scholarly journals The biologic properties of recombinant human thrombopoietin in the proliferation and megakaryocytic differentiation of acute myeloblastic leukemia cells

Blood ◽  
1996 ◽  
Vol 88 (8) ◽  
pp. 3074-3082 ◽  
Author(s):  
I Matsumura ◽  
Y Kanakura ◽  
T Kato ◽  
H Ikeda ◽  
Y Horikawa ◽  
...  
Keyword(s):  
Growth Response ◽  
Acute Myeloblastic Leukemia ◽  
Leukemia Cells ◽  
Short Term ◽  
Megakaryocytic Differentiation ◽  
Biologic Effects ◽  
French American British ◽  
Short Term Culture ◽  
Recombinant Human Thrombopoietin

Thrombopoietin (TPO) is implicated as a primary regulator of megakaryopoiesis and thrombopoiesis. However, the biologic effects of TPO on human acute myeloblastic leukemia (AML) cells are largely unknown. To determine if recombinant human (rh) TPO has proliferation-supporting and differentiation-inducing activities in AML cells, 15 cases of AML cells that were exclusively composed of undifferentiated leukemia cells and showed growth response to rhTPO in a short-term culture (72 hours) were subjected to long-term suspension culture with or without rhTPO. Of 15 cases, rhTPO supported proliferation of AML cells for 2 to 4 weeks in 4 cases whose French-American-British subtypes were M0, M2, M4, and M7, respectively. In addition to the proliferation-supporting activity, rhTPO was found to induce AML cells to progress to some degree of megakaryocytic differentiation at both morphologic and surface-phenotypic level in 2 AML cases with M0 and M7 subtypes. The treatment of AML cells with rhTPO resulted in rapid tyrosine phosphorylation of the TPO-receptor, c-mpl, and STAT3 in all of cases tested. By contrast, the expression of erythroid/megakaryocyte-specific transcription factors (GATA-1, GATA-2, and NF-E2) was markedly induced or enhanced in only 2 AML cases that showed megakaryocytic differentiation in response to rhTPO. These results suggested that, at least in a fraction of AML cases, TPO could not only support the proliferation of AML cells irrespective of AML subtypes, but could also induce megakaryocytic differentiation, possibly through activation of GATA-1, GATA-2, and NF-E2.

scholarly journals Viability and Infectivity of Plasmodium vivax Gametocytes in Short-Term Culture

2021 ◽  
Vol 11 ◽  
Author(s):  
Glenda Quaresma Ramos ◽  
Djane Clarys Baia-da-Silva ◽  
Marcus Vinícius Guimarães Lacerda ◽  
Wuelton Marcelo Monteiro ◽  
Stefanie Costa Pinto Lopes
Keyword(s):  
Plasmodium Vivax ◽  
Cell Sorting ◽  
Short Term ◽  
Long Term Culture ◽  
Biological Aspects ◽  
New Strategies ◽  
Short Term Culture ◽  
Magnetic Activated Cell Sorting

The control and elimination of malaria caused by Plasmodium vivax both represent a great challenge due to the biological aspects of the species. Gametocytes are the forms responsible for the transmission of the parasite to the vector and the search for new strategies for blocking transmission are essential in a scenario of control and elimination The challenges in this search in regard to P. vivax mainly stem from the lack of a long-term culture and the limitation of studies of gametocytes. This study evaluated the viability and infectivity of P. vivax gametocytes in short-term culture. The samples enriched in gametocytes using Percoll (i), using magnetic-activated cell sorting (MACS®) (ii), and using non-enriched samples (iii) were evaluated. After the procedures, gametocytes were cultured in IMDM medium for up to 48 h. Cultured P. vivax gametocytes were viable and infectious for up to 48 h, however differences in viability and infectivity were observed in the samples after 12 h of culture in relation to 0 h. Percoll-enriched samples were shown to be viable in culture for longer intervals than those purified using MACS®. Gametocyte viability after enrichment procedures and short-term culture may provide new avenues in the development of methods for evaluating P. vivax TB.

scholarly journals Short-Term Growth Response to Growth Regulators In Roots of Zea Mays

1968 ◽  
Vol 21 (2) ◽  
pp. 201 ◽  
Author(s):  
DE Bottrill ◽  
JB Hanson
Keyword(s):  
Root Growth ◽  
Growth Response ◽  
Similar Degree ◽  
Dichlorophenoxyacetic Acid ◽  
Short Term ◽  
Irreversible Inhibition ◽  
Wide Range ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Indole 3 Acetic Acid

A study was made of the inhibition of root growth in Z. mays by 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,6-trichlorophenoxyacetic acid (2,4,6�T), and indole-3-acetic acid (IAA) over a wide range of concentrations. IAA and 2,4�D caused identical responses whereas the response to 2,4,6-T was different. Application of 2,4.D or IAA at concentrations of 4�5xl0-4M for 5 min resulted in an immediate reversible inhibition of root growth whereas long-term application gave an irreversible inhibition. The same concentration of 2,4,6-T caused a similar degree of inhibition of root growth, but only after an extended treatment, and the roots could then recover from the inhibition in the presence of the chemical.

scholarly journals The impact of post-warming culture duration on clinical outcomes of vitrified-warmed single blastocyst transfer cycles

2020 ◽  
Vol 47 (4) ◽  
pp. 312-318
Author(s):  
Ji Young Hwang ◽  
Jae Kyun Park ◽  
Tae Hyung Kim ◽  
Jin Hee Eum ◽  
HaengSeok Song ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Clinical Outcomes ◽  
Implantation Rate ◽  
Ongoing Pregnancy ◽  
Short Term ◽  
Ongoing Pregnancy Rate ◽  
Long Term Culture ◽  
Culture Duration ◽  
Short Term Culture

Objective: The objective of the study was to compare the effects of long-term and short-term embryo culture to assess whether there is a correlation between culture duration and clinical outcomes. Methods: Embryos were divided into two study groups depending on whether their post-warming culture period was long-term (20–24 hours) or short-term (2–4 hours). Embryo morphology was analyzed with a time-lapse monitoring device to estimate the appropriate timing and parameters for evaluating embryos with high implantation potency in both groups. Propensity score matching was performed to adjust the confounding factors across groups. The grades of embryos and blastoceles, morphokinetic parameters, implantation rate, and ongoing pregnancy rate were compared.Results: No significant differences were observed in the implantation rate or ongoing pregnancy rate between the two groups (56.3% vs. 67.9%, p=0.182; 47.3% vs. 53.6%, p=0.513). After warming, there were more expanded and hatching/hatched blastocysts in the long-term culture group than in the short-term culture group, but there was no significant between-group difference in embryo grade. Regarding pregnancy outcomes, the completion of re-expansion was faster in women who became pregnant than in those who did not for both culture durations (long-term: 2.19±0.63 vs. 4.11±0.81 hours, p=0.003; short-term: 1.17±0.29 vs. 1.94±0.76 hours, p=0.018, respectively). Conclusion: The outcomes of short-term culture and long-term culture were not significantly different in vitrified-warmed blastocyst transfer. Regardless of the post-warming culture time, the degree of blastocyst re-expansion 3–4 hours after warming is an important marker for embryo selection.

scholarly journals Cytologic characterization and significance of normal karyotypes in t(8;21) acute myeloblastic leukemia

Blood ◽  
1982 ◽  
Vol 59 (1) ◽  
pp. 171-178 ◽  
Author(s):  
R Berger ◽  
A Bernheim ◽  
MT Daniel ◽  
F Valensi ◽  
F Sigaux ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Sex Chromosome ◽  
Cytogenetic Study ◽  
Marrow Cell ◽  
Normal Karyotype ◽  
Acute Myeloblastic Leukemia ◽  
Chromosome Anomaly ◽  
Short Term ◽  
The Comparative Study ◽  
Short Term Culture

A cytologic and cytogenetic study of 10 cases of acute myeloblastic leukemia with maturation and t(8;21) translocation is reported. Despite a certain polymorphic appearance, the characteristic cytologic picture, consisting essentially of large myeloblasts with an abundant cytoplasma containing a large Auer rod, allowed the presence of the chromosome anomaly to be predicted. t(8;21) translocation was attended by the loss of a sex chromosome in 7 of 10 cases. The comparative study of mitoses using cytologic and cytogenetic techniques showed that cells exhibiting normal karyotypes were essentially erythroblasts. This finding suggests that the chromosome anomaly does not affect all the bone marrow cell lines. After short-term culture, the percentage of normal karyotype mitoses diminished, as did the number of mitoses in erythroblasts.

PARTIAL TRISOMY/MONOSOMY 6q IN FETAL CELLS AND CVS LONG-TERM CULTURE NOT PRESENT IN CVS SHORT-TERM CULTURE

1996 ◽  
Vol 16 (8) ◽  
pp. 741-748 ◽  
Author(s):  
R.-D. WEGNER ◽  
E. SCHRÖCK ◽  
M. OBLADEN ◽  
R. BECKER ◽  
M. STUMM ◽  
...  
Keyword(s):  
Partial Trisomy ◽  
Short Term ◽  
Long Term Culture ◽  
Fetal Cells ◽  
Short Term Culture

scholarly journals Cytologic characterization and significance of normal karyotypes in t(8;21) acute myeloblastic leukemia

Blood ◽  
1982 ◽  
Vol 59 (1) ◽  
pp. 171-178 ◽  
Author(s):  
R Berger ◽  
A Bernheim ◽  
MT Daniel ◽  
F Valensi ◽  
F Sigaux ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Sex Chromosome ◽  
Cytogenetic Study ◽  
Marrow Cell ◽  
Normal Karyotype ◽  
Acute Myeloblastic Leukemia ◽  
Chromosome Anomaly ◽  
Short Term ◽  
The Comparative Study ◽  
Short Term Culture

Abstract A cytologic and cytogenetic study of 10 cases of acute myeloblastic leukemia with maturation and t(8;21) translocation is reported. Despite a certain polymorphic appearance, the characteristic cytologic picture, consisting essentially of large myeloblasts with an abundant cytoplasma containing a large Auer rod, allowed the presence of the chromosome anomaly to be predicted. t(8;21) translocation was attended by the loss of a sex chromosome in 7 of 10 cases. The comparative study of mitoses using cytologic and cytogenetic techniques showed that cells exhibiting normal karyotypes were essentially erythroblasts. This finding suggests that the chromosome anomaly does not affect all the bone marrow cell lines. After short-term culture, the percentage of normal karyotype mitoses diminished, as did the number of mitoses in erythroblasts.

Growth response and radiation use efficiency in tomato exposed to short-term and long-term salinized soils

2015 ◽  
Vol 189 ◽  
pp. 139-149 ◽  
Author(s):  
S. De Pascale ◽  
A. Maggio ◽  
F. Orsini ◽  
C. Stanghellini ◽  
E. Heuvelink
Keyword(s):  
Growth Response ◽  
Radiation Use Efficiency ◽  
Short Term ◽  
Use Efficiency ◽  
Radiation Use

scholarly journals THE SHORT-TERM INCREASES OF IGF-I AND IGFBP-3 DURING GH TREATMENT PREDICT THE LONG-TERM GROWTH RESPONSE IN SHORT CHILDREN

1993 ◽  
Vol 33 ◽  
pp. S48-S48
Author(s):  
W F Blum ◽  
S Rosberg ◽  
M B Ranke ◽  
K Albertsson-Wikland
Keyword(s):  
Growth Response ◽  
Short Term ◽  
Gh Treatment ◽  
Short Children ◽  
Igf I ◽  
Igfbp 3

Conceptual short-term memory (CSTM) supports core claims of Christiansen and Chater

2016 ◽  
Vol 39 ◽  
Author(s):  
Mary C. Potter
Keyword(s):  
Working Memory ◽  
Rapid Serial Visual Presentation ◽  
Language Comprehension ◽  
Short Term Memory ◽  
Visual Presentation ◽  
Long Term Memory ◽  
Short Term ◽  
Term Memory ◽  
Use Of Knowledge

AbstractRapid serial visual presentation (RSVP) of words or pictured scenes provides evidence for a large-capacity conceptual short-term memory (CSTM) that momentarily provides rich associated material from long-term memory, permitting rapid chunking (Potter 1993; 2009; 2012). In perception of scenes as well as language comprehension, we make use of knowledge that briefly exceeds the supposed limits of working memory.

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