scholarly journals Natural Cembrane Diterpenoids From the Soft Coral Sinularia querciformis

2021 ◽  
Vol 16 (11) ◽  
pp. 1934578X2110592
Author(s):  
Yi-Ying Wu ◽  
Chia-Ching Hsieh ◽  
Chia-Ying Li ◽  
Wen-Huei Chang ◽  
Jih-Jung Chen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Inducible Nitric Oxide Synthase ◽  
Soft Coral ◽  
Nmr Analysis ◽  
Chemical Examination ◽  
H Nmr ◽  
Mouse Macrophages ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide ◽  
C Nmr

Chemical examination of Sinularia querciformis afforded one new cembranoid, querciformolide F (1), along with four known cembranoids, sinulariolone (2), granosolide A (3), querciformolide A (4), and sinulariolide (5). The structures of these compounds were determined by extensive spectroscopic (IR, ESIMS, 1H NMR, and 13C NMR) analysis and by comparison with those previously reported in the literature. Compounds 2 to 4 were found to exhibit significant anti-inflammatory activity in lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages through attenuating the expression of the inducible nitric oxide synthase (iNOS) proteins.

Cloning and characterization of inducible nitric oxide synthase from mouse macrophages

Science ◽  
1992 ◽  
Vol 256 (5054) ◽  
pp. 225-228 ◽  
Author(s):  
Q. Xie ◽  
H. Cho ◽  
J Calaycay ◽  
R. Mumford ◽  
K. Swiderek ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Mouse Macrophages ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin and related flavonoids in mouse macrophages

1999 ◽  
Vol 20 (10) ◽  
pp. 1945-1952 ◽  
Author(s):  
Yu-Chih Liang ◽  
Ying-Tang Huang ◽  
Shu-Huei Tsai ◽  
Shoei-Yn Lin-Shiau ◽  
Chieh-Fu Chen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Mouse Macrophages ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Inhibition of inducible nitric oxide synthesis by the herbal preparation Padma 28 in macrophage cell line

2000 ◽  
Vol 78 (11) ◽  
pp. 861-866 ◽  
Author(s):  
Thomas Moeslinger ◽  
Roswitha Friedl ◽  
Ivo Volf ◽  
Monika Brunner ◽  
Elisabeth Koller ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Inos Mrna ◽  
Mrna Levels ◽  
Nitric Oxide Synthesis ◽  
Mouse Macrophages ◽  
Oxide Synthase ◽  
Padma 28 ◽  
Inducible Nitric Oxide

Padma 28 is a mixture of herbs used in traditional Tibetan medicine with anti-inflammatory activities. We investigated the effects of Padma 28 on nitric oxide (NO) production by the inducible nitric oxide synthase (iNOS) in lipopolysaccharide stimulated mouse macrophages (RAW 264.7). Padma 28 (0-900 µg/mL) induced a concentration dependent inhibition of inducible nitric oxide synthesis. iNOS protein expression showed a concentration dependent reduction as revealed by immunoblotting when cells were incubated with increasing amounts of Padma 28. Padma 28 decreased iNOS mRNA levels as shown by RT-PCR. Aqueous extracts from costi amari radix (costus root, the dried root of Saussurea lappa) and the outer cover of myrobalani fructus (the dried fruit of Terminalia chebula), constituents of the complex herb preparation Padma 28, were found to inhibit inducible nitric oxide synthesis by decreasing iNOS protein and iNOS mRNA levels. The inhibition of inducible nitric oxide synthesis might contribute to the anti-inflammatory activities of Padma 28.Key words: inducible nitric oxide synthase, mouse macrophages, myrobalans, radix costae.

scholarly journals Effects of dichloromethane Sarcophyton spp. extract on the lipopolysaccharide-induced expression of nuclear factor-kappa B and inducible nitric oxide synthase in mice

2019 ◽  
Vol 12 (12) ◽  
pp. 1897-1902 ◽  
Author(s):  
Putut Har Riyadi ◽  
Didik Wahyudi ◽  
Wendy Alexander Tanod
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Nuclear Factor Kappa B ◽  
Soft Coral ◽  
Relative Number ◽  
Inos Expression ◽  
Nuclear Factor ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Background and Aim: The soft coral genus Sarcophyton is a source of cembraneterpen. Sarcophyton is reported to have anti-inflammatory properties, with the ability to reduce the expression of inducible nitric oxide synthase (iNOS) and inhibit nuclear factor-kappa B (NF-κB) activation. This study aimed to investigate the efficacy of dichloromethane (DCM) extracts of soft coral Sarcophyton spp. to inhibit the expression of NF-κB and iNOS induced by lipopolysaccharide (LPS). Materials and Methods: Crude extracts of Sarcophyton spp. were macerated with DCM (1:3 v/v) for 24 h. Thirty-six Balb/c mice were divided into six treatment groups, namely, normal control (without LPS induction), negative control (LPS induction 4 mg/mL), comparative control (LPS+Dexamethasone 6 mg/kg), and 3 concentration groups extract (LPS+50, 125, and 250 mg/kg). The expression of NF-κB and iNOS was measured in each treatment group. Results: Flow cytometry analysis showed that the relative number of NF-κB+ cells increased (18.38±1.24%) in LPS-induced mice compared with normal mice (13.24±1.15%). The Sarcophyton spp. DCM extracts decreased the relative number of NF-κB+ cells (125 mg/kg: 13.96±0.84%). Immunohistochemical analysis with ImmunoMembrane showed that LPS induction in mice increased iNOS expression when compared to normal mice. The Sarcophyton spp. DCM extracts reduced iNOS expression (especially at 125 mg/kg). Conclusion: DCM extracts of Sarcophyton spp. inhibited the activation of NF-κB, resulting in suppressed iNOS expression, which directly inhibits NO production.

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