Prognostic Significance of Cytosolic pS2 Protein Content in Gastric Cancer

2001 ◽  
Vol 16 (1) ◽  
pp. 37-44 ◽  
Author(s):  
C. Suárez ◽  
F. Vizoso ◽  
J.C. Rodríguez ◽  
I. García ◽  
P. Raigoso ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Standard Error ◽  
Prognostic Significance ◽  
Biological Marker ◽  
Tumor Location ◽  
Adjacent Mucosa ◽  
Gastric Adenocarcinomas ◽  
Resectable Gastric Cancer ◽  
Clinicopathologic Parameters

pS2, a 60-amino-acid chain peptide which is the most widespread estrogen-induced RNA messenger in MCF-7 breast cancer cells, is normally detected in the epithelium of gastric mucosa. The aims of this work were to evaluate the cytosolic pS2 content and its clinical significance in gastric carcinomas. Cytosolic pS2 levels were examined by immunoradiometric methods in 108 patients with primary gastric adenocarcinomas. The mean follow-up period was 23.3 months. The cytosolic pS2 levels of the tumors ranged widely, i.e., from 0.1 to 3217 ng/mg protein. There were no significant differences in pS2 content between tumors (mean ± standard error: 137.2±31.4 ng/mg protein) and paired adjacent mucosa samples (n=84; mean ± standard error: 249.6±32.6 ng/mg protein), nor were there any significant differences in tumoral pS2 levels with respect to clinicopathologic parameters such as patient age and sex or tumor location, stage, histologic type or grade. However, the results indicated that high intratumoral pS2 levels were significantly and independently associated with an unfavorable outcome in the overall group of patients (p=0.0266) and in patients with resectable gastric cancer (p=0.003). In conclusion, pS2 may represent a useful biological marker in gastric cancer.

Prognostic Significance of Tissue-Type Plasminogen Activator (tPA) Content in Gastric Cancer and Surrounding Mucosa

2002 ◽  
Vol 17 (3) ◽  
pp. 169-176 ◽  
Author(s):  
L. Sanz ◽  
F. Vizoso ◽  
P. Vérez ◽  
M.T. Allende ◽  
M.G. Corte ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Solid Phase ◽  
Prognostic Significance ◽  
Tissue Type ◽  
Gastric Neoplasms ◽  
Clinicopathological Parameters ◽  
Adjacent Mucosa ◽  
Type Plasminogen Activator ◽  
Wide Variability ◽  
Gastric Cancer Patients

Aims We analyzed the tPA content in primary gastric carcinomas and surrounding mucosa in order to assess the relationship between tPA content, clinicopathological tumor characteristics, and estrogen and progesterone receptor content. We evaluated the prognostic value of this serine protease in gastric cancer patients. Patients and methods 122 resected gastric neoplasms and 95 adjacent mucosa samples were studied. The tPA content was measured in cytosol by an ELISA method. Cytosolic ER and PgR were measured with a solid phase enzyme immunoassay. Results Cytosolic tPA levels in neoplastic tissues (median 1.0 ng/mg prot) were significantly lower (p=0.002) than those found in paired mucosa samples (median 2.3 ng/mg prot). There was no significant association between tPA levels and clinicopathological parameters or PgR content, but tPA levels were significantly correlated with ER content. The intermediate-tPA-content group, corresponding to samples with between 0.3 and 1.70 ng/mg protein, proved to have a significantly high risk of relapse. Conclusions We found a wide variability in tPA levels in gastric carcinoma and adjacent mucosa samples, with significantly decreased levels in tumors and a significantly positive relationship between tPA levels and ER status. There was a non-monotonic relationship between tPA levels and prognosis in patients with gastric cancer.

scholarly journals Prognostic Significance of Microvessel Density Determining by Endoglin in Stage II Rectal Carcinoma: A Retrospective Analysis

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Zeljko Martinovic ◽  
Drazen Kovac ◽  
Mia Martinovic
Keyword(s):  
Rectal Cancer ◽  
Survival Rates ◽  
Prognostic Significance ◽  
Normal Mucosa ◽  
Tumor Location ◽  
Integrated System ◽  
Stage Ii ◽  
Rank Test ◽  
Adjacent Mucosa ◽  
Significant Difference

Background. The role of endoglin in the Dukes B rectal cancer is still unexplored. The aim of this study was to examine the expression of endoglin (CD105) in resected rectal cancer and to evaluate the relationship between microvessels density (MVD), clinicopathological factors, and survival rates.Methods. The study included 95 primary rectal adenocarcinomas, corresponding to 67 adjacent and 73 distant normal mucosa specimens from surgical resection samples. Tumor specimens were paraffin-embedded and immunohistochemical staining for the CD105 endothelial antigen was performed to count CD105-MVD. For exact measurement of the CD105-MVD used a computer-integrated system Alphelys Spot Browser 2 was used.Results. The intratumoral CD105-MVD was significantly higher compared with corresponding adjacent mucosa (P<0.0001) and distant mucosa specimens (P<0.0001). There was no significant difference in the CD105-MVD according to patients age, gender, tumor location, grade of differentiation, histological type, depth of tumor invasion, and tumor size. The overall survival rate was significantly higher in the low CD105-MVD group of patients than in the high CD105-MVD group of patients (log-rank test,P=0.0406).Conclusion. CD105-assessed MVD could help to identify patients with possibility of poor survival in the group of stage II RC.

The prognostic significance of IL-1-B and GST T1/M1 polymorphisms in non-metastatic gastric cancer

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 4084-4084
Author(s):  
M. S. Al-Moundhri ◽  
B. Al-Bahrani ◽  
M. Al-Nabhani ◽  
I. Burney ◽  
M. Al-Kindy ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Prognostic Value ◽  
Prognostic Significance ◽  
Stage Iv ◽  
Metastatic Gastric Cancer ◽  
Signet Ring ◽  
Distal Gastric Cancer ◽  
Gastric Cancer Patients ◽  
Pathological Stages

4084 Background: Gastric cancer is the most common malignancy in Oman. The proinflammatory cytokine IL-1-B polymorphisms have been associated with increased gastric cancer risk and shown to be of a prognostic value in advanced gastric cancer. Our aim is to study the prognostic significance of IL-1B- 31, -3954, IL-1RN- and GST T1/M1 polymorphisms in non-metastatic gastric cancer and correlate it with clinicopthological features. Methods: Genomic DNA was extracted from peripheral blood of 40 gastric cancer patients treated with adjuvant chemotherapy or chemoradiotherapy. The DNA samples were analyzed using TaqMan real-time polymerase chain reaction and 5’ nuclease assay. The deletion of GST T1/M1 genes was assessed by PCR. Results: The pathological stages were stage I = 1, stage II = 13, stage III = 22, stage IV = 3. The median follow up was 17 months. There was no prognostic significance for all the above polymorphisms in isolation. However, IL-1RN 2/2 IL-31 C/C genotypes (n = 13) were associated with worst outcome compared with IL-1RN L/L or 2/L and IL-31 T/T and T/C genotypes (n = 27). The median survival of IL-1RN 2/2 IL-31 C/C genotype was 16 months versus 63 months for IL-1RN L/L or 2/L and IL-31 T/T and T/C genotypes (p = 0.035). The IL-1RN 2/2 IL-31 C/C genotype correlated with signet ring pathology (p = 0.01) and non-distal gastric cancer location (p = 0.01). There was no significant association with T, N, or overall stage. Conclusion: These preliminary results suggest a prognostic value for IL-1-B polymorphisms in non-metastatic gastric cancer. No significant financial relationships to disclose.

Clinical utility of CEA, CA 19-9, and CA 72-4 in the follow-up of patients with resectable gastric cancer

2001 ◽  
Vol 181 (1) ◽  
pp. 16-19 ◽  
Author(s):  
Daniele Marrelli ◽  
Enrico Pinto ◽  
Alfonso De Stefano ◽  
Maurizio Farnetani ◽  
Lorenzo Garosi ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Clinical Utility ◽  
Resectable Gastric Cancer

Membranous Levels of C-Erbb-2 Oncoprotein in Gastric Cancer: Their Relationship with Clinicopathological Parameters and Their Prognostic Significance

2004 ◽  
Vol 19 (4) ◽  
pp. 268-274 ◽  
Author(s):  
F.J. Vizoso ◽  
M.D. Corte ◽  
A. Alvarez ◽  
I. García ◽  
J.M. del Casar ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Statistical Significance ◽  
Prognostic Significance ◽  
Clinicopathological Parameters ◽  
Clinical Value ◽  
Free Survival ◽  
Gastric Carcinomas ◽  
Novel Drug ◽  
Immunoenzymatic Assay

Background The protein encoded by the c-erbB-2 gene is a membrane receptor expressed in a variety of solid human cancers and directly related to poor prognosis. The objective of this work was to evaluate the clinical value of the quantification of membranous oncoprotein levels in gastric cancer. Materials and methods Membranous c-erbB-2 levels were examined by means of a sandwich immunoenzymatic assay in 82 patients with gastric cancer. The median follow-up period for these patients was 16 months. In addition, c-erbB-2 expression was analyzed by immunohistochemistry in 57 gastric carcinomas. Results Membranous c-erbB-2 levels ranged widely in the studied tumors (44–112,000 NHU/mg protein). Median c-erbB-2 content was significantly higher in intestinal-type tumors than in diffuse-type tumors (p=0.01). In addition, high levels of c-erbB-2 were significantly associated with shorter relapse-free survival and overall survival in patients with resectable gastric carcinomas (p=0.01 and p=0.04, respectively). However, the correlation between immunohistochemistry and ELISA determinations did not reach statistical significance. Conclusion Our results suggest a potential prognostic value of membranous c-erbB-2 quantification by immunoenzymatic assay in gastric cancer. However, its possible role in the selection of patients with a view to the possible introduction of Herceptin as a novel drug against gastric cancer is at present uncertain.

The opposite prognostic significance of nuclear and cytoplasmic p21 expression in resectable gastric cancer patients

2013 ◽  
Vol 49 (11) ◽  
pp. 1441-1452 ◽  
Author(s):  
Yefei Huang ◽  
Weimin Wang ◽  
Yansu Chen ◽  
Yulin Huang ◽  
Jianbing Zhang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
Prognostic Significance ◽  
Resectable Gastric Cancer ◽  
Gastric Cancer Patients

The prognostic significance of Cerb-B2 determination in gastric cancer.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. e14713-e14713
Author(s):  
Antonio CALDEIRA Fradique
Keyword(s):  
Gastric Cancer ◽  
Lymph Node ◽  
Lymph Node Metastases ◽  
Statistical Significance ◽  
Prognostic Significance ◽  
Subtotal Gastrectomy ◽  
D2 Lymphadenectomy ◽  
Tumor Aggressiveness ◽  
Resectable Gastric Cancer ◽  
Node Metastases

e14713 Background: The Cerb-B2/HER2 marker, is a glycoprotein encoded by the ERBB2 oncogene, belongs to the family of epidermal growth factor receptors (EGFR), which has based its application on tests of new therapeutic modalities (target therapy). Apart from this interest has been invoked its value as a marker of tumor aggressiveness, due to his role as a regulator of cell proliferation and migration and consequent invasiveness and metastization. However, the results as to the meaning prognosis of this molecular marker are controversial and with variations depending on the geographical zone. Results: From a total of 50 pts. with advanced resectable gastric cancer that underwent surgery with D2 lymphadenectomy, 38 pts. had a total gastrectomy, and 12 pts. had subtotal gastrectomy. A mean of 35.7 lymph nodes/pt were resected at surgery. In 68% of pts. positive lymph node metastases were present. Tumor HER2-neu overexpression was observed in 16 pts (32%), several other parameters of tumor aggressiveness were also present, without any statistical significance or relevance. Methods: To determine if the Cerb-B2 played a prognostic role in gastric cancer, a total of 50 pts. with advanced resectable gastric cancer, were submitted to gastrectomy with D2 lymphadenectomy. In all cases assessment for tumor HER2-neu overexpression was performed using immunohistochemistry (IHC). FISH was used as a confirmation method in tumors with 2+ expression by IHC. A correlation was made between the tumor HER2-neu expression and the following parameters: tumor location, size, histological type, depth of invasion, lymph node metastases and clinical staging. Results: From a total of 50 pts. with advanced resectable gastric cancer that underwent surgery with D2 lymphadenectomy, 38 pts. had a total gastrectomy, and 12 pts. had subtotal gastrectomy. A mean of 35.7 lymph nodes/pt were resected at surgery. In 68% of pts. positive lymph node metastases were present.Tumor HER2-neu overexpression was observed in 16 pts (32%), several other parameters of tumor aggressiveness were also present, without any statistical significance or relevance. Conclusions: The determination of Cerb-B2 / HER2 expression has not shown prognostic significance in this study.

Sign in / Sign up

Export Citation Format

Share Document