scholarly journals Tirucallane Glycoside from the Leaves of Antidesma bunius and Inhibitory NO Production in BV2 Cells and RAW264.7 Macrophages

2016 ◽  
Vol 11 (7) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Do Thi Trang ◽  
Le Thi Huyen ◽  
Nguyen Xuan Nhiem ◽  
Tran Hong Quang ◽  
Dan Thi Thuy Hang ◽  
...  
Keyword(s):  
Triterpene Glycoside ◽  
Methanol Extract ◽  
Inhibitory Effect ◽  
Published Data ◽  
No Production ◽  
Spectroscopic Methods ◽  
Raw264.7 Macrophages ◽  
Bv2 Cells ◽  
Megastigmane Glycosides ◽  
Strong Inhibitory Effect

One new tirucallane- type triterpene glycoside, antidesoside (1), along with two biflavones, podocarpusflavone A (2) and amentoflavone (3) and two megastigmane glycosides, byzantionoside B (4), and (6 S,9 R)-roseoside (5) were isolated from the methanol extract of Antidesma bunius leaves. Their structures were determined by spectroscopic methods and in comparison with the published data. Compounds 1 - 3 were found to show strong inhibitory effect of NO production in BV2 cells and RAW264.7 macrophages LPS-stimulated, with IC50 values ranging from 8.5 to 26.9 μM.

scholarly journals Chemical Constituents of Euonymus glabra

2015 ◽  
Vol 10 (12) ◽  
pp. 1934578X1501001
Author(s):  
Jie Ren ◽  
Yang-Guo Xie ◽  
Xing Wang ◽  
Shi-Kai Yan ◽  
Hui-Zi Jin ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Phenolic Compound ◽  
Chemical Constituents ◽  
No Production ◽  
Spectroscopic Methods ◽  
Inhibitory Effects ◽  
Raw264.7 Macrophages ◽  
Circular Dichroism Analysis ◽  
Inhibitory Activities ◽  
Circular Dichroïsm

One new phenolic compound (1) and one new flavan (2), together with eight known compounds (3–10) were isolated from the stems and twigs of Euonymus glabra Roxb. Their structures were elucidated mainly on the basis of 1D and 2D spectroscopic methods and circular dichroism analysis. In addition, compounds 1–10 were tested for their inhibitory effects against LPS-induced NO production in RAW264.7 macrophages. Compounds 1–5 and 7 exhibited moderate inhibitory activities with IC50 values ranged from 5.1 to 11.9 μM.

scholarly journals Anti-Inflammatory Investigations of Extracts of Zanthoxylum rhetsa

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Chureeporn Imphat ◽  
Pakakrong Thongdeeying ◽  
Arunporn Itharat ◽  
Sumalee Panthong ◽  
Sunita Makchuchit ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
No Production ◽  
Water Distillation ◽  
Raw264.7 Macrophages ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Zanthoxylum rhetsa has been consumed in the diet in northern Thailand and also used as a medicament in ancient scripture for arthropathies. Thus, this study aimed to evaluate the activity of various extracts from differential parts of Z. rhetsa via inhibition of inflammatory mediators (NO, TNF-α, and PGE2) in RAW264.7 macrophages. The chemical composition in active extracts was also analyzed by GC/MS. The parts of this plant studied were whole fruits (F), pericarp (P), and seed (O). The methods of extraction included maceration in hexane, 95% ethanol and 50% ethanol, boiling in water, and water distillation. The results demonstrated that the hexane and 95% ethanolic extract from pericarp (PH and P95) and seed essential oil (SO) were the most active extracts. PH and P95 gave the highest inhibition of NO production with IC50 as 11.99 ± 1.66 μg/ml and 15.33 ± 1.05 μg/ml, respectively, and they also showed the highest anti-inflammatory effect on TNF-α with IC50 as 36.08 ± 0.55 μg/ml and 34.90 ± 2.58 μg/ml, respectively. PH and P95 also showed the highest inhibitory effect on PGE2 but less than SO with IC50 as 13.72 ± 0.81 μg/ml, 12.26 ± 0.71 μg/ml, and 8.61 ± 2.23 μg/ml, respectively. 2,3-Pinanediol was the major anti-inflammatory compound analyzed in PH (11.28%) and P95 (19.82%) while terpinen-4-ol constituted a major anti-inflammatory compound in SO at 35.13%. These findings are the first supportive data for ethnomedical use for analgesic and anti-inflammatory activity in acute (SO) and chronic (PH and P95) inflammation.

scholarly journals FLAVONOIDS AND TRITERPENOIDS FROM CALLISTEMON CITRINUS AND THEIR INHIBITORY EFFECT ON NO PRODUCTION IN LPS-STIMULATED RAW264.7 MACROPHAGES

2016 ◽  
Vol 54 (2) ◽  
pp. 214 ◽  
Author(s):  
Nguyen Manh Cuong ◽  
Pham Ngoc Khanh ◽  
Ho Viet Duc ◽  
Tran Thu Huong ◽  
Youn-Chui Kim ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
2D Nmr ◽  
Inhibitory Effect ◽  
Raw264.7 Cells ◽  
No Production ◽  
Inhibition Activity ◽  
Chemical Structures ◽  
Raw264.7 Macrophages ◽  
Phytochemical Investigation ◽  
Methoxy Flavone

Phytochemical investigation of the leaves and stems of Callistemon citrinus (Curtis) Skeels led to the isolation of 12 flavonoid and triterpenoid compounds,  including one new flavonoid, callistine A (1) and six known flavonoids 6,7- dimethyl-5,7-dihydroxy-4’-methoxy flavone (2), astragalin (3), quercetin (4), catechin (5), eucalyptin (6), and 8-demethyleucalyptin (7), along with 5 triterpenoids, 3-β-acetylmorolic acid (8), 3β-hydroxy-urs-11-en-13(28)-olide (9), betulinic acid (10), diospyrolide (11) and ursolic acid (12). Their chemical structures were determined from the spectroscopic evidences counting 1D- and 2D-NMR and HR-MS data. All the isolated compounds were examined for their anti-inflammatory activity against LPS-activated NO production in macrophage RAW264.7 cells. Among them, quercetin (4) and 3β-hydroxy-urs-11-en-13(28)-olide (9) showed potential inhibition activity in nitric oxide (NO)  production in RAW264.7 cells exposed to LPS.

Anti-inflammatory components isolated from Atractylodes macrocephala in LPS-induced RAW264.7 macrophages and BV2 microglia

2021 ◽  
Author(s):  
Hong-Quang Jin ◽  
Kwan-Woo Kim ◽  
Jing Li ◽  
Dae Young Lee ◽  
Dahye Yoon ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Published Data ◽  
No Production ◽  
Caffeoylquinic Acid ◽  
Raw264.7 Macrophages ◽  
Phytochemical Investigation ◽  
Tnf Α ◽  
Bv2 Microglia ◽  
Atractylodes Macrocephala ◽  
Oxide Synthase

Abstract The phytochemical investigation on the methanol extract of the rhizomes of Atractylodes macrocephala resulted in the discovery of one new compound 9α-hydroxyatractylenolide (1) and 21 known compounds including atractylone (2), 3β-acetoxyatractylon (3), atractylenolide I (4), atractylenolide II (5), 8-epiasterolid (6), atractylenolide III (7), atractylenolide VII (8), 8-epiatractylenolide III (9), eudesm-4(15)-ene-7α,11-diol (10), linoleic acid (11), myristic acid (12), 3-O-caffeoyl-1-methyquinic acid (13), (2E,8E,10E)-tetradecatriene-4,6-diyne-1,14-diol (14), 14-aceroxy-12-senecioyloxytetradeca-2E,8Z,10E-trien-4,6-diyn-1-ol (15), isoscopoletin (16), caffeic acid (17), protocatechic acid (18), 3-O-caffeoylquinic acid (19), 4-O-caffeoylquinic acid (20), 1,5-di-O-caffeoylquinic acid (21), and nicotinic acid (22). Their structures were identified using nuclear magnetic resonance (NMR) and mass spectroscopy, and by comparison with previously published data. Compounds 4, 5, 6, 8, and 10–22 significantly inhibited lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 macrophages, and compounds 4, 5, 6, 16, and 17 showed those responses in BV2 microglial cells. Especially, compound 6 showed the second-best effect, and inhibited the LPS-induced production of prostaglandin E2 (PGE2), the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, and the production of cytokines including interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in both cells. These inhibitory effects were mediated by the inactivation of nuclear factor kappa B (NF-κB) signaling pathway.

scholarly journals Characterization of Anti-Inflammatory and Antioxidant Constituents from Scutellaria baicalensis Using LC-MS Coupled with a Bioassay Method

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3617
Author(s):  
Yoo Kyong Han ◽  
Hyunwoo Kim ◽  
Hyeji Shin ◽  
Jiyeon Song ◽  
Mi Kyeong Lee ◽  
...  
Keyword(s):  
Screening Method ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Scutellaria Baicalensis ◽  
No Production ◽  
Dpph Assay ◽  
Bioactive Constituents ◽  
Total Extract ◽  
Molecular Networking ◽  
Bv2 Cells

An effective and previously demonstrated screening method for active constituents in natural products using LC-MS coupled with a bioassay was reported in our earlier studies. With this, the current investigation attempted to identify bioactive constituents of Scutellaria baicalensis through LC-MS coupled with a bioassay. Peaks at broadly 17–20 and 24–25 min on the MS chromatogram displayed an inhibitory effect on NO production in lipopolysaccharide-induced BV2 microglia cells. Similarly, peaks at roughly 17–19 and 22 min showed antioxidant activity with an 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)/2,2-diphenyl-1- picrylhydrazyl (DPPH) assay. For confirmation of LC-MS coupled with a bioassay, nine compounds (1–9) were isolated from an MeOH extract of S. baicalensis. As we predicted, compounds 1, 8, and 9 significantly reduced lipopolysaccharide (LPS)-induced NO production in BV2 cells. Likewise, compounds 5, 6, and 8 exhibited free radical-scavenging activities with the ABTS/DPPH assay. In addition, the structural similarity of the main components was confirmed by analyzing the total extract and EtOAc fractions through molecular networking. Overall, the results suggest that the method comprised of LC-MS coupled with a bioassay can effectively predict active compounds without an isolation process, and the results of molecular networking predicted that other components around the active compound node may also be active.

scholarly journals A Cytotoxic Flavanone from The Pod Peels of Theprosia vogelii Hook.f.

2020 ◽  
Vol 6 (2) ◽  
pp. 140-145
Author(s):  
Valentina Adimurti Kusumaningtyas ◽  
Nia Sri Hardianti ◽  
Melina Melina ◽  
Lia Dewi Juliawaty ◽  
Yana Maolana Syah
Keyword(s):  
Brine Shrimp ◽  
Methanol Extract ◽  
Published Data ◽  
Spectroscopic Methods ◽  
Cytotoxic Property ◽  
Brine Shrimp Lethality ◽  
The Family ◽  
Tephrosia Vogelii ◽  
Chemical Evidence

Tephrosia vogelii Hook.f. is a species of the family Fabaceae (Leguminoceae). These plants are termed ″Polong-polongan″ in Indonesia, and are known to contain active flavonoid groups. Previous studies have shown the isolation of one known flavanone: isolonchocarpin from methanol extract, and the structure obtained was established based on chemical evidence as well as spectroscopic methods, including NMR, and also by a comparison with published data. This research is aimed at evaluating the cytotoxic property of methanol extract against larvae of Arthemia salina Leach, using the Brine Shrimp Lethality Test (BSLT) method. The results show potent cytotoxicity at LC50 of 41.40 ppm.

scholarly journals Anti-Inflammatory Effect and Mechanism of the Green Fruit Extract ofSolanum integrifoliumPoir.

2014 ◽  
Vol 2014 ◽  
pp. 1-11
Author(s):  
Lisu Wang ◽  
Shu-Yuan Chiou ◽  
Yi-Ting Shen ◽  
Fu-Tsun Yen ◽  
Hsiou-Yu Ding ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Inflammatory Activity ◽  
Zinc Protoporphyrin ◽  
No Production ◽  
Fruit Extract ◽  
Methanol Fraction ◽  
Green Fruit ◽  
Raw264.7 Macrophages ◽  
Fractionation Procedure ◽  
Anti Inflammatory

The green fruit ofSolanum integrifoliumPoir. has been used traditionally as an anti-inflammatory and analgesic remedy in Taiwanese aboriginal medicine. The goal of this study is to evaluate the anti-inflammatory activity and mechanism of the green fruit extract ofS. integrifolium. A bioactivity-guided fractionation procedure was developed to identify the active partition fraction. The methanol fraction (ME), with the highest phenolic content, exhibited the strongest inhibitory effect against LPS-mediated nitric oxide (NO) release and cytotoxicity in RAW264.7 macrophages. ME also significantly downregulated the expression of LPS-induced proinflammatory genes, such as iNOS, COX-2, IL-1β, IL-6, CCL2/MCP-1, and CCL3/MIP1α. Moreover, ME significantly upregulated HO-1 expression and stimulated the activation of extracellular-signal-regulated kinase 1/2 (ERK1/2). Pretreatment of cells with the HO-1 inhibitor zinc protoporphyrin and MEK/ERK inhibitor U0126 attenuated ME’s inhibitory activity against LPS-induced NO production. Taken together, this is the first study to demonstrate the anti-inflammatory activity of green fruit extract ofS. integrifoliumand its activity may be mediated by the upregulation of HO-1 expression and activation of ERK1/2 pathway.

scholarly journals New Acetylated Saponins from the Leaves of Trevesia palmata

2018 ◽  
Vol 13 (4) ◽  
pp. 1934578X1801300
Author(s):  
Le Thi Thanh Thao ◽  
Do Quyen ◽  
Duong Binh Vu ◽  
Bui Huu Tai ◽  
Phan Van Kiem
Keyword(s):  
Alkaline Hydrolysis ◽  
Nmr Spectra ◽  
Inhibitory Effect ◽  
No Production ◽  
Esi Ms ◽  
Chemical Structures ◽  
Bv2 Cells ◽  
Weak Activity

Two new acetylated saponins, acetyltrevesiasaponins A (1) and B (2), were isolated from the leaves of Trevesia palmata. Their chemical structures were determined by analysis of HR-ESI-MS and NMR spectra. Compound 2 was also confirmed by alkaline hydrolysis and identification its prosapogenin 2a. Compounds 1, 2, and 2a were evaluated inhibitory effect on NO production in LPS stimulated BV2 cells. Compound 2 showed weak activity (IC50 40.7 μM).

Inhibitory effect of isolated constituents from sclerotia of Poria cocos on LPS-induced NO production

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
SR Lee ◽  
S Lee ◽  
HJ Eom ◽  
HR Kang ◽  
JS Yu ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
No Production ◽  
Poria Cocos
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