scholarly journals Honokiol and Magnolol Production by in vitro Micropropagated Plants of Magnolia dealbata, an Endangered Endemic Mexican Species

2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Fabiola Domínguez ◽  
Marco Chávez ◽  
María Luisa Garduño-Ramírez ◽  
Víctor M. Chávez-Avila ◽  
Martín Mata ◽  
...  
Keyword(s):  
Growth Medium ◽  
Leaf Explants ◽  
Wild Plants ◽  
Root Induction ◽  
Ms Medium ◽  
Plant Materials ◽  
Detection Techniques ◽  
Magnolia Dealbata ◽  
Selection Of

An efficient protocol for the in vitro propagation of Magnolia dealbata Zucc., an important medicinal plant that is the source of the anxiolytic and anticancer compounds honokiol and magnolol, was established. This plant is wild-crafted, and conservationists have expressed concerns with regard to the sustainability of production. In the present work, two factors were found to be of importance for the regeneration of M. dealbata and the production of honokiol and magnolol. These factors were the type of explants and the combination and concentration of plant-growth regulators. Green, compact, nodular organogenic callus was obtained from leaf explants in a medium fortified with Murashige and Skoog salts and supplemented with 1.5 mg/L 2,4-dicholorophenoxyacetic acid and 1.5 mg/L kinetin. Shoot multiplication from callus cultures was achieved in the Murashige and Skoog (MS) medium with 1.5 mg/L thidiazuron (TDZ). Phenol secretion was controlled by the addition of 250 mg/L of activated charcoal. For rooting, shoots were transferred to MS medium supplemented with several auxins. After root induction, the plants were hardened in earthen pots containing sand, soil, and vermiculite. The contents of honokiol (HK) and magnolol (MG) were determined in different plant materials by high-performance liquid chromatography-diode-array detection techniques. This analysis revealed that the honokiol and magnolol content in aerial and underground parts of micropropagated M. dealbata were higher than that observed in wild plants (both 6 months old). Our results suggest that conservation of M. dealbata is possible by means of in vitro multiplication of leaf-derived callus. The usefulness of M. dealbata regeneration and production of HK and MG may be attributed to the proper selection of explant sourcing and identification of the correct growth medium to support adequate growth. This careful selection of explants and growth medium leads to a very useful source of plant material for pharmacological and phytomedicinal screening applications and, above all, would safeguard this plant species from the threat of extinction.

scholarly journals In vitro regeneration protocol of Rauvolfia serpentina L.

2018 ◽  
Vol 53 (2) ◽  
pp. 133-138 ◽  
Author(s):  
S Khan ◽  
TA Banu ◽  
S Akter ◽  
B Goswami ◽  
M Islam ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Regeneration System ◽  
Rauvolfia Serpentina ◽  
Number Of Shoots

An efficient in vitro regeneration system was developed for Rauvolfia serpentina L. through direct and indirect organogenesis from nodal and leaf explants. Among the different growth regulators, MS medium supplemented with 2.0 mg/l BAP, 0.5mg/l IAA and 0.02mg/l NAA found best for the multiple shoot formation from nodal segments. In this combination 98% explants produced multiple shoots and the average number of shoots per explants is 13∙4. The frequency of callus induction and multiple shoot induction from leaves was highest 88% in MS medium supplemented with 2.0 mg/l BAP, where mean number of shoots/explants was 12.5. The highest frequency of root induction (80%) and mean number of roots/plantlets (10) were obtained on half strength of MS medium containing 0.2 mg/l IBA. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Bangladesh J. Sci. Ind. Res.53(2), 133-138, 2018

scholarly journals Rapid In vitro Multiplication of Chirita longgangensis W.T. Wang: An Endemic and Endangered Gesneriaceae Species in China

HortScience ◽  
2007 ◽  
Vol 42 (3) ◽  
pp. 638-641 ◽  
Author(s):  
Zhenghui Tang ◽  
Honghui Lin ◽  
Lei Shi ◽  
Weilun Chen
Keyword(s):  
Acetic Acid ◽  
Somatic Embryos ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Basal Leaf ◽  
Second Stage ◽  
Regenerated Plantlets

Experiments were conducted to establish an efficient protocol for micropropagation of Chirita longgangensis W.T. Wang. Somatic embryos formed directly at the cut edges of C. longgangensis leaf explants on Murashige and Skoog (MS) medium supplemented with benzylaminopurine (BA) and α-naphthalene acetic acid (NAA). During the somatic embryo induction stage, leaf explants and basal leaf explants were used. Leaves were more appropriate explants than the basal leaf explants. The best medium was modified MS macronutrients and micronutrients supplemented with 0.5 mg·L−1 BA and 0.1 mg·L−1 NAA (the best mean number of somatic embryos per explants was 24.10 ± 1.63). The second stage was root induction and elongation. In vitro regenerated plantlets rooted best on MS medium containing 0.1 mg·L−1 indole-3-acetic acid (IAA) and 30 g·L−1 sucrose. Rooted plantlets, following acclimatization in a greenhouse, were successfully transferred to field conditions, and 95% of the plants survived. Application of this protocol has the ability for mass multiplication, in a limited time, of the endangered species C. longgangensis.

scholarly journals Preliminary Screening of Antioxidant and Antibacterial Activities and Establishment of an Efficient Callus Induction inCurculigo latifoliaDryand (Lemba)

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Reza Farzinebrahimi ◽  
Rosna Mat Taha ◽  
Kamaludin A. Rashid ◽  
Bakrudeen Ali Ahmed ◽  
Mahmoud Danaee ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Plant Regeneration ◽  
Leaf Explants ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Root Induction ◽  
Ms Medium ◽  
Preliminary Screening

Leaf, seed, and tuber explants ofC. latifoliawere inoculated on MS medium supplemented with various concentrations of BAP and IBA, alone or in combinations, to achievein vitroplant regeneration. Subsequently, antioxidant and antibacterial activities were determined fromin vitroandin vivoplant developed. No response was observed from seed culture on MS media with various concentrations of PGRs. The highest percentage of callus was observed on tuber explants (94%) and leaf explants (89%) when cultured on MS media supplemented with IBA in combination with BAP. A maximum of 88% shoots per tuber explant, with a mean number of shoots (8.8±1.0), were obtained on MS medium supplemented with combinations of BAP and IBA (2.5 mg L−1). The best root induction (92%) and mean number (7.6±0.5) from tuber explants were recorded on 2.5 mg L−1IBA alone supplemented to MS medium. The higher antioxidant content (80%) was observed fromin vivotuber. However, tuber part from the intact plant showed higher inhibition zone in antibacterial activity compared to otherin vitroandin vivotested parts.

scholarly journals IN VITRO DIRECT MULTIPLE SHOOT INDUCTION FROM LEAF EXPLANTS OF SOLANUM PUBESCENS WILLD

2016 ◽  
Vol 4 (12SE) ◽  
pp. 23-28
Author(s):  
Ayyadurai V ◽  
Ramar K
Keyword(s):  
Leaf Explants ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Root Induction ◽  
Ms Medium ◽  
Multiple Shoot Induction ◽  
Half Strength ◽  
Multiple Shoot Regeneration ◽  
Solanum Pubescens

Efficientin Vitro direct multiple shoot regeneration from Solanum pubescens was achieved from leaf explants on MS medium Sublimated with B5 vitamins and different concentrations and different combinations of PGRs like BAP, NAA and GA3. The maximum numbers of multiple shoots were achieved from leaf explants on 3.0 mg/l BAP + 1.0mg/l GA3. The regenerated shoots were transferred in to half strength MS medium fortified with IBA for root induction. Rooted plantlets were successfully acclimatized. This new and transfer into the field Conditions. Standardized and reproducible protocol useful the mass propagation of Solanum pubescens.

scholarly journals Induksi Kalus serta Regenerasi Tunas dan Akar Cabai melalui Kultur In Vitro

2016 ◽  
Vol 6 (2) ◽  
pp. 65
Author(s):  
Ifa Manzila ◽  
Sri H Hidayat ◽  
Ika Mariska ◽  
Sriani Sujiprihati
Keyword(s):  
Callus Induction ◽  
Embryogenic Callus ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Root Tip ◽  
Root Induction ◽  
Ms Medium ◽  
Root Tips ◽  
Culture Bottle

<p>Callus Induction and Regeneration of Shoot and Root of<br />Chill through In Vitro Culture. Ifa Manzila, Sri H.<br />Hidayat, Ika Mariska, and Sriani Sujiprihati. In vitro<br />culture is one way for a fast and effective plant propagation.<br />This method is also useful for preliminary selection of plant<br />resistance to disease, including the chili. In vitro propagation<br />method for chili has not been widely reported. A study was<br />conducted to obtain effective techniques for callus induction<br />and regeneration into shoots on three red chili cultivars (cv)<br />Gelora, Sudra, and Chili 109. The study consisted of four<br />activities, namely the induction of callus formation,<br />induction of embryogenic callus, callus regeneration into<br />adventitious shoots, and root induction from the adventitious<br />shoots. Murashige Skoog (MS) medium + 0.6% agar + 3%<br />sucrose were used as basal medium, 20 ml/bottle. Young<br />leaves, hypocotyls and root tips of 21-day-old chili seedlings<br />were used as sources of explants. Each experiment was<br />arranged in a completely randomized design with 10<br />replications, one culture bottle for each treatment. The<br />callus induction experiments using the explants of young<br />leaf explants, hypocotyl, and root tips were done separately.<br />Each treatment consisted of explants from the three chili<br />cultivars on MS medium containing three composition of<br />growth regulators (PGR) BAP + NAA, 10 explants/bottle. The<br />embryogenic callus induction was conducted by growing<br />the callus in bottles containing a medium that contains three<br />compositions PGR 2,4-D + thidiazuron 0.5 mg/l. Induction of<br />shoot formation was done by growing the embryogenic<br />callus on medium containing three composition of plant<br />growth regulator BAP + NAA. Induction of root formation<br />was performed by growing adventitious shoots on ½ MS and<br />1 MS medium + NAA 0.5 to 1.0 mg/l. The results showed that<br />young leaves are the best explant source for callus and<br />shoot formations in chili through tissue culture compared<br />with the hypocotyl and the tip. Gelora is the most responsive<br />chili cultivar to callus, shoots, and roots formation of in their<br />respective medium, compared to Sudra and Chile 109. MS<br />medium containing BAP 3-7 mg/ml and NAA 1 mg/ml can be<br />used to induce the growth of callus from young leaf<br />explants, hypocotyl and seedling root tip chili cv Gelora,<br />Sudra, and Chile 109, but its growth was very slow and did<br />not produce embryogenic callus. Embryogenic callus<br />formation can be induced by both non-embryogenic callus<br />Hak Cipta © 2010, BB-Biogen<br />growing the callus on MS medium containing 2,4-D 3 mg/l +<br />thidiazuron 0.5 mg/ l. Formation of callus that can regenerate<br />into shoots should use an MS medium containing 2,4-D 3<br />mg/l + thidiazuron 0.5 mg/ l followed by subculture on MS<br />medium + BAP 3 mg/l + thidiazuron 0.5 mg/l to induce<br />shoot elongation. Medium ½ MS and 1 MS containing NAA<br />0.5-1.0 mg/l can be used to induce root formations on shoot<br />culture of chili cv Gelora but not for cv Chili 109.</p>

MICROPROPAGATION OF CHICKPEA FROM SHOOT TIP AND NODE SEGMENT

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
J. D. BARSHILE
Keyword(s):  
Shoot Tip ◽  
Root Induction ◽  
Multiple Response ◽  
Ms Medium ◽  
Growth Responses ◽  
Rapid Multiplication ◽  
Micro Propagation ◽  
G 12 ◽  
Better Than

Present investigation was undertaken to standardize technique for in vitro micro-propagation of chickpea( Cicer arietinum ) cultivar Vishwas (Phule G 12). Micropropagation method for chickpea was established and this method enabled much more efficient propagation of plants. The present work was aimed at evolving a protocol for rapid multiplication of chickpea using micropropagation technique. Explants from shoot tip and node segment were cultured on MS medium supplemented with different concentrations of BAP and Kinetin (1.0 to 2.5 mg/l) and their growth responses like shooting were elucidated. The maximum multiple response was observed with 2 mg/l concentration of BAP from both types of explant. The highest number of shoots (12.5 ± 0.3) was achieved on MS medium with 2 mg/l BAP using node segments. The medium supplemented with 2 mg/l of BAP was found better than all other concentrations. Individual shoots were transferred to IBA and IAA (1.0-1.5 mg/l) for root induction. MS medium supplemented with 2 mg/l of IBA proved better for rooting. Rooted plantlets were successfully hardened in greenhouse and established in the pot.

scholarly journals Shoot Multiplication and Callus Induction of Labisia pumila var. alata as Influenced by Different Plant Growth Regulators Treatments and Its Polyphenolic Activities Compared with the Wild Plant

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3229
Author(s):  
Mat Yunus Najhah ◽  
Hawa Z. E. Jaafar ◽  
Jaafar Juju Nakasha ◽  
Mansor Hakiman
Keyword(s):  
Callus Induction ◽  
Antioxidant Activities ◽  
Shoot Multiplication ◽  
Wild Plants ◽  
Wild Plant ◽  
Total Phenolic ◽  
Nodal Segment ◽  
Ms Medium ◽  
In Vitro Plantlets

This study aims to investigate whether the in vitro-cultured L. pumila var. alata has higher antioxidant activity than its wild plant. An 8-week-old L. pumila var. alata nodal segment and leaf explants were cultured onto Murashige and Skoog (MS) medium supplemented with various cytokinins (zeatin, kinetin, and 6-benzylaminopurine (BAP)) for shoot multiplication and auxins (2,4-dichlorophenoxyacetic acid (2,4-D) and picloram) for callus induction, respectively. The results showed that 2 mg/L zeatin produced the optimal results for shoot and leaf development, and 0.5 mg/L 2,4-D produced the highest callus induction results (60%). After this, 0.5 mg/L 2,4-D was combined with 0.25 mg/L cytokinins and supplemented to the MS medium. The optimal results for callus induction (100%) with yellowish to greenish and compact texture were obtained using 0.5 mg/L 2,4-D combined with 0.25 mg/L zeatin. Leaves obtained from in vitro plantlets and wild plants as well as callus were extracted and analyzed for their antioxidant activities (DPPH and FRAP methods) and polyphenolic properties (total flavonoid and total phenolic content). When compared with leaf extracts of in vitro plantlets and wild plants of L. pumila var. alata, the callus extract displayed significantly higher antioxidant activities and total phenolic and flavonoid content. Hence, callus culture potentially can be adapted for antioxidant and polyphenolic production to satisfy pharmaceutical and nutraceutical needs while conserving wild L. pumila var. alata.

scholarly journals Production of bergenin, an active chemical constituent in the callus of Bergenia ciliata (Haw.) Sternb.

2012 ◽  
Vol 8 ◽  
pp. 40-44 ◽  
Author(s):  
Umesh Krishna Shrestha ◽  
Bijaya Pant
Keyword(s):  
Leaf Explants ◽  
Chemical Constituent ◽  
Wild Plants ◽  
Plant Science ◽  
Naphthalene Acetic Acid ◽  
Active Chemical ◽  
In The Wild ◽  
The Media ◽  
Benzyl Amino Purine

In vitro culture of Bergenia ciliata (Haw.) Sternb. was carried out for the examination of bergenin content. Leaf explants were cultured in MS (Murashige and Skoog) basal media supplemented with or without phytohormones. The hormonal series maintained were in the range of 0-2 mg l-1 for BAP (6-benzyl amino purine) and 0-1.5 mg l-1 for NAA (α-naphthalene acetic acid). Bergenin content of in vitro grown tissues of B. ciliata was compared with that of wild plants collected from three different localities of Nepal. The best growth of callus and plantlets occurred in the media containing BAP 1.0 mg l-1 + NAA 1.0 mg l-1 and BAP 1.5 mg l-1 + NAA 1.0 mg l-1. Production of bergenin was high in the media supplemented with 1.0 mg l-1 BAP + 1.5 mg l-1 NAA (3.40 μg g-1) and 2.0 mg l-1 BAP + 1.5 mg l-1 NAA (3.05 μg g-1) under experimental condition. The bergenin content in the wild plants collected from Langtang, Jumla and Godawari was found to be 4.28 μg g-1, 4.53 μg g-1 and 3.64 μg g-1 respectively. This study shows that the in vitro cultured callus of B. ciliata is capable of synthesizing bergenin in quantity comparable to that of the wild plant.doi: http://dx.doi.org/10.3126/botor.v8i0.5557 Botanica Orientalis – Journal of Plant Science (2011) 8: 40-44

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals IN VITRO REGENERATION AND MASS PROPAGATION OF AZIMA TETRACANTHA [LAM.] FROM THE LEAF EXPLANTS THROUGH CALLUS CULTURE

2017 ◽  
Vol 4 (2) ◽  
pp. 52-56
Author(s):  
Mallika Devi T
Keyword(s):  
Callus Induction ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Shoot Formation ◽  
Ms Medium ◽  
Apical Leaf ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Azima Tetracantha

In the present study the protocol for callus induction and regeneration in Azima tetracantha has been developed in culture medium. The young apical leaf explants were used for callus induction on MS medium containing BAP and NAA at 1.0 and 0.4mgl-1 respectively showed maximum callus induction (73%). The amount of callus responded for shoot formation (74%) was obtained in the MS medium containing BAP (1.5 mgl-1) and NAA (0.3mgl-1).The elongated shoots were rooted on half strength medium supplemented with IBA (1.5 mgl-1) and Kn (0.4 mgl-1) for shoots rooted. Regenerated plantlets were successfully acclimatized and hardened off inside the culture and then transferred to green house with better survival rate.

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