scholarly journals Comparative genomic analysis reveals significant enrichment of mobile genetic elements and genes encoding surface structure-proteins in hospital-associated clonal complex 2 Enterococcus faecalis

2011 ◽  
Vol 11 (1) ◽  
pp. 3 ◽  
Author(s):  
Margrete Solheim ◽  
Mari C Brekke ◽  
Lars G Snipen ◽  
Rob JL Willems ◽  
Ingolf F Nes ◽  
...  
Keyword(s):  
Surface Structure ◽  
Enterococcus Faecalis ◽  
Clonal Complex ◽  
Genomic Analysis ◽  
Mobile Genetic Elements ◽  
Comparative Genomic Analysis ◽  
Comparative Genomic ◽  
Genetic Elements ◽  
Genes Encoding ◽  
Significant Enrichment

scholarly journals Identification of Genetic Bases ofVibrio fluvialisSpecies-Specific Biochemical Pathways and Potential Virulence Factors by Comparative Genomic Analysis

2014 ◽  
Vol 80 (6) ◽  
pp. 2029-2037 ◽  
Author(s):  
Xin Lu ◽  
Weili Liang ◽  
Yunduan Wang ◽  
Jialiang Xu ◽  
Jun Zhu ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Genomic Analysis ◽  
Comparative Genomic Analysis ◽  
Comparative Genomic ◽  
Type Vi Secretion System ◽  
Biochemical Tests ◽  
Regulatory Pathways ◽  
Vibrio Fluvialis ◽  
Genes Encoding ◽  
Genetic Mechanisms

ABSTRACTVibrio fluvialisis an important food-borne pathogen that causes diarrheal illness and sometimes extraintestinal infections in humans. In this study, we sequenced the genome of a clinicalV. fluvialisstrain and determined its phylogenetic relationships with otherVibriospecies by comparative genomic analysis. We found that the closest relationship was betweenV. fluvialisandV. furnissii, followed by those withV. choleraeandV. mimicus. Moreover, based on genome comparisons and gene complementation experiments, we revealed genetic mechanisms of the biochemical tests that differentiateV. fluvialisfrom closely related species. Importantly, we identified a variety of genes encoding potential virulence factors, including multiple hemolysins, transcriptional regulators, and environmental survival and adaptation apparatuses, and the type VI secretion system, which is indicative of complex regulatory pathways modulating pathogenesis in this organism. The availability ofV. fluvialisgenome sequences may promote our understanding of pathogenic mechanisms for this emerging pathogen.

scholarly journals Pathogenic Determinants of the Mycobacterium kansasii Complex: An Unsuspected Role for Distributive Conjugal Transfer

2021 ◽  
Vol 9 (2) ◽  
pp. 348
Author(s):  
Florian Tagini ◽  
Trestan Pillonel ◽  
Claire Bertelli ◽  
Katia Jaton ◽  
Gilbert Greub
Keyword(s):  
Genomic Analysis ◽  
Comparative Genomic ◽  
Mycobacterium Kansasii ◽  
Type Vii Secretion ◽  
A Genome ◽  
Genes Encoding ◽  
Associated Proteins ◽  
Immunosuppressed Patients ◽  
Type Vii Secretion System ◽  
Clinical Records

The Mycobacterium kansasii species comprises six subtypes that were recently classified into six closely related species; Mycobacterium kansasii (formerly M. kansasii subtype 1), Mycobacterium persicum (subtype 2), Mycobacterium pseudokansasii (subtype 3), Mycobacterium ostraviense (subtype 4), Mycobacterium innocens (subtype 5) and Mycobacterium attenuatum (subtype 6). Together with Mycobacterium gastri, they form the M. kansasii complex. M. kansasii is the most frequent and most pathogenic species of the complex. M. persicum is classically associated with diseases in immunosuppressed patients, and the other species are mostly colonizers, and are only very rarely reported in ill patients. Comparative genomics was used to assess the genetic determinants leading to the pathogenicity of members of the M. kansasii complex. The genomes of 51 isolates collected from patients with and without disease were sequenced and compared with 24 publicly available genomes. The pathogenicity of each isolate was determined based on the clinical records or public metadata. A comparative genomic analysis showed that all M. persicum, M. ostraviense, M innocens and M. gastri isolates lacked the ESX-1-associated EspACD locus that is thought to play a crucial role in the pathogenicity of M. tuberculosis and other non-tuberculous mycobacteria. Furthermore, M. kansasii was the only species exhibiting a 25-Kb-large genomic island encoding for 17 type-VII secretion system-associated proteins. Finally, a genome-wide association analysis revealed that two consecutive genes encoding a hemerythrin-like protein and a nitroreductase-like protein were significantly associated with pathogenicity. These two genes may be involved in the resistance to reactive oxygen and nitrogen species, a required mechanism for the intracellular survival of bacteria. Three non-pathogenic M. kansasii lacked these genes likely due to two distinct distributive conjugal transfers (DCTs) between M. attenuatum and M. kansasii, and one DCT between M. persicum and M. kansasii. To our knowledge, this is the first study linking DCT to reduced pathogenicity.

Sign in / Sign up

Export Citation Format

Share Document