scholarly journals New Acetylated Saponins from the Leaves of Trevesia palmata

2018 ◽  
Vol 13 (4) ◽  
pp. 1934578X1801300
Author(s):  
Le Thi Thanh Thao ◽  
Do Quyen ◽  
Duong Binh Vu ◽  
Bui Huu Tai ◽  
Phan Van Kiem
Keyword(s):  
Alkaline Hydrolysis ◽  
Nmr Spectra ◽  
Inhibitory Effect ◽  
No Production ◽  
Esi Ms ◽  
Chemical Structures ◽  
Bv2 Cells ◽  
Weak Activity

Two new acetylated saponins, acetyltrevesiasaponins A (1) and B (2), were isolated from the leaves of Trevesia palmata. Their chemical structures were determined by analysis of HR-ESI-MS and NMR spectra. Compound 2 was also confirmed by alkaline hydrolysis and identification its prosapogenin 2a. Compounds 1, 2, and 2a were evaluated inhibitory effect on NO production in LPS stimulated BV2 cells. Compound 2 showed weak activity (IC50 40.7 μM).

scholarly journals New Truxinic and Truxillic Acid Sucrose Diesters From the Leaves of Trigonostemon honbaensis

2021 ◽  
Vol 16 (2) ◽  
pp. 1934578X2199914
Author(s):  
Ninh Khac Ban ◽  
Bui Huu Tai ◽  
Vu Kim Thu ◽  
Phan Van Kiem
Keyword(s):  
Nmr Spectra ◽  
Raw264.7 Cells ◽  
No Production ◽  
Inhibitory Effects ◽  
Esi Ms ◽  
Chemical Structures ◽  
Extensive Analysis ◽  
Truxillic Acid

A new δ-truxinic acid sucrose diester and a new ε-truxillic acid sucrose diester (named trigohonbanosides E and F) were isolated from the leaves of Trigonostemon honbaensis. Their chemical structures were determined by extensive analysis of their HR-ESI-MS and NMR spectra. At a concentration of 20 µM, trigohonbanosides E and F exhibited weak inhibitory effects on NO production in LPS-activated RAW264.7 cells with inhibitory percentages of 22.7% ± 1.1% and 18.5% ± 1.4%, respectively.

scholarly journals New Acetylated Terpenoids from Sponge Rhabdastrella providentiae Inhibit NO Production in LPS Stimulated BV2 Cells

2018 ◽  
Vol 13 (6) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Duong Thi Dung ◽  
Pham Hai Yen ◽  
Nguyen Xuan Nhiem ◽  
Tran Hong Quang ◽  
Bui Huu Tai ◽  
...  
Keyword(s):  
2D Nmr ◽  
No Production ◽  
Esi Ms ◽  
Chemical Structures ◽  
Bv2 Cells

Three new acetylated terpenoids, rhabdaprovidines A-C (1-3), were isolated from the Vietnamese sponge Rhabdastrella providentiae. Their chemical structures were established by HR-ESI-MS, 1D and 2D-NMR experiments. These compounds share 6,6,5-tricyclic nucleus of isomalabaricane-type triterpene, the specific secondary constituents from Rhabdastrella species. Compounds 1-3 inhibited NO production in LPS stimulated BV2 cells with IC50 values of 20.4 ± 1.5, 17.5 ± 0.9, and 46.8 ± 2.3 μM, respectively.

scholarly journals New Alkaloids and Anti-inflammatory Constituents from the Leaves of Antidesma ghaesembilla

2017 ◽  
Vol 12 (1) ◽  
pp. 1934578X1701200
Author(s):  
Phan Van Kiem ◽  
Le Canh Viet Cuong ◽  
Do Thi Trang ◽  
Nguyen Xuan Nhiem ◽  
Hoang Le Tuan Anh ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Inhibitory Activity ◽  
No Production ◽  
Raw 264.7 ◽  
Chemical Methods ◽  
Raw 264.7 Macrophages ◽  
Chemical Structures ◽  
Bv2 Cells ◽  
Inhibitory Activities ◽  
Physical And Chemical

Using various chromatographic methods, two new alkaloids, antidesoic acids A (1) and B (2) along with fourteen known compounds (3-16) were isolated from the leaves of Antidesma ghaesembilla Gaertn. Their chemical structures were elucidated by physical and chemical methods. All the isolated compounds were evaluated for their inhibitory activity on LPS-stimulated nitric oxide (NO) production in BV2 cells and RAW 264.7 macrophages. Bisflavone 8 significantly inhibited LPS-stimulated NO production in BV2 cells and RAW 264.7 macrophages with IC50 values of 5.4 and 8.0 μM, respectively. Compounds 1–3, 7, 10, 12, 14, and 16 showed moderate inhibitory activities with IC50 values ranging from 11.7 to 77.4 μM.

scholarly journals A New Megastigmane Glucoside and Other Constituents from Desmodium gangeticum

2020 ◽  
Vol 2020 ◽  
pp. 1-4
Author(s):  
Hau Viet Dang ◽  
Giang Hoang Do ◽  
Phuong Thi Ngo ◽  
Tien Dat Nguyen ◽  
Ha Minh Le
Keyword(s):  
Nmr Spectra ◽  
Aerial Part ◽  
2D Nmr ◽  
Inhibitory Effect ◽  
Methyl Benzoate ◽  
Raw264.7 Cells ◽  
No Production ◽  
2D Nmr Spectra ◽  
Ic50 Values ◽  
Megastigmane Glucoside

A new megastigmane glycoside, gangeticoside (1), and three known compounds leonuriside A (2), methyl benzoate 2-O-β-D-glucopyranoside (3), and tortoside A (4) were isolated from the aerial part of Desmodium gangeticum. Their structures were determined by 1D and 2D NMR spectra. The isolated compounds were evaluated for their inhibitory effect on NO production in LPS-stimulated RAW264.7 cells. Among them, compounds 1, 2, and 3 exhibited strong effect with the IC50 values of 22.3, 15.6, 7.3 μM, respectively.

scholarly journals FLAVONOIDS AND TRITERPENOIDS FROM CALLISTEMON CITRINUS AND THEIR INHIBITORY EFFECT ON NO PRODUCTION IN LPS-STIMULATED RAW264.7 MACROPHAGES

2016 ◽  
Vol 54 (2) ◽  
pp. 214 ◽  
Author(s):  
Nguyen Manh Cuong ◽  
Pham Ngoc Khanh ◽  
Ho Viet Duc ◽  
Tran Thu Huong ◽  
Youn-Chui Kim ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
2D Nmr ◽  
Inhibitory Effect ◽  
Raw264.7 Cells ◽  
No Production ◽  
Inhibition Activity ◽  
Chemical Structures ◽  
Raw264.7 Macrophages ◽  
Phytochemical Investigation ◽  
Methoxy Flavone

Phytochemical investigation of the leaves and stems of Callistemon citrinus (Curtis) Skeels led to the isolation of 12 flavonoid and triterpenoid compounds,  including one new flavonoid, callistine A (1) and six known flavonoids 6,7- dimethyl-5,7-dihydroxy-4’-methoxy flavone (2), astragalin (3), quercetin (4), catechin (5), eucalyptin (6), and 8-demethyleucalyptin (7), along with 5 triterpenoids, 3-β-acetylmorolic acid (8), 3β-hydroxy-urs-11-en-13(28)-olide (9), betulinic acid (10), diospyrolide (11) and ursolic acid (12). Their chemical structures were determined from the spectroscopic evidences counting 1D- and 2D-NMR and HR-MS data. All the isolated compounds were examined for their anti-inflammatory activity against LPS-activated NO production in macrophage RAW264.7 cells. Among them, quercetin (4) and 3β-hydroxy-urs-11-en-13(28)-olide (9) showed potential inhibition activity in nitric oxide (NO)  production in RAW264.7 cells exposed to LPS.

scholarly journals Tirucallane Glycoside from the Leaves of Antidesma bunius and Inhibitory NO Production in BV2 Cells and RAW264.7 Macrophages

2016 ◽  
Vol 11 (7) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Do Thi Trang ◽  
Le Thi Huyen ◽  
Nguyen Xuan Nhiem ◽  
Tran Hong Quang ◽  
Dan Thi Thuy Hang ◽  
...  
Keyword(s):  
Triterpene Glycoside ◽  
Methanol Extract ◽  
Inhibitory Effect ◽  
Published Data ◽  
No Production ◽  
Spectroscopic Methods ◽  
Raw264.7 Macrophages ◽  
Bv2 Cells ◽  
Megastigmane Glycosides ◽  
Strong Inhibitory Effect

One new tirucallane- type triterpene glycoside, antidesoside (1), along with two biflavones, podocarpusflavone A (2) and amentoflavone (3) and two megastigmane glycosides, byzantionoside B (4), and (6 S,9 R)-roseoside (5) were isolated from the methanol extract of Antidesma bunius leaves. Their structures were determined by spectroscopic methods and in comparison with the published data. Compounds 1 - 3 were found to show strong inhibitory effect of NO production in BV2 cells and RAW264.7 macrophages LPS-stimulated, with IC50 values ranging from 8.5 to 26.9 μM.

scholarly journals Steroidal Glucosides from the Rhizomes of Tacca Chantrieri and Their Inhibitory Activities of NO Production in BV2 Cells

2016 ◽  
Vol 11 (1) ◽  
pp. 1934578X1601100
Author(s):  
Pham Hai Yen ◽  
Vu Thi Quynh Chi ◽  
Dong-Cheol Kim ◽  
Wonmin Ko ◽  
Hyuncheol Oh ◽  
...  
Keyword(s):  
2D Nmr ◽  
No Production ◽  
Esi Ms ◽  
Bv2 Cells ◽  
Inhibitory Activities ◽  
Reported Data

Two new steroidal glucosides, chantriolides D and E (1 and 2), along with four known compounds, chantriolide A (3), chantriolide B (4), chantriolide C (5), and (25 S)-spirost-5-en-3-ol 3- O-α-L-rhamnopyranosyl-(1→2)- O-[α-L-rhamnopyranosyl-(1→3)]-β-D-glucopyranoside (6) were isolated from the rhizomes of Tacca chantrieri. Their structures were determined by 1D and 2D NMR spectroscopic and HR-ESI-MS data, as well as by comparison with reported data. Compounds 1 and 2 were found to show strong inhibitory NO effect in BV2 cells, with IC50 values of 12.45 and 59.03 μM, respectively.

scholarly journals Bioactive compounds from an endophytic fungi Nigrospora aurantiaca

2021 ◽  
Vol 62 (1) ◽  
Author(s):  
Safwan Safwan ◽  
George Hsiao ◽  
Tzong-Huei Lee ◽  
Ching-Kuo Lee
Keyword(s):  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Spectroscopic Data ◽  
Biological Activities ◽  
Inhibitory Effect ◽  
Fungal Strain ◽  
Biologically Active ◽  
No Production ◽  
Chemical Structures ◽  
Melaleuca Leucadendra

Abstract Background Many groups of fungi live as an endophyte in plants. Both published and undiscovered bioactive compounds can be found in endophytic fungi. Various biological activities of bioactive compounds from endophytic fungi had been reported, including anti-inflammatory and anticancerous effects. The chemical investigation of biologically active compounds from endophytic fungi Melaleuca leucadendra Linn. have not yet been stated. Results One new compound, namely nigaurdiol (1), along with five known compounds, xyloketal K (2), bostrycin (3), deoxybostrycin (4), xylanthraquinone (5), and ergosterol (6), were isolated from the Melaleuca leucadendra Linn. associated fungal strain Nigrospora aurantiaca#TMU062. Their chemical structures were elucidated by spectroscopic data and compared with literature. All isolated compounds were evaluated for inhibitory effect of NO production in LPS-activated microglial BV-2 cells. Conclusions Compound 6 exhibited considerable inhibitory effect on NO production with IC50 values of 7.2 ± 1.4 µM and the survival rate of the cells was 90.8 ± 6.7% at the concentration of 10 µM.

A Neuroinflammation Inhibitor, Hypoxylon xanthone A, from Soil Fungus Hypoxylon sp.

2020 ◽  
Vol 17 (2) ◽  
pp. 116-120
Author(s):  
Ying Xiao ◽  
Bing Xu ◽  
You Kang ◽  
Yuedi Li ◽  
Yong Cui ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Nmr Spectra ◽  
Soil Fungus ◽  
2D Nmr ◽  
Inhibitory Effect ◽  
No Production ◽  
Dependent Manner ◽  
Solid Culture ◽  
2D Nmr Spectra ◽  
Dose Dependent

: Hypoxylon sp. was used to ferment at 25°C for 45 days. The solid culture of Hypoxylon sp. was extracted with 75% EtOH under ultrasonic for twice. And the dried combined extracts were then suspended in H2O and partitioned with ethyl acetate. EtOAc extracts were subjected to a silica gel column and eluted with petroleum ether - acetone to a.ord seven fractions. Sephadex LH-20 and RPHPLC were used subsequently to yield a novel xanthone metabolite (Hypoxylon xanthone A). Its structure was elucidated based on HR-ESI-MS, 1D-, 2D-NMR spectra, and the comparison of the experimental and calculated ECD spectra. The anti-neuroinflammatory assay of Hypoxylon xanthone A, as manifested by the inhibitory effect on LPS-induced NO production in BV-2 microglial cells, indicated almost the same inhibitory effect as minocycline in a dose-dependent manner within the concentration of 1-50 μM, suggesting that Hypoxylon xanthone A could be a new potential neuroinflammation inhibitor.

scholarly journals Characterization of Anti-Inflammatory and Antioxidant Constituents from Scutellaria baicalensis Using LC-MS Coupled with a Bioassay Method

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3617
Author(s):  
Yoo Kyong Han ◽  
Hyunwoo Kim ◽  
Hyeji Shin ◽  
Jiyeon Song ◽  
Mi Kyeong Lee ◽  
...  
Keyword(s):  
Screening Method ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Scutellaria Baicalensis ◽  
No Production ◽  
Dpph Assay ◽  
Bioactive Constituents ◽  
Total Extract ◽  
Molecular Networking ◽  
Bv2 Cells

An effective and previously demonstrated screening method for active constituents in natural products using LC-MS coupled with a bioassay was reported in our earlier studies. With this, the current investigation attempted to identify bioactive constituents of Scutellaria baicalensis through LC-MS coupled with a bioassay. Peaks at broadly 17–20 and 24–25 min on the MS chromatogram displayed an inhibitory effect on NO production in lipopolysaccharide-induced BV2 microglia cells. Similarly, peaks at roughly 17–19 and 22 min showed antioxidant activity with an 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)/2,2-diphenyl-1- picrylhydrazyl (DPPH) assay. For confirmation of LC-MS coupled with a bioassay, nine compounds (1–9) were isolated from an MeOH extract of S. baicalensis. As we predicted, compounds 1, 8, and 9 significantly reduced lipopolysaccharide (LPS)-induced NO production in BV2 cells. Likewise, compounds 5, 6, and 8 exhibited free radical-scavenging activities with the ABTS/DPPH assay. In addition, the structural similarity of the main components was confirmed by analyzing the total extract and EtOAc fractions through molecular networking. Overall, the results suggest that the method comprised of LC-MS coupled with a bioassay can effectively predict active compounds without an isolation process, and the results of molecular networking predicted that other components around the active compound node may also be active.

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