scholarly journals Characterization of epithelial elements in human major salivary glands by functional markers: localization of amylase, lactoferrin, lysozyme, secretory component, and secretory immunoglobulins by paired immunofluorescence staining.

1982 ◽  
Vol 30 (7) ◽  
pp. 657-666 ◽  
Author(s):  
F R Korsrud ◽  
P Brandtzaeg
Keyword(s):  
Prolonged Exposure ◽  
Staining Intensity ◽  
Secretory Component ◽  
Immunofluorescence Staining ◽  
Functional Markers ◽  
Cell Periphery ◽  
Tissue Sections ◽  
Collecting Ducts ◽  
Pronase Treatment

Human parotid and submandibular glands were studied by paired immunofluorescence staining, including a variety of combinations of fluorochrome conjugates with contrasting colors. Lactoferrin (Lf), secretory component (SC), and particularly amylase were demonstrated in serous acinar cells of both glands. In addition, lysozyme (Ly) was present in some acini, although mainly located in intercalated ducts where Lf was also most commonly seen. SC was present in acini, intercalated ducts, and striated ducts but not in large collecting ducts. Staining for SC was generally faint but increased in intensity at the cell periphery and particularly at the luminal face of striated duct cells. Immunoglobulin (Ig) A--and IgM when detectable--showed an epithelial distribution similar to that of SC, in accordance with the known secretory properties of these two Ig classes. Conversely, IgG was not present in epithelial cells, despite its high extravascular concentrations. Mucous epithelial elements did not show unequivocal staining for any of the proteins studied. Formaldehyde fixation, combined with pronase treatment of tissue sections and prolonged exposure (20 hr) to antibody, enhanced markedly the staining intensity for lysozyme; ethanol fixation and 30-min incubation with conjugates generally afforded better localization of the other epithelial components.

scholarly journals Semi-Crystalline Hydrophobic Polyamidoamines: A New Family of Technological Materials?

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1018
Author(s):  
Massimo Marcioni ◽  
Jenny Alongi ◽  
Elisabetta Ranucci ◽  
Mario Malinconico ◽  
Paola Laurienzo ◽  
...  
Keyword(s):  
Maximum Stress ◽  
Water Resistance ◽  
Prolonged Exposure ◽  
Water Soluble ◽  
Synthesis And Characterization ◽  
Molecular Weights ◽  
New Family ◽  
Methylene Groups ◽  
Methane Flame

The hitherto known polyamidoamines (PAAs) are not suitable as structural materials because they are usually water-soluble or swellable in water. This paper deals with the synthesis and characterization of semi-crystalline hydrophobic PAAs (H-PAAs) by combining different bis-sec-amines with bis-acrylamides obtained from C6–C12 bis-prim-amines. H-PAAs were initially obtained in a solution of benzyl alcohol, a solvent suitable for both monomers and polymers. Their number average molecular weights, M¯n, which were determined with 1H-NMR by evaluating the percentage of their terminal units, varied from 6000 to >10,000. The solubility, thermal properties, ignitability and water resistance of H-PAAs were determined. They were soluble in organic solvents, semi-crystalline and thermally stable. The most promising ones were also prepared using a bulk process, which has never been previously reported for PAA synthesis. In the form of films, these H-PAAs were apparently unaffected by water. The films underwent tensile and wettability tests. They showed similar Young moduli (260–263 MPa), whereas the maximum stress and the stress at break depended on the number of methylene groups of the starting bis-acrylamides. Their wettability was somewhat higher than that of common Nylons. Interestingly, none of the H-PAAs considered, either as films or powders, ignited after prolonged exposure to a methane flame.

Further Characterization of Storage-Related Alterations in Immunoreactivity of Archival Tissue Sections and its Implications for Collaborative Multicenter Immunohistochemical Studies

2001 ◽  
Vol 9 (3) ◽  
pp. 261-266 ◽  
Author(s):  
E. Oluwabunmi Olapade-Olaopa ◽  
J. Olufemi Ogunbiyi ◽  
E. Hugh MacKay ◽  
Charles A. Muronda ◽  
Temitope O. Alonge ◽  
...  
Keyword(s):  
Archival Tissue ◽  
Tissue Sections ◽  
Immunohistochemical Studies

Differential pathways of recruitment for centrosomal antigens to the mitotic poles during bipolar spindle formation

1991 ◽  
Vol 100 (3) ◽  
pp. 533-540 ◽  
Author(s):  
T. Maekawa ◽  
R. Kuriyama
Keyword(s):  
Drug Treatment ◽  
Single Cell ◽  
Subcellular Distribution ◽  
Immunofluorescence Staining ◽  
Cell Periphery ◽  
Spindle Formation ◽  
Spindle Poles ◽  
Specific Manner ◽  
Bipolar Spindles ◽  
Antibody Localization

As cells enter mitosis, centrosomes undergo many transformations and become associated with different molecules in a stage-specific manner. We have developed a protocol for immunofluorescence staining with four antibody probes that can help us to follow the interaction of centrosomal components during mitosis. The cells were first stained with a human autoimmune serum (5051); a monoclonal anti-phosphocentrosomal antibody (CHO3); and an antitubulin antibody. Localization of the antibodies was detected using rhodamine-, fluorescein- and AMCA-conjugated second antibodies, respectively. After photographing marked mitotic cells, coverslips were soaked with 0.2 M glycine-HCl at pH 1.0 for 1 h to release all antibodies bound to the structures. The same cells were re-stained with a human autoantibody (SP-H) specific for spindle poles and a fluorescein-conjugated second antibody. This allowed us to compare the subcellular distribution of three kinds of centrosomal antigens in a single cell. Mitotic PtK1 cells treated with either nocodazole or taxol included microtubule-containing cytoplasmic foci and parallel bundles of short microtubules at the cell periphery. All the centrosomal antibodies stained the same one or two dots corresponding to structures labeled by the tubulin antibody. CHO3 also revealed extra cytoplasmic foci, whereas the SP-H antigen was additionally localized at one end of the free microtubule bundles. As the microtubules reorganized into bipolar spindles during the recovery from drug treatment, the CHO3 and SP-H antigens coalesced into the spindle poles where the 5051 antigen was located, suggesting that centrosomal antigens become associated with spindle poles through very different recruitment pathways.

Identification and characterization of novel functional markers of EHT

2017 ◽  
Vol 53 ◽  
pp. S121
Author(s):  
Marleen Büchler ◽  
Paul Kaschutnig ◽  
Marleen Büchler ◽  
Roshana Thambyrajah ◽  
Wiebke Nadler ◽  
...  
Keyword(s):  
Functional Markers ◽  
Identification And Characterization

scholarly journals Characterization of antibody against human liver guanase by immunoblotting and immunohistochemical staining of human liver guanase by a direct labeling antibody-enzyme method.

1989 ◽  
Vol 37 (5) ◽  
pp. 611-615 ◽  
Author(s):  
S Ito ◽  
A Iwasaki ◽  
J Syundo ◽  
Y Tamura ◽  
S Kishi ◽  
...  
Keyword(s):  
Liver Enzyme ◽  
Specific Antibody ◽  
Immunohistochemical Staining ◽  
Human Liver ◽  
Liver Extract ◽  
Precipitin Line ◽  
Tissue Sections ◽  
Immunohistochemical Reaction ◽  
Enzyme Method

Human liver guanase was purified and a specific antibody against it was raised in rabbits. The antiserum formed a single precipitin line with human liver extract, and also completely inhibited the activity of the liver enzyme. An immunoblotting study showed that the antibody bound specifically to one band of protein with guanase activity and not to other proteins. Therefore, we concluded that this antiserum against the liver enzyme was suitable for use in immunohistochemical demonstration of guanase. In tissue sections, the immunohistochemical reaction with this antibody was positive in the same locations as the histochemical guanase reaction with DAB (3,3'-diaminobenzidine tetrahydrochloride).

Abstract 1371: Spatially-resolved, multiplexed digital characterization of protein distribution and abundance in FFPE tissue sections

2016 ◽  
Author(s):  
Alessandra Cesano ◽  
Joseph Beechem ◽  
Philippa Webster ◽  
Chris Merritt ◽  
Jaemyeong Jung ◽  
...  
Keyword(s):  
Ffpe Tissue ◽  
Protein Distribution ◽  
Tissue Sections ◽  
Spatially Resolved

scholarly journals Corrosion Inhibitory Effects of Mullite in Concrete Exposed to Sulfuric Acid Attack

2020 ◽  
Vol 1 (2) ◽  
pp. 282-295
Author(s):  
Shima Taheri ◽  
Gerardo Pareja Delgado ◽  
Oluwatoosin B. A. Agbaje ◽  
Paritosh Giri ◽  
Simon Martin Clark
Keyword(s):  
Sulfuric Acid ◽  
Prolonged Exposure ◽  
Acid Corrosion ◽  
Hardness Test ◽  
Aluminum Silicate ◽  
Acid Attack ◽  
Concrete Pipes ◽  
Physicochemical Changes ◽  
Sulfuric Acid Solutions

Prolonged exposure to low pH conditions affects the durability of concrete. In this work, the effect of mullite, aluminum silicate, on the strength and the acid corrosion of mortar and concrete under induced accelerated conditions in sulfuric acid solutions at pH of 0.25 and 1 was studied. The characterization of physicochemical changes was performed using techniques including compressive strength, scanning electron microscopy, micro-X-ray fluorescence spectrometry, and the Vickers hardness test. The results indicate that the addition of mullite does not have any significant effect on the overall strength of mortar and concrete samples, while it significantly increases their resistance to corrosion caused by sulfate attack by 90%, therefore, it is expected to increase the life span and decrease the maintenance costs of concrete pipes subjected to acid corrosion in sewer environments. The inhibition efficiency is observed to be sensitive to acid concentration and was improved with increase in the amount of mullite in samples.

A bioartificial rat heart tissue: Perfusion decellularization and characterization

2019 ◽  
Vol 42 (12) ◽  
pp. 757-764 ◽  
Author(s):  
Busra Ozlu ◽  
Mert Ergin ◽  
Sevcan Budak ◽  
Selcuk Tunali ◽  
Nuh Yildirim ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Tissue Perfusion ◽  
Heart Tissue ◽  
Cell Nuclei ◽  
Tissue Sections ◽  
The Past ◽  
Significant Difference ◽  
Structural Preservation

Despite remarkable advancement in the past decades, heart-related defects are still prone to progress irreversibly and can eventually lead to heart failure. A personalized extracellular matrix–based bioartificial heart created by allografts/xenografts emerges as an alternative as it can retain the original three-dimensional architecture combined with a preserved natural heart extracellular matrix. This study aimed at developing a procedure for decellularizing heart tissue harvested from rats and evaluating decellularization efficiency in terms of residual nuclear content and structural properties. Tissue sections showed no or little visible cell nuclei in decellularized heart, whereas the native heart showed dense cellularity. In addition, there was no significant variation in the alignment of muscle fibers upon decellularization. Furthermore, no significant difference was detected between native and decellularized hearts in terms of fiber diameter. Our findings demonstrate that fiber alignment and diameter can serve as additional parameters in the characterization of biological heart scaffolds as these provide valuable input for evaluating structural preservation of decellularized heart. The bioartificial scaffold formed here can be functionalized with patient’s own material and utilized in regenerative engineering.

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