scholarly journals Ektacytometric measurement of sickle cell deformability as a continuous function of oxygen tension [published erratum appears in Blood 1987 Apr;69(4):1272]

Blood ◽  
1987 ◽  
Vol 69 (1) ◽  
pp. 316-323
Author(s):  
MP Sorette ◽  
MG Lavenant ◽  
MR Clark
Keyword(s):  
Oxygen Saturation ◽  
Oxygen Tension ◽  
Sickle Cell ◽  
Hemoglobin Concentration ◽  
Exchange System ◽  
Cell Deformability ◽  
Linear Gradient ◽  
Couette Viscometer ◽  
Gas Exchange System

In an effort to study the rheologic effects of small amounts of hemoglobin S (HbS) polymer in sickle red cells, we have used the ektacytometer, a laser diffraction couette viscometer, to measure sickle cell deformability as a function of oxygen tension. Sickle cell populations of defined intracellular hemoglobin concentration (MCHC) were isolated using Stractan density gradients and were resuspended in buffered polyvinylpyrrolidone solutions for deformability measurements. Using a gas-porous, hollow fiber gas exchange system to establish a linear gradient in oxygen tension, deformability was measured over a pO2 range of 76 to 0 mm Hg. Parallel spectroscopic determinations of oxygen saturation permitted determination of cell deformability as a function of oxygen saturation for each discrete MCHC population. From these measurements the level of oxygen saturation at which a loss in cell deformability was first detected could be defined. Then, using the data of Noguchi and Schecter, the amount of polymerized HbS in the cells at that defined level of oxygen saturation was estimated. The results of this analysis suggested that the quantity of polymer that caused a detectable loss in cell deformability increased with increasing MCHC. In addition, for MCHC above 30 g/dL, this represented a substantial fraction of the total HbS in the cell.

scholarly journals Ektacytometric measurement of sickle cell deformability as a continuous function of oxygen tension [published erratum appears in Blood 1987 Apr;69(4):1272]

Blood ◽  
1987 ◽  
Vol 69 (1) ◽  
pp. 316-323 ◽  
Author(s):  
MP Sorette ◽  
MG Lavenant ◽  
MR Clark
Keyword(s):  
Oxygen Saturation ◽  
Oxygen Tension ◽  
Sickle Cell ◽  
Hemoglobin Concentration ◽  
Exchange System ◽  
Cell Deformability ◽  
Linear Gradient ◽  
Couette Viscometer ◽  
Gas Exchange System

Abstract In an effort to study the rheologic effects of small amounts of hemoglobin S (HbS) polymer in sickle red cells, we have used the ektacytometer, a laser diffraction couette viscometer, to measure sickle cell deformability as a function of oxygen tension. Sickle cell populations of defined intracellular hemoglobin concentration (MCHC) were isolated using Stractan density gradients and were resuspended in buffered polyvinylpyrrolidone solutions for deformability measurements. Using a gas-porous, hollow fiber gas exchange system to establish a linear gradient in oxygen tension, deformability was measured over a pO2 range of 76 to 0 mm Hg. Parallel spectroscopic determinations of oxygen saturation permitted determination of cell deformability as a function of oxygen saturation for each discrete MCHC population. From these measurements the level of oxygen saturation at which a loss in cell deformability was first detected could be defined. Then, using the data of Noguchi and Schecter, the amount of polymerized HbS in the cells at that defined level of oxygen saturation was estimated. The results of this analysis suggested that the quantity of polymer that caused a detectable loss in cell deformability increased with increasing MCHC. In addition, for MCHC above 30 g/dL, this represented a substantial fraction of the total HbS in the cell.

Nocturnal oxygen saturation and painful sickle cell crises in children

Blood ◽  
2003 ◽  
Vol 101 (3) ◽  
pp. 846-848 ◽  
Author(s):  
Darren R. Hargrave ◽  
Angie Wade ◽  
Jane P. M. Evans ◽  
Deborah K. M. Hewes ◽  
Fenella J. Kirkham
Keyword(s):  
Oxygen Saturation ◽  
Sickle Cell ◽  
Clinical Laboratory ◽  
Upper Airway ◽  
Fetal Hemoglobin ◽  
Hemoglobin Concentration ◽  
Study Data ◽  
Sleep Study ◽  
Multiple Regression Modeling ◽  
Painful Crisis

Abstract The pathogenesis of acute painful crisis in children with sickle cell disease is poorly understood; suggested risk factors include sickle cell type, severity of anemia, fetal hemoglobin concentration, and hypoxemia from upper airway obstruction. In a cohort study of 95 patients the relationship between clinical, laboratory, and sleep study data and frequency of painful crisis was investigated. Both univariate and multiple regression modeling showed that low nocturnal oxygen saturation was highly significantly associated with a higher rate of painful crisis in childhood (P < .0001). Screening and treatment for hypoxemia may reduce the frequency of this and other complications of the disease.

scholarly journals The incidence of painful crisis in homozygous sickle cell disease: correlation with red cell deformability

Blood ◽  
1988 ◽  
Vol 72 (6) ◽  
pp. 2056-2059
Author(s):  
WM Lande ◽  
DL Andrews ◽  
MR Clark ◽  
NV Braham ◽  
DM Black ◽  
...  
Keyword(s):  
Sickle Cell ◽  
Hemoglobin Concentration ◽  
Red Cells ◽  
Strong Positive Correlation ◽  
Red Cell ◽  
Cell Disease ◽  
Cell Deformability ◽  
Red Cell Deformability ◽  
Cellular Dehydration ◽  
Painful Crisis

To determine whether the vasoocclusive severity of homozygous sickle cell (SS) disease is influenced by cellular dehydration, we correlated the incidence of painful crisis with steady-state measurements of red cell hydration. Sixteen children with SS disease were followed for 3.3 to 8 years (mean, 6.8 years), and a single crisis rate was calculated for each patient. At the time of well visits, cellular hydration was assessed by measuring cell deformability, the percentage of red cells with a density greater than or equal to 1.1056 g/mL, and the percentage of irreversibly sickled cells (ISC). The incidence of painful crisis showed a strong positive correlation with Omax, a deformability measurement reflecting cellular hydration (r = .84, P less than .002), and with hemoglobin concentration (r = .59, P = .04). That is, higher crisis rates were observed in patients with less dehydrated, more deformable red cells and also in patients with higher hemoglobin concentrations. Furthermore, cell deformability and hemoglobin concentration were independent predictors of the incidence of painful crisis, which is consistent with separate effects of these two red cells parameters on vasoocclusive severity.

scholarly journals The incidence of painful crisis in homozygous sickle cell disease: correlation with red cell deformability

Blood ◽  
1988 ◽  
Vol 72 (6) ◽  
pp. 2056-2059 ◽  
Author(s):  
WM Lande ◽  
DL Andrews ◽  
MR Clark ◽  
NV Braham ◽  
DM Black ◽  
...  
Keyword(s):  
Sickle Cell ◽  
Hemoglobin Concentration ◽  
Red Cells ◽  
Strong Positive Correlation ◽  
Red Cell ◽  
Cell Disease ◽  
Cell Deformability ◽  
Red Cell Deformability ◽  
Cellular Dehydration ◽  
Painful Crisis

Abstract To determine whether the vasoocclusive severity of homozygous sickle cell (SS) disease is influenced by cellular dehydration, we correlated the incidence of painful crisis with steady-state measurements of red cell hydration. Sixteen children with SS disease were followed for 3.3 to 8 years (mean, 6.8 years), and a single crisis rate was calculated for each patient. At the time of well visits, cellular hydration was assessed by measuring cell deformability, the percentage of red cells with a density greater than or equal to 1.1056 g/mL, and the percentage of irreversibly sickled cells (ISC). The incidence of painful crisis showed a strong positive correlation with Omax, a deformability measurement reflecting cellular hydration (r = .84, P less than .002), and with hemoglobin concentration (r = .59, P = .04). That is, higher crisis rates were observed in patients with less dehydrated, more deformable red cells and also in patients with higher hemoglobin concentrations. Furthermore, cell deformability and hemoglobin concentration were independent predictors of the incidence of painful crisis, which is consistent with separate effects of these two red cells parameters on vasoocclusive severity.

scholarly journals Reliability of Low-Cost Near-Infrared Spectroscopy in the Determination of Muscular Oxygen Saturation and Hemoglobin Concentration during Rest, Isometric and Dynamic Strength Activity

2020 ◽  
Vol 17 (23) ◽  
pp. 8824
Author(s):  
Claudia Miranda-Fuentes ◽  
Isabel María Guisado-Requena ◽  
Pedro Delgado-Floody ◽  
Leonidas Arias-Poblete ◽  
Alejandro Pérez-Castilla ◽  
...  
Keyword(s):  
Oxygen Saturation ◽  
Near Infrared ◽  
Low Cost ◽  
Intraclass Correlation ◽  
Dynamic Strength ◽  
Hemoglobin Concentration ◽  
Isometric Strength ◽  
Near Infrared Reflectance ◽  
Initial Load

Background: The objective of this study was to establish the reliability of the Humon Hex near-infrared reflectance spectroscopy (NIRS) in determining muscle oxygen saturation (SmO2) and hemoglobin concentration (Hgb) at rest and during isometric and dynamic strength exercises using a functional electromechanical dynamometer (FEMD). Methods: The SmO2 and Hgb values of sixteen healthy adults (mean ± standard deviation (SD): Age = 36.1 ± 6.4 years) were recorded at rest and during isometry (8 s), dynamic strength I (initial load of 40% of the average isometric load, with 2 kg increments until muscle failure) and dynamic strength II (same as I, but with an initial load of 40% of the maximum isometric load) activity. To evaluate the reliability in the determination of SmO2 and Hgb of this device, intraclass correlation coefficient (ICC), standard error of measurement (SEM) and coefficient of variation (CV) were obtained. Results: The main results obtained are SmO2 at rest (CV = 5.76%, SEM = 3.81, ICC = 0.90), isometric strength (CV = 3.03%, SEM = 2.08, ICC = 0.92), dynamic strength I (CV = 10.6, SEM = 7.17, ICC = 0.22) and dynamic strength II (CV = 9.69, SEM = 6.75, ICC = 0.32); Hgb at rest (CV = 1.97%, SEM = 0.24, ICC = 0.65), isometric strength (CV = 0.98%, SEM = 0.12, ICC = 0.96), dynamic strength I (CV = 3.25, SEM = 0.40, ICC = 0.54) and dynamic strength II (CV = 2.74, SEM = 0.34, ICC = 0.65). Conclusions: The study shows that Humon Hex is a reliable device to obtain SmO2 and Hgb data in healthy adult subjects at rest and during isometric strength, providing precision for measurements made with this device.

NOTES ON THE APPLICATION OF THE HERSCH GALVANIC OXYGEN CELL TO MEASUREMENT OF BLOOD OXYGEN CONTENT AND TENSION

1961 ◽  
Vol 39 (6) ◽  
pp. 991-999 ◽  
Author(s):  
D. V. Bates ◽  
E. V. Harkness
Keyword(s):  
Standard Deviation ◽  
Oxygen Saturation ◽  
Oxygen Content ◽  
Oxygen Tension ◽  
Blood Oxygen ◽  
Average Volume ◽  
Blood Samples ◽  
Factors Influencing ◽  
The Mean

A number of galvanic oxygen cells have been built to the design of Hersch (1). Factors influencing the sensitivity of this type of cell to oxygen have been studied, and the potentialities of the cells in the analysis of small gas samples have been explored. With the cell used in the manner described, analysis of 105 samples of air of an average volume of 0.001 ml showed the standard deviation of the percentage of oxygen to be 0.9%. The mean oxygen percentage of these samples was correct at 20.8%. These cells were found to be suitable for the analysis of the oxygen contained in very small blood samples, and the method described probably offers the most precise technique there is for the determination of the oxygen saturation of 0.002 ml of blood. By bubble equilibration techniques, the cell may also be used to determine the oxygen tension of blood samples in the range 0–200 mm Hg.

scholarly journals SPECTROPHOTOMETRIC DETERMINATION OF THE OXYGEN SATURATION OF WHOLE BLOOD

1955 ◽  
Vol 217 (1) ◽  
pp. 479-488
Author(s):  
Makepeace U. Tsao ◽  
Shirley S. Sethna ◽  
Charles H. Sloan ◽  
Lillian J. Wyngarden
Keyword(s):  
Oxygen Saturation ◽  
Spectrophotometric Determination ◽  
Whole Blood

scholarly journals Inaccuracy in automated measurement of hematocrit and corpuscular indices in the presence of severe hyperglycemia

Blood ◽  
1981 ◽  
Vol 57 (6) ◽  
pp. 1065-1067 ◽  
Author(s):  
JA Strauchen ◽  
W Alston ◽  
J Anderson ◽  
Z Gustafson ◽  
LF Fajardo
Keyword(s):  
Blood Glucose ◽  
Mean Corpuscular Volume ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Volume ◽  
Automated Measurement ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Hyperosmolar Glucose

Abstract Because we recently observed two patients with severe diabetic hyperglycemia and spuriously elevated electronically determined hematocrit and mean corpuscular volume (MCV), we investigated the effect of hyperglycemia on two popular automated hematology systems, the Coulter S and Ortho ELT-8. Marked hyperglycemia (blood glucose 800-- 2000 mg/dl) caused consistent overestimation of the electronically determined MCV compared to that derived from a simultaneous spun microhematocrit. The resultant overestimation and underestimation, respectively, of the derived values for hematocrit and mean corpuscular hemoglobin concentration may be clinically misleading. The mechanism of MCV elevation in hyperglycemia appears to be swelling of hyperosmolar glucose “loaded” erythrocytes when diluted into “isotonic” counting medium. This effect is readily circumvented by determination of a spun microhematocrit.

Effect of Age on Oxygen-Binding in Normal Human Subjects

1976 ◽  
Vol 51 (2) ◽  
pp. 185-188
Author(s):  
P. M. Tweeddale ◽  
R. J. E. Leggett ◽  
D. C. Flenley
Keyword(s):  
Oxygen Saturation ◽  
Oxygen Tension ◽  
Inorganic Phosphate ◽  
Human Subjects ◽  
Oxygen Binding ◽  
Corpuscular Haemoglobin ◽  
Positive Correlation ◽  
Normal Human ◽  
Mean Corpuscular Haemoglobin ◽  
Effect Of Age

1. Oxygen-binding, plasma and intra-erythrocytic pH, and haemoglobin, 2,3-diphosphoglycerate and inorganic phosphate concentrations were measured in sixty-two healthy non-smokers aged between 18 and 89 years. 2. P50 (oxygen tension at 50% oxygen saturation) expressed at plasma pH 7·40 and Pco2 5·33 kPa showed a positive correlation with age. 3. This correlation of P50 with age was closer when P50 was expressed at a constant intra-erythrocytic pH 7·20. On average P50 at intra-erythrocytic pH 7·20 increased from 3·59 kPa at 20 years to 3·96 kPa at 90 years of age. 4. 2,3-Diphosphoglycerate, inorganic phosphate, haemoglobin and mean corpuscular haemoglobin concentrations did not correlate with P50 or with age.

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