Ulipristal Acetate Antagonizes the Inhibitory Effect of Progesterone on Ciliary Beat Frequency and Upregulates Steroid Receptor Expression Levels in Human Fallopian Tubes

AbstractObjective:This study investigated the effect of Betadine on ciliated human respiratory epithelial cells.Methods:Epithelial cells from human sinonasal mucosa were cultured at the air–liquid interface. The cultures were tested with Hanks' balanced salt solution containing 10 mM HEPES (control), 100 µM ATP (positive control), 5 per cent Betadine or 10 per cent Betadine (clinical dose). Ciliary beat frequency was analysed using a high-speed camera on a computer imaging system.Results:Undiluted 10 per cent Betadine (n = 6) decreased the proportion of actively beating cilia over 1 minute (p < 0.01). Ciliary beat frequency decreased from 11.15 ± 4.64 Hz to no detectable activity. The result was similar with 5 per cent Betadine (n = 7), with no significant difference compared with the 10 per cent solution findings.Conclusion:Betadine, at either 5 and 10 per cent, was ciliotoxic. Caution should be applied to the use of topical Betadine solution on the respiratory mucosal surface.

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Estrogen replacement regimen and brain infusion of lipopolysaccharide differentially alter steroid receptor expression in the uterus and hypothalamus

Endocrine ◽

10.1007/s12020-008-9038-1 ◽

2007 ◽

Vol 32 (3) ◽

pp. 317-328 ◽

Cited By ~ 3

Author(s):

L. K. Marriott ◽

K. R. McGann-Gramling ◽

B. Hauss-Wegrzyniak ◽

L. C. Sheldahl ◽

R. A. Shapiro ◽

...

Keyword(s):

Steroid Receptor ◽

Receptor Expression ◽

Estrogen Replacement ◽

Steroid Receptor Expression

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Ovarian steroid receptor expression in endometriosis and in two potential parent epithelia: endometrium and peritoneal mesothelium

Human Reproduction ◽

10.1093/oxfordjournals.humrep.a137848 ◽

1992 ◽

Vol 7 (9) ◽

pp. 1318-1325 ◽

Cited By ~ 63

Author(s):

A. Prentice ◽

B. J. Randall ◽

A. Weddell ◽

A. McGill ◽

L. Henry ◽

...

Keyword(s):

Steroid Receptor ◽

Receptor Expression ◽

Ovarian Steroid ◽

Peritoneal Mesothelium ◽

Steroid Receptor Expression

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Effects of histamine on ciliary beat frequency of ciliated cells from guinea pigs nasal mucosa

European Archives of Oto-Rhino-Laryngology ◽

10.1007/s00405-014-3354-2 ◽

2014 ◽

Vol 272 (10) ◽

pp. 2839-2845 ◽

Cited By ~ 1

Author(s):

Fengwei An ◽

Lijun Xing ◽

Zhiqiang Zhang ◽

Lei Chen

Keyword(s):

Nasal Mucosa ◽

Guinea Pigs ◽

Beat Frequency ◽

Ciliary Beat Frequency ◽

Ciliated Cells ◽

Ciliary Beat

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Correlations among Mucociliary Transport, Ciliary Function, and Ciliary Structure

American Journal of Rhinology ◽

10.2500/105065898782102945 ◽

1998 ◽

Vol 12 (1) ◽

pp. 53-58 ◽

Cited By ~ 20

Author(s):

Mark Jorissen

Keyword(s):

Defense Mechanisms ◽

Ex Vivo ◽

Beat Frequency ◽

Ciliary Beat Frequency ◽

Preliminary Evidence ◽

Relevant Parameter ◽

Mucociliary Transport ◽

Ciliary Dyskinesia ◽

Ciliary Beat

Mucociliary transport is one of the most important defense mechanisms of the airway. Mucociliary transport time or rate, as measured using the saccharin test or the radioisotope technique, respectively, is clinically the most relevant parameter, although subject to large intra- and interindividual variability. There is no correlation between mucociliary transport in vivo and ciliary beat frequency ex vivo. Preliminary evidence demonstrates that mucociliary transport correlates with ciliary structure and orientation as investigated with transmission and scanning electron microscopy. A correlation is presented between ciliary beat frequency and secondary ciliary abnormalities. This correlation can best be described according to the logistic sigmoid model (r = 0.69). Based on these functional data, an ultrastructural distinction is proposed among normal (less than 5%), light (5 to 15%), moderate (15 to 25%), and severe (more than 25%) secondary ciliary dyskinesia.

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