LacZ staining in paraffin-embedded tissue sections.

Femora and tibiae of rats carrying leukemia from a LacZ-marked acute promyelocytic leukemia-derived leukemic cell line (LT12NL15) were decalcified using EDTA and routinely embedded in paraffin. Sections were used to develop for the first time an immunostaining method for LacZ, employing catalyzed reporter deposition (CARD) based on the deposition of biotinylated tyramine. This method was used to study homing and adhesion of leukemic cells.

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Fluorouracil Selectively Enriches Stem-like Leukemic Cells in a Leukemic Cell Line

International Journal of Biological Sciences ◽

10.7150/ijbs.6.419 ◽

2010 ◽

pp. 419-427 ◽

Cited By ~ 9

Author(s):

Ling Zhang ◽

Song Yang ◽

Yu-Juan He ◽

Hui-Yuan Shao ◽

Li Wang ◽

...

Keyword(s):

Cell Line ◽

Leukemic Cell ◽

Leukemic Cells ◽

Leukemic Cell Line

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Acidic Leucine-Rich Nuclear Phosphoprotein 32 Family Member B (ANP32B), a Novel Caspase-3 Substrate, Exerts Anti-Apoptotic Effects on Acute Myeloid Leukemic Cells.

Blood ◽

10.1182/blood.v112.11.1337.1337 ◽

2008 ◽

Vol 112 (11) ◽

pp. 1337-1337

Author(s):

Yun Yu ◽

Shao-Ming Shen ◽

Li-Shun Wang ◽

Qian Zhao ◽

Guo-Qiang Chen

Keyword(s):

Cell Line ◽

Nuclear Export ◽

Caspase 3 ◽

Expression Profiles ◽

Leukemic Cell ◽

Site Directed Mutagenesis ◽

Leukemic Cells ◽

Leukemic Cell Line ◽

Nuclear Phosphoprotein ◽

Acute Myeloid

Abstract The acidic leucine-rich nuclear phosphoprotein 32B (ANP32B, also called APRIL) is a member of a conserved superfamily of nuclear proteins that includes ANP32A/pp32, a factor that binds histones and inhibits their acetylation and regulates cell growth and differentiation in a tissue-specific manner. Recently, ANP32B was identified as a novel histone chaperone, and it can interact with the transcription factor KLF5, leading to transcriptional repression of a KLF5-downstream gene through stimulation of promoter region-specific histone incorporation and inhibition of histone acetylation. Additionally, ANP32B and/or ANP32A also serve as adaptor molecules linking the HuR nucleocytoplasmic shuttle protein and the nuclear export receptor CRM1 to regulate the cytoplasmic accumulation of some transcripts such as c-fos and CD83. However, its biological activity is still poorly understood. By the two-dimensional electrophoresis plus MALDI-TOF/TOF tandem mass spectrometry-based analysis of subcellular protein expression profiles, we identified ANP32B protein to become a small fragment in the cytosols of apoptotic leukemic cell line induced by NSC606985, a camptothecin analog. The ongoing immunoblot analyses confirmed that ANP32B protein was cleaved during cellular context-independent and caspase-3 activation-dependent apoptosis induced by etoposide, doxorubin and arsenic trioxide besides NSC606985. Further in vitro proteolytic experiments supported that ANP32B is a direct substrate of caspase-3, and the site-directed mutagenesis analysis identified the unclassical aspartate (AEVD163) of ANP32B sequence to be the caspase-3 targeted sites. Thus, we investigated the potential role of ANP32B in apoptosis induction. Our results showed that the suppression of ANP32B expression by siRNA in acute myeloid leukemic cell line U937 cells strongly enhances NSC606985 and etoposide-induced apoptosis. Based on these findings, this work also analyzed molecular mechanism of anti-apoptotic effect of ANP32B, and some interesting findings were confirmed.

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The effect of the leukemic cell line HL60 and acute myeloblastic leukemic cells before and after induction of differentiation on normal pluripotent hematopoietic progenitors (CFU-GEMM)

Leukemia Research ◽

10.1016/0145-2126(85)90003-7 ◽

1985 ◽

Vol 9 (4) ◽

pp. 441-448 ◽

Cited By ~ 3

Author(s):

Rita Michalevicz ◽

Mohammad Reza Taheri ◽

Fay Katz ◽

A.Victor Hoffbrand

Keyword(s):

Cell Line ◽

Leukemic Cell ◽

Leukemic Cells ◽

Hematopoietic Progenitors ◽

Leukemic Cell Line ◽

Before And After ◽

Induction Of Differentiation

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Constitutive expression and role in growth regulation of interleukin-1 and multiple cytokine receptors in a biphenotypic leukemic cell line

Blood ◽

10.1182/blood.v78.1.94.bloodjournal78194 ◽

1991 ◽

Vol 78 (1) ◽

pp. 94-102 ◽

Cited By ~ 1

Author(s):

A Cohen ◽

T Grunberger ◽

W Vanek ◽

ID Dube ◽

PJ Doherty ◽

...

Keyword(s):

Cell Line ◽

Growth Regulation ◽

Interleukin 1 ◽

Constitutive Expression ◽

Leukemic Cell ◽

Leukemic Cells ◽

Cytokine Receptors ◽

Low Density ◽

Leukemic Cell Line ◽

B1 Cells

A cell line (B1) was established from the bone marrow of a patient with a relapse of acute leukemia characterized by a 4;11 chromosomal translocation and biphenotypic features of early B and myeloid lineages. Analysis of the growth requirements of this cell line showed density-dependent growth and secretion of an autostimulatory growth factor, suggesting an autocrine mechanism. Several lines of evidence implicate the participation of interleukin-1 (IL-1) in the autocrine growth regulation of B1 cells. These cells constitutively express the messenger RNA (mRNA) for IL-1 and IL-1 receptor and secrete IL-1; recombinant IL-1 stimulated the growth of colonies when cells were seeded at low density, and anti-IL-1 antibodies inhibited the growth of colonies with cells seeded at higher density. B1 cells do not express detectable levels of mRNA for any of the other cytokines tested, and other cytokines failed to support the growth of B1 cells at low density. In addition, B1 cells express multiple cytokine receptor genes, including the receptors for IL-6, IL-7, tumor necrosis factor and gamma-interferon. Addition of the respective cytokines to the B1 cells resulted in inhibition of the growth of leukemic cells in vitro. The multiplicity of growth-inhibitory cytokine receptors on this leukemic cell line might be due to its biphenotypic lineage and may suggest new therapeutic possibilities in controlling leukemic cell proliferation.

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Purging of acute myeloid leukemic cells by ether lipids and hyperthermia

Blood ◽

10.1182/blood.v72.5.1777.1777 ◽

1988 ◽

Vol 72 (5) ◽

pp. 1777-1783 ◽

Cited By ~ 25

Author(s):

S Okamoto ◽

AC Olson ◽

WE Berdel ◽

WR Vogler

Keyword(s):

Cell Line ◽

Combined Treatment ◽

Leukemic Cell ◽

Leukemic Cells ◽

Ether Lipids ◽

Hematopoietic Progenitors ◽

Leukemic Cell Line ◽

Duration Of Treatment ◽

Acute Myeloid

Abstract Ether lipids (EL) and hyperthermia have been shown to possess a relatively selective cytotoxicity to leukemic cells. In this study, the combined effects of EL (ET-18-OCH3, ET-16-NHCOCH3, or BM 41.440) and hyperthermia on the growth of hematopoietic progenitors, myeloid leukemic cell lines, and leukemic cells obtained from patients with acute myeloid leukemia (AML) were examined to determine if this combination resulted in a greater selective killing of leukemic cells than that achieved by either EL or heat alone. When the cells were treated simultaneously with EL (50 micrograms/mL) and hyperthermia (42 degrees C) for one hour, the killing of leukemic cell line cells was enhanced considerably. Among the three EL, however, the combination of ET-18-OCH3 and heat seemed to be the most cytotoxic to leukemic cell line cells with no effect on the growth of hematopoietic progenitors. An increase in the duration of treatment with ET-18-OCH3 to four hours with heat added during the last hour resulted in a further reduction of leukemic cell line cells while sparing 50% of hematopoietic progenitors after cryopreservation. The combined treatment with ET-18-OCH3 and heat also inhibited the growth of leukemic progenitors obtained from AML patients by 97% to 100%. These data indicate that the combined treatment with EL and hyperthermia might offer an efficient means to eliminate myeloid leukemic cells in vitro.

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Constitutive expression and role in growth regulation of interleukin-1 and multiple cytokine receptors in a biphenotypic leukemic cell line

Blood ◽

10.1182/blood.v78.1.94.94 ◽

1991 ◽

Vol 78 (1) ◽

pp. 94-102 ◽

Cited By ~ 39

Author(s):

A Cohen ◽

T Grunberger ◽

W Vanek ◽

ID Dube ◽

PJ Doherty ◽

...

Keyword(s):

Cell Line ◽

Growth Regulation ◽

Interleukin 1 ◽

Constitutive Expression ◽

Leukemic Cell ◽

Leukemic Cells ◽

Cytokine Receptors ◽

Low Density ◽

Leukemic Cell Line ◽

B1 Cells

Abstract A cell line (B1) was established from the bone marrow of a patient with a relapse of acute leukemia characterized by a 4;11 chromosomal translocation and biphenotypic features of early B and myeloid lineages. Analysis of the growth requirements of this cell line showed density-dependent growth and secretion of an autostimulatory growth factor, suggesting an autocrine mechanism. Several lines of evidence implicate the participation of interleukin-1 (IL-1) in the autocrine growth regulation of B1 cells. These cells constitutively express the messenger RNA (mRNA) for IL-1 and IL-1 receptor and secrete IL-1; recombinant IL-1 stimulated the growth of colonies when cells were seeded at low density, and anti-IL-1 antibodies inhibited the growth of colonies with cells seeded at higher density. B1 cells do not express detectable levels of mRNA for any of the other cytokines tested, and other cytokines failed to support the growth of B1 cells at low density. In addition, B1 cells express multiple cytokine receptor genes, including the receptors for IL-6, IL-7, tumor necrosis factor and gamma-interferon. Addition of the respective cytokines to the B1 cells resulted in inhibition of the growth of leukemic cells in vitro. The multiplicity of growth-inhibitory cytokine receptors on this leukemic cell line might be due to its biphenotypic lineage and may suggest new therapeutic possibilities in controlling leukemic cell proliferation.

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Purging of acute myeloid leukemic cells by ether lipids and hyperthermia

Blood ◽

10.1182/blood.v72.5.1777.bloodjournal7251777 ◽

1988 ◽

Vol 72 (5) ◽

pp. 1777-1783

Author(s):

S Okamoto ◽

AC Olson ◽

WE Berdel ◽

WR Vogler

Keyword(s):

Cell Line ◽

Combined Treatment ◽

Leukemic Cell ◽

Leukemic Cells ◽

Ether Lipids ◽

Hematopoietic Progenitors ◽

Leukemic Cell Line ◽

Duration Of Treatment ◽

Acute Myeloid

Ether lipids (EL) and hyperthermia have been shown to possess a relatively selective cytotoxicity to leukemic cells. In this study, the combined effects of EL (ET-18-OCH3, ET-16-NHCOCH3, or BM 41.440) and hyperthermia on the growth of hematopoietic progenitors, myeloid leukemic cell lines, and leukemic cells obtained from patients with acute myeloid leukemia (AML) were examined to determine if this combination resulted in a greater selective killing of leukemic cells than that achieved by either EL or heat alone. When the cells were treated simultaneously with EL (50 micrograms/mL) and hyperthermia (42 degrees C) for one hour, the killing of leukemic cell line cells was enhanced considerably. Among the three EL, however, the combination of ET-18-OCH3 and heat seemed to be the most cytotoxic to leukemic cell line cells with no effect on the growth of hematopoietic progenitors. An increase in the duration of treatment with ET-18-OCH3 to four hours with heat added during the last hour resulted in a further reduction of leukemic cell line cells while sparing 50% of hematopoietic progenitors after cryopreservation. The combined treatment with ET-18-OCH3 and heat also inhibited the growth of leukemic progenitors obtained from AML patients by 97% to 100%. These data indicate that the combined treatment with EL and hyperthermia might offer an efficient means to eliminate myeloid leukemic cells in vitro.

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