Hemin-induced conversion of pyruvate kinase isozymes in K562 cells
Keyword(s):
The effects of hemin on the conversion of pyruvate kinase (PK) isozymes from the M2-type to the L-type in K562 cells were investigated. Immunofluorescence, ion exchange chromatography, and electrophoretic studies showed that the untreated K562 cells contained only the M2-type PK, while eight to 20 days after induction with hemin, concomitant with hemoglobin F synthesis, L-type PK levels increased while M2-type PK levels decreased. Electrophoretic study revealed three hybrid isozymes of the L-type and M2-type PK. We conclude that the conversion of PK isozymes from the M2-type to the L-type in erythroid precursor cells occurs in the early stage of maturation.
1973 ◽
Vol 30
(02)
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pp. 414-424
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1982 ◽
Vol 42
(1)
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pp. 27-33
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2018 ◽
Vol 20
(1)
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pp. 56-60
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1965 ◽
Vol 30
(7)
◽
pp. 2361-2367
1965 ◽
Vol 30
(7)
◽
pp. 2351-2360
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