scholarly journals The synergistic necrohemorrhagic action of Clostridium perfringens perfringolysin and alpha toxin in the bovine intestine and against bovine endothelial cells

2013 ◽  
Vol 44 (1) ◽  
pp. 45 ◽  
Author(s):  
Stefanie Verherstraeten ◽  
Evy Goossens ◽  
Bonnie Valgaeren ◽  
Bart Pardon ◽  
Leen Timbermont ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Clostridium Perfringens ◽  
Alpha Toxin ◽  
Bovine Endothelial Cells

scholarly journals Alpha toxin from Clostridium perfringens induces proinflammatory changes in endothelial cells.

1997 ◽  
Vol 100 (3) ◽  
pp. 565-574 ◽  
Author(s):  
M Bunting ◽  
D E Lorant ◽  
A E Bryant ◽  
G A Zimmerman ◽  
T M McIntyre ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Clostridium Perfringens ◽  
Alpha Toxin

Culture of Arterial Endothelial Cells

1975 ◽  
Vol 34 (03) ◽  
pp. 825-839 ◽  
Author(s):  
Francois M Booyse ◽  
Bonnie J Sedlak ◽  
Max E Rafelson
Keyword(s):  
Endothelial Cells ◽  
Calf Serum ◽  
Intercellular Junctions ◽  
Cell Number ◽  
Population Doubling ◽  
Homogeneous Population ◽  
Bovine Endothelial Cells ◽  
Pinocytotic Vesicles ◽  
Arterial Endothelial Cells ◽  
Doubling Times

SummaryArterial endothelial cells were obtained from bovine aortae by mild treatment with collagenase and medium perfusion. These cells were cultured in RPMI-1640 medium containing 15 mM Hepes buffer and 35% fetal calf serum at pH 7.35. Essentially ah (90–95%) the effluent cells were viable and 80% of these cells attached to the substratum within 1 hour. Small patches of attached cells coalesced to form confluent monolayers in 3–5 days. Confluent monolayers of endothelial cells consisted of a homogeneous population of tightly packed, polygonal cells. Selected cultures were serially subcultured (trypsin-EDTA) for 12–14 months (30–35 passages) without any apparent change in morphology or loss of growth characteristics. Primary and three-month old (15 passages) cultures had population doubling times of 32–34 hours and 29–31 hours, respectively. These cells (primary and subcultures) did not require a minimum cell number to become established in culture. Bovine endothelial cells (primary, first, fifth and thirteenth passages) were characterized ultrastructurally by the presence of Weibel-Palade bodies, pinocytotic vesicles and microfilaments and immunologically by the presence of thrombosthenin-like contractile proteins and Factor VIII antigen. The intercellular junctions of post-confluent cultures stained specifically with silver nitrate. From these data, we concluded that identifiable endothelial cells could be obtained from bovine aortae and cultured and maintained for prolonged periods of time.

Vascular Smooth Muscle Cells Inhibit the Plasminogen Activators Secreted by Endothelial Cells

1985 ◽  
Vol 53 (02) ◽  
pp. 165-169 ◽  
Author(s):  
Walter E Laug
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Conditioned Medium ◽  
Vascular Smooth Muscle Cells ◽  
Inhibitory Activity ◽  
Muscle Cells ◽  
Plasminogen Activators ◽  
Bovine Endothelial Cells

SummaryTPure cultures of bovine endothelial cells (EC) produce and secrete large amounts of plasminogen activators (PA). Cocultivation of EC with vascular smooth muscle cells (SMC) resulted in a significant decrease of PA activities secreted by the EC, whereas the cellular PA activities remained unaffected. Secreted PA activities were absent in the growth medium as long as the SMC to EC ratio was 2:1 or higher. The PA inhibitory activity of the SMC was rapid and cell-to-cell contact was not necessary.The PA inhibitory activity was present in homogenates of SMC as well as in the medium conditioned by them but not in the extracellular matrix elaborated by these cells. Serum free medium conditioned by SMC neutralized both tissue type (t-PA) and urokinase like (u-PA) plasminogen activators. Gel electrophoretic analysis of SMC conditioned medium followed by reverse fibrin autography demonstrated PA inhibitory activities in the molecular weight (Mr) range of 50,000 to 52,000 similar to those present in media conditioned by bovine endothelial cells or fibroblasts. Regular fibrin zymography of SMC conditioned medium incubated with u-PA or t-PA revealed the presence of a component with a calculated approximate Mr of 45,000 to 50,000 which formed SDS resistant complexes with both types of PA.These data demonstrate that vascular SMC produce and secrete (a) inhibitor(s) of PAs which may influence the fibrinolytic potential of EC.

Envelope glycoprotein of avian hemangioma retrovirus induces a thrombogenic surface on human and bovine endothelial cells.

1990 ◽  
Vol 64 (8) ◽  
pp. 4029-4032 ◽  
Author(s):  
N Resnick-Roguel ◽  
A Eldor ◽  
H Burstein ◽  
E Hy-Am ◽  
I Vlodavsky ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Envelope Glycoprotein ◽  
Bovine Endothelial Cells

scholarly journals Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells.

1994 ◽  
Vol 269 (31) ◽  
pp. 19897-19903
Author(s):  
S. Hara ◽  
A. Miyata ◽  
C. Yokoyama ◽  
H. Inoue ◽  
R. Brugger ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Molecular Cloning ◽  
Bovine Endothelial Cells ◽  
Prostacyclin Synthase

Secretory mechanism of immunoreactive endothelin in cultured bovine endothelial cells

1989 ◽  
Vol 160 (1) ◽  
pp. 93-100 ◽  
Author(s):  
Toshiaki Emori ◽  
Yukio Hirata ◽  
Kazuki Ohta ◽  
Masayoshi Shichiri ◽  
Fumiaki Marumo
Keyword(s):  
Endothelial Cells ◽  
Bovine Endothelial Cells

scholarly journals Perfringolysin O Expression in Clostridium perfringens Is Independent of the Upstream pfoR Gene

2002 ◽  
Vol 184 (7) ◽  
pp. 2034-2038 ◽  
Author(s):  
Milena M. Awad ◽  
Julian I. Rood
Keyword(s):  
Escherichia Coli ◽  
Homologous Recombination ◽  
Gene Product ◽  
Structural Gene ◽  
Clostridium Perfringens ◽  
Deletion Mutant ◽  
Gas Gangrene ◽  
Alpha Toxin ◽  
Clostridial Myonecrosis ◽  
Perfringolysin O

ABSTRACT The pathogenesis of Clostridium perfringens-mediated gas gangrene or clostridial myonecrosis involves the extracellular toxins alpha-toxin and perfringolysin O. Previous studies (T. Shimizu, A. Okabe, J. Minami, and H. Hayashi, Infect. Immun. 59:137-142, 1991) carried out with Escherichia coli suggested that the perfringolysin O structural gene, pfoA, was positively regulated by the product of the upstream pfoR gene. In an attempt to confirm this hypothesis in C. perfringens, a pfoR-pfoA deletion mutant was complemented with isogenic pfoA+ shuttle plasmids that varied only in their ability to encode an intact pfoR gene. No difference in the ability to produce perfringolysin O was observed for C. perfringens strains carrying these plasmids. In addition, chromosomal pfoR mutants were constructed by homologous recombination in C. perfringens. Again no difference in perfringolysin O activity was observed. Since it was not possible to alter perfringolysin O expression by mutation of pfoR, it was concluded that the pfoR gene product is unlikely to have a role in the regulation of pfoA expression in C. perfringens.

scholarly journals Enhanced n-3 phospholipid content reduces inflammatory responses in bovine endothelial cells

2012 ◽  
Vol 95 (12) ◽  
pp. 7137-7150 ◽  
Author(s):  
G.A. Contreras ◽  
S.A. Mattmiller ◽  
W. Raphael ◽  
J.C. Gandy ◽  
L.M. Sordillo
Keyword(s):  
Endothelial Cells ◽  
Inflammatory Responses ◽  
Phospholipid Content ◽  
Bovine Endothelial Cells
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