scholarly journals Expression of c-fos, c-myb, and c-myc in human monocytes: correlation with monocytic differentiation

Blood ◽  
1987 ◽  
Vol 69 (5) ◽  
pp. 1542-1545
Author(s):  
J Lee ◽  
K Mehta ◽  
MB Blick ◽  
JU Gutterman ◽  
G Lopez-Berestein
Keyword(s):  
Human Peripheral Blood ◽  
Terminal Differentiation ◽  
Functional Differentiation ◽  
Leukemia Cells ◽  
Blood Monocytes ◽  
Monocytic Differentiation ◽  
Monocytic Leukemia ◽  
Peripheral Blood Monocytes ◽  
Human Monocytic Leukemia ◽  
Monocytic Lineage

Terminal differentiation of human monocytic leukemia cells (THP-1 cells) was associated with the induction of c-fos, the down regulation of c-myb, and no significant change in the level of c-myc expression. Gamma interferon, which resulted in a slight decrease in c-myb but no change in c-fos or c-myc expression, had a transient antiproliferative effect without a morphological or functional differentiation of THP-1 cells. Resting human peripheral blood monocytes have a high c-fos, a low c-myc, and no detectable c-myb expression. These findings suggest that a switch in c-fos/c-myb expression is associated with the terminal differentiation of cells of the monocytic lineage.

scholarly journals Expression of c-fos, c-myb, and c-myc in human monocytes: correlation with monocytic differentiation

Blood ◽  
1987 ◽  
Vol 69 (5) ◽  
pp. 1542-1545 ◽  
Author(s):  
J Lee ◽  
K Mehta ◽  
MB Blick ◽  
JU Gutterman ◽  
G Lopez-Berestein
Keyword(s):  
Human Peripheral Blood ◽  
Terminal Differentiation ◽  
Functional Differentiation ◽  
Leukemia Cells ◽  
Blood Monocytes ◽  
Monocytic Differentiation ◽  
Monocytic Leukemia ◽  
Peripheral Blood Monocytes ◽  
Human Monocytic Leukemia ◽  
Monocytic Lineage

Abstract Terminal differentiation of human monocytic leukemia cells (THP-1 cells) was associated with the induction of c-fos, the down regulation of c-myb, and no significant change in the level of c-myc expression. Gamma interferon, which resulted in a slight decrease in c-myb but no change in c-fos or c-myc expression, had a transient antiproliferative effect without a morphological or functional differentiation of THP-1 cells. Resting human peripheral blood monocytes have a high c-fos, a low c-myc, and no detectable c-myb expression. These findings suggest that a switch in c-fos/c-myb expression is associated with the terminal differentiation of cells of the monocytic lineage.

scholarly journals Flavonoid 4′-O-Methylkuwanon E fromMorus albaInduces the Differentiation of THP-1 Human Leukemia Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Peter Kollar ◽  
Tomáš Bárta ◽  
Stanislava Keltošová ◽  
Pavlína Trnová ◽  
Veronika Müller Závalová ◽  
...  
Keyword(s):  
Surface Antigen ◽  
Leukemia Cells ◽  
Human Leukemia ◽  
Monocytic Differentiation ◽  
Monocytic Leukemia ◽  
Nbt Reduction ◽  
Dependent Growth ◽  
White Mulberry ◽  
Cell Growth And Viability ◽  
Human Monocytic Leukemia

Aims. In this work we studied cytodifferentiation effects of newly characterized prenyl flavonoid 4′-O-methylkuwanon E (4ME) isolated from white mulberry (Morus albaL.).Main Methods. Cell growth and viability were measured by dye exclusion assay; cell cycle and surface antigen CD11b were monitored by flow cytometry. For the cytodifferentiation of cells the NBT reduction assay was employed. Regulatory proteins were assessed by western blotting.Key Findings. 4ME induced dose-dependent growth inhibition of THP-1 cells, which was not accompanied by toxic effect. Inhibition of cells proliferation caused by 4ME was associated with the accumulation in G1 phase and with downregulation of hyperphosphorylated pRb. Treatment with 4ME led to significant induction of NBT-reducing activity of PMA stimulated THP-1 cells and upregulation expression of differentiation-associated surface antigen CD11b. Our results suggest that monocytic differentiation induced by 4ME is connected with up-regulation of p38 kinase activity.Significance. Our study provides the first evidence that 4ME induces the differentiation of THP-1 human monocytic leukemia cells and thus is a potential cytodifferentiating anticancer agent.

scholarly journals Association of the expression of an SR-cyclophilin with myeloid cell differentiation

Blood ◽  
1996 ◽  
Vol 87 (6) ◽  
pp. 2269-2274 ◽  
Author(s):  
SL Giardina ◽  
JD Coffman ◽  
HA Young ◽  
SJ Potter ◽  
JL Frey ◽  
...  
Keyword(s):  
Antisense Rna ◽  
Human Peripheral Blood ◽  
Myeloid Cell ◽  
Blood Monocytes ◽  
Monocytic Differentiation ◽  
Myristate Acetate ◽  
Peripheral Blood Monocytes ◽  
Monocyte Differentiation ◽  
Kinetics Of

The role of a 150-kD SR-cyclophilin (NK-TR1) in monocyte differentiation was investigated. Using an antipeptide monoclonal antibody, we have detected NK-TR1 in human peripheral blood monocytes and HL-60 cells. Unstimulated monocytes showed a low intracellular level of NK-TR1 protein that increased over 3 days of lipopolysaccharide + interferon-gamma treatment, consistent with the kinetics of monocyte differentiation. Normal HL-60 cells also had a low level of NK-TR1 protein, and exposure to 1.25% dimethyl sulfoxide (DMSO) resulted in a marked transient increase in expression that returned to basal levels before the development of granulocyte differentiation-associated biochemical changes. Phorbol myristate acetate, a promoter of monocytic differentiation in HL-60 cells, also caused a significant increase in NK-TR1 over basal levels. Transfection of a vector expressing NK-TR1 antisense RNA into HL-60 cells suppressed DMSO-mediated growth arrest. In addition, the development of a more mature phenotype, as measured by expression of CD16, and the ability to reduce nitroblue tetrazoleum dye was inhibited in transfectants when compared with controls. These results are consistent with the hypothesis that the NK-TR1 gene product is required for the progression towards a mature differentiated phenotype.

scholarly journals Identification of RANTES receptors on human monocytic cells: competition for binding and desensitization by homologous chemotactic cytokines.

1993 ◽  
Vol 177 (3) ◽  
pp. 699-705 ◽  
Author(s):  
J M Wang ◽  
D W McVicar ◽  
J J Oppenheim ◽  
D J Kelvin
Keyword(s):  
Human Peripheral Blood ◽  
Leukemia Cell ◽  
Calcium Mobilization ◽  
Leukemia Cell Line ◽  
Blood Monocytes ◽  
Monocytic Leukemia ◽  
Monocytic Cells ◽  
Inflammatory Protein ◽  
Binding Data ◽  
Human Monocytic Leukemia

RANTES (regulated on activation, normal T expressed and secreted) is a member of the chemotactic cytokine (chemokine) beta subfamily. High affinity receptors for RANTES have been identified on a human monocytic leukemia cell line THP-1, which responded to RANTES in chemotaxis and calcium mobilization assays. Steady-state binding data analyses revealed approximately 700 binding sites/cell on THP-1 cells with a Kd value of 400 pM, comparable to that expressed on human peripheral blood monocytes. The RANTES binding to monocytic cells was competed for by monocyte chemotactic and activating factor (MCAF) and macrophage inflammatory protein 1 (MIP-1) alpha, two other chemokine beta cytokines. Although MCAF and MIP-1 alpha competed for RANTES binding to monocytes with apparent lower affinity (with estimated Kd of 6 and 1.6, nM respectively) both of these cytokines effectively desensitized the calcium mobilization induced by RANTES. The chemotactic response of THP-1 cells to RANTES was also markedly inhibited by preincubation with MCAF or MIP-1 alpha. In contrast, RANTES did not desensitize the THP-1 calcium mobilization and chemotaxis in response to MCAF or MIP-1 alpha. These results, together with our previous observations that RANTES did not compete for MCAF or MIP-1 alpha binding on monocytic cells, indicate the expression of promiscuous receptors on monocytes that recognize one or more cytokines within the chemokine beta family.

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