scholarly journals The Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure (InterGEN) Study

2016 ◽  
Vol 18 (5) ◽  
pp. 521-530 ◽  
Author(s):  
Jacquelyn Y. Taylor ◽  
Michelle L. Wright ◽  
Cindy A. Crusto ◽  
Yan V. Sun
Keyword(s):  
Blood Pressure ◽  
Psychological Factors ◽  
Saliva Sample ◽  
Design Procedure ◽  
Interaction Effects ◽  
Sample Collection ◽  
Weight Percentage ◽  
False Discovery ◽  
Gene Environment ◽  
Collection Tube

The Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure (InterGEN) study aims to delineate the independent and interaction effects of genomic (genetic and epigenetic) and psychological–environmental (maternally perceived racial discrimination, mental health, and parenting behavior) factors on blood pressure (BP) among African American mother–child dyads over time. The purpose of this article is to describe the two-step genetic and epigenetic approach that will be executed to explore Gene × Environment interactions on BP using a longitudinal cohort design. Procedure for the single collection of DNA at Time 1 includes the use of the Oragene 500-format saliva sample collection tube, which provides enough DNA for both the Illumina Multi-Ethnic Genotyping and 850K EPIC methylation analyses. BP readings, height, weight, percentage of body fat, and percentage of body water will be measured on all participants every 6 months for 2 years for a total of 4 time points. Genomic data analyses to be completed include multivariate modeling, assessment of population admixture and structure, and extended analyses including Bonferroni correction, false discovery rate methods, Monte Carlo approach, EIGENSTRAT methods, and so on, to determine relationships among both main and interaction effects of genetic, epigenetic, and psychological environmental factors on BP.

scholarly journals 0407 Sleep, Stress, Cardiovascular, and Psychological Health in College Students

SLEEP ◽  
2020 ◽  
Vol 43 (Supplement_1) ◽  
pp. A156-A156
Author(s):  
A M Reynolds ◽  
K M Mullins
Keyword(s):  
College Students ◽  
Blood Pressure ◽  
Heart Rate ◽  
Psychological Health ◽  
Psychological Factors ◽  
Cardiovascular Health ◽  
Sleep Efficiency ◽  
Poor Sleep ◽  
Sleep Habits ◽  
Stress Symptoms

Abstract Introduction Epidemiological studies have long established that sleep factors, stress, and cardiovascular health are related. College students often struggle with the demands of college life, which leads to increased stress, symptoms of depression and anxiety, and poor sleep. The focus of the current study was to examine habitual sleep habits in college students, in association with psychological factors and physiological factors. Methods Participants included 51 undergraduate students (18 men, average age M=20.25 years, SD=1.78) who wore wrist actigraphs to measure their typical sleep habits. After one week, participants completed questionnaires about psychological symptoms (i.e., depression, anxiety, and stress; Depression Anxiety Stress Scale, DASS-21) and subjective physiological symptoms (i.e., fatigue; Multidimensional Assessment of Fatigue Scale, MAF). Blood pressure and heart rate were measured using a wrist cuff. Results Overall total sleep time was 6.59 hours and sleep efficiency was 82.55%. Pearson correlational analyses revealed a negative moderate association between sleep efficiency and diastolic blood pressure (r(49) = -.318, p = .024). Global PSQI scores were moderately associated with stress (r(49) = .419, p = .002). MAF Global Fatigue Index scores revealed positive associations with depression (r(49) = .344, p =.014), anxiety (r(49) = .474, p<.001), and stress (r(49) = .620 p<.001). Heart rate was positively associated with depressive symptoms r(49) = .296, p= .035), stress symptoms r(49) = .447, p= .001), and fatigue r(49) = .456, p= .001). Conclusion As expected, college students’ sleep was short in duration and poor in efficiency. Sleep factors, cardiovascular factors, psychological factors, and stress were all related, demonstrating the importance of sleep on physiological and psychological health. More research should be conducted to further examine the relationships and directionality between sleep, psychological factors, and stress as there may be underlying mechanisms important for cardiovascular health. Support None.

scholarly journals Prevalence of metabolic components in university students

2014 ◽  
Vol 22 (6) ◽  
pp. 1041-1047 ◽  
Author(s):  
Ana Roberta Vilarouca da Silva ◽  
Luana Savana Nascimento de Sousa ◽  
Telma de Sousa Rocha ◽  
Ramiro Marx Alves Cortez ◽  
Layla Gonçalves do Nascimento Macêdo ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Metabolic Syndrome ◽  
Fasting Blood Glucose ◽  
Significant Proportion ◽  
Hdl Cholesterol ◽  
Clinical Analysis ◽  
Abdominal Circumference ◽  
Sample Collection ◽  
Glucose Levels ◽  
Low Hdl

OBJECTIVE: to identify the frequency of components of Metabolic Syndrome (MetS) among university students.METHOD: descriptive study with 550 students, from various courses run by a public university. The socioeconomic data, lifestyle, and components of MetS were filled out using a questionnaire. Blood sample collection was undertaken in the university itself by a contracted clinical analysis laboratory.RESULTS: 66.2% were female, with a mean age of 22.6±4.41; 71.7% were sedentary; 1.8% stated that they smoke; and 48.5% were classified as at medium risk for alcoholism. 5.8% had raised abdominal circumference and 20.4% had excess weight; 1.3% and 18.9% had raised fasting blood glucose levels and triglycerides, respectively; 64.5% had low HDL cholesterol and 8.7% had blood pressure levels compatible with borderline high blood pressure. Thus, of the sample, 64.4% had at least one component for MetS; 11.6% had two, and 3.5% had three or more.CONCLUSION: a significant proportion of the population already has the components for metabolic syndrome, and this profile reinforces the importance of early diagnosis so as to reduce the risk of developing chronic comorbidities.

Does Casein Phosphopeptid Amorphous Calcium Phosphate Provide Remineralization on White Spot Lesions and Inhibition of Streptococcus mutans?

2014 ◽  
Vol 38 (4) ◽  
pp. 302-306 ◽  
Author(s):  
Arzu Aykut-Yetkiner ◽  
Nazan Kara ◽  
Mustafa Ateş ◽  
Nazan Ersin ◽  
Fahinur Ertuğrul
Keyword(s):  
Calcium Phosphate ◽  
Streptococcus Mutans ◽  
Amorphous Calcium Phosphate ◽  
Saliva Sample ◽  
Test Group ◽  
White Spot ◽  
Control Group ◽  
White Spot Lesions ◽  
Sample Collection ◽  
Significant Difference

Objective: The aim of this study was to evaluate the remineralization effect of Casein Phosphopeptid Amorphous Calcium Phosphate (CPP-ACP) on white spot lesions (WSL) and its inhibitory effect on Streptococcus mutans colonization. Study design: The study group consisted of 60 children exhibiting at least 1-WSL. Subjects were randomly divided into 2 groups: a test group of using CPP-ACP cream (Tooth Mousse, GC Europe N.V., Leuven, Belgium) and a control group using only fluoride containing toothpaste for a period of 3-months. Baseline WSLs were scored using DIAGNOdent device (KaVo Germany) and the saliva samples were collected to measure S. mutans counts. After the 3-month period the WSLs were again recorded and the saliva sample collection was repeated. Wilcoxon Signed Ranks Test was used for statistical analysis. Results: DIAGNOdent measurements were increased by time (p=0.002) in control group and no statistically significant difference (p=0.217) was found in test group by the 3-month period. In both groups, the mutans counts were decreased in 3-month experimental period. Conclusions: These clinical and laboratory results suggested that CPP-ACP containing cream had a slight remineralization effect on the WSL in the 3-month evaluation period however longer observation is recommended to confirm whether the greater change in WSLs is maintained.

Application of the Model of Allostasis to Older Women’s Relocation to Senior Housing

2013 ◽  
Vol 16 (2) ◽  
pp. 197-208 ◽  
Author(s):  
Heidi H. Ewen ◽  
Jennifer Kinney
Keyword(s):  
Older Women ◽  
Allostatic Load ◽  
Stress Reactivity ◽  
Saliva Sample ◽  
Well Being ◽  
Lifestyle Changes ◽  
Sample Collection ◽  
Senior Housing ◽  
Detailed Picture ◽  
Analyze Data

Objectives:Adjustment to senior housing entails significant lifestyle changes and is a stressful process. The adaptation process is dynamic and has yet to be studied using the conceptual model of allostasis. This article presents exemplars of women whose profiles represent three allostatic states: successful adaptation (homeostasis), ongoing adaptation (allostasis), and maladaptation (allostatic load).Method:Older women who had relocated to senior housing participated in three interviews and monthly saliva sample collection over a 6-month period. Saliva was assayed for diurnal cortisol secretion. Triangulation of mixed methods was used to analyze data, and psychosocial data were mapped onto the cortisol graphs to illustrate changes in stress reactivity and well-being.Results:Coping abilities, perceptions of stressors, and cortisol measures provide a detailed picture of the interplay among events and perceptions and the effects of both on well-being.Discussion:The case exemplars provide detailed information on the complexity of psychosocial and physiological components of the model of allostasis. This study also fills a gap in knowledge on negative relocation outcomes using the allostatic model.

Effects of Gene-Environment Interactions on Cardiovascular Risk Factors in Chinese Adolescent Twins

1984 ◽  
Vol 33 (3) ◽  
pp. 383-396
Author(s):  
C.J. Chen ◽  
B.H. Cohen ◽  
E.L. Diamond ◽  
T.M. Lin ◽  
J.S. Chen
Keyword(s):  
Blood Pressure ◽  
Serum Cholesterol ◽  
Population Based ◽  
Serum Triglycerides ◽  
Gene Environment ◽  
Chinese Adolescent ◽  
Environmental Agents ◽  
Animal Organ ◽  
Adolescent Twins ◽  
The Impact

AbstractA population-based sample of 73 male and 77 female monozygotic (MZ), and 41 male and 33 female dizygotic (DZ) Chinese adolescent twin pairs were studied to assess effects of gene-environment interactions of systolic blood pressure (SBP), diastolic blood pressure (DBP), serum cholesterol and triglyceride levels. Intrapair concordance in BP levels was found to be significantly associated with the interaction of zygosity and salty foods preference and also with that of zygosity and vegetable preference. A consistently positive and statistically significant association was observed between the intrapair difference in serum cholesterol and the interaction of zygosity and animal organ preference; while intrapair concordance in serum cholesterol was associated with the interaction of zygosity and milk consumption. Intrapair difference in serum triglycerides was associated with the interaction of zygosity and fish preference, and a significant association was also found between the intrapair concordance in serum triglycerides and the interaction of zygosity and sweets preference. These observations suggest that the impact of these environmental agents may be influenced by the genotype.

scholarly journals Undergoing Venipuncture in Healthcare Education: The Psycho-Biological Effect on Students

2008 ◽  
Author(s):  
Orly Sarid ◽  
Ofra Anson ◽  
Dagan Schwartz ◽  
Arich Yaari
Keyword(s):  
Health Care ◽  
Saliva Sample ◽  
Mood States ◽  
Educational Practices ◽  
Significant Implication ◽  
Sample Collection ◽  
Hsp 70 ◽  
Health Care Students ◽  
Before And After ◽  
Pencil And Paper

Venipuncture training is an integral part of healthcare educational programs. Despite its extensive use, no previous studies have evaluated the psychobiological impact of this procedure among health-care students. Participants were 26 healthy students, practicing venipuncture on each other as part of their curricular activity. Self reported pencil and paper questionnaires aimed at assessing depression, anxiety and mood states were administered before and after venipuncture and saliva sample collection. Anti heat shock protein (Hsp) 70 and cortisol levels in blood and saliva were collected from the participants. A reduction in anxiety, anger and confusion mood states took place after venipuncture. All participants had high levels of cortisol and anti-Hsp 70 in the serum and saliva. Anti-Hsp 70 and cortisol evaluated in the serum were correlated with some of the mood variables. Routine educational practices such as venipuncture elicit significant psychological stress. If confirmed by additional studies it will have a significant implication on health-care educational practices.

scholarly journals DRUL for School: Opening Pre-K with safe, simple, sensitive saliva testing for SARS-CoV-2

2021 ◽  
Author(s):  
Mayu Frank ◽  
Nathalie E Blachere ◽  
Salina Parveen ◽  
Ezgi Hacisuleyman ◽  
John Fak ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Saliva Sample ◽  
Sample Collection ◽  
Viral Transport ◽  
Children And Parents ◽  
Safety Studies ◽  
Viral Copy ◽  
Saliva Collection ◽  
Highly Correlated ◽  
University Laboratory

To address the need for simple, safe, sensitive, and scalable SARS-CoV-2 tests, we validated and implemented a PCR test that uses a saliva collection kit use at home. Individuals self-collected 300 ul saliva in vials containing Darnell Rockefeller University Laboratory (DRUL) buffer and extracted RNA was assayed by RT-PCR (the DRUL saliva assay). The limit of detection was confirmed to be 1 viral copy/ul in 20 of 20 replicate extractions. Viral RNA was stable in DRUL buffer at room temperature up to seven days after sample collection, and safety studies demonstrated that DRUL buffer immediately inactivated virus at concentrations up to 2.75x106 PFU/ml. Results from SARS-CoV-2 positive nasopharyngeal (NP) swab samples collected in viral transport media and assayed with a standard FDA Emergency Use Authorization (EUA) test were highly correlated with samples placed in DRUL buffer. Direct comparison of results from 162 individuals tested by FDA EUA oropharyngeal (OP) or NP swabs with co-collected saliva samples identified four otherwise unidentified positive cases in DRUL buffer. Over six months, we collected 3,724 samples from individuals ranging from 3 months to 92 years of age. This included collecting weekly samples over 10 weeks from teachers, children, and parents from a pre-school program, which allowed its safe reopening while at-risk pods were quarantined. In sum, we validated a simple, sensitive, stable, and safe PCR-based test using a self-collected saliva sample as a valuable tool for clinical diagnosis and screening at workplaces and schools.

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