Radioprotective Effect ofAchillea millefoliumL Against Genotoxicity Induced by Ionizing Radiation in Human Normal Lymphocytes

Objectives: Gliclazide (GL) is widely used to reduce hyperglycemia in diabetic patients. The aim of this study was to investigate the protective effect of GL against chromosome damage induced by ionizing radiation in human blood lymphocytes. Methods: In this experimental study, peripheral blood samples were collected from human volunteers and treated with GL at various concentrations (5, 25, 50 or 100 μM) for three hours. Then samples were irradiated to X-ray (1.5 Gy). Blood samples were cultured with mitogenic stimulation. The frequencies of micronuclei in cytokinesis-blocked binucleated lymphocytes were determined in the different samples. The antioxidant activities of GL were assayed by two different methods as 1,1- diphenyl-2-picryl hydrazyl radical (DPPH) free radical scavenging and reducing antioxidant power assays. Results: GL significantly reduced the percentage of micronuclei in lymphocytes which were irradiated. The maximum radioprotection in the reduction of percentage of micronuclei in lymphocytes was observed at 100 μM of GL with 52% efficacy. GL exhibited excellent free radical scavenging activity and reducing power at concentration dependent activities. The IC50 values of GL were lower than ascorbic acid. Higher potencies were observed in the antioxidant activities for GL than ascorbic acid in both methods. Conclusion: This data exhibits that GL is a powerful radioprotective agent that could protect healthy cells against the chromosome damage induced by ionizing radiation through antioxidant activity. The radioprotective effect is new indication of GL for patients' protection against side effect induced by ionizing radiation.

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Radioprotective effect of atorvastatin against ionizing radiation-induced nephrotoxicity in mice

International Journal of Radiation Biology ◽

10.1080/09553002.2018.1420926 ◽

2018 ◽

Vol 94 (2) ◽

pp. 106-113 ◽

Cited By ~ 9

Author(s):

Fereshteh Talebpour Amiri ◽

Maedeh Hamzeh ◽

Ramezan Ali Naeimi ◽

Arash Ghasemi ◽

Seyed Jalal Hosseinimehr

Keyword(s):

Ionizing Radiation ◽

Radioprotective Effect ◽

Radiation Induced

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Radioprotective Effect of Cerium Oxide Nanoparticles Against Genotoxicity Induced by Ionizing Radiation on Human Lymphocytes

Current Radiopharmaceuticals ◽

10.2174/1874471011666180528095203 ◽

2018 ◽

Vol 11 (2) ◽

pp. 109-115 ◽

Cited By ~ 7

Author(s):

Zohreh Zal ◽

Arash Ghasemi ◽

Shima Azizi ◽

Hossein Asgarian-Omran ◽

Azadeh Montazeri ◽

...

Keyword(s):

Ionizing Radiation ◽

Cerium Oxide ◽

Human Lymphocytes ◽

Radioprotective Effect ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles

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Morphological Aspects of Radioprotection in the Experiment

Journal of New Medical Technologies ◽

10.12737/5008 ◽

2014 ◽

Vol 21 (2) ◽

pp. 90-94

Author(s):

Золотарева ◽

S. Zolotareva ◽

Воронцова ◽

Zoya Vorontsova ◽

Логачева ◽

...

Keyword(s):

Ionizing Radiation ◽

Electromagnetic Radiation ◽

Pathological Process ◽

Radioprotective Effect ◽

Low Oxygen ◽

Normobaric Oxygen ◽

Emergency Situations ◽

Morphological Aspects ◽

Functional Reserves ◽

Overall Resistance

The problem of modification of the effects of ionizing radiation in morphological manifestations of authorities at different speeds update is relevant direction in recent years. The authors considered as modifiers - hypoxic gas mixture, normobaric oxygen and electromagnetic radiation. They are in certain conditions associated with ionizing radiation, as in the conditions of emergency situations, and in normal mode. The development of pathological process after exposure and outcome depend on the overall resistance and state systems of adaptation. Identify the most effective mechanisms of mobilization of protective forces and the restoration of the functional reserves of the organism as a whole, is a prospective task of modern radiobiology. Based on the study of literature data and the results of their research the authors concluded that the degree of modification was dependent on the sequence of application of modifiers and doses of ionizing radiation; the radioprotective effect was in direct correlation with tissue facilities body. The radioprotective effect was revealed by during previous combined and joint use of modified gas environment with high and low oxygen content and electromagnetic radiation. The authors believe that the use of these modifiers is promising in the field of safety.

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Potential Radioprotective Effect of AT1 Receptor Antagonists Against Morphological and Ultrastructural Changes in the Testes Induced by Ionizing Radiation

International Journal of Morphology ◽

10.4067/s0717-95022017000300005 ◽

2017 ◽

Vol 35 (3) ◽

pp. 820-830 ◽

Cited By ~ 1

Author(s):

Franciele Cristina Schwarz ◽

Naira da Silva Mansano ◽

Agnaldo Bruno Chies ◽

Gustavo Arruda Viani ◽

Maria Angélica Spadella

Keyword(s):

Ionizing Radiation ◽

At1 Receptor ◽

Radioprotective Effect ◽

Ultrastructural Changes ◽

Receptor Antagonists ◽

At1 Receptor Antagonists

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Radioprotective effect of olanzapine as an anti-psychotic drug against genotoxicity and apoptosis induced by ionizing radiation on human lymphocytes

Molecular Biology Reports ◽

10.1007/s11033-019-05024-x ◽

2019 ◽

Vol 46 (6) ◽

pp. 5909-5917

Author(s):

Mohammad Asghari ◽

Zahra Shaghaghi ◽

Soghra Farzipour ◽

Arash Ghasemi ◽

Seyed Jalal Hosseinimehr

Keyword(s):

Ionizing Radiation ◽

Human Lymphocytes ◽

Radioprotective Effect ◽

Anti Psychotic

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Radioprotective Effect of Sinapic Acid Against Genotoxicity and Apoptosis Induced by Ionizing Radiation on Human Lymphocytes

Letters in Drug Design & Discovery ◽

10.2174/1570180815666180219163626 ◽

2018 ◽

Vol 15 (11) ◽

pp. 1147-1154 ◽

Cited By ~ 1

Author(s):

Fatemeh Alizadeh ◽

Mohammad Asghari ◽

Seyedeh Atekeh Torabizadeh ◽

Najmeh Rahmanian ◽

Arash Ghasemi ◽

...

Keyword(s):

Ionizing Radiation ◽

Human Lymphocytes ◽

Sinapic Acid ◽

Radioprotective Effect

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The Protective Effect of N-Acetylcysteine on Ionizing Radiation Induced Ovarian Failure and Loss of Ovarian Reserve in Female Mouse

BioMed Research International ◽

10.1155/2017/4176170 ◽

2017 ◽

Vol 2017 ◽

pp. 1-11 ◽

Cited By ~ 6

Author(s):

Wei Gao ◽

Jin-Xiao Liang ◽

Chi Ma ◽

Jing-yin Dong ◽

Qiu Yan

Keyword(s):

Oxidative Stress ◽

Ionizing Radiation ◽

Granulosa Cells ◽

Ovarian Reserve ◽

Embryo Implantation ◽

Ovarian Failure ◽

Radioprotective Effect ◽

Hormone Levels ◽

X Irradiation ◽

Radiation Induced

Ionizing radiation may cause irreversible ovarian failure, which, therefore, calls for an effective radioprotective reagent. The aim of the present study was to evaluate the potential radioprotective effect of N-acetylcysteine (NAC) on ionizing radiation induced ovarian failure and loss of ovarian reserve in mice. Kun-Ming mice were either exposed to X-irradiation (4 Gy), once, and/or treated with NAC (300 mg/kg), once daily for 7 days before X-irradiation. We examined the serum circulating hormone levels and the development of ovarian follicles as well as apoptosis, cell proliferation, and oxidative stress 24 hours after X-irradiation. In addition, morphological observations on the endometrial luminal epithelium and the fertility assessment were performed. We found that NAC successfully restored the ovarian and uterine function, enhanced the embryo implantation, improved the follicle development, and altered the abnormal hormone levels through reducing the oxidative stress and apoptosis level in granulosa cells while promoting the proliferation of granulosa cells. In conclusion, the radioprotective effect of NAC on mice ovary from X-irradiation was assessed, and our results suggested that NAC can be a potential radioprotector which is capable of preventing the ovarian failure occurrence and restoring the ovarian reserve.

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