In vitro evaluation of Pandanus amaryllifolius ethanol extract for induction of cell death on non-hormone dependent human breast adenocarcinoma MDA-MB-231 cell via apoptosis

The compound 3-{[(2,3-Dichlorophenyl)amino]methyl}-5-(furan-2-ylmethylidene)-1,3-thiazolidine-2,4-dione has been designed, synthesized, and screened for its in vitro antibreast cancer activity, using human breast adenocarcinoma cell lines (MCF-7) and in vitro anti-inflammatory activity. By hemolysis assay, it showed that it has a nonhemolytic and nontoxic effect on human blood cell. The title compound 5, subjected to in vitro activities, showed that it is cytotoxic with an IC50 of 42.30 µM and a good anti-inflammatory agent. The docking results against cyclin dependent kinase 2 (CDK2) (PDB ID: 3QQK) gave insights on its inhibitory activity.

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Enhancement of docetaxel-treated MCF-7 cell death by 900-MHz radiation

Open Life Sciences ◽

10.2478/s11535-013-0143-z ◽

2013 ◽

Vol 8 (4) ◽

pp. 357-365

Author(s):

Marianna Trebuňová ◽

Galina Laputková ◽

Imrich Géci ◽

Igor Andrašina ◽

Ján Sabo

Keyword(s):

Cell Culture ◽

Cell Death ◽

Electromagnetic Field ◽

High Frequency ◽

Input Power ◽

Breast Adenocarcinoma ◽

Human Breast ◽

Human Breast Adenocarcinoma ◽

High Frequency Electromagnetic Field ◽

Mcf 7

AbstractThe aim of the study was to investigate the effect of high-frequency electromagnetic field of 900 MHz at 8 W input power on metabolic activity of human breast adenocarcinoma MCF-7 cells. With the aid of the colorimetric MTT assay, it was shown that there is significant change in cell culture survival exposed to docetaxel in field-free conditions in comparison with cells treated with docetaxel simultaneously exposed to high-frequency electromagnetic field.

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Photodynamic activity of 2,6-diiodo-3,5-dithienylvinyleneBODIPYs and their folate-functionalized chitosan-coated Pluronic® F-127 micelles on MCF-7 breast cancer cells

Journal of Porphyrins and Phthalocyanines ◽

10.1142/s1088424619501773 ◽

2020 ◽

Vol 24 (05n07) ◽

pp. 973-984

Author(s):

Nthabeleng Molupe ◽

Balaji Babu ◽

David O. Oluwole ◽

Earl Prinsloo ◽

Lizhi Gai ◽

...

Keyword(s):

Breast Cancer Cells ◽

Drug Delivery Systems ◽

Quantum Yields ◽

Breast Adenocarcinoma ◽

Human Breast ◽

Human Breast Adenocarcinoma ◽

Viable Cells ◽

Photodynamic Activity ◽

Mcf 7

A 2,6-diiodo-3,5-dithienylvinyleneBODIPY dye was prepared and encapsulated with folate-chitosan capped Pluronic[Formula: see text] F-127 to provide drug delivery systems for photodynamic therapy (PDT). Moderately enhanced singlet oxygen quantum yields were observed for the dye encapsulation complexes in water. The in vitro dark cytotoxicity and photodynamic activity were investigated on the human breast adenocarcinoma (MCF-7) cell line. Minimal dark cytotoxicity was observed for the BODIPY dyes in 5% DMSO and when encapsulated in folate-functionalized chitosan-coated Pluronic[Formula: see text] F-127 micelles, since the cell viability values are consistently greater than 80% over the 0-40 [Formula: see text] concentration range. Upon irradiation of the samples, significant cytocidal activity was observed for the encapsulation complex of a 2,6-diiodo-8-dimethylaminophenyl-3,5-dithienylvinyleneBODIPY dye with less than 50% viable cells observed at concentrations [Formula: see text].

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