scholarly journals Detection of mixed infection level of Plasmodium falciparum and Plasmodium vivax by SYBR Green I-based real-time PCR in North Gondar, north-west Ethiopia

2014 ◽  
Vol 13 (1) ◽  
Author(s):  
Addimas Tajebe ◽  
Gabriel Magoma ◽  
Mulugeta Aemero ◽  
Francis Kimani
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Vivax ◽  
Real Time ◽  
Mixed Infection ◽  
Real Time Pcr ◽  
Sybr Green ◽  
Sybr Green I ◽  
Infection Level ◽  
North West ◽  
North West Ethiopia

scholarly journals Management of uncomplicated malaria in private health facilities in North-West Ethiopia: a clinical audit of current practices

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Mesele Damte Argaw ◽  
Thandisizwe Redford Mavundla ◽  
Kassa Daka Gidebo
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Vivax ◽  
Clinical Audit ◽  
Malaria Diagnosis ◽  
Public Private Partnership ◽  
Diagnosis And Treatment ◽  
Private Partnership ◽  
North West ◽  
Minor Error ◽  
North West Ethiopia

Abstract Background Malaria is one of the leading public health problems in sub-Saharan Africa that contributes to significant patient morbidity and mortality. The aim of the study was to investigate adherence to malaria diagnosis and treatment guidelines by private health sector providers and compare their performance against the public private partnership (PPP) status. Methods A facility-based retrospective clinical audit was conducted between October 2016 and January 2017 in 11 medium clinics in the West Gojjam zone of the Amhara Region, North-west Ethiopia. Data was extracted from patient medical records using pretested data abstraction forms. Descriptive statistics were employed to present the findings and adherence of health workers against the national and international standards were classified as ideal, acceptable, minor error and major error for both malaria diagnosis and treatment. A chi-square (X2) test was used to test for a statistically significant relationship after the data had been categorized using public private partnership status at P < 0.05. Results One thousand six hundred fifty clinical files were audited. All malaria suspected patients were investigated either with microscopy or rapid diagnostics test (RDT) for parasitological confirmation. The proportion of malaria treated cases was 23.7% (391/1650). Of which 16.6% (274/1650) were uncomplicated, 3.69% (61 /1650) were severe and complicated and the rest 3.39% (56/1650) were clinical diagnosed malaria cases. And the malaria parasite positivity rate was 20.30% (335/1650). All malaria suspected patients were not investigated with ideal malaria diagnosis recommendations; only 19.4% (320/1650) were investigated with acceptable malaria diagnosis (public private partnership (PPP) 19.4%; 176/907; and non-public private partnership (NPPP) 19.38%; 144/743, X2 (1) = 0.0With regards to treatments of malaria cases, the majority 82.9% of Plasmodium vivax cases were managed with ideal recommended treatment (X2 (1) = 0.35, P = 0.55); among Plasmodium falciparum, mixed (Plasmodium falciparum and Plasmodium vivax). Conclusion The clinical audit revealed that the majority of malaria patients had received minor error malaria diagnostic services. In addition, only one fifth of malaria patients had received ideal malaria treatment services. To understand the reasons for the low levels of malaria diagnosis and treatment adherence with national guidelines, a qualitative exploratory descriptive study is recommended.

Fast and sensitive quantitative detection of HIV DNA in whole blood leucocytes by SYBR green I real-time PCR assay

2007 ◽  
Vol 21 (5-6) ◽  
pp. 368-378 ◽  
Author(s):  
Anna Casabianca ◽  
Caterina Gori ◽  
Chiara Orlandi ◽  
Federica Forbici ◽  
Carlo Federico Perno ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Whole Blood ◽  
Sybr Green ◽  
Sybr Green I ◽  
Quantitative Detection ◽  
Pcr Assay ◽  
Hiv Dna

scholarly journals Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRs

2017 ◽  
Vol 61 (4) ◽  
pp. 427-432 ◽  
Author(s):  
Agnieszka Kędrak-Jabłońska ◽  
Sylwia Budniak ◽  
Marek Krupa ◽  
Anna Szczawińska ◽  
Monika Reksa ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Real Time ◽  
Real Time Pcr ◽  
Biological Samples ◽  
High Specificity ◽  
Sybr Green ◽  
Sybr Green I ◽  
Taqman Probe ◽  
Rdna Gene ◽  
Intercalating Dye

AbstractIntroduction:The aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene ofListeriaspp. and thehlyA gene ofListeria monocytogenesin biological samples of the liver, brain, and blood.Material and Methods:Five strains ofL. monocytogenesand single strains of each speciesL. ivanovii,L. innocua,L. grayi,L. welshimeri,andL. seeligeriwere used for the experiments. Additionally, five strains of other species of bacteria were used for evaluation of the specificity of tests. In the first stage of the study SYBR Green I real-time PCRs, one allowing detection of the 23S rDNA gene and two based on the amplification thehlyA gene, were performed. In the next part, three TaqMan probe-based real-time PCRs allowing confirmation of belonging toListeriaspp. andL. monocytogeneswere conducted.Results:The observation of amplification curves in real-time PCRs enabled the detection of both genes. A high regression coefficient of 0.99 was found for all reactions. Specific amplification products were obtained for the 23S rDNA andhlyA genes which confirm their belonging toListeriaspp. andL. monocytogenes, respectively. Other microbial species did not reveal real-time PCR products.Conclusion:Both real-time PCR methods for the detection ofListeriaspp. andL. monocytogenesin biological samples demonstrated a significant sensitivity and high specificity.

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