scholarly journals Regulation of human peripheral blood erythroid burst-forming unit growth by T lymphocytes and T lymphocyte subpopulations defined by OKT4 and OKT8 monoclonal antibodies

Blood ◽  
1985 ◽  
Vol 65 (2) ◽  
pp. 456-463
Author(s):  
D Wisniewski ◽  
A Strife ◽  
M Wachter ◽  
B Clarkson
Keyword(s):  
Monoclonal Antibodies ◽  
T Lymphocytes ◽  
T Lymphocyte ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Sheep Erythrocyte ◽  
Cell Fraction ◽  
Null Cell ◽  
Cell Subpopulations ◽  
T Cell Subpopulations

To reexamine the influence that T lymphocytes have on the regulation of human peripheral blood burst-forming unit (BFU-E) proliferation in the absence of a statistically significant number of monocytes, very low numbers (3 to 10 X 10(3)/mL) of a null cell fraction highly enriched for BFU-E were cultured alone and in the presence of 5 X 10(5) sheep erythrocyte-purified, autologous T lymphocytes in a methylcellulose culture system containing erythropoietin. T lymphocytes consistently enhanced the growth of BFU-E from the null cell fraction, as reflected in both their number and size. Irradiation of T lymphocytes prior to coculture with null cells markedly reduced this enhancement, strongly suggesting that T lymphocytes synthesize erythroid burst-promoting factors (BPA). To determine whether there were functional differences between the two major T lymphocyte populations as defined by OKT4 (T helper/inducer) and OKT8 (T suppressor/cytotoxic) murine monoclonal antibodies to stimulate the growth of BFU-E, both T cell subpopulations were isolated by negative (panning) or positive (fluorescence-activated cell sorting) selection and cocultured with null cells. No statistically significant differences emerged between unseparated, OKT4+ and OKT8+ T lymphocytes in their ability to stimulate the growth of BFU-E. Thus, these studies provide further evidence that T lymphocytes are a major population of BPA-producing cells and further that OKT4+ and OKT8+ T lymphocytes equally elaborate these factors.

scholarly journals Regulation of human peripheral blood erythroid burst-forming unit growth by T lymphocytes and T lymphocyte subpopulations defined by OKT4 and OKT8 monoclonal antibodies

Blood ◽  
1985 ◽  
Vol 65 (2) ◽  
pp. 456-463 ◽  
Author(s):  
D Wisniewski ◽  
A Strife ◽  
M Wachter ◽  
B Clarkson
Keyword(s):  
Monoclonal Antibodies ◽  
T Lymphocytes ◽  
T Lymphocyte ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Sheep Erythrocyte ◽  
Cell Fraction ◽  
Null Cell ◽  
Cell Subpopulations ◽  
T Cell Subpopulations

Abstract To reexamine the influence that T lymphocytes have on the regulation of human peripheral blood burst-forming unit (BFU-E) proliferation in the absence of a statistically significant number of monocytes, very low numbers (3 to 10 X 10(3)/mL) of a null cell fraction highly enriched for BFU-E were cultured alone and in the presence of 5 X 10(5) sheep erythrocyte-purified, autologous T lymphocytes in a methylcellulose culture system containing erythropoietin. T lymphocytes consistently enhanced the growth of BFU-E from the null cell fraction, as reflected in both their number and size. Irradiation of T lymphocytes prior to coculture with null cells markedly reduced this enhancement, strongly suggesting that T lymphocytes synthesize erythroid burst-promoting factors (BPA). To determine whether there were functional differences between the two major T lymphocyte populations as defined by OKT4 (T helper/inducer) and OKT8 (T suppressor/cytotoxic) murine monoclonal antibodies to stimulate the growth of BFU-E, both T cell subpopulations were isolated by negative (panning) or positive (fluorescence-activated cell sorting) selection and cocultured with null cells. No statistically significant differences emerged between unseparated, OKT4+ and OKT8+ T lymphocytes in their ability to stimulate the growth of BFU-E. Thus, these studies provide further evidence that T lymphocytes are a major population of BPA-producing cells and further that OKT4+ and OKT8+ T lymphocytes equally elaborate these factors.

scholarly journals Purification and characterization of granulocytic progenitor cells (CFU- C) from human peripheral blood using immunologic surface markers

Blood ◽  
1978 ◽  
Vol 51 (1) ◽  
pp. 1-8 ◽  
Author(s):  
CM Richman ◽  
L Chess ◽  
RA Yankee
Keyword(s):  
Progenitor Cells ◽  
Peripheral Blood ◽  
B Lymphocyte ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Sheep Erythrocyte ◽  
Methyl Cellulose ◽  
Null Cell ◽  
Peripheral Blood Mononuclear

Abstract The concentration of committed granulocytic progenitor cells (CFU-C) in functionally unique subpopulations of human peripheral blood mononuclear cells has been determined by the in vitro methyl-cellulose assay. Using immunoabsorbent column chromatography and rosette-depletion techniques, we have demonstrated that CFU-C, although not present in either purified T or B lymphocyte populations, are highly concentrated in the “null” cell population, which lacks sheep erythrocyte receptors and surface immunoglobulin. Further fractionation of this null subset has demonstrated that CFU-C do not bear complement receptors, but require the presence of peripheral blood mononuclear cell feeder layers for maximum proliferation.

Effect of Qigong Training on Proportions of T Lymphocyte Subsets in Human Peripheral Blood

1995 ◽  
Vol 23 (01) ◽  
pp. 27-36 ◽  
Author(s):  
Hoon Ryu ◽  
Chang Duk Jun ◽  
Bok Soo Lee ◽  
Byung Min Choi ◽  
Hyung Min Kim ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
T Lymphocyte ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Human Peripheral Blood ◽  
Absolute Number ◽  
T Lymphocyte Subsets ◽  
Healthy Group ◽  
Cd8 T Lymphocytes ◽  
Trainee Group

The effect of Qigong training on proportions of T lymphocyte subsets was investigated in human peripheral blood. We observed that the ratio of CD4+/CD8+ T lymphocytes was increased as much as 50% in a trainee group who practiced Qigong training more than 5 months compared to a normal healthy group who did not practice. The absolute number of CD4+ T lymphocytes was also elevated in trainee group with 100 cells/mm 3 more than in normal healthy group. The positive correlation between the ratio of CD4+/CD8+ T lymphocytes and the ratio of CD4+45RA-/CD4+ CD45RA+ T lymphocytes was shown in the trainee group. In contrast, there was a negative correlation between the ratio of CD4+/CD8+ T lymphocytes and the ratio of CD8+CD57+/CD8+CD57- T lymphocytes in the trainee group. The data indicate that Qigong training affects the profile of lymphocyte subsets in human peripheral blood, especially the proportion of CD4+ T lymphocytes.

scholarly journals Fractionation of human T lymphocytes on wheat germ agglutinin-sepharose.

1976 ◽  
Vol 144 (5) ◽  
pp. 1381-1385 ◽  
Author(s):  
U Hellström ◽  
M L Dillner ◽  
S Hammarström ◽  
P Perlmann
Keyword(s):  
T Cells ◽  
Phaseolus Vulgaris ◽  
Concanavalin A ◽  
Peripheral Blood ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Human Peripheral Blood ◽  
Fluorescein Isothiocyanate ◽  
Cell Subpopulations ◽  
T Cell Subpopulations

T cells from human peripheral blood was purified by fractionation on columns charged with human immunoglobulin and rabbit anti-human immuno-globulin. When assayed with 125I- or fluorescein isothiocyanate-labeled wheat-germ agglutinin (WGA), a weakly binding and a strongly binding subpopulation could be distinguished. These T-cell subpopulations were fractionated on columns charged with WGA, convalently bound to Sepharose 6MB. The cells responding to the mitogens leukoagglutinin from Phaseolus vulgaris and concanavalin A were enriched in the strongly binding subpopulation (approximately 20% of the T cells) while they were depleted from the weakly binding subpopulation.

scholarly journals Differential expression of somatostatin receptor subtypes in human peripheral blood mononuclear cell subsets

2004 ◽  
pp. 565-577 ◽  
Author(s):  
EG Lichtenauer-Kaligis ◽  
VA Dalm ◽  
SP Oomen ◽  
DM Mooij ◽  
PM van Hagen ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Mrna Expression ◽  
T Lymphocyte ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Lymphocyte Activation ◽  
Cell Types ◽  
Facs Analysis ◽  
Peripheral Blood Mononuclear ◽  
B And T Lymphocytes

BACKGROUND: Somatostatin (SS)-binding sites have been demonstrated in human lymphoid tissues and peripheral blood cells. However, not much is known with respect to the SS receptor subtype (sst) expression pattern and the expression of SS itself in the immune system. OBJECTIVE: The aim of this study was to evaluate the mRNA expression of the five known sst (sst(1-5)) in peripheral blood mononuclear cell (sub)populations. Moreover, the expression of the mRNAs encoding SS and the SS-like peptide cortistatin (CST) in immune cell subsets was studied. METHODS: RT-PCR and quantitative PCR were performed to evaluate sst, SS and CST mRNA expression in cells in the basal or activated state. Fluorescence-activated cell sorter (FACS) analysis using fluorescent SS was performed to visualize sst protein on cell membranes. RESULTS: B- and T-lymphocytes selectively expressed sst(3) mRNA. sst(3) expression in B-lymphocytes was significantly lower compared with T-lymphocytes. Unstimulated, freshly isolated monocytes did not express any sst mRNA. Upon activation, monocytes selectively expressed sst(2) mRNA, whereas T-lymphocyte activation upregulated sst(3) expression. sst(2) mRNA expression on monocytes was confirmed by FACS analysis. B- and T-lymphocytes did not express SS mRNA, while both cell types expressed CST mRNA. CST mRNA expression was downregulated following T-lymphocyte activation. CONCLUSION: We demonstrate for the first time unequivocally that human peripheral blood B- and T-lymphocytes selectively express sst(3), whereas monocytes do not express sst. However, upon activation, monocytes are induced to express sst(2A). No expression of SS mRNA was detected in any cell type, whereas all cell types expressed CST mRNA. The differential expression of sst and CST mRNA in lymphocytes and monocytes suggests a functional significance for the CST-sst interaction in immune cells, but further studies should be performed to evaluate the significance of sst and CST in these cells.

scholarly journals Purification and characterization of granulocytic progenitor cells (CFU- C) from human peripheral blood using immunologic surface markers

Blood ◽  
1978 ◽  
Vol 51 (1) ◽  
pp. 1-8
Author(s):  
CM Richman ◽  
L Chess ◽  
RA Yankee
Keyword(s):  
Progenitor Cells ◽  
Peripheral Blood ◽  
B Lymphocyte ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Sheep Erythrocyte ◽  
Methyl Cellulose ◽  
Null Cell ◽  
Peripheral Blood Mononuclear

The concentration of committed granulocytic progenitor cells (CFU-C) in functionally unique subpopulations of human peripheral blood mononuclear cells has been determined by the in vitro methyl-cellulose assay. Using immunoabsorbent column chromatography and rosette-depletion techniques, we have demonstrated that CFU-C, although not present in either purified T or B lymphocyte populations, are highly concentrated in the “null” cell population, which lacks sheep erythrocyte receptors and surface immunoglobulin. Further fractionation of this null subset has demonstrated that CFU-C do not bear complement receptors, but require the presence of peripheral blood mononuclear cell feeder layers for maximum proliferation.

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