Genetic variation and phylogenetic relationships of 22 porcine reproductive and respiratory syndrome virus (PRRSV) field strains based on sequence analysis of open reading frame 5

1997 ◽  
Vol 142 (5) ◽  
pp. 993-1001 ◽  
Author(s):  
V. G. Andreyev ◽  
R. D. Wesley ◽  
W. L. Mengeling ◽  
A. C. Vorwald ◽  
K. M. Lager
Keyword(s):  
Genetic Variation ◽  
Sequence Analysis ◽  
Phylogenetic Relationships ◽  
Open Reading Frame ◽  
Respiratory Syndrome Virus ◽  
Reading Frame

Genetic variation in open reading frame 5 gene of porcine reproductive and respiratory syndrome virus in Taiwan

2008 ◽  
Vol 131 (3-4) ◽  
pp. 339-347 ◽  
Author(s):  
Chun Wang ◽  
Fan Lee ◽  
Tien-Shine Huang ◽  
Chu-Hsiang Pan ◽  
Ming-Hwa Jong ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Open Reading Frame ◽  
Respiratory Syndrome Virus ◽  
Reading Frame

scholarly journals Cloning, Expression, and Sequence Analysis of the ORF4 Gene of the Porcine Reproductive and Respiratory Syndrome Virus MN-1b

1994 ◽  
Vol 6 (3) ◽  
pp. 293-296 ◽  
Author(s):  
Jimmy Kwang ◽  
Hyun Soo Kim ◽  
Han S. Joo
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Sequence Analysis ◽  
Amino Acid Sequences ◽  
Open Reading Frame ◽  
Respiratory Syndrome Virus ◽  
European Isolate ◽  
Reading Frame ◽  
Orf4 Protein ◽  
Orf4 Gene

Porcine reproductive and respiratory syndrome virus (PRRSV) MN-1b strain open reading frame 4 (ORF4) has been cloned, sequenced, and expressed in Escherichia coli. The homologies of nucleotide and amino acid sequences between MN-1b (US isolate) and LV (European isolate) are 69% and 64%, respectively. The data also showed that ORF4 of MN-1b is 36 bases shorter than that of LV. Western blot analysis of expressed recombinant ORF4 protein reacted with 65% (26/40) of PRRSV-infected pig sera tested. These results demonstrated that ORF4 of PRRSV may not be a well-conserved region.

scholarly journals Open reading frame 132 of Heliocoverpa armigera nucleopolyhedrovirus encodes a functional per os infectivity factor (PIF-2)

2006 ◽  
Vol 87 (9) ◽  
pp. 2563-2569 ◽  
Author(s):  
Minggang Fang ◽  
Yingchao Nie ◽  
Qian Wang ◽  
Fei Deng ◽  
Ranran Wang ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Western Blot ◽  
Blot Analysis ◽  
Post Infection ◽  
Spodoptera Exigua ◽  
Open Reading Frame ◽  
Rt Pcr ◽  
Western Blots ◽  
Reading Frame ◽  
Budded Virus

Open reading frame 132 (Ha132) of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) is a homologue of per os infectivity factor 2 (pif-2) of Spodoptera exigua multiple nucleopolyhedrovirus. Sequence analysis indicated that Ha132 encoded a protein of 383 aa with a predicted molecular mass of 44.5 kDa. Alignment of HA132 and its baculovirus homologues revealed that HA132 was highly conserved among baculoviruses, with 14 absolutely conserved cysteine residues. RT-PCR indicated that Ha132 was first transcribed at 24 h post-infection. Western blot analysis showed that a 43 kDa band was detectable in HearNPV-infected HzAM1 cells from 36 h post-infection. Western blots also indicated that HA132 was a component of the occlusion-derived virus, but not of budded virus. Deletion of Ha132 from HearNPV abolished per os infectivity, but had no effect on the infectivity of the budded virus phenotype.

Open reading frame 5 of porcine reproductive and respiratory syndrome virus as a cause of virus-induced apoptosis.

1996 ◽  
Vol 70 (5) ◽  
pp. 2876-2882 ◽  
Author(s):  
P Suárez ◽  
M Díaz-Guerra ◽  
C Prieto ◽  
M Esteban ◽  
J M Castro ◽  
...  
Keyword(s):  
Open Reading Frame ◽  
Respiratory Syndrome Virus ◽  
Reading Frame ◽  
Induced Apoptosis

scholarly journals Outbreak of equid herpesvirus 1 abortions at the Arabian stud in Poland

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Karol Stasiak ◽  
Magdalena Dunowska ◽  
Jerzy Rola
Keyword(s):  
Amino Acid ◽  
Sequence Analysis ◽  
Amino Acid Sequence ◽  
Neurological Disease ◽  
Open Reading Frame ◽  
Case Presentation ◽  
Reading Frame ◽  
Equid Herpesvirus ◽  
Herpesvirus 1 ◽  
Horizontal Spread

Abstract Background Equid herpesvirus 1 (EHV-1) infections are endemic worldwide, including Poland. Many are subclinical, but some are associated with respiratory disease, abortion, neonatal foal death, or neurological disease. We describe an outbreak of abortions in Arabian mares at a well-managed State stud farm in Poland. Case presentation Eight of 30 pregnant mares aborted and one gave birth to a weak foal that died within 72 h after birth. EHV-1 was isolated from all fetuses as well as from the diseased foal. All viruses belonged to the N752 variant based on the predicted open reading frame (ORF) 30 amino acid sequence. All were identical to each other and to previous EHV-1 viruses from the same stud based on the ORF68 sequence analysis. The outbreak coincided with the lapse in the routine yearly EHV-1/4 vaccinations of the mares. Conclusions Multiple abortion due to EHV-1 infection can occur in well-managed groups of horses. Reactivation of latent EHV-1 in one of the resident mares followed by a horizontal spread was considered the most likely explanation for the outbreak. Routine vaccination is an important part of a herd-heath program.

scholarly journals Glycine Betaine Transmethylase Mutant of Pseudomonas aeruginosa

2002 ◽  
Vol 184 (15) ◽  
pp. 4301-4303 ◽  
Author(s):  
Ana L. Serra ◽  
Javier F. Mariscotti ◽  
José L. Barra ◽  
Gloria I. Lucchesi ◽  
Carlos E. Domenech ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Sequence Analysis ◽  
Glycine Betaine ◽  
Nitrogen Sources ◽  
Open Reading Frame ◽  
Carbon And Nitrogen Sources ◽  
Reading Frame ◽  
Carbon And Nitrogen ◽  
Molecular Approaches ◽  
Knockout Gene

ABSTRACT The gene for glycine betaine transmethylase (gbt) was identified in Pseudomonas aeruginosa strain Fildes III by biochemical, physiological, and molecular approaches. Based on sequence analysis, the knockout gene corresponded to an open reading frame (ORF) named PA3082 in the genome of P. aeruginosa PAO1. The translated product of this ORF displayed similarity to transferases of different microorganisms. Mutation in gbt blocked the utilization of choline and glycine betaine as carbon and nitrogen sources.

scholarly journals Knot Formation Caused by Pseudomonas syringae subsp. savastanoi on Olive Plants Is hrp-Dependent

2004 ◽  
Vol 94 (5) ◽  
pp. 484-489 ◽  
Author(s):  
A. Sisto ◽  
M. G. Cipriani ◽  
M. Morea
Keyword(s):  
Sequence Analysis ◽  
Pseudomonas Syringae ◽  
Open Reading Frame ◽  
Gene Encoding ◽  
Disease Symptoms ◽  
Phytopathogenic Bacteria ◽  
Reading Frame ◽  
Nonhost Plant ◽  
Induced Mutant

The virulence of Pseudomonas syringae subsp. savastanoi, which causes hyperplastic symptoms (knots) on olive plants, is associated with secreted phytohormones. We identified a Tn5-induced mutant of P. syringae subsp. savastanoi that did not cause disease symptoms on olive plants although it was still able to produce phytohormones. In addition, the mutant failed to elicit a hypersensitive response in a nonhost plant. Molecular characterization of the mutant revealed that a single Tn5 insertion occurred within an open reading frame encoding a protein 92% identical to the HrcC protein of P. syringae pv. syringae. Moreover, sequence analysis revealed that the gene encoding the HrcC protein in P. syringae subsp. savastanoi was part of an operon that included five genes arranged as in other phytopathogenic bacteria. These results imply that hrp/hrc genes are functional in P. syringae subsp. savastanoi and that they play a key role in the pathogenicity of this plant pathogen.

scholarly journals Phylogenetic relationships among sandfly fever group viruses (Phlebovirus: Bunyaviridae) based on the small genome segment

2007 ◽  
Vol 88 (8) ◽  
pp. 2312-2319 ◽  
Author(s):  
Fangling Xu ◽  
Hongli Chen ◽  
Amelia P. A. Travassos da Rosa ◽  
Robert B. Tesh ◽  
Shu-Yuan Xiao
Keyword(s):  
Genetic Divergence ◽  
Phylogenetic Relationships ◽  
Phylogenetic Analyses ◽  
Genome Segment ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Small Genome ◽  
Sequence Identity ◽  
Nucleoprotein N ◽  
Antigenic Relationships

The phleboviruses are more diverse in terms of arthropod vectors and antigenic relationships than most other genera of arthropod-borne viruses. In this study, 30 sandfly fever group viruses from the Naples, Sicilian, Punta Toro, Icoaraci and Frijoles serocomplexes were sequenced. Phylogenetic analyses were performed based on the sequence of the open reading frame for the nucleoprotein (N) and non-structural (NSs) protein genes of the small (S) segment. The five resultant genotypic lineages correlated with the serological grouping and were similar to analysis of M segment sequences. The sequence identity for N and NSs genes within the Sicilian, Naples, Punta Toro, Icoaraci and Frijoles serocomplexes was determined. The results indicated that genetic divergence for the S segment is lower than that for the M segment, suggesting that the S segment is more stable during evolution.

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