
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pp. 6243-6246
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Keyword(s):
1998 ◽
Vol 42
(2)
◽
pp. 436-439
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Keyword(s):
2004 ◽
Vol 70
(3)
◽
pp. 1570-1575
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1986 ◽
Vol 6
(5)
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pp. 1711-1721
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Keyword(s):