scholarly journals Human neutrophil-mediated lysis of ovarian cancer cells

Blood ◽  
1989 ◽  
Vol 74 (2) ◽  
pp. 805-809 ◽  
Author(s):  
A Lichtenstein ◽  
M Seelig ◽  
J Berek ◽  
J Zighelboim
Keyword(s):  
Ovarian Cancer ◽  
Tumor Cells ◽  
Human Tumor ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Myristate Acetate ◽  
Human Tumor Cells ◽  
Effector Cells ◽  
Lethal Event ◽  
Stimulation Of

Abstract Because of recent questions concerning the sensitivity of human tumor cells to neutrophil-induced oxidative injury, we studied six freshly obtained human ovarian cancer (OC) specimens. Stimulation of neutrophils (PMNs) by phorbol myristate acetate (PMA) did not result in OC cytolysis during the first nine hours of incubation. However, three of six specimens were significantly lysed by stimulated PMNs when assay length was increased to 18 hours. Cytotoxicity was mediated by PMN production of reactive oxidative intermediates (ROIs). Presentation of ROIs to OC targets as preformed or enzymatically generated molecules in cell-free systems duplicated the enhanced lysis at 18 hours (as compared with six hours). Since addition of catalase at three or six hours did not inhibit enhanced lysis at 18 hours (achieved by PMNs or in cell-free systems), it appears that an initial ROI-mediated lethal event occurs early, but longer incubations are required for the event to become manifested as cell death. These data suggest that shorter assays may underestimate the potential of PMNs as effector cells against human tumor cells.

scholarly journals Human neutrophil-mediated lysis of ovarian cancer cells

Blood ◽  
1989 ◽  
Vol 74 (2) ◽  
pp. 805-809
Author(s):  
A Lichtenstein ◽  
M Seelig ◽  
J Berek ◽  
J Zighelboim
Keyword(s):  
Ovarian Cancer ◽  
Tumor Cells ◽  
Human Tumor ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Myristate Acetate ◽  
Human Tumor Cells ◽  
Effector Cells ◽  
Lethal Event ◽  
Stimulation Of

Because of recent questions concerning the sensitivity of human tumor cells to neutrophil-induced oxidative injury, we studied six freshly obtained human ovarian cancer (OC) specimens. Stimulation of neutrophils (PMNs) by phorbol myristate acetate (PMA) did not result in OC cytolysis during the first nine hours of incubation. However, three of six specimens were significantly lysed by stimulated PMNs when assay length was increased to 18 hours. Cytotoxicity was mediated by PMN production of reactive oxidative intermediates (ROIs). Presentation of ROIs to OC targets as preformed or enzymatically generated molecules in cell-free systems duplicated the enhanced lysis at 18 hours (as compared with six hours). Since addition of catalase at three or six hours did not inhibit enhanced lysis at 18 hours (achieved by PMNs or in cell-free systems), it appears that an initial ROI-mediated lethal event occurs early, but longer incubations are required for the event to become manifested as cell death. These data suggest that shorter assays may underestimate the potential of PMNs as effector cells against human tumor cells.

Growth Factor-Dependent Stimulation of Primary Human Tumor Cells

1990 ◽  
pp. 173-180
Author(s):  
H. Depenbrock ◽  
J. Trijssenaar ◽  
D. Neukam ◽  
H.-J. Meyer ◽  
M. Freund ◽  
...  
Keyword(s):  
Growth Factor ◽  
Tumor Cells ◽  
Human Tumor ◽  
Human Tumor Cells ◽  
Stimulation Of

Early stimulation of acidification rate by novel cytotoxic pyridyl cyanoguanidines in human tumor cells: comparison with m-Iodobenzylguanidine

2000 ◽  
Vol 60 (6) ◽  
pp. 839-849 ◽  
Author(s):  
Sara Ekelund ◽  
Gunnar Liminga ◽  
Fredrik Björkling ◽  
Erik Ottosen ◽  
Charlotte Schou ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Human Tumor Cells ◽  
Early Stimulation ◽  
Stimulation Of

Methods for the Study of Cell-Mediated Immunological Response against Human Tumor Cells Cultured in Vitro

1970 ◽  
Vol 56 (5) ◽  
pp. 291-296
Author(s):  
Giuseppe Fossati ◽  
Maria I. Colnaghi
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Immunological Response ◽  
Human Tumor Cells ◽  
Effector Cells ◽  
Cytotoxic Test ◽  
Specific Antigens ◽  
Technical Details ◽  
Automatic Reading

The technical details of two methods for measuring cell-mediated cytotoxicity against human tumor cells are described. The cytotoxic test in tissue culture microplates presents the advantage of using a low number of target and effector cells. The cytotoxic test with 51Cr allows a non subjective automatic reading. Both methods proved to be suitable to detect tumor specific antigens.

An assay measuring the stimulation of several types of bovine endothelial cells by growth factor(s) derived from cultured human tumor cells

In Vitro ◽  
1982 ◽  
Vol 18 (2) ◽  
pp. 99-107 ◽  
Author(s):  
J. V. Olander ◽  
J. C. Marasa ◽  
R. C. Kimes ◽  
G. M. Johnston ◽  
J. Feder
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Tumor Cells ◽  
Human Tumor ◽  
Human Tumor Cells ◽  
Bovine Endothelial Cells ◽  
Stimulation Of

Stimulation of TIMP-1 and metalloproteinase production in co-cultures of human tumor cells and human fibroblasts

1994 ◽  
Vol 78 (1-3) ◽  
pp. 133-140 ◽  
Author(s):  
Kazuki Nabeshima ◽  
Jun-ichi Kishi ◽  
Takao Kurogi ◽  
Naoto Komada ◽  
Hiroaki Kataoka ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Human Fibroblasts ◽  
Human Tumor ◽  
Human Tumor Cells ◽  
Stimulation Of
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