Chemical Composition and Antimicrobial Activity of Essential Oil of Heracleum rigens

The chemical composition and antimicrobial activity of the essential oil from the aerial parts of Pyrethrum pulchrum Ledeb. were investigated. Dried plant material was hydro-distillated yielding 0.1% of essential oil. The oil was analyzed by GC-MS techniques. Fifty-five compounds were identified representing 99.7% of the total oil composition. Camphor was the predominant compound (33.9%) followed by linalool (21.1%) and α-pinene (9.0%). The antimicrobial activity of the oil was determined using the disk diffusion method against Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus and Enterococcus faecalis), Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), Mycobacterium vaccae and fungi (Candida albicans, Sporidiobolus salmonicolor and Penicillum notatum). The essential oil of P. pulchrum displays an intermediate activity against selected bacteria.

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Chemical Composition and Biological Activity of the Essential Oil from Jamaican Cleome serrata

Natural Product Communications ◽

10.1177/1934578x1200700934 ◽

2012 ◽

Vol 7 (9) ◽

pp. 1934578X1200700 ◽

Cited By ~ 1

Author(s):

Megil J. McNeil ◽

Roy B. R. Porter ◽

Lawrence A. D. Williams

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Oil Composition ◽

Filter Paper Disc ◽

Aerial Parts ◽

Main Components ◽

Total Oil

The chemical composition of the essential oil obtained from the aerial parts of Cleome serrata by hydrodistillation was analyzed by employing GC-FID, GC-MS and RI. Fourteen compounds comprising 90.4% of the total oil composition were characterized. The main components identified were ( Z)-phytol (53.0%) and di(2-ethylhexyl)-phthalate (DEHP) (14.7%). The oil was evaluated for its in vitro antimicrobial activities against nine pathogenic microorganisms using the filter paper disc diffusion method. Moderate antimicrobial activity was observed against five of the pathogens assayed. In addition, the essential oil was tested against the sweet potato weevil, Cylas formicarius elegantulus. Strong knockdown insecticidal activity was observed.

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Chemical Composition and Antimicrobial Activity of Essential oil from Scales of Moroccan Juniperus phoenicea

Universities' Journal of Phytochemistry and Ayurvedic Heights ◽

10.51129/ujpah-june2021-30-1(1) ◽

2021 ◽

Vol 1 (30) ◽

pp. 5-11

Author(s):

M. Achir M. Dakir

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Antifungal Agent ◽

Antibacterial Agent ◽

Oil Composition ◽

High Biological Activity ◽

Juniperus Phoenicea ◽

Total Oil ◽

Antibacterial And Antifungal

Abstract-The essential oil of Juniperus phoenicea was obtained by hydrodistillation method using a Clevenger-type apparatus with a yield of 1.9 % and was analyzed by gas chromatography coupled to a mass spectrometer (GC-MS). Twelve volatile compounds were identified representing 99,85% of the total oil composition, while the α-pinene (78,31%), β-Myrcene (11,92%) and limonene (3,96%) were the major compounds. This essential oil was evaluated as an antibacterial and antifungal agent. The result showed that the oil presents a high biological activity as an antibacterial agent against the three tested strains Escherichia coli, Staphylococcus aureus and Pseudomonas aeroginos. It's also active as an antifungal agent against the Candida albicans with a zone inhibition of 28 mm. Keywords: Medicinal plant, Juniperus, phoenicea, Essential oil, Chemical composition, Antimicrobial activity.

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Chemical Composition and Antibacterial Activity of Melaleuca bracteata Essential Oil from India: A Natural Source of Methyl Eugenol

Natural Product Communications ◽

10.1177/1934578x1701200633 ◽

2017 ◽

Vol 12 (6) ◽

pp. 1934578X1701200 ◽

Cited By ~ 1

Author(s):

Prakash Goswami ◽

Sajendra K. Verma ◽

Amit Chauhan ◽

KT Venkatesha ◽

Ram S. Verma ◽

...

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Methyl Eugenol ◽

Klebsiella Pneumonia ◽

Moderate Activity ◽

Bacterial Strains ◽

Indian Origin ◽

Total Oil ◽

Autumn And Winter

The aim of this study was to investigate the chemical composition and antibacterial activity of the essential oil of Melaleuca bracteata F. Muell from India. The hydrodistilled essential oils of M. bracteata in spring, summer, rainy, autumn and winter seasons were analyzed using GC-FID and GC-MS. Altogether, 25 constituents, comprising 97.3–99.7% of the total oil were identified with methyl eugenol (87.2–89.5%), ( E)-methyl cinnamate (2.8–5.4%), methyl chavicol (0.2–0.5%), and elemicin (0.2–0.4%) as major constituents. The antibacterial efficacy of the essential oil was evaluated against nine pathogenic bacterial strains. Zone of inhibition and MIC of the essential oil ranged from 5–10 mm and 500–1000 μg/mL against the tested pathogens, respectively. Results showed that essential oil of M. bracteata from Indian origin possessed good activity against Salmonella typhimurium, Staphylococcus epidermidis, and moderate activity against Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumonia, and Streptococcus mutans. Results of the present seasonal variation study clearly indicated that M. bracteata grown in foothills of Uttarakhand, India could be classified as seasonally stable ‘methyl eugenol’ chemotype (>85.0%), hence may be considered as a novel natural resource for methyl eugenol rich essential oil for flavor, fragrance and pharmaceutical applications.

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INVESTIGATION INTO THE ANTIBACTERIAL ACTIVITY AND CHEMICAL COMPOSITION OF ‘CEDRUS ATLANTICA’ ESSENTIAL OIL

INDIAN DRUGS ◽

10.53879/id.55.06.11292 ◽

2018 ◽

Vol 55 (06) ◽

pp. 51-55

Author(s):

N Belloula ◽

◽

S. Dridi ◽

A Khattaf

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Chemical Constituents ◽

Volatile Oil ◽

Diffusion Method ◽

Klebsiella Pneumonia ◽

Oil Composition ◽

Cedrus Atlantica

In the present study, Cedrus atlantica leaves volatile oil were screened for their antibacterial activity and chemical composition. The essential oil chemical constituents obtained by hydro-distillation and analyzed by gas chromatography coupled with mass spectrometry (GC-MS). The antibacterial activity was tested in vitro on Gram-negative bacteria: Escherichia coli, Pseudomonas and Klebsiella pneumonia and Gram-positive: Staphylococcus aureus, Bacillus, and Enterococcus faecalis, using disc-diffusion method. Twenty five compounds were identified in leaves oil representing 66.0.9 % of the total oil composition. Cedrus atlantica essential oil yield was 1.64% and major compounds were α-pinene (8,65%), himachalene (8.41%), β-himachalene (8,14%), σ-himachalene (6,26%), cis-α-atlantone (5,11%), himachalol (4,25%) and α-himachalene (3,25%), germacrene D (3.15%), β-caryophyllene (2.14%), cadinene (2.00%), β-pinene (1.95%), humulene (1.20%) and β-copaene (1.16%). In both strain, Cedrus atlantica oil exhibited very considerable antibacterial activity.

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Quality testing of industrially produced essential oil of white pine (Pinus sylvestris L.) from the Republic of Srpska

JOURNAL OF ENGINEERING & PROCESSING MANAGEMENT ◽

10.7251/jepm2002036b ◽

2020 ◽

Vol 12 (2) ◽

Author(s):

Vesna Gojković Cvjetković ◽

Željka Marjanović-Balabań ◽

Marijana Kapović-Solomuń ◽

Ljiljaná́ Stanojevic ◽

Jelená́ Stanojevic ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Diffusion Method ◽

Bacterial Strains ◽

White Pine ◽

Disk Diffusion Method ◽

Sesquiterpene Hydrocarbons ◽

Zones Of Inhibition ◽

The Republic

The quality of the essential oil obtained from white pine wood greenery from the territory of the Republic of Srpska was analyzed by examining the chemical composi- tion and antimicrobial activity of samples produced by the hydro-distillation process in industrial production conditions. The chemical composition of the essential oil was analyzed by GC/MS and GC/FID analysis. The antimicrobial activity of the ob- tained white pine oil was investigated for its possible use as an antimicrobial agent. The obtained results prove the presence of 37 components in the analyzed sam- ple, which represents 98.5% of the total components present. The most common are monoterpene hydrocarbons, followed by sesquiterpene hydrocarbons, much less represented are monoterpenes containing oxygen and aromatic compounds, while aliphatic esters are found only in traces. Antimicrobial activity was examined using the disk diffusion method. The results showed large zones of inhibition, both for the tested oil sample and for all eight analyzed bacterial strains (from 12.00 ± 0.00 to 17.33 ± 1.15mm), as well as for the fungal strain. The chemical composition and antimicrobial action of essential white pine oiled from plant material originating from the territory of the Republic of Srpska indicates its significant phytomedical potential.

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CHEMICAL COMPOSITION, ANTIMICROBIAL, AND ANTIOXIDANT ACTIVITIES OF TWO MOROCCAN THYMUS ESSENTIAL OILS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i1.30377 ◽

2019 ◽

Vol 12 (1) ◽

pp. 447 ◽

Cited By ~ 3

Author(s):

Imane Aouam ◽

Yassine El Atki ◽

Amal Taroq ◽

Fatima El Kamari ◽

Abdelfattah Abdellaoui

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Diffusion Method ◽

Bornyl Acetate ◽

Total Phenolic ◽

Klebsiella Pneumonia ◽

Bacterial Strains ◽

Antioxidant Power

Objective: The aim of the present study is to investigate chemical constituents of Thymus riatarum and Thymus blecherianus essential oils (EOs) as well as to evaluate, for the first time, their antioxidant effect and antibacterial activity against six bacterial strains responsible for nosocomial infections.Methods: The chemical composition of EOs was analyzed using gas chromatography (GC) and GC-mass spectrometry, the antibacterial capacity of the two thymus species was evaluated against six bacteria species: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Acinetobacter baumannii, and Citrobacter sp. using disk diffusion method and microdilution assay. Finally, the antioxidant activity was measured by four different test systems of assay, namely free radical scavenging activities, ferric reducing/antioxidant power assays, total phenolic, and flavonoid contents.Results: A total of 15 compounds representing 99.6% of T. riatarum oil were identified with thymol (28.8%), borneol (20.0%), and α-phellandrene (13.0%) as the main constituents. Eleven components of T. blecherianus were identified representing 98.2% of the total oil composition; the most abundant constituents were as follows: Carvacrol (45.9%), bornyl acetate (20.1%), and borneol (15.7%). Strong antibacterial activity of the two EOs was identified against all bacterial strains tested. Concerning the antioxidant results, T. riatarum EO exhibited higher antioxidant activity than T. blecherianus in the three assays with an IC50 value equal to 5.75±0.06 mg/ml, which was probably due to its high content of polyphenols (28.95±0.13 mg GAE/g DW). Total flavonoid content was found equal for the two EOs.Conclusion: EO of T. riatarum and T. blecherianus from Morocco can be exploited as a natural antibacterial and antioxidant new potential sources.

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Chemical Composition and Antimicrobial Activity of the Essential Oil of Anaphalis nubigena Var. Monocephala

Natural Product Communications ◽

10.1177/1934578x0900400725 ◽

2009 ◽

Vol 4 (7) ◽

pp. 1934578X0900400 ◽

Cited By ~ 4

Author(s):

Rajesh K. Joshi ◽

Chitra Pande ◽

Mohammad H. K. Mujawar ◽

Sanjiva D. Kholkute

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Klebsiella Pneumoniae ◽

Essential Oil Composition ◽

Oil Composition ◽

Aerial Parts ◽

Sesquiterpene Hydrocarbons ◽

Total Oil

The essential oil composition of the aerial parts of Anaphalis nubigena DC. var. monocephala (DC.) C. B. Clarke collected from Pindari glacier at a height of 3300 m, was analyzed by using GC and GC/MS. Sixty components were identified, accounting for 95.9% of the total oil. The main constituents were α-guaiene (12.3%), γ-muurolene (10.4%), γ-cadinene (8.3%), α-muurolol (7.4%), α-gurjunene (6.0%) and α-bulnesene (5.8%). The oil was found to be rich in sesquiterpene hydrocarbons (60.1%). The oil was active against Escherichia coli (NCIM 2065) and Klebsiella pneumoniae (NCIM 2957), with MIC values of 125 μg/mL and 500 μg/mL, respectively.

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CHEMICAL COMPOSITION, ANTIMICROBIAL, AND ANTIOXIDANT ACTIVITIES OF TWO MOROCCAN THYMUS ESSENTIAL OILS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v12i1.30377 ◽

2019 ◽

Vol 12 (1) ◽

pp. 447 ◽

Cited By ~ 1

Author(s):

Imane Aouam ◽

Yassine El Atki ◽

Amal Taroq ◽

Fatima El Kamari ◽

Abdelfattah Abdellaoui

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Diffusion Method ◽

Bornyl Acetate ◽

Total Phenolic ◽

Klebsiella Pneumonia ◽

Bacterial Strains ◽

Antioxidant Power

Objective: The aim of the present study is to investigate chemical constituents of Thymus riatarum and Thymus blecherianus essential oils (EOs) as well as to evaluate, for the first time, their antioxidant effect and antibacterial activity against six bacterial strains responsible for nosocomial infections.Methods: The chemical composition of EOs was analyzed using gas chromatography (GC) and GC-mass spectrometry, the antibacterial capacity of the two thymus species was evaluated against six bacteria species: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Acinetobacter baumannii, and Citrobacter sp. using disk diffusion method and microdilution assay. Finally, the antioxidant activity was measured by four different test systems of assay, namely free radical scavenging activities, ferric reducing/antioxidant power assays, total phenolic, and flavonoid contents.Results: A total of 15 compounds representing 99.6% of T. riatarum oil were identified with thymol (28.8%), borneol (20.0%), and α-phellandrene (13.0%) as the main constituents. Eleven components of T. blecherianus were identified representing 98.2% of the total oil composition; the most abundant constituents were as follows: Carvacrol (45.9%), bornyl acetate (20.1%), and borneol (15.7%). Strong antibacterial activity of the two EOs was identified against all bacterial strains tested. Concerning the antioxidant results, T. riatarum EO exhibited higher antioxidant activity than T. blecherianus in the three assays with an IC50 value equal to 5.75±0.06 mg/ml, which was probably due to its high content of polyphenols (28.95±0.13 mg GAE/g DW). Total flavonoid content was found equal for the two EOs.Conclusion: EO of T. riatarum and T. blecherianus from Morocco can be exploited as a natural antibacterial and antioxidant new potential sources.

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New Insight into the Chemical Composition, Antimicrobial and Synergistic Effects of the Moroccan Endemic Thymus atlanticus (Ball) Roussine Essential Oil in Combination with Conventional Antibiotics

Molecules ◽

10.3390/molecules26195850 ◽

2021 ◽

Vol 26 (19) ◽

pp. 5850

Author(s):

Ahmed Nafis ◽

Marcello Iriti ◽

Lahcen Ouchari ◽

Fatima El Otmani ◽

Najat Marraiki ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Synergistic Effect ◽

Synergistic Effects ◽

Micrococcus Luteus ◽

Diffusion Method ◽

Klebsiella Pneumonia ◽

Conventional Antibiotics ◽

And Control

This study reported the volatile profile, the antimicrobial activity and the synergistic potential of essential oil (EO) from the Moroccan endemic Thymus atlanticus (Ball) Roussine, in combination with the antibiotics ciprofloxacin and fluconazole for the first time, to the best of our knowledge. The EO chemical composition was determined by gas chromatography coupled to mass spectrometry (GC-MS) analysis and the antimicrobial activity assessed by the disc diffusion method against three Gram positive (Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus) and three Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli and one clinical isolate, Klebsiella pneumonia). The antifungal activity was evaluated in four pathogenic yeasts (Candida albicans, C. glabrata, C. krusei and C. parapsilosis). The minimum inhibition concentration (MIC) and the synergistic effect with ciprofloxacin and fluconazole were determined by the two-fold dilution technique and checkerboard test, respectively. Twenty-one constituents were identified by GC-MS in the EO, including carvacrol (21.62%) and borneol (21.13%) as the major components. The EO exhibited a significant antimicrobial activity with inhibition zones ranging from 0.7 mm to 22 mm for P. aeruginosa and B. subtilis, respectively, and MIC values varying from 0.56 mg/mL to 4.47 mg/mL. The fractional inhibitory concentration index (FICI) values ranged from 0.25 to 0.50 for bacteria and from 0.25 to 0.28 for yeasts. The maximum synergistic effect was observed for K. pneumonia with a 256-fold gain of antibiotic MIC. Our results have suggested that EO from T. atlanticus may be used alone or in association with antibiotics as a new potential alternative to prevent and control the emergence of resistant microbial strains both in the medical field and in the food industry.

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