Induction of nuclear factor kB/Rel nuclear activity in human peripheral blood T lymphocytes by anti-HLA class I monoclonal antibodies

1997 ◽  
Vol 50 (1) ◽  
pp. 1-7 ◽  
Author(s):  
M. C. Turco ◽  
M. F. Romano ◽  
A. Lamberti ◽  
A. Petrella ◽  
R. Bisogni ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
T Lymphocytes ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Hla Class I ◽  
Class I ◽  
Nuclear Factor ◽  
Nuclear Activity ◽  
Nuclear Factor Kb

scholarly journals Regulation of human peripheral blood erythroid burst-forming unit growth by T lymphocytes and T lymphocyte subpopulations defined by OKT4 and OKT8 monoclonal antibodies

Blood ◽  
1985 ◽  
Vol 65 (2) ◽  
pp. 456-463
Author(s):  
D Wisniewski ◽  
A Strife ◽  
M Wachter ◽  
B Clarkson
Keyword(s):  
Monoclonal Antibodies ◽  
T Lymphocytes ◽  
T Lymphocyte ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Sheep Erythrocyte ◽  
Cell Fraction ◽  
Null Cell ◽  
Cell Subpopulations ◽  
T Cell Subpopulations

To reexamine the influence that T lymphocytes have on the regulation of human peripheral blood burst-forming unit (BFU-E) proliferation in the absence of a statistically significant number of monocytes, very low numbers (3 to 10 X 10(3)/mL) of a null cell fraction highly enriched for BFU-E were cultured alone and in the presence of 5 X 10(5) sheep erythrocyte-purified, autologous T lymphocytes in a methylcellulose culture system containing erythropoietin. T lymphocytes consistently enhanced the growth of BFU-E from the null cell fraction, as reflected in both their number and size. Irradiation of T lymphocytes prior to coculture with null cells markedly reduced this enhancement, strongly suggesting that T lymphocytes synthesize erythroid burst-promoting factors (BPA). To determine whether there were functional differences between the two major T lymphocyte populations as defined by OKT4 (T helper/inducer) and OKT8 (T suppressor/cytotoxic) murine monoclonal antibodies to stimulate the growth of BFU-E, both T cell subpopulations were isolated by negative (panning) or positive (fluorescence-activated cell sorting) selection and cocultured with null cells. No statistically significant differences emerged between unseparated, OKT4+ and OKT8+ T lymphocytes in their ability to stimulate the growth of BFU-E. Thus, these studies provide further evidence that T lymphocytes are a major population of BPA-producing cells and further that OKT4+ and OKT8+ T lymphocytes equally elaborate these factors.

Anti HLA class I monoclonal antibody effect on PKC kinetics in PHA activated human peripheral blood mononuclear and E4 cells

1988 ◽  
Vol 156 (1) ◽  
pp. 46-53 ◽  
Author(s):  
Licia Poltronieri ◽  
Edon Melloni ◽  
Michele Rubini ◽  
Rita Selvatici ◽  
Cristina Mazzilli ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Hla Class I ◽  
Class I ◽  
Peripheral Blood Mononuclear

scholarly journals Regulation of human peripheral blood erythroid burst-forming unit growth by T lymphocytes and T lymphocyte subpopulations defined by OKT4 and OKT8 monoclonal antibodies

Blood ◽  
1985 ◽  
Vol 65 (2) ◽  
pp. 456-463 ◽  
Author(s):  
D Wisniewski ◽  
A Strife ◽  
M Wachter ◽  
B Clarkson
Keyword(s):  
Monoclonal Antibodies ◽  
T Lymphocytes ◽  
T Lymphocyte ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Sheep Erythrocyte ◽  
Cell Fraction ◽  
Null Cell ◽  
Cell Subpopulations ◽  
T Cell Subpopulations

Abstract To reexamine the influence that T lymphocytes have on the regulation of human peripheral blood burst-forming unit (BFU-E) proliferation in the absence of a statistically significant number of monocytes, very low numbers (3 to 10 X 10(3)/mL) of a null cell fraction highly enriched for BFU-E were cultured alone and in the presence of 5 X 10(5) sheep erythrocyte-purified, autologous T lymphocytes in a methylcellulose culture system containing erythropoietin. T lymphocytes consistently enhanced the growth of BFU-E from the null cell fraction, as reflected in both their number and size. Irradiation of T lymphocytes prior to coculture with null cells markedly reduced this enhancement, strongly suggesting that T lymphocytes synthesize erythroid burst-promoting factors (BPA). To determine whether there were functional differences between the two major T lymphocyte populations as defined by OKT4 (T helper/inducer) and OKT8 (T suppressor/cytotoxic) murine monoclonal antibodies to stimulate the growth of BFU-E, both T cell subpopulations were isolated by negative (panning) or positive (fluorescence-activated cell sorting) selection and cocultured with null cells. No statistically significant differences emerged between unseparated, OKT4+ and OKT8+ T lymphocytes in their ability to stimulate the growth of BFU-E. Thus, these studies provide further evidence that T lymphocytes are a major population of BPA-producing cells and further that OKT4+ and OKT8+ T lymphocytes equally elaborate these factors.

scholarly journals Diacylglycerol activates the influx of extracellular cations in T-lymphocytes independently of intracellular calcium-store depletion and possibly involving endogenous TRP6 gene products

2002 ◽  
Vol 364 (1) ◽  
pp. 245-254 ◽  
Author(s):  
Alessandra GAMBERUCCI ◽  
Emanuele GIURISATO ◽  
Paola PIZZO ◽  
Maristella TASSI ◽  
Roberta GIUNTI ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
T Lymphocytes ◽  
Peripheral Blood ◽  
Transient Receptor Potential ◽  
Human Peripheral Blood ◽  
Receptor Potential ◽  
Animal Cells ◽  
Bivalent Cation ◽  
Intracellular Calcium Store ◽  
Transient Receptor

In Jurkat and human peripheral blood T-lymphocytes, 1-oleoyl-2-acetyl-sn-glycerol (OAG), a membrane-permeant analogue of diacylglycerol, activated the influx of Ca2+, Ba2+ and Sr2+. OAG also caused plasma-membrane depolarization in Ca2+-free media that was recovered by the addition of bivalent cation, indicating the activation of Na+ influx. OAG-induced cation influx was (i) mimicked by the natural dacylglycerol 1-stearoyl-2-arachidonyl-sn-glycerol, (ii) not blocked by inhibiting protein kinase C or in the absence of phopholipase C activity and (iii) blocked by La3+ and Gd3+. Differently from OAG, both thapsigargin and phytohaemagglutinin activated a potent influx of Ca2+, but little influx of Ba2+ and Sr2+. Moreover, the influx of Ca2+ activated by thapsigargin and that activated by OAG were additive. Furthermore, several drugs (i.e. econazole, SKF96365, carbonyl cyanide p-trifluoromethoxyphenylhydrazone, 2-aminoethoxy diphenylborate and calyculin-A), while inhibiting the influx of Ca2+ induced by both thapsigargin and phytohaemagglutinin, did not affect OAG-stimulated cation influx. Transient receptor potential (TRP) 3 and TRP6 proteins have been shown previously to be activated by diacylglycerol when expressed heterologously in animal cells [Hofmann, Obukhov, Schaefer, Harteneck, Gudermann and Schultz (1999) Nature (London) 397, 259–263]. In both Jurkat and peripheral blood T-lymphocytes, mRNA encoding TRP proteins 1, 3, 4 and 6 was detected by reverse transcriptase PCR, and the TRP6 protein was detected by Western blotting in a purified plasma-membrane fraction. We conclude that T-cells express a diacylglycerol-activated cation channel, unrelated to the channel involved in capacitative Ca2+ entry, and associated with the expression of TRP6 protein.

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