scholarly journals Induced Pluripotent Stem Cells Attenuate Acute Lung Injury Induced by Ischemia Reperfusion via Suppressing the High Mobility Group Box-1

Dose-Response ◽  
2020 ◽  
Vol 18 (4) ◽  
pp. 155932582096934
Author(s):  
Yijun Li ◽  
Shun Wang ◽  
Jinbo Liu ◽  
Xingyu Li ◽  
Meng Lu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Acute Lung Injury ◽  
Endothelial Cell ◽  
Lung Injury ◽  
Induced Pluripotent Stem Cells ◽  
Inflammatory Cytokines ◽  
Pluripotent Stem Cells ◽  
Ischemia Reperfusion ◽  
High Mobility ◽  
Induced Pluripotent

Pulmonary endothelial cell injury is a hallmark of acute lung injury. High-mobility group box 1 (HMGB1) can modulate the inflammatory response via endothelial cell activation and release of inflammatory molecules. Thus, we tested whether induced pluripotent stem cells (iPSCs) can alleviate ischemia/reperfusion (I/R) induced lung injury, and, if so, whether HMGB1 mediates the effect in a male C57BL/6 mouse model. Intravenously injected iPSCs into mice 2 h after I/R showed a significant attenuation of lung injury (assessed by lung mechanics, edema, and histology) 24 h after reperfusion (compared with controls), along with decreases in HMGB1, phosphorylated nuclear factor-κB, inflammatory cytokines [interleukin (IL)1β, IL6 and tumor necrosis factor-α], and the activation of endothelial cells. Furthermore, these effects of iPSCs can be mimicked by blocking HMGB1 with an inhibitor in vivo and in vitro. We conclude that iPSCs can be a potential therapy for I/R-induced lung injury. These cells may exert therapeutic effects through blocking HMGB1 and inflammatory cytokines.

Induced pluripotent stem cells alleviate lung injury from mesenteric ischemia-reperfusion

2015 ◽  
Vol 79 (4) ◽  
pp. 592-601 ◽  
Author(s):  
Chorng-Kuang How ◽  
Sen-Kuang Hou ◽  
Luen-Kui Chen ◽  
Cheng-Ming Yang ◽  
Hsien-Hao Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Lung Injury ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Mesenteric Ischemia ◽  
Ischemia Reperfusion ◽  
Induced Pluripotent

scholarly journals IV Delivery of Induced Pluripotent Stem Cells Attenuates Endotoxin-Induced Acute Lung Injury in Mice

CHEST Journal ◽  
2011 ◽  
Vol 140 (5) ◽  
pp. 1243-1253 ◽  
Author(s):  
Kuang-Yao Yang ◽  
Hsin-Chin Shih ◽  
Chorng-Kuang How ◽  
Cheng-Yu Chen ◽  
Han-Shui Hsu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

Induced pluripotent stem cells reduce neutrophil chemotaxis via activating GRK2 in endotoxin-induced acute lung injury

Respirology ◽  
2017 ◽  
Vol 22 (6) ◽  
pp. 1156-1164 ◽  
Author(s):  
Vincent Yi-Fong Su ◽  
Shih-Hwa Chiou ◽  
Chi-Shiuan Lin ◽  
Wei-Chih Chen ◽  
Wen-Kuang Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Neutrophil Chemotaxis ◽  
Induced Pluripotent

Abstract 97: Epigenetic Signatures Contribute to the Superior Endothelial Cell Identity in Human Induced Pluripotent Stem Cells Derived From Endothelial Cells

2015 ◽  
Vol 117 (suppl_1) ◽  
Author(s):  
Mingtao Zhao ◽  
Shijun Hu ◽  
Rajini Srinivasan ◽  
Fereshteh Jahaniani ◽  
Ning-Yi Shao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Endothelial Cells ◽  
Endothelial Cell ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Early Passage ◽  
Tissue Of Origin ◽  
Induced Pluripotent ◽  
Epigenetic Signatures

Human induced pluripotent stem cells (iPSCs) can be derived from multiple types of somatic cells by transient overexpression of four Yamanaka factors. Epigenetic memory of the tissue of origin is seen in early passage iPSCs, which may interfere the directed differentiation towards target lineages in disease modeling and drug discovery. Here we derived human iPSC from three types of somatic cells of the same individuals: fibroblast (FB-iPSCs), endothelial cells (EC-iPSCs) and cardiac progenitor cells (CPC-iPSCs). We then differentiated them into endothelial cells by using sequential administration of Activin, BMP4, bFGF and vEGF. EC-iPSCs show higher EC differentiation propensity and EC-specific markers (PECAM1 and NOS3) gene expression in early passage iPSCs than FB-iPSCs and CPC-iPSCs. In vivo, EC-iPSC-ECs display significantly greater revascularization capacity than those of FB-iPSCs and CPC-iPSCs when transplanted to the hindlimb ischemic mice. In addition, transplanted EC-iPSC-ECs were recovered with a higher percentage of CD31+ population and higher EC-specific markers (PECAM1, KDR and ICAM) gene expression by using single cell qPCR. In vitro, EC-iPSC-ECs exhibit better endothelial cell character maintenance along with extensive culturing and passaging. Several chromatin signatures, including H3K27ac, H3K4me1 and p300 were found highly enriched in ECs and EC-iPSCs, but not in human embryonic stem cells (ESCs). Gene ontology analysis indicates that the differentially enriched regions are primarily associated with angiogenesis and vascular development, reflecting the residual epigenetic signatures in EC-iPSCs. Finally EC-specific enhancer markers undergo dynamic changes during the process of EC fate commitment and differentiation, though the majority of them sustain conserved pattern in EC-iPSCs, CPC-iPSCs and FB-iPSCs. In conclusion, these results highlight that the residual epigenetic signatures of tissue of origin may affect lineage differentiation propensity in early-passage human iPSCs.

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