A simple technique for preparation of young red cells for transfusion from ordinary blood units

Abstract We have developed a rapid and simple method for the isolation of relatively young cells from ordinary fresh or previously frozen blood units, using the IBM-2991 Blood Cell Processor. Based on in vitro measurements of red cell pyruvate kinase activity, the resolution of the method is excellent, comparable to that of the stractan discontinuous gradient system. In splenectomized patients with thalassemia major, the administration of the “youngest” 50% of blood units yielded 51Cr survival times of 40 and 42 days for fresh packed red cells and 41 and 43 days for previously frozen red cells.

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A simple technique for preparation of young red cells for transfusion from ordinary blood units

Blood ◽

10.1182/blood.v59.4.865.bloodjournal594865 ◽

1982 ◽

Vol 59 (4) ◽

pp. 865-868

Author(s):

JH Graziano ◽

S Piomelli ◽

C Seaman ◽

T Wang ◽

AR Cohen ◽

...

Keyword(s):

Simple Technique ◽

Thalassemia Major ◽

Red Cells ◽

Gradient System ◽

Cell Processor ◽

Survival Times ◽

Pyruvate Kinase Activity ◽

Simple Method ◽

In Vitro Measurements

We have developed a rapid and simple method for the isolation of relatively young cells from ordinary fresh or previously frozen blood units, using the IBM-2991 Blood Cell Processor. Based on in vitro measurements of red cell pyruvate kinase activity, the resolution of the method is excellent, comparable to that of the stractan discontinuous gradient system. In splenectomized patients with thalassemia major, the administration of the “youngest” 50% of blood units yielded 51Cr survival times of 40 and 42 days for fresh packed red cells and 41 and 43 days for previously frozen red cells.

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A Dynamic State in Stored Erythrocytes as Reflected by the Undulating Nature of in Vitro Measurements

Blood ◽

10.1182/blood.v9.4.362.362 ◽

1954 ◽

Vol 9 (4) ◽

pp. 362-373

Author(s):

ELIZABETH H. THIELE ◽

ROBERT B. PENNELL

Keyword(s):

Seasonal Variation ◽

Cholinesterase Activity ◽

The State ◽

Red Cells ◽

Dynamic State ◽

In Vitro Measurements

Abstract The fluctuating capacity of stored erythrocytes to release gas when suspended in the presence of dextrose in Krebs-Ringer bicarbonate solution in an atmosphere of 5 per cent CO2-95 per cent N2 is demonstrated. A not dissimilar fluctuation of the cholinesterase activity of the stored cells is reported. Possible seasonal variation in the state of red cells is suggested. Some implications of these observations for erythrocyte storage are suggested.

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A RAPID AND SIMPLE METHOD FOR THE ISOLATION AND CULTURE OF LEUKOCYTES FOR CHROMOSOME ANALYSIS IN DOMESTIC ANIMALS

Canadian Journal of Animal Science ◽

10.4141/cjas76-004 ◽

1976 ◽

Vol 56 (1) ◽

pp. 27-31 ◽

Cited By ~ 11

Author(s):

C. C. LIN ◽

D. R. NEWTON ◽

W. K. SMINK ◽

R. B. CHURCH

Keyword(s):

Reproductive Performance ◽

Whole Blood ◽

Simple Technique ◽

Chromosome Analysis ◽

Domestic Animals ◽

Chromosome Preparation ◽

Gradient System ◽

Simple Method ◽

Screening Programs ◽

Routine Manner

Leukocytes of cattle, sheep and swine can be quickly and easily isolated from heparinized whole blood using a 6% Ficoll–10% Hypaque gradient system. The leukocytes isolated using this method are easily cultured in a routine manner for chromosome preparation. It was found that both good quality and quantity of metaphase spreads can be consistently obtained from all the animals examined. This simple technique will facilitate cytogenetic screening programs in the bovine and swine where chromosome abnormalities seem to be associated with reproductive performance.

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In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651281 ◽

1968 ◽

Vol 20 (03/04) ◽

pp. 384-396 ◽

Cited By ~ 1

Author(s):

G Zbinden ◽

S Tomlin

Keyword(s):

Platelet Adhesion ◽

Sodium Citrate ◽

Blood Platelets ◽

In Vitro Assay ◽

Red Cells ◽

Platelet Adhesiveness ◽

Vitro Assay ◽

In Vitro System ◽

Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

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The Filter Loop Technique as a Method of Measuring Platelet Aggregation in the Flowing Blood of the Rat; the Inhibitory Activity of 5-oxo-l-cyclopentene-l-heptanoic Acid (AY-16,804) on Platelet Aggregation

Thrombosis and Haemostasis ◽

10.1055/s-0038-1649110 ◽

1973 ◽

Vol 30 (01) ◽

pp. 138-147 ◽

Cited By ~ 1

Author(s):

Christopher R. Muirhead

Keyword(s):

Platelet Aggregation ◽

Prostaglandin E1 ◽

Suitable Model ◽

Heptanoic Acid ◽

Simple Method ◽

Loop Technique ◽

Flowing Blood ◽

Filter Loop

SummaryThe filter loop technique which measures platelet aggregation in vivo in the flowing-blood of the rat was compared to the optical density technique of Born which is carried out in vitro with platelet rich plasma. Using these two experimental models the effect on platelet aggregation of three known inhibitors sulfinpyrazone, dipyridamole and prostaglandin E1, and a novel compound 5-oxo-l-cyclopentene-l-heptanoic acid (AY-16, 804) was determined.The effects on platelet aggregation of the known inhibitors were consistent with information in the literature. Prostaglandin E1 was the most potent inhibitor in both techniques; sulfinpyrazone inhibited aggregation in both models but was less potent than prostaglandin E1. AY-16, 804 exhibited activity in vitro and in vivo similar to that of sulfinpyrazone. Dipyridamole did not inhibit platelet aggregation in vivo and did not inhibit aggregation in vitro in concentrations at which it remained soluble.The filter loop technique is a suitable model for measuring platelet aggregation in the flowing blood of the rat. It is a relatively simple method of determining aggregation and easily adapted to other species.

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LACK OF EFFECT OF CORTICOSTEROIDS ON THE IN VIVO DISAPPEARANCE OF SULFHYDRYL-INHIBITED AND ANTIBODY-COATED ERYTHROCYTES

Acta Endocrinologica ◽

10.1530/acta.0.047s028 ◽

1964 ◽

Vol 47 (3_Suppl) ◽

pp. S28-S36

Author(s):

Kailash N. Agarwal

Keyword(s):

Red Cells ◽

List Type ◽

Red Cell

ABSTRACT Red cells were incubated in vitro with sulfhydryl inhibitors and Rhantibody with and without prior incubation with prednisolone-hemisuccinate. These erythrocytes were labelled with Cr51 and P32 and their disappearance in vivo after autotransfusion was measured. Prior incubation with prednisolone-hemisuccinate had no effect on the rate of red cell disappearance. The disappearance of the cells was shown to take place without appreciable intravascular destruction.

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Morphological Changes and Iron Uptake in Human Normoblasts in Vitro: I. Proliferation and Destruction of Nucelated Red Cells During Incubation of Normal Bone Marrow

Scandinavian Journal of Clinical and Laboratory Investigation ◽

10.3109/00365516409060509 ◽

1964 ◽

Vol 16 (2) ◽

pp. 222-232 ◽

Cited By ~ 3

Author(s):

Erik Myhre

Keyword(s):

Bone Marrow ◽

Iron Uptake ◽

Morphological Changes ◽

Red Cells ◽

Normal Bone Marrow ◽

Normal Bone

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Rhenium-188 labeled recombinant human plasminogen kringle5 (rhk5) and preliminary biodistribution

Nuklearmedizin ◽

10.3413/nukmed-0349-10-09 ◽

2011 ◽

Vol 50 (06) ◽

pp. 234-239 ◽

Cited By ~ 3

Author(s):

R. Guo ◽

Y. Ma ◽

R. Zhang ◽

S. Liang ◽

H. Shen ◽

...

Keyword(s):

Lung Adenocarcinoma ◽

Human Serum ◽

Critical Role ◽

Angiogenesis Inhibitor ◽

Simple Method ◽

Tumour Formation ◽

Human Plasminogen ◽

Specificity Of Binding ◽

A549 Lung

Summary Aim: Angiogenesis plays a critical role in tumour formation and metastasis. Suitable radiolabeled angiogenesis inhibitor can be used for noninvasive imaging of angiogenesis and radionuclide therapy. Here we prepare rhenium-188 labeled recombinant human plasminogen kringle5 (188Re-rhk5) in a convenient manner than evaluate its properties in A549 lung adenocarcinoma. Methods: 188Rerhk5 was obtained by conjugating His group at the C end of rhk5 with fac- [188Re(H2O)3(CO)3]+. Chelating efficiency of fac-[188Re(H2O)3(CO)3]+ and radiolabeling efficiency of 188Re-rhk5 were measured by radio thin-layer chromatography (RTLC). In vitro stability of 188Re-rhk5 was determined in human serum at 37°C and analyzed by RTLC. Competition test was also performed to verify the specificity of binding. A biodistribution study was carried out in nude mice bearing A549 lung adenocarcinoma. Results: 188Rerhk5 was obtained with a radiolabel efficiency of 66.1%, the radiochemical purity (RCP) can marreach 95.2% after purification. 188Re-rhk5 showed high stability in human serum, the RCP was more than 80% even 12 h after incubation. Competition test showed a high binding specificity. Furthermore, this radio-complex was excreted mainly through kidneys and showed specific tumour uptake in mice bearing A549 tumours. Conclusion: 188Re-rhk5 was prepared by a simple method. Preliminary biodistribution results showed its potential as an agent for possible tumour imaging, therapy and encouraged further investigation.

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