scholarly journals Brief Note: Marrow Repopulating Ability of Peripheral Blood Cells Compared to Thoracic Duct Cells

Blood ◽  
1968 ◽  
Vol 32 (4) ◽  
pp. 662-667 ◽  
Author(s):  
R. STORB ◽  
R. B. EPSTEIN ◽  
E. D. THOMAS
Keyword(s):  
Bone Marrow ◽  
Thoracic Duct ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Whole Body ◽  
Thoracic Duct Lymph ◽  
Peripheral Blood Cells ◽  
Hemopoietic Stem Cells ◽  
Duct Lymph ◽  
Marrow Repopulating Ability

Abstract Ten dogs were exposed to 1200 r. of whole body irradiation at a dose rate of 9.2 r./min. Five of these dogs were then given infusions of 21 to 74 x 109 autologous peripheral blood cells which had been previously stored at -80 C. 4.0 to 19.4 x 109 of these cells were lymphocytes, 0.4 to 4.9 x 109 were monocytes and 16.4 to 50.3 x 109 were granulocytes. All five dogs showed clinical or histologic evidence of bone marrow repopulation. The remaining 5 dogs were given 7 to 22 x 109 autologous thoracic duct lymphocytes. In none of these dogs was marrow repopulation observed. It was concluded that hemopoietic stem cells are not present in the thoracic duct lymph of the dog in any appreciable number.

scholarly journals MECHANISM OF EOSINOPHILIA

1970 ◽  
Vol 131 (6) ◽  
pp. 1288-1305 ◽  
Author(s):  
Antony Basten ◽  
Paul B. Beeson
Keyword(s):  
Bone Marrow ◽  
Thoracic Duct ◽  
Peripheral Blood ◽  
Bone Marrow Cells ◽  
Thoracic Duct Lymph ◽  
Antilymphocyte Serum ◽  
Diffusion Chambers ◽  
Duct Lymph ◽  
Diffusible Factor ◽  
Secondary Type

A possible role for the lymphocyte in the mechanism of eosinopoiesis has been examined. Procedures known to deplete or inactivate the pool of recirculating lymphocytes such as neonatal thymectomy, administration of antilymphocyte serum, and prolonged thoracic duct drainage, either singly or in combination, resulted in a highly significant reduction in the eosinophil response to trichinosis. Irradiated animals exposed to parasitic challenge did not develop eosinophilia unless reconstituted with lymphocytes as well as bone marrow cells. When "memory" cells were used instead of normal lymphocytes, a "secondary" type of eosinophil response was observed. Transfer of a primary eosinophilia was achieved adoptively with a population of living large lymphocytes from thoracic duct lymph and peripheral blood, but not with blood plasma or cell-free lymph. The potency of the active lymphocytes was not impaired by enclosing them in cell-tight diffusion chambers, indicating that they exerted an effect on bone marrow by agency of a diffusible factor. The demonstration of a role for lymphocytes in induction of the eosinophil response to this kind of stimulus supports the conclusion that eosinophilia belongs in the category of immunologic phenomena.

Transplantation of Bone Marrow as Compared with Peripheral-Blood Cells from HLA-Identical Relatives in Patients with Hematologic Cancers

2001 ◽  
Vol 344 (3) ◽  
pp. 175-181 ◽  
Author(s):  
William I. Bensinger ◽  
Paul J. Martin ◽  
Barry Storer ◽  
Reginald Clift ◽  
Steven J. Forman ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Cells

scholarly journals Bone Marrow and Peripheral Blood Cells Toxicity of a Single 2.0 Gy Cobalt60 Ionizing Radiation: An Animal Model

1970 ◽  
Vol 29 (2) ◽  
Author(s):  
Shittu Akeem ◽  
Olatunbosun Lukman ◽  
Khalil Eltahir ◽  
Olalere Fatai ◽  
Babatunde Abiola ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Marrow Cell ◽  
P Value ◽  
Peripheral Blood Cells ◽  
P Values ◽  
Cell Parameters ◽  
Post Irradiation ◽  
Total Body

BACKGROUND: Bone marrow is extremely vulnerable to damage caused by radiation therapy. Hence, bone marrow suppression is an important side effect of radiotherapy. Effective use of radiotherapy is therefore compromised by radiation-related injuries.MATERIAL AND METHODS: Six Guinea-pigs were recruited for the study of which three were subjected to total body irradiation with Co60 while the other three served as controls. Bone marrow and peripheral blood samples were collected before and at days 9, 14 and 21, post irradiation. Manual and automated counts were performed for bone marrow nucleated cells and peripheral blood cells respectively.RESULTS: Declining bone marrow cellularity was evident immediately post irradiation. Mean ± SD of marrow cell counted per mm3 were 121,924±281, 87,603±772, 121,367±375 and122,750±1000 pre-irradiation and days 9, 14 and 21, postirradiation (p-values 0.10, 0.27 and 0.29 respectively). Significant drops in counts were noticed on day 9 post-irradiation for all red cell parameters (p-values <0.05), for Total White Blood Cell Count and Neutrophil count (p-values <0.05) and also on days 14 and 21 for Lymphocytes (p-values <0.05) and on day 21 for Eosinophil/Basophil/Monocytes (p-value <0.05). A significant drop in platelets counts was also noticed on day 9 (p-value <0.05) which significantly increased above pre-irradiation value on day 21.CONCLUSION: Total body irrradiation with Co60 significantly affects the bone marrow with maximum reductions in marrow nucleated cells and peripheral blood cells counts on day 9 post irradiation. 

scholarly journals Chromosome Studies in Normal and Leukemic Rats

Blood ◽  
1964 ◽  
Vol 23 (5) ◽  
pp. 564-571 ◽  
Author(s):  
G. DOWD ◽  
K. DUNN ◽  
WILLIAM C. MOLONEY
Keyword(s):  
Bone Marrow ◽  
Blood Cell ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Species Differences ◽  
Peripheral Blood Cell ◽  
Myelogenous Leukemia ◽  
Peripheral Blood Cells ◽  
X Ray ◽  
Normal Rat

Abstract 1. Adequate chromosome preparations were obtained in 70 per cent of normal rat peripheral blood cell cultures. However, cultures of peripheral blood cells from leukemic rats were almost universally unsuccessful. 2. In x-ray- and 3MCA-induced leukemias direct bone marrow preparations provided adequate metaphases in eight of 12 cases. Failures were attributed in four cases to scanty material obtained from fibrotic marrows. 3. No consistent chromosome abnormalities, such as those reported in human myelogenous leukemia, were found in these leukemic rats. However, the series of cases is small, and species differences and other factors may have influenced the results of these studies.

scholarly journals LYSOZYME IN BONE MARROW AND PERIPHERAL BLOOD CELLS

1966 ◽  
Vol 14 (2) ◽  
pp. 167-170 ◽  
Author(s):  
ROBERT S. BRIGGS ◽  
PASQUALE E. PERILLIE ◽  
STUART C. FINCH
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Plasma Cells ◽  
Lysozyme Activity ◽  
Peripheral Blood Cells ◽  
Histochemical Technique

By means of an indirect histochemical technique, the intracellular lysozyme of the formed elements of the peripheral blood and bone marrow was estimated. Evidence is presented that monocytes, as well as mature neutrophils and their precursors extending back to the progranulocyte, contain significant amounts of this enzyme. A rare mature eosinophil demonstrated a trace of lysozyme activity. There was no evidence of lysozyme activity in basophils, erythrocytes, megakaryocytes, platelets, plasma cells, tissue mast cells or bone marrow reticuloendothelial cells.

The distribution of HLA on human lymphoid, bone marrow and peripheral blood cells

1979 ◽  
Vol 9 (4) ◽  
pp. 272-275 ◽  
Author(s):  
Geoffrey Brown ◽  
Peter Biberfeld ◽  
Birger Christensson ◽  
David Y. Mason
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Cells
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