scholarly journals Flavonoids of the Heartwood of Cotinus coggygria Scop. Showing Protective Effect on Human Lymphocyte DNA

2021 ◽  
Vol 16 (12) ◽  
pp. 1934578X2110672
Author(s):  
Slobodan Milosavljevic ◽  
Iris Djordjevic ◽  
Boris Mandic ◽  
Vele Tesevic ◽  
Miroslava Stankovic ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Protective Effect ◽  
Methylene Chloride ◽  
Methanol Extract ◽  
Human Lymphocytes ◽  
Micronucleus Assay ◽  
Protective Activity ◽  
Cotinus Coggygria ◽  
Better Than

In continuation of our study on Cotinus coggygria from Serbia, 10 known flavonoids (1-10) were isolated from the methylene chloride/methanol extract of the heartwood. They were tested for in vitro protective effect against chromosome aberrations in peripheral human lymphocytes, using the cytokinesis-block micronucleus assay. All tested compounds (in minimal doses of 1 μg/mL) exerted a beneficial effect by decreasing DNA damage of human lymphocytes in the range of 24.2% to 54.5%, better than the radio protectant control, amifostine. Functional groups, such as 3′,4′-dihydroxyphenyl (catechol), 5-OH, 3-OH, and 4-keto in flavonoids (3-keto in aurones), which play a key role in antioxidant activity, are proposed to be responsible for the DNA protective activity of the tested compounds.

scholarly journals DNA protective activity of triterpenoids isolated from medicinal mushroom Fomitopsis betulina

2021 ◽  
pp. 39-39
Author(s):  
Ivana Sofrenic ◽  
Boban Andjelkovic ◽  
Ljubodrag Vujisic ◽  
Miroslav Novakovic ◽  
Aleksandar Knezevic ◽  
...  
Keyword(s):  
Protective Effect ◽  
Human Lymphocytes ◽  
Acid Group ◽  
Positive Control ◽  
Dependent Manner ◽  
Protective Activity ◽  
Substitution Pattern ◽  
Concentration Dependent Manner ◽  
Fomitopsis Betulina

Eleven 31-methylenlanostane triterpenoids, i.e. seven 21- and four 26-oic acids, as well as a lupane triterpenoid betulin, isolated from the fruiting bodies of the mushroom Fomitopsis betulina were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. Most of the tested compounds exerted a beneficial effect by reducing DNA damage of human lymphocytes more effectively than amifostine, a radioprotective agent, used as a positive control. All the tested compounds decreased MN frequency in concentration dependent manner, with the concentration of 2.0 ?g mL-1 being the most effective - with increase of the concentration the activity slightly decreases. The structure-activity relationship (SAR) studies indicated that the lanostanes containing a conjugated 7,9 (11)-diene system exhibit lower activity than D8-analogues. It was also demonstrated that the DNA protective activities within the D8-lanostane-26-oic acid group are affected by 3-substitution pattern. In the D8 series the oxygenation at C-12 or 16 as well as 21- or 26-oic acid functionality proved beneficial for in vitro protective effect on chromosomal aberrations. Betulin exhibited the lowest protective activity, but still comparable to that of amifostine.

scholarly journals Micronucleus assay in human lymphocytes after exposure to alloxydim sodium herbicide in vitro

2014 ◽  
Vol 67 (6) ◽  
pp. 1059-1066 ◽  
Author(s):  
Dilek Akyıl ◽  
Arzu Özkara ◽  
S. Feyza Erdoğmuş ◽  
Yasin Eren ◽  
Muhsin Konuk ◽  
...  
Keyword(s):  
Human Lymphocytes ◽  
Micronucleus Assay

Prophylactic and Therapeutic Treatments' Effect of Moringa Oleifera Methanol Extract on Cryptosporidium Infection in Immunosuppressed Mice

2019 ◽  
Vol 17 (2) ◽  
pp. 130-137 ◽  
Author(s):  
Nagwa M. El-Sayed ◽  
Ghada M. Fathy
Keyword(s):  
Antioxidant Activity ◽  
Moringa Oleifera ◽  
Prophylactic Treatment ◽  
Methanol Extract ◽  
Villous Atrophy ◽  
Inflammatory Cells ◽  
Cryptosporidium Oocysts ◽  
Ifn Γ

<P>Background: As natural herbs and medicinal plants extracts are widely used as alternative treatments for different parasitic diseases, some have been tested on Cryptosporidium either in vitro or in vivo. This study assessed the prophylactic and therapeutic treatments' effect of Moringa oleifera (M. oleifera) leaves methanol extract on immunosuppressed-Cryptosporidium infected mice. Methods: The evaluation was carried out by Cryptosporidium oocysts count in fecal samples, histopathological changes in the intestinal tissues, determination of IFN-γ level in mice sera and measuring the antioxidant activity in the intestinal tissues. Results: Prophylactic treatment by M. oleifera extract lowered Cryptosporidium oocysts shedding from immunosuppressed-infected mice although there was no complete elimination of the parasite. However, therapeutic treatment induced a significant reduction in Cryptosporidium oocysts counts by 91.8% higher than that of the drug control (nitazoxanide) group (77.2%). Histopathologically, the intestinal tissues from immunosuppressed-Cryptosporidium infected mice showed loss of brush border with severe villous atrophy and extensive necrosis. M. oleifera prophylactic treatment induced a moderate improvement of the pathological changes. However, the villi in M. oleifera therapeutic treated mice retained their normal appearance with minimal inflammatory cells. It was observed that M. oleifera extract induced a significant upregulation of IFN-γ in both prophylactic and therapeutic treated groups compared to that of the infected untreated group. In addition, M. oleifera leaves extract exhibited a significant antioxidant activity by reducing the levels of Nitric Oxide (NO) and Malondialdehyde (MDA) and increasing Superoxide Dismutase (SOD) level in the intestinal tissues compared to those of the infected and drug controls. Conclusion: M. oleifera leaves extract has potent prophylactic and therapeutic activities against infection with Cryptosporidium.</P>

scholarly journals In vitro antioxidant, anti-inflammatory and anti-cancer activities of methanolic extract of Asparagus horridus grows in North Cyprus Kuzey Kıbrıs da yetişen Asparagus horridus metanolik ekstraktının in-vitro antioksidan, anti-enflamatuar ve anti-kanser aktivitesi

2020 ◽  
Vol 45 (4) ◽  
pp. 365-372
Author(s):  
Ergul Mutlu Altundag ◽  
Duygu Gençalp ◽  
Cahit Özbilenler ◽  
Kübra Toprak ◽  
Namık Kerküklü
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Protein Denaturation ◽  
Soxhlet Extraction ◽  
Edible Plant ◽  
North Cyprus ◽  
Anti Cancer ◽  
Anti Inflammatory ◽  
Inflammatory Effect

AbstractBackgroundAsparagus horridus is an edible plant known as “Ayrelli” in North Cyprus. The scientific literature has not yet submitted a report about the antioxidant, anti-inflammatory and anti-cancer activities of A. horridus plant from North Cyprus until now. The purpose of the research was to determine the antioxidant, anti-inflammatory and anti-cancer activities of A. horridus.Materials and methodsSoxhlet extraction of A. horridus was performed using methanol. Antioxidant activity was determined by DPPH, TFC, FRAP and TPC assays. Protein-denaturation assay was performed to determine the anti-inflammatory effect. The anti-cancer effects of the extract on HepG2 and B-CPAP cell lines were determined with MTT assay.ResultsAntioxidant activity for A. horridus extract was determined by DPPH (50%), TFC (266.26 μg QUE/mg extract), FRAP (1.27 μg FeSO4/mg extract) and TPC (167.613 μg GAE/mg extract) assays at 25 mg/mL. Inhibition of protein-denaturation activity was found as 29.42% at 25 mg/mL. After 24 h of the extract treatment, cell proliferation of HepG2 and B-CPAP cancer cells were inhibited at IC50 values 63.24 μg/mL and 101.24 μg/mL, respectively.ConclusionThese results have shown that the methanol extract of A. horridus grows in North Cyprus has antioxidant, anti-inflammatory and anti-cancer activities.

Methanol extracts of Teucrium arduini L. and Teucrium flavum L. induce protective effect against mitomycin C in human lymphocytes in vitro

2020 ◽  
pp. 1-7
Author(s):  
Aleksandra Marković ◽  
Jovana Tubić Vukajlović ◽  
Darko Grujičić ◽  
Marina Radović Jakovljević ◽  
Milan Stanković ◽  
...  
Keyword(s):  
Protective Effect ◽  
Mitomycin C ◽  
Human Lymphocytes ◽  
Methanol Extracts

scholarly journals IN VITRO ANTIOXIDANT AND IN VIVO ANTI-INFLAMMATORY ACTIVITY OF THE AERIAL PART OF BLUMEA ERIANTHA DC

2018 ◽  
Vol 10 (7) ◽  
pp. 75 ◽  
Author(s):  
Urmila U. Tambewagh ◽  
Supada Rambhau Rojatkar
Keyword(s):  
Antioxidant Activity ◽  
Aerial Part ◽  
Methanol Extract ◽  
Reducing Power ◽  
Dependent Manner ◽  
Paw Edema ◽  
Anti Inflammatory ◽  
In Vitro Antioxidant Activity

Objective: Objective of the present study was to carry out in vivo anti-inflammatory and in vitro antioxidant activity of methanol extract of aerial part of the Blumea eriantha DC belonging to family Asteraceae.Methods: The shade dried aerial part of B. eriantha (0.5 kg) was powdered and extracted with methanol (1.5 x 3L) at room temperature (24h x 3). After filtration combined all the three extracts and were concentrated on rotary evaporator under reduced pressure at 40 °C, thereby providing crude methanol extract which was subsequently employed for further studies. Anti-inflammatory effect was studied by carrageenan-induced paw edema model in rats at dose level 100, 200, and 400 mg/kg. Acute oral toxicity study and in vitro antioxidant potential of the extract was also studied. The in vitro antioxidant activity of methanol extract of aerial part of Blumea eriantha DC was evaluated against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2) and hydroxyl (OH) radicalscavenging and reducing power assays.Results: The results indicate that methanol extract of Blumea eriantha (BEME, 400 mg/kg) exhibited significant inhibition (p<0.001) of increase in paw edema at 5th h. IC50 value of BEME showed significant antioxidant activity. The extract exhibits promising free radical scavenging effect of DPPH, H2O2, OH and reducing power in a dose-dependent manner up to 100µg/ml concentration while the reference standard Ascorbic acid demonstrated more scavenging potential than the methanol extract of Blumea eriantha The methanol extract was found to be safe at the dose of 2000 mg/kg.Conclusion: The results of the experimental study confirmed that methanol extract of Blumea eriantha DC possesses significant anti-inflammatory and antioxidant activity.

scholarly journals Deciphering the Pharmacological Properties of Methanol Extract of Psychotria calocarpa Leaves by In Vivo, In Vitro and In Silico Approaches

2020 ◽  
Vol 13 (8) ◽  
pp. 183 ◽  
Author(s):  
Tahmina Akter Bristy ◽  
Niloy Barua ◽  
Abu Montakim Tareq ◽  
Shahenur Alam Sakib ◽  
Saida Tasnim Etu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
In Silico ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Clot Lysis ◽  
Total Phenol Content ◽  
Molecular Docking Study ◽  
Lysis Activity ◽  
Dose Dependent

The present study explores the neuropharmacological, antinociceptive, antidiarrheal, antioxidant, thrombolytic and cytotoxic activity of methanol extract of Psychotria calocarpa leaves (MEPC). In anxiolytic activity testing of MEPC by elevated plus maze test, hole–board test and light–dark test, the extract exhibited a dose-dependent reduction of anxiety while the open field test observed a decreased locomotion. The administration of MEPC revealed a significant dose-dependent reduction of depressant behavior in forced swimming and tail suspension test. Additionally, the antinociceptive and antidiarrheal activity exposed a significant reduction of nociception and diarrheal behavior at the highest dose. In addition, a strong antioxidant activity was observed in DPPH-free radical-scavenging assay (IC50 = 461.05 μg/mL), total phenol content (118.31 ± 1.12 mg) and total flavonoid content (100.85 ± 0.97 mg). The significant clot–lysis activity was also observed with moderate toxicity (LC50 = 247.92 μg/mL) level in the lethality assay of brine shrimp. Moreover, in silico molecular docking study showed that the compound Psychotriasine could offer promising active site interactions for binding proteins. Furthermore, ADME/T and toxicological properties of the compound satisfied the Lipinski’s rule of five and Veber rules for drug-like potential and toxicity level. Overall, MEPC had a potential neuropharmacological, antinociceptive, antidiarrheal and antioxidant activity that warranted further investigation.

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