scholarly journals Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand

2012 ◽  
Vol 11 (1) ◽  
Author(s):  
Kanungnit Congpuong ◽  
Aungkana SaeJeng ◽  
Rungniran Sug-aram ◽  
Supannee Aruncharus ◽  
Ampai Darakapong ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
North West ◽  
Expert Microscopy

Tick-borne fever caused by Anaplasma phagocytophilum in Germany

2012 ◽  
Vol 40 (02) ◽  
pp. 101-106 ◽  
Author(s):  
M. Nieder ◽  
C. Silaghi ◽  
D. Hamel ◽  
K. Pfister ◽  
R. Schmäschke ◽  
...  
Keyword(s):  
Real Time ◽  
Milk Production ◽  
Real Time Pcr ◽  
Anaplasma Phagocytophilum ◽  
Clinical Findings ◽  
Immunofluorescence Assay ◽  
High Fever ◽  
West Germany ◽  
Nasal Discharge ◽  
North West

SummaryFour cows from North-West Germany have been diagnosed with tickborne fever (TBF) based on the demonstration of morulae in neutrophilic granulocytes in their blood smears, positive signals in real-time PCR specific for Anaplasma phagocytophilum using DNA extracted from their buffy coats, and demonstration of specific antibodies in their sera using a commercially available immunofluorescence assay. Clinical findings included high fever, decreased milk production, lower limb edema with stiff walking, eye and nasal discharge, and depression. These signs developed about a week after the animals had been brought to the pasture for the first time in their life. All cows recovered after 5–15 days, although DNA of A. phagocytophilum could be detected by real-time PCR up to 6 weeks after onset of the disease. Considering the known prevalences of A. phagocytophilum in ticks in Germany and its detection in dogs and horses, we think that underdiagnosing of TBE in cattle is highly likely. Therefore TBF should be taken into account as differential diagnosis in case of high fever and/or a sudden decrease in milk production in pastured animals.

scholarly journals High-Resolution Melting Analysis as an Appropriate Method to Differentiate between Fasciola hepatica and F. gigantica

2019 ◽  
Author(s):  
Ahmad HOSSEINI-SAFA ◽  
Mohammad Bagher ROKNI ◽  
Sayed Hussain MOSAWI ◽  
Peyman HEYDARIAN ◽  
Hakim AZIZI ◽  
...  
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Real Time Pcr ◽  
High Resolution Melting ◽  
Fasciola Hepatica ◽  
Pcr Amplification ◽  
Epidemiological Survey ◽  
North West ◽  
Melting Analysis ◽  
Fasciola Spp

Background: Fasciolosis is a shared disease between humans and livestock caused by hepatic trematodes; Fasciola hepatica and F. gigantica. Differentiate between the two species of this genus is essential. High-Resolution Melting (HRM) Analysis represents a new approach to this issue. This method can be performed right after termination of Real-Time PCR. This technique has not been used for identification of adult F. hepatica and F. gigantica genotypes. The aim of this study was to determine Fasciola species by using HRM in isolates taken from Iran, respectively. Methods: Ninety-three Fasciola spp. samples were collected from infected slaughtered animals in different regions of Iran, including North West (Ardebil Province) and South East (Zahedan Province) during 2016. Genomic DNA from the samples was extracted using a DNA extraction kit and then after Real-Time PCR amplification, HRM was done. Results: Overall, 59 and 34 isolates were identified as F. hepatica and F. gigantica, respectively. The percentages of each species from animals were as follows: sheep (F. hepatica, 80.39% and F. gigantica, 19.61%), cattle (F. hepatica, 42.85% and F. gigantica, 57.15%). Conclusion: HRM technique developed in the present study is a powerful, rapid and sensitive technique for epidemiological survey and molecular identification between F. hepatica and F. gigantica.

Feldvalidierung einer real-time PCR zum Nachweis von Mycoplasma hyopneumoniae in Nasentupfermaterial von lebenden Schweinen

2005 ◽  
Vol 147 (9) ◽  
pp. 373-379 ◽  
Author(s):  
F. Zeeh ◽  
P. Kuhnert ◽  
R. Miserez ◽  
M. G. Doherr ◽  
W. Zimmermann
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Mycoplasma Hyopneumoniae

Nutzen des hoch-sensitiven real-time-PCR HBV Tests (TaqMan) zur Prädiktion der Resistenzentwicklung bei Lamivudin-Langzeittherapie

2010 ◽  
Vol 48 (08) ◽  
Author(s):  
A Brodzinski ◽  
F van Bömmel ◽  
B Fülöp ◽  
B Schlosser ◽  
M Biermer ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr

Nachweis des porzinen Circovirus Typ 2 und des Torque-Teno-Sus-Virus 1 und 2 in Samenproben von Ebern einer österreichischen Besamungsstation

2011 ◽  
Vol 39 (04) ◽  
pp. 201-204
Author(s):  
A. Griessler ◽  
E. Pirker ◽  
H. Söllner ◽  
J. Segalés ◽  
T. Kekarainen ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Klinische Relevanz ◽  
Torque Teno Sus Virus

Zusammenfassung Gegenstand und Ziel: Das porzine Circovirus Typ 2 (PCV-2) und das Torque-teno-Sus-Virus (TTSuV) sind in schweineproduzierenden Ländern häufig nachzuweisen. Beide Erreger können sowohl horizontal als auch vertikal übertragen werden und Ebersamen könnte ein wichtiges Übertragungsmedium darstellen. Ziel der Studie war die Abklärung der Prävalenz dieser beiden Viren in Samenproben von Ebern. Material und Methoden: Von 100 Ebern einer Besamungsstation wurde jeweils eine Samenprobe mittels quantitativer Real-Time-PCR auf PCV-2 und mittels konventioneller PCR auf TTSuV-1 und TTSuV-2 untersucht. Ergebnisse: Nur bei einem Eber der Rasse Piétrain war ein positives PCV-2-Resultat festzustellen. TTSuV-1 ließ sich in vier Samenproben, TTSuV-2 in fünf Proben nachweisen. Ein Eber wies eine Koinfektion mit beiden TTSuV-Genotypen auf. Alle TTSuV-positiven Proben stammten von Piétrain-Ebern. Schlussfolgerung und klinische Relevanz: In der vorliegenden Studie wurde erstmals in Österreich TTSuV im Samen nachgewiesen. Die Prävalenz sowohl von TTSuV als auch von PCV-2 war gering. Die klinische Relevanz einer gleichzeitigen Kontamination des Samens mit beiden Viren ist nicht klar.

Vergleich von Ovarialendometriose, Ovarialkarzinom und benignen Ovarien mittels Microarray-Untersuchungen und Real-time-PCR

2005 ◽  
Vol 66 (S 1) ◽  
Author(s):  
D Hornung ◽  
C Banz ◽  
U Ungethüm ◽  
RJ Kuban ◽  
H Xu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr
Sign in / Sign up

Export Citation Format

Share Document