Influence of Cobalt, Sheep Erythropoietin and Several Hormones on Erythropoiesis in Bone Marrows of Isolated Perfused Hind Limbs of Dogs

Abstract A technic has been developed for studying the femoral bone marrow of the dog by perfusing the isolated hind limb. With the use of this method, bone marrows were perfused for 4-6 hours with donor dog blood alone and blood containing sheep erythropoietin, cobalt, growth hormone, hydrocortisone, 3,5,3'-triiodothyronine or testosterone. Significant increases were seen in nucleated erythroid cell counts within femoral marrows perfused with blood containing sheep erythropoietin and cobalt. A slight increase was also noted in the bone marrows from limbs in which growth hormone was added to the perfusion system. It may be concluded from these experiments that erythropoietin, cobalt and growth hormone are capable of stimulating erythroid hyperplasia in the bone marrow directly.

Download Full-text

Acetabular and femoral bone marrow in patients with hip osteoarthrosis: Cellular landscape of immune and stromal compartments

Bone Reports ◽

10.1016/j.bonr.2021.100844 ◽

2021 ◽

Vol 14 ◽

pp. 100844

Author(s):

Drenka Trivanovic ◽

Janek Hader ◽

Maximilian Leucht ◽

Theresa Kreuzahler ◽

Bianca Schlierf ◽

...

Keyword(s):

Bone Marrow ◽

Femoral Bone ◽

Femoral Bone Marrow

Download Full-text

AN EXPERIMENTAL STUDY ON BLOOD FLOW RATE IN THE FEMORAL BONE MARROW CAVITY AFTER PROSTHETIC HIP REPLACEMENT

The KITAKANTO Medical Journal ◽

10.2974/kmj1951.44.645 ◽

1994 ◽

Vol 44 (6) ◽

pp. 645-652

Author(s):

YING MU

Keyword(s):

Bone Marrow ◽

Experimental Study ◽

Blood Flow ◽

Flow Rate ◽

Hip Replacement ◽

Blood Flow Rate ◽

Femoral Bone ◽

Marrow Cavity ◽

Bone Marrow Cavity ◽

Femoral Bone Marrow

Download Full-text

Growth hormone exerts hematopoietic growth-promoting effects in vivo and partially counteracts the myelosuppressive effects of azidothymidine

Blood ◽

10.1182/blood.v80.6.1443.bloodjournal8061443 ◽

1992 ◽

Vol 80 (6) ◽

pp. 1443-1447

Author(s):

WJ Murphy ◽

G Tsarfaty ◽

DL Longo

Keyword(s):

Immune Deficiency ◽

Bone Marrow ◽

Growth Hormone ◽

Progenitor Cell ◽

Hematopoietic Progenitor Cell ◽

Hematopoietic Progenitor ◽

Cell Content ◽

Cell Counts ◽

Growth Promoting

Recombinant human growth hormone (rhGH) was administered to mice to determine its effect on hematopoiesis. BALB/c mice and mice with severe combined immune deficiency (SCID), which lack T cells and B cells, were administered intraperitoneal injections of rhGH for 7 days. Upon analysis, both strains of mice exhibited an increase in splenic and bone marrow hematopoietic progenitor cell content and cellularity, indicating that rhGH can act as a hematopoietic growth factor. C57BL/6 mice were then placed on azidothymidine (AZT). AZT is a reverse transcriptase inhibitor currently used as a treatment for acquired immune deficiency syndrome (AIDS), but which also produces significant myelotoxic effects. Treatment of mice with rhGH partially counteracted the myelosuppressive properties of AZT. Bone marrow cellularity, hematocrit values, white blood cell counts, and splenic hematopoietic progenitor cell content were all significantly increased if rhGH (20 micrograms injected intraperitoneally every other day) was concurrently administered with AZT. Administration of ovine GH (ovGH), which, unlike rhGH, has no effect on murine prolactin receptors, also prevented the erythroid-suppressive effects of AZT in mice, but had no significant effect on granulocyte counts. Thus, the effects of GH are mediated at least in part through GH receptors in vivo. Additionally, when mice were initially myelosuppressed by several weeks of AZT treatment, the subsequent administration of ovGH resulted in an increase in splenic hematopoietic progenitor cells. No significant pathologic effects were observed in mice receiving either repeated rhGH or ovGH injections. Thus, GH exerts significant direct hematopoietic growth-promoting effects in vivo and may be of potential clinical use to promote hematopoiesis in the face of myelotoxic therapy.

Download Full-text

Total Cell Counts of the Bone Marrow of Normal Albino Rats from 1 to 50 Weeks of Age

Blood ◽

10.1182/blood.v14.4.409.409 ◽

1959 ◽

Vol 14 (4) ◽

pp. 409-414 ◽

Cited By ~ 15

Author(s):

WILLIAM T. BURKE ◽

CHARLES HARRIS

Keyword(s):

Bone Marrow ◽

Cell Population ◽

Normal Values ◽

Age Groups ◽

Considerable Change ◽

Total Cell ◽

Albino Rats ◽

Cell Counts ◽

Total Cell Population ◽

Femoral Bone Marrow

Abstract A method is described by which the total nucleated cell count of femoral bone marrow of the rat can be estimated and cell population expressed in terms of differential counts. Normal values of total nucleated cell counts and the cellular distributions are given for seven age groups. These data indicate considerable change in bone marrow total cell population in rats one to 10 weeks of age.

Download Full-text

FEMORAL BONE MARROW BIOPSY IN THE ALBINO RAT

Blood ◽

10.1182/blood.v3.3.292.292 ◽

1948 ◽

Vol 3 (3) ◽

pp. 292-294 ◽

Cited By ~ 7

Author(s):

D. G. CAMERON ◽

G. M. WATSON

Keyword(s):

Bone Marrow ◽

Bone Marrow Biopsy ◽

Femoral Bone ◽

Albino Rat ◽

Femoral Bone Marrow ◽

Differential Counts

Abstract A technic for repeated femoral bone marrow biopsy in the rat is detailed. Differential counts confirmed the distribution of the cellular elements as described by other authors.

Download Full-text

Tritium retention in femoral bone marrow and spleens of mice receiving single intravenous injections of tritiated water and tritiated thymidine.

Journal of Radiation Research ◽

10.1269/jrr.28.204 ◽

1987 ◽

Vol 28 (3) ◽

pp. 204-212

Author(s):

HISAMASA JOSHIMA ◽

SATORU MATSUSHITA ◽

KUMIKO FUKUTSU ◽

MASATOSHI KASHIMA

Keyword(s):

Bone Marrow ◽

Tritiated Thymidine ◽

Tritiated Water ◽

Femoral Bone ◽

Intravenous Injections ◽

Femoral Bone Marrow

Download Full-text

Qnantitation of Femoral Bone Marrow Cellularity of Rats with Acute Chloroleukemia

Blood ◽

10.1182/blood.v26.3.309.309 ◽

1965 ◽

Vol 26 (3) ◽

pp. 309-316 ◽

Cited By ~ 3

Author(s):

EVELYN E. VARSA ◽

EUGENE S. HANDLER ◽

ALBERT S. GORDON

Keyword(s):

Bone Marrow ◽

Leukemic Cells ◽

Femoral Bone ◽

Progressive Decrease ◽

Cell Numbers ◽

Bone Marrow Cellularity ◽

Acute Form ◽

Long Evans ◽

Marrow Cellularity ◽

Femoral Bone Marrow

Abstract Procedures of bone marrow quantitation have been applied to the study of the pathogenesis of a leukemia in rats. Mature Long-Evans rats developed an acute form of the Shay Chloroleukemia after intravenous administration of leukemic cells. Assessment of total nucleated cell numbers (normal and leukemic) per mg. of femoral bone marrow was made during the course of the pathogenesis (20 days). Reductions in the numbers of normal marrow elements were observed prior to significant increases in chloroleukemic cells. A progressive decrease in total marrow cellularity was noted in all subsequent stages. In animals surviving 17-20 days, the total number of hemic cells in the femoral marrow was found to be approximately 40 per cent of that seen in normal animals. The leukemia developed more rapidly in young than in adult animals. Using total and differential bone marrow cellularity as a criterion for the stage of pathogenesis, standardized leukemic rats can be prepared for experimentation.

Download Full-text